Working group session:
Germplasm and Genetic Stocks
Presentation type:
oral
Authors:
Cho, Seungho; Yanai, Giane; Schwarz, John; Curtis, Amy; Butruille, Marymar
Presenter:
Cho, Seungho
Correspondent:
Abstract:
SNP markers representing whole Gossypium hirsutum genome were developed in Taqman MGB format and validated their functionality using diverse germplasm lines including commercial inbred lines. Template sequences of total number of 96 Taqman SNPs were acquired from EST sequencing conducted in Monsanto Company. Based on in silico comparative mapping approach using public D genome sequence, these 96 template sequences were expected to be distributed across 26 chromosomes in both A and D genomes. Polymorphism information content value of these 96 Taqman SNPs ranged between 0.310 and 0.375. Genetic diversity among germplasm lines was revealed based on SNP allele calls and marker-based genetic diversity was also compared to phenotype based approach. The result showed that SNP marker-based genetic diversity was informative and can assist with variety identification purposes such as enforcement of intellectual property rights or determination of genetic conformity resolution of essentially derived variety (EDV) or in the examination of Distinctness. Monsanto Company is releasing these 96 SNP markers to CottonGEN (http://www.cottongen.org/) for cotton genomics community to use them as a genetic diversity assay tool.