Gene isolation and its identification of salinity stress on G.hirsutum L.

Germplasm and Genetic Stocks

oral

Ye, Wuwei

Ye, Wuwei

Ye, Wuwei

Gene isolation and its identification of salinity stress on G.hirsutum L. YE Wu-wei*, WANG Jun-juan，WANG De-long，YIN Zu-jun， FAN Wei-li，WANG Shuai，Song Liyan, Zhang Lina, Zhou Kai, LU Xu-ke (State Key Laboratory of Cotton Biology / Institute of Cotton Research of CAAS, Anyang455000, Henan, China. Emai:yew15@hotmail.com;Tel:86-372-2562283;0086-13803724991) Soil salinization has become a serious global problem affecting the agricultural development and the ecological environment. Salinity，as one of the most important abiotic stresses in the world, severely limits the production of crop. Saline-alkali land in our country is widely distributed with the character of multi types and serious salt-deposition. In order to carry out the utilization of saline-alkali land efficiently, it is necessary to develop the agriculture on the saline-alkali land.Cotton, as a pioneer crop in saline-alkali land, should be paid more efforts to conduct the mechanism research of salt-tolerace and to breed new varieties of salt-tolerant. Cotton, the major cash crop in China, is playing a crucial role in national economic development. China, with less cultivated lands and more people, faces the contradiction between food and cotton, which seriously affects the cultivation and production of cotton. Therefore, It is an effective way to farm saline land and to enhance the sustainable agricultural development by develop the salinity-tolerant varieties of cotton. Identification of salinity-tolerance also plays a vital role on cotton breeding. The abiotic-tolerant identification methods used before, mainly based on morphological characters, were usually restricted for time-wasting and labor-costing, environment influence, and seasonal restrictions. A new set of preliminary methods system, called SSR multi-markers salinity-identification method, was initially established to identify salinity tolerance of cotton by the standardization of the whole process of seedling nursing, DNA extraction, PCR amplification, amplification products detecting, and marker-combination. Another 11 materials were used to testify this method, which showed the coincidence of 90.91% in consistence with the identification result of 0.4%NaCl identification method. This study showed that the multi-markers identification method was proved to be used to assist identify the salinity tolerance of cotton germplasm. Seven salt-tolerance related genes, H+-pyrophosphatase gene and S-adenosylmethionine synthetase gene and others, were cloned from the salt-tolerance material on Gossypium hirsutum, which were named GhVP and GhSAMS, respectively. The bioinformatics analysis and their transformed accessions were tested and identified. Key word: Cotton germplasm；identification；salinity;