Working group session:
Functional Genomics
Presentation type:
poster
Authors:
Cai, Xixi; Qin, Yiding; Xu, Yingying; Huang, Xuejiao; Xu, Xingxing; Huang, Jie ; Xie, Xiaoling; Wang, Baohua
Presenter:
Wang, Baohua
Correspondent:
Wang, Baohua
Abstract:
In this research, methylation-sensitive amplification polymorphism (MSAP) method based on capillary electrophoresis was used to analyze DNA methylation level in a cotton hybrid CCRI 29, so as to discuss the molecular mechanism of salt tolerance in cotton. Totally 24 primer combinations were adopted and 69.2 and 56.7 methylated CCGG loci per primer combination were detected in CCRI 29 treated with 0.4% of salt water and control respectively, which were significantly different. Comparing to water control, 52.6% of the loci under salt stress showed increased methylation level, meaning hypermethylation happened; whereas 19.7% of the loci showed decreased methylation level, meaning hypomethylation happened; the hypermethylation rate is significantly higher than that of hypomethylation. The results showed that all kinds of variation of DNA methylation happened in CCRI 29 under salt stress, including hypermethylation, hypomethylation and other patterns; hypermethylation happened at a significantly higher rate than that of hypomethylation. DNA methylation level significantly increased under salt stress in CCRI 29, suggesting that CCRI 29 might have the potential mechanism of increasing methylation level when responding to salt stress. The increase of overall level of DNA methylation in cotton genome might play an important role in tolerance to salt stress in cotton. Preliminary cloning and analysis of methylated DNA sequences showed that some ATP synthase subunit genes maintained the same methylation level after salt stress, so their expression should not be inhibited by DNA methylation, which might be part of the reason that CCRI 29 is salt tolerant and can maintain normal growth after salt stress in a certain period.