Working group session:
Germplasm and Genetic Stocks
Zakirova, Darya; Tashpulatova, Sabokhat; Radjapov, Farkhod; Egamberdiev, Sharof; Salakhutdinov, Ilkhom; Abdurakhmonov, Ibrokhim; Buriev, Zabardast; Abdullaev, Alisher
The aim of our study is development of molecular genetic fingerprints of commercial cotton varieties cultivated in Uzbekistan using SSR markers. Thirty seven samples from Upland cotton germplasm collection of the Centre of genomics and bioinformatics were genotyped using 185 primer pairs. From 185 SSR of 38 yielded polymorphic loci suitable to create molecular profiles of the studying samples. The minimum and maximum number of loci varied from 2 to 7 respectively, with average of 2,81 loci per marker. Having assessed the content of polymorphic information content (PIC) of the markers, we found that the minimum and maximum value was 0,051 and 0,609, respectively with a mean value of 0,358. The lowest and highest value expected heterozygosity value (He) ranged from 0,052 to 0,678 with a mean value of 0,421. Further phylogenetic analysis using microsatellite data and UPGMA (unweighted pair group method with arithmetic mean) grouped 37 cotton varieties to 6 clasters. The most distant from others samples were 6 and 12. Varieties Namangan 102, Namangan 34, AN 16 also formed a separate cluster, as well as the line C 8271. Results of the study revealed narrow genetic base of selected commercial cotton cultivars. Identified 38 SSR markers are highly polymorphic, and able to discriminate all tested cotton cultivars. These markesr could be used as a DNA fingerprinting set for local cotton cultivars.