g.raimondii_cottongen_reftransV1_0000434, g.raimondii_cottongen_reftransV1_0000434 (contig) Gossypium raimondii

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: A0A072UCZ2_MEDTR (Neutral alpha-glucosidase OS=Medicago truncatula GN=MTR_5g026610 PE=3 SV=1)

HSP 1 Score: 957.207 bits (2473), Expect = 0.000e+0
Identity = 462/598 (77.26%), Postives = 519/598 (86.79%), Query Frame = 1

Query:   52 RFNPSPPSSTSKFLFRKKSYSKKLTGRRLISKMTDSE--VKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVM-SNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYS 1836
            R + + P  +S    R+K + +KL     ISKM + E  V + ++D   GKMIF+PIL DGVFRFDCS NDR+A YPS+SF+N+K R+ P+  ++K PSY P+FEC L QQ+V+LE P GTS YGTGEVSGQLERTGKRVFTWNTDAWGYGP T+SLYQSHPWVLAVLPNGEALGILADTTRRCEIDLR E  I+FI+  S+PVITFGPF+SPT VL+SLS AIGTVFMPPKWSLGY QCRWSY S +RVLE+++ FREK IPCDVIWMDIDYMDGFRCFTFDKERF DPKSLV+ LH  GFK IWMLDPGIKQE+GYFVYDSGS++DVW+QKADG  FVGDVWPGPCVFPD+TQSK RAWWANLVKDF+SNGVDGIWNDMNEPA+FK VTKTMPESN+HRGD ELGGCQ+H+ YHNVYG+LMARSTYEGM LA+  +RPFVLTRAGF GSQRYAATWTGDNLS WEHL MSISM+LQLGLSGQPLSGPDIGGFAGNATP+LFGRW+G G++FPFCRGHSE GT DHEPWSFG+ECEEVCRLALKRRYRLIP IYTLFY AHT+G PVATP FFADP DP+LR LE+ FLLGP+LVY+
Sbjct:   37 RLHRNTPFLSSIITLRRKRFIEKL-----ISKMANYEGQVSSGSTDVRTGKMIFEPILNDGVFRFDCSLNDRDAAYPSISFVNSKDRETPITGTHKVPSYTPTFECLLEQQVVQLELPVGTSLYGTGEVSGQLERTGKRVFTWNTDAWGYGPGTSSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRKESTIRFIAPSSYPVITFGPFASPTEVLISLSKAIGTVFMPPKWSLGYQQCRWSYLSDQRVLEVAKTFREKSIPCDVIWMDIDYMDGFRCFTFDKERFRDPKSLVESLHYSGFKGIWMLDPGIKQEKGYFVYDSGSENDVWVQKADGTAFVGDVWPGPCVFPDYTQSKVRAWWANLVKDFVSNGVDGIWNDMNEPAVFKAVTKTMPESNVHRGDGELGGCQNHSFYHNVYGLLMARSTYEGMKLANENRRPFVLTRAGFSGSQRYAATWTGDNLSTWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGSLFPFCRGHSEAGTTDHEPWSFGEECEEVCRLALKRRYRLIPLIYTLFYFAHTKGIPVATPTFFADPTDPSLRKLENSFLLGPVLVYA 629          

HSP 2 Score: 437.958 bits (1125), Expect = 1.001e-129
Identity = 223/335 (66.57%), Postives = 263/335 (78.51%), Query Frame = 2

Query: 2567 SFFILPCFTY*QFIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDG-SGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRL 3568
            SF + P   Y       S   + G DKL+  LPKGIWL FDF D+HPDLPALYL+GG IIP G P QHVGE+NP D+LTL+VALDE GKA+G LFEDDGDGY FT G YLLTHY A+L+ + VTV V +TEG WKRP RRLH+QLL+G  AMLD WG DGE L + +PSE EVSKL+S+S++ ++ RLE    IPDVED  SG KG ELSRTPIEL + DW LK+VPWIGGRIISM+H PSG+QWLH R+EI+GYEEYSG EYRSAGC+EEY+++ R+L HAGEEESVLLEGDIGGGLVLQRQI  PK+   +++I SS++AR VGAGSGGFSR 
Sbjct:  619 SFLLGPVLVY------ASTTRNQGLDKLEVTLPKGIWLGFDFGDAHPDLPALYLKGGSIIPAGLPLQHVGEANPSDELTLLVALDESGKAEGFLFEDDGDGYEFTRGNYLLTHYSAQLQSTAVTVSVHRTEGSWKRPKRRLHIQLLLGGGAMLDTWGVDGEVLHVNLPSEEEVSKLVSTSEKQYKERLEKAIQIPDVEDEVSGPKGMELSRTPIELKSSDWLLKVVPWIGGRIISMIHFPSGTQWLHGRIEISGYEEYSGTEYRSAGCSEEYSIINRELGHAGEEESVLLEGDIGGGLVLQRQICFPKNAANIIQINSSIIARNVGAGSGGFSRF 947          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: G7K6R7_MEDTR (Neutral alpha-glucosidase OS=Medicago truncatula GN=MTR_5g026610 PE=3 SV=1)

HSP 1 Score: 956.436 bits (2471), Expect = 0.000e+0
Identity = 462/598 (77.26%), Postives = 519/598 (86.79%), Query Frame = 1

Query:   52 RFNPSPPSSTSKFLFRKKSYSKKLTGRRLISKMTDSE--VKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVM-SNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYS 1836
            R + + P  +S    R+K + +KL     ISKM + E  V + ++D   GKMIF+PIL DGVFRFDCS NDR+A YPS+SF+N+K R+ P+  ++K PSY P+FEC L QQ+V+LE P GTS YGTGEVSGQLERTGKRVFTWNTDAWGYGP T+SLYQSHPWVLAVLPNGEALGILADTTRRCEIDLR E  I+FI+  S+PVITFGPF+SPT VL+SLS AIGTVFMPPKWSLGY QCRWSY S +RVLE+++ FREK IPCDVIWMDIDYMDGFRCFTFDKERF DPKSLV+ LH  GFK IWMLDPGIKQE+GYFVYDSGS++DVW+QKADG  FVGDVWPGPCVFPD+TQSK RAWWANLVKDF+SNGVDGIWNDMNEPA+FK VTKTMPESN+HRGD ELGGCQ+H+ YHNVYG+LMARSTYEGM LA+  +RPFVLTRAGF GSQRYAATWTGDNLS WEHL MSISM+LQLGLSGQPLSGPDIGGFAGNATP+LFGRW+G G++FPFCRGHSE GT DHEPWSFG+ECEEVCRLALKRRYRLIP IYTLFY AHT+G PVATP FFADP DP+LR LE+ FLLGP+LVY+
Sbjct:   37 RLHRNTPFLSSIITLRRKRFIEKL-----ISKMANYEGQVSSGSTDVRTGKMIFEPILNDGVFRFDCSLNDRDAAYPSISFVNSKDRETPITGTHKVPSYTPTFECLLEQQVVQLELPVGTSLYGTGEVSGQLERTGKRVFTWNTDAWGYGPGTSSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRKESTIRFIAPSSYPVITFGPFASPTEVLISLSKAIGTVFMPPKWSLGYQQCRWSYLSDQRVLEVAKTFREKSIPCDVIWMDIDYMDGFRCFTFDKERFRDPKSLVESLHYSGFKGIWMLDPGIKQEKGYFVYDSGSENDVWVQKADGTAFVGDVWPGPCVFPDYTQSKVRAWWANLVKDFVSNGVDGIWNDMNEPAVFKAVTKTMPESNVHRGDGELGGCQNHSFYHNVYGLLMARSTYEGMKLANENRRPFVLTRAGFSGSQRYAATWTGDNLSTWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGSLFPFCRGHSEAGTTDHEPWSFGEECEEVCRLALKRRYRLIPLIYTLFYFAHTKGIPVATPTFFADPTDPSLRKLENSFLLGPVLVYA 629          

HSP 2 Score: 607.06 bits (1564), Expect = 0.000e+0
Identity = 300/445 (67.42%), Postives = 361/445 (81.12%), Query Frame = 2

Query: 2567 SFFILPCFTY*QFIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDG-SGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRLVCLRVHPTFSLLHPTETFVAFTSIDGTKQEVWPETGEKVYQGNLLPNGEWKLVDKCLGLVLINRFNVGEVYKCLIHWGARTVNLELWSEDRPVSKQSPLQIFHEYEVREI 3898
            SF + P   Y       S   + G DKL+  LPKGIWL FDF D+HPDLPALYL+GG IIP G P QHVGE+NP D+LTL+VALDE GKA+G LFEDDGDGY FT G YLLTHY A+L+ + VTV V +TEG WKRP RRLH+QLL+G  AMLD WG DGE L + +PSE EVSKL+S+S++ ++ RLE    IPDVED  SG KG ELSRTPIEL + DW LK+VPWIGGRIISM+H PSG+QWLH R+EI+GYEEYSG EYRSAGC+EEY+++ R+L HAGEEESVLLEGDIGGGLVLQRQI  PK+   +++I SS++AR VGAGSGGFSRLVCLR+HPTF+LLHP+E+FV+FTSI+G+  EV+P+ GE++++G+L+P+GEWKLVDKCLGL L+NRFNV EV KCL+HW   TVNLELWSE RPVS+QSP+QI H+YEV  I
Sbjct:  619 SFLLGPVLVY------ASTTRNQGLDKLEVTLPKGIWLGFDFGDAHPDLPALYLKGGSIIPAGLPLQHVGEANPSDELTLLVALDESGKAEGFLFEDDGDGYEFTRGNYLLTHYSAQLQSTAVTVSVHRTEGSWKRPKRRLHIQLLLGGGAMLDTWGVDGEVLHVNLPSEEEVSKLVSTSEKQYKERLEKAIQIPDVEDEVSGPKGMELSRTPIELKSSDWLLKVVPWIGGRIISMIHFPSGTQWLHGRIEISGYEEYSGTEYRSAGCSEEYSIINRELGHAGEEESVLLEGDIGGGLVLQRQICFPKNAANIIQINSSIIARNVGAGSGGFSRLVCLRIHPTFNLLHPSESFVSFTSINGSMHEVFPDGGEQIFEGHLIPDGEWKLVDKCLGLALVNRFNVTEVSKCLVHWDFGTVNLELWSESRPVSEQSPIQISHQYEVIRI 1057          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: U5GUB5_POPTR (Glycosyl hydrolase family 31 family protein OS=Populus trichocarpa GN=POPTR_0001s01170g PE=3 SV=1)

HSP 1 Score: 952.97 bits (2462), Expect = 0.000e+0
Identity = 471/612 (76.96%), Postives = 518/612 (84.64%), Query Frame = 1

Query:   37 PLAFSRFNPSPPSSTSKFLF--RKKSYSKKLTGRRLISKMTD-SEVKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVMSNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAI---------GTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYS 1836
            P   S F  S  + TS      RK+  +KKL+ R L+SKM D  + K   +D  +G MIFQPILEDG+FRFDCSA  R A YPSLSFI +  RD P+MS+  PSY P++EC  G+QIVK EFP GT+FYGTGEVSGQLERTGKRVFTWNTDAWGYGP TTSLYQSHPWVLAVLPNGEALG+LADTT RCEIDLR E  IQFI+  S+PV+TFG F+SPT VL SLSHAI         GTVFMPPKWSLGY QCRWSYDS ERV EI+R FREKGIPCDVIWMDIDYMDGFRCFTFD+     P+SLVKDLH+ GFKAIWMLDPGIK+EEGY +YDSGS++D WI+KADG+PFVG+VWPGPCVFPDFTQSK RAWWA LVKDF SNGVDGIWNDMNEPA+FKTVTKTMPESN+H GD E+GGCQ+H+HYHNVYGMLMARSTYEG+ LA+  KRPFVLTRAGFIGSQRYAATWTGDNLSNWEH+ MSISM+LQLGLSGQPLSGPDIGGFAGNATPKLFGRW+G GAMFPFCRGHSE  T DHEPWSFG+ECEEVCRLALKRRYRL+PHIYTLFY+AHT G PVATP FFADPKDP LRT E+ FLLGPLLV+S
Sbjct:   29 PPPHSLFPTSTTTCTSAVTLASRKRRLNKKLSCRGLMSKMADHDQAKVVAADVVSGDMIFQPILEDGIFRFDCSAEARAASYPSLSFIRSSDRDTPIMSHSVPSYTPTYECVSGKQIVKFEFPDGTTFYGTGEVSGQLERTGKRVFTWNTDAWGYGPGTTSLYQSHPWVLAVLPNGEALGVLADTTLRCEIDLRKESIIQFIAPSSYPVVTFGLFASPTDVLKSLSHAIVVYAVSLSSGTVFMPPKWSLGYQQCRWSYDSDERVREIARTFREKGIPCDVIWMDIDYMDGFRCFTFDQAY---PQSLVKDLHDDGFKAIWMLDPGIKKEEGYLIYDSGSENDAWIKKADGEPFVGEVWPGPCVFPDFTQSKVRAWWALLVKDFTSNGVDGIWNDMNEPAVFKTVTKTMPESNLHLGDEEIGGCQNHSHYHNVYGMLMARSTYEGIKLANENKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHVHMSISMVLQLGLSGQPLSGPDIGGFAGNATPKLFGRWMGVGAMFPFCRGHSEKSTNDHEPWSFGEECEEVCRLALKRRYRLLPHIYTLFYLAHTTGIPVATPTFFADPKDPGLRTTENSFLLGPLLVFS 637          

HSP 2 Score: 617.846 bits (1592), Expect = 0.000e+0
Identity = 306/430 (71.16%), Postives = 365/430 (84.88%), Query Frame = 2

Query: 2618 SIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRL--ESIKHIPDVEDGSGHKGG-ELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRLVCLRVHPTFSLLHPTETFVAFTSIDGTKQEVWPETGEKVYQGNLLPNGEWKLVDKCLGLVLINRFNVGEVYKCLIHWGARTVNLELWSEDRPVSKQSPLQIFHEYEVREI 3898
            S + D G D+L P+LPKGIWL FDFDDSHPDLP LYLQGG IIP  PPHQHVGE+N  DDLTL+VALD++G A+G LFED+GDGY FT G YLLT YVAEL+ S VTV+VS+ EG WKRP RRL VQLL+G  AMLD+WG DG+ L+I MP+E+EVS L+S+S++ +R RL  E  KHIP++E+ SG KG  +LS+ P+EL NGDW  K+VPWIGGRIISM HLPSG+QWLHSRVEI+GYEEYSG EYRSAGC+EEY+V++RDLEHA EEES++LEG+IGGGLVL+RQI++ KDNPK+L+I+S ++AR VGAGSGGFSRLVCLRVHP F+LLHPTETFV+FTSIDG+K E+WPE+G++ YQ NLLPNGEW LVD+C GL L+NRFN+ EV+KC IHWG  TVNLELWSEDRPVSKQSPL + H YEVR I
Sbjct:  638 STIADQGMDRLHPVLPKGIWLRFDFDDSHPDLPTLYLQGGSIIPLAPPHQHVGEANLSDDLTLLVALDQNGHAEGLLFEDEGDGYEFTRGGYLLTRYVAELQSSAVTVRVSQMEGSWKRPRRRLRVQLLLGGGAMLDSWGIDGDVLKINMPTEVEVSTLVSTSEKQYRTRLGIECAKHIPELEEVSGPKGVVDLSKVPVELKNGDWIAKVVPWIGGRIISMEHLPSGTQWLHSRVEIDGYEEYSGTEYRSAGCSEEYSVIERDLEHAEEEESLILEGNIGGGLVLRRQISILKDNPKILQIDSGIIARSVGAGSGGFSRLVCLRVHPAFTLLHPTETFVSFTSIDGSKHEIWPESGDQFYQENLLPNGEWMLVDQCQGLALVNRFNINEVFKCYIHWGTGTVNLELWSEDRPVSKQSPLTVSHGYEVRGI 1067          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: A0A059C324_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_E01212 PE=3 SV=1)

HSP 1 Score: 951.429 bits (2458), Expect = 0.000e+0
Identity = 458/568 (80.63%), Postives = 500/568 (88.03%), Query Frame = 1

Query:  127 GRRLISKMTDSEVKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVMSNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLV 1830
            G RL +KMT  E +A  +D TAG MIF+PILE+GVFRFDCSANDR A +PSLSF+N K RD P+  +  PSYIP+++C  GQQIVKLE PAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGP TTSLYQSHPWVLAVLP+GE+LGILADTTRRCEIDLR +  IQF +  S+PVITFGPF+SP AVL SLSHA GTVFMPPKWSLGY QCRWSYDS  RV EI+  FREK IPCDV+WMDIDYMDGFRCFTFDK+RF DPKSLV  LH  GFKAIWMLDPGIK+EEGYFV+DSG K DVW++ ADG PFVG+VWPGPCVFPDFTQSK R WWA LV+DFISNGVDGIWNDMNEPA+FK+VTKTMPESNIHRGD+ELGG Q+H+HYHNVYGMLMARSTYEG+  ++  KRPFVLTRAGF+GSQRYAATWTGDNLSNWEHL MSISM+LQLGLSGQPLSGPDIGGF GNATPKLFGRW+G GAMFPFCRGHSE  T DHEPWSFG+ECEEVCRLALKRRYR +PHIYTLFYMAH  GT VA+P FFADPKDP LRTLE+ FLLGPLLV
Sbjct:   81 GSRLKTKMTIREGQAVAADVTAGDMIFEPILEEGVFRFDCSANDRAAAFPSLSFVNGKDRDTPITIHHVPSYIPTYQCLFGQQIVKLELPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPGTTSLYQSHPWVLAVLPSGESLGILADTTRRCEIDLRNKSIIQFTAPSSYPVITFGPFASPGAVLTSLSHASGTVFMPPKWSLGYQQCRWSYDSDNRVREITSTFREKEIPCDVVWMDIDYMDGFRCFTFDKDRFSDPKSLVDSLHRDGFKAIWMLDPGIKREEGYFVFDSGCKSDVWMKAADGTPFVGEVWPGPCVFPDFTQSKVRHWWAQLVQDFISNGVDGIWNDMNEPAVFKSVTKTMPESNIHRGDDELGGVQNHSHYHNVYGMLMARSTYEGIKSSNENKRPFVLTRAGFVGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFVGNATPKLFGRWMGIGAMFPFCRGHSEMDTNDHEPWSFGEECEEVCRLALKRRYRFLPHIYTLFYMAHKTGTLVASPTFFADPKDPRLRTLENSFLLGPLLV 648          

HSP 2 Score: 464.151 bits (1193), Expect = 5.832e-139
Identity = 221/321 (68.85%), Postives = 269/321 (83.80%), Query Frame = 2

Query: 2603 FIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDGSGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSR 3565
             +   S MP   S+ LQ +LPKGIWL+FDFDD+HPDLP L+LQGG IIP GPPHQHVGE+N  DDLTL+VAL+EHG AKG L+EDDGDGY + EGEYLLTHY+AE E S+VT++V   EG WKRP  RLHV LL+G  AM+DAWG DG+ +QI +PSE EVSK++++S++ +  RLES K IPDVE+ S HKG EL+RTPIEL +G+W  K+VPWIGGRIISM H+PSG+QWLHSR+EINGYEEYSG EYRSAGCTE+Y V++R+LEHAGEEES+++EG+IGGGL +QR I++ KDNPKV RI+SSL+ARKVGAGSGGFSR
Sbjct:  646 LLVCASSMPHQESNDLQHVLPKGIWLTFDFDDAHPDLPLLFLQGGSIIPLGPPHQHVGEANLSDDLTLLVALNEHGTAKGILYEDDGDGYKYLEGEYLLTHYIAERESSLVTIRVYTEEGSWKRPKCRLHVMLLLGGGAMVDAWGMDGDTIQITLPSEDEVSKMVAASEKKYNARLESCKRIPDVEEISSHKGVELARTPIELKSGEWIAKVVPWIGGRIISMEHVPSGTQWLHSRIEINGYEEYSGREYRSAGCTEDYTVIERNLEHAGEEESLMMEGEIGGGLAIQRLISIRKDNPKVFRIDSSLVARKVGAGSGGFSR 966          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: A0A072UDZ0_MEDTR (Neutral alpha-glucosidase OS=Medicago truncatula GN=MTR_5g026610 PE=3 SV=1)

HSP 1 Score: 951.429 bits (2458), Expect = 0.000e+0
Identity = 456/572 (79.72%), Postives = 508/572 (88.81%), Query Frame = 1

Query:  130 RRLISKMTDSE--VKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVM-SNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYS 1836
             +LISKM + E  V + ++D   GKMIF+PIL DGVFRFDCS NDR+A YPS+SF+N+K R+ P+  ++K PSY P+FEC L QQ+V+LE P GTS YGTGEVSGQLERTGKRVFTWNTDAWGYGP T+SLYQSHPWVLAVLPNGEALGILADTTRRCEIDLR E  I+FI+  S+PVITFGPF+SPT VL+SLS AIGTVFMPPKWSLGY QCRWSY S +RVLE+++ FREK IPCDVIWMDIDYMDGFRCFTFDKERF DPKSLV+ LH  GFK IWMLDPGIKQE+GYFVYDSGS++DVW+QKADG  FVGDVWPGPCVFPD+TQSK RAWWANLVKDF+SNGVDGIWNDMNEPA+FK VTKTMPESN+HRGD ELGGCQ+H+ YHNVYG+LMARSTYEGM LA+  +RPFVLTRAGF GSQRYAATWTGDNLS WEHL MSISM+LQLGLSGQPLSGPDIGGFAGNATP+LFGRW+G G++FPFCRGHSE GT DHEPWSFG+ECEEVCRLALKRRYRLIP IYTLFY AHT+G PVATP FFADP DP+LR LE+ FLLGP+LVY+
Sbjct:   98 EKLISKMANYEGQVSSGSTDVRTGKMIFEPILNDGVFRFDCSLNDRDAAYPSISFVNSKDRETPITGTHKVPSYTPTFECLLEQQVVQLELPVGTSLYGTGEVSGQLERTGKRVFTWNTDAWGYGPGTSSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRKESTIRFIAPSSYPVITFGPFASPTEVLISLSKAIGTVFMPPKWSLGYQQCRWSYLSDQRVLEVAKTFREKSIPCDVIWMDIDYMDGFRCFTFDKERFRDPKSLVESLHYSGFKGIWMLDPGIKQEKGYFVYDSGSENDVWVQKADGTAFVGDVWPGPCVFPDYTQSKVRAWWANLVKDFVSNGVDGIWNDMNEPAVFKAVTKTMPESNVHRGDGELGGCQNHSFYHNVYGLLMARSTYEGMKLANENRRPFVLTRAGFSGSQRYAATWTGDNLSTWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGSLFPFCRGHSEAGTTDHEPWSFGEECEEVCRLALKRRYRLIPLIYTLFYFAHTKGIPVATPTFFADPTDPSLRKLENSFLLGPVLVYA 669          

HSP 2 Score: 607.06 bits (1564), Expect = 0.000e+0
Identity = 300/445 (67.42%), Postives = 361/445 (81.12%), Query Frame = 2

Query: 2567 SFFILPCFTY*QFIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDG-SGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRLVCLRVHPTFSLLHPTETFVAFTSIDGTKQEVWPETGEKVYQGNLLPNGEWKLVDKCLGLVLINRFNVGEVYKCLIHWGARTVNLELWSEDRPVSKQSPLQIFHEYEVREI 3898
            SF + P   Y       S   + G DKL+  LPKGIWL FDF D+HPDLPALYL+GG IIP G P QHVGE+NP D+LTL+VALDE GKA+G LFEDDGDGY FT G YLLTHY A+L+ + VTV V +TEG WKRP RRLH+QLL+G  AMLD WG DGE L + +PSE EVSKL+S+S++ ++ RLE    IPDVED  SG KG ELSRTPIEL + DW LK+VPWIGGRIISM+H PSG+QWLH R+EI+GYEEYSG EYRSAGC+EEY+++ R+L HAGEEESVLLEGDIGGGLVLQRQI  PK+   +++I SS++AR VGAGSGGFSRLVCLR+HPTF+LLHP+E+FV+FTSI+G+  EV+P+ GE++++G+L+P+GEWKLVDKCLGL L+NRFNV EV KCL+HW   TVNLELWSE RPVS+QSP+QI H+YEV  I
Sbjct:  659 SFLLGPVLVY------ASTTRNQGLDKLEVTLPKGIWLGFDFGDAHPDLPALYLKGGSIIPAGLPLQHVGEANPSDELTLLVALDESGKAEGFLFEDDGDGYEFTRGNYLLTHYSAQLQSTAVTVSVHRTEGSWKRPKRRLHIQLLLGGGAMLDTWGVDGEVLHVNLPSEEEVSKLVSTSEKQYKERLEKAIQIPDVEDEVSGPKGMELSRTPIELKSSDWLLKVVPWIGGRIISMIHFPSGTQWLHGRIEISGYEEYSGTEYRSAGCSEEYSIINRELGHAGEEESVLLEGDIGGGLVLQRQICFPKNAANIIQINSSIIARNVGAGSGGFSRLVCLRIHPTFNLLHPSESFVSFTSINGSMHEVFPDGGEQIFEGHLIPDGEWKLVDKCLGLALVNRFNVTEVSKCLVHWDFGTVNLELWSESRPVSEQSPIQISHQYEVIRI 1097          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: A0A059C3H7_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_E01212 PE=3 SV=1)

HSP 1 Score: 950.273 bits (2455), Expect = 0.000e+0
Identity = 458/568 (80.63%), Postives = 500/568 (88.03%), Query Frame = 1

Query:  127 GRRLISKMTDSEVKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVMSNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLV 1830
            G RL +KMT  E +A  +D TAG MIF+PILE+GVFRFDCSANDR A +PSLSF+N K RD P+  +  PSYIP+++C  GQQIVKLE PAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGP TTSLYQSHPWVLAVLP+GE+LGILADTTRRCEIDLR +  IQF +  S+PVITFGPF+SP AVL SLSHA GTVFMPPKWSLGY QCRWSYDS  RV EI+  FREK IPCDV+WMDIDYMDGFRCFTFDK+RF DPKSLV  LH  GFKAIWMLDPGIK+EEGYFV+DSG K DVW++ ADG PFVG+VWPGPCVFPDFTQSK R WWA LV+DFISNGVDGIWNDMNEPA+FK+VTKTMPESNIHRGD+ELGG Q+H+HYHNVYGMLMARSTYEG+  ++  KRPFVLTRAGF+GSQRYAATWTGDNLSNWEHL MSISM+LQLGLSGQPLSGPDIGGF GNATPKLFGRW+G GAMFPFCRGHSE  T DHEPWSFG+ECEEVCRLALKRRYR +PHIYTLFYMAH  GT VA+P FFADPKDP LRTLE+ FLLGPLLV
Sbjct:   81 GSRLKTKMTIREGQAVAADVTAGDMIFEPILEEGVFRFDCSANDRAAAFPSLSFVNGKDRDTPITIHHVPSYIPTYQCLFGQQIVKLELPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPGTTSLYQSHPWVLAVLPSGESLGILADTTRRCEIDLRNKSIIQFTAPSSYPVITFGPFASPGAVLTSLSHASGTVFMPPKWSLGYQQCRWSYDSDNRVREITSTFREKEIPCDVVWMDIDYMDGFRCFTFDKDRFSDPKSLVDSLHRDGFKAIWMLDPGIKREEGYFVFDSGCKSDVWMKAADGTPFVGEVWPGPCVFPDFTQSKVRHWWAQLVQDFISNGVDGIWNDMNEPAVFKSVTKTMPESNIHRGDDELGGVQNHSHYHNVYGMLMARSTYEGIKSSNENKRPFVLTRAGFVGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFVGNATPKLFGRWMGIGAMFPFCRGHSEMDTNDHEPWSFGEECEEVCRLALKRRYRFLPHIYTLFYMAHKTGTLVASPTFFADPKDPRLRTLENSFLLGPLLV 648          

HSP 2 Score: 645.195 bits (1663), Expect = 0.000e+0
Identity = 300/429 (69.93%), Postives = 368/429 (85.78%), Query Frame = 2

Query: 2603 FIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDGSGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRLVCLRVHPTFSLLHPTETFVAFTSIDGTKQEVWPETGEKVYQGNLLPNGEWKLVDKCLGLVLINRFNVGEVYKCLIHWGARTVNLELWSEDRPVSKQSPLQIFHEYEV 3889
             +   S MP   S+ LQ +LPKGIWL+FDFDD+HPDLP L+LQGG IIP GPPHQHVGE+N  DDLTL+VAL+EHG AKG L+EDDGDGY + EGEYLLTHY+AE E S+VT++V   EG WKRP  RLHV LL+G  AM+DAWG DG+ +QI +PSE EVSK++++S++ +  RLES K IPDVE+ S HKG EL+RTPIEL +G+W  K+VPWIGGRIISM H+PSG+QWLHSR+EINGYEEYSG EYRSAGCTE+Y V++R+LEHAGEEES+++EG+IGGGL +QR I++ KDNPKV RI+SSL+ARKVGAGSGGFSRLVCLRVHPTF+LLHPTE+F++FT++DG+K E+WP++GE+ ++GNLLPNGEW L DKCLG+ L+NRFNV EV+KCLIHWG  TVNLELWSE+RPVSK+SPL++ HEYEV
Sbjct:  646 LLVCASSMPHQESNDLQHVLPKGIWLTFDFDDAHPDLPLLFLQGGSIIPLGPPHQHVGEANLSDDLTLLVALNEHGTAKGILYEDDGDGYKYLEGEYLLTHYIAERESSLVTIRVYTEEGSWKRPKCRLHVMLLLGGGAMVDAWGMDGDTIQITLPSEDEVSKMVAASEKKYNARLESCKRIPDVEEISSHKGVELARTPIELKSGEWIAKVVPWIGGRIISMEHVPSGTQWLHSRIEINGYEEYSGREYRSAGCTEDYTVIERNLEHAGEEESLMMEGEIGGGLAIQRLISIRKDNPKVFRIDSSLVARKVGAGSGGFSRLVCLRVHPTFTLLHPTESFISFTAVDGSKHELWPDSGEQTFEGNLLPNGEWSLWDKCLGVGLVNRFNVNEVFKCLIHWGTGTVNLELWSEERPVSKESPLKVSHEYEV 1074          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: A0A072UP95_MEDTR (Neutral alpha-glucosidase OS=Medicago truncatula GN=MTR_5g026610 PE=3 SV=1)

HSP 1 Score: 947.962 bits (2449), Expect = 0.000e+0
Identity = 450/562 (80.07%), Postives = 502/562 (89.32%), Query Frame = 1

Query:  154 DSEVKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVM-SNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYS 1836
            + +V + ++D   GKMIF+PIL DGVFRFDCS NDR+A YPS+SF+N+K R+ P+  ++K PSY P+FEC L QQ+V+LE P GTS YGTGEVSGQLERTGKRVFTWNTDAWGYGP T+SLYQSHPWVLAVLPNGEALGILADTTRRCEIDLR E  I+FI+  S+PVITFGPF+SPT VL+SLS AIGTVFMPPKWSLGY QCRWSY S +RVLE+++ FREK IPCDVIWMDIDYMDGFRCFTFDKERF DPKSLV+ LH  GFK IWMLDPGIKQE+GYFVYDSGS++DVW+QKADG  FVGDVWPGPCVFPD+TQSK RAWWANLVKDF+SNGVDGIWNDMNEPA+FK VTKTMPESN+HRGD ELGGCQ+H+ YHNVYG+LMARSTYEGM LA+  +RPFVLTRAGF GSQRYAATWTGDNLS WEHL MSISM+LQLGLSGQPLSGPDIGGFAGNATP+LFGRW+G G++FPFCRGHSE GT DHEPWSFG+ECEEVCRLALKRRYRLIP IYTLFY AHT+G PVATP FFADP DP+LR LE+ FLLGP+LVY+
Sbjct:    5 EGQVSSGSTDVRTGKMIFEPILNDGVFRFDCSLNDRDAAYPSISFVNSKDRETPITGTHKVPSYTPTFECLLEQQVVQLELPVGTSLYGTGEVSGQLERTGKRVFTWNTDAWGYGPGTSSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRKESTIRFIAPSSYPVITFGPFASPTEVLISLSKAIGTVFMPPKWSLGYQQCRWSYLSDQRVLEVAKTFREKSIPCDVIWMDIDYMDGFRCFTFDKERFRDPKSLVESLHYSGFKGIWMLDPGIKQEKGYFVYDSGSENDVWVQKADGTAFVGDVWPGPCVFPDYTQSKVRAWWANLVKDFVSNGVDGIWNDMNEPAVFKAVTKTMPESNVHRGDGELGGCQNHSFYHNVYGLLMARSTYEGMKLANENRRPFVLTRAGFSGSQRYAATWTGDNLSTWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGSLFPFCRGHSEAGTTDHEPWSFGEECEEVCRLALKRRYRLIPLIYTLFYFAHTKGIPVATPTFFADPTDPSLRKLENSFLLGPVLVYA 566          

HSP 2 Score: 437.187 bits (1123), Expect = 3.741e-130
Identity = 223/335 (66.57%), Postives = 263/335 (78.51%), Query Frame = 2

Query: 2567 SFFILPCFTY*QFIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDG-SGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRL 3568
            SF + P   Y       S   + G DKL+  LPKGIWL FDF D+HPDLPALYL+GG IIP G P QHVGE+NP D+LTL+VALDE GKA+G LFEDDGDGY FT G YLLTHY A+L+ + VTV V +TEG WKRP RRLH+QLL+G  AMLD WG DGE L + +PSE EVSKL+S+S++ ++ RLE    IPDVED  SG KG ELSRTPIEL + DW LK+VPWIGGRIISM+H PSG+QWLH R+EI+GYEEYSG EYRSAGC+EEY+++ R+L HAGEEESVLLEGDIGGGLVLQRQI  PK+   +++I SS++AR VGAGSGGFSR 
Sbjct:  556 SFLLGPVLVY------ASTTRNQGLDKLEVTLPKGIWLGFDFGDAHPDLPALYLKGGSIIPAGLPLQHVGEANPSDELTLLVALDESGKAEGFLFEDDGDGYEFTRGNYLLTHYSAQLQSTAVTVSVHRTEGSWKRPKRRLHIQLLLGGGAMLDTWGVDGEVLHVNLPSEEEVSKLVSTSEKQYKERLEKAIQIPDVEDEVSGPKGMELSRTPIELKSSDWLLKVVPWIGGRIISMIHFPSGTQWLHGRIEISGYEEYSGTEYRSAGCSEEYSIINRELGHAGEEESVLLEGDIGGGLVLQRQICFPKNAANIIQINSSIIARNVGAGSGGFSRF 884          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: A0A072UEX7_MEDTR (Neutral alpha-glucosidase OS=Medicago truncatula GN=MTR_5g026610 PE=3 SV=1)

HSP 1 Score: 946.421 bits (2445), Expect = 0.000e+0
Identity = 450/562 (80.07%), Postives = 502/562 (89.32%), Query Frame = 1

Query:  154 DSEVKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVM-SNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYS 1836
            + +V + ++D   GKMIF+PIL DGVFRFDCS NDR+A YPS+SF+N+K R+ P+  ++K PSY P+FEC L QQ+V+LE P GTS YGTGEVSGQLERTGKRVFTWNTDAWGYGP T+SLYQSHPWVLAVLPNGEALGILADTTRRCEIDLR E  I+FI+  S+PVITFGPF+SPT VL+SLS AIGTVFMPPKWSLGY QCRWSY S +RVLE+++ FREK IPCDVIWMDIDYMDGFRCFTFDKERF DPKSLV+ LH  GFK IWMLDPGIKQE+GYFVYDSGS++DVW+QKADG  FVGDVWPGPCVFPD+TQSK RAWWANLVKDF+SNGVDGIWNDMNEPA+FK VTKTMPESN+HRGD ELGGCQ+H+ YHNVYG+LMARSTYEGM LA+  +RPFVLTRAGF GSQRYAATWTGDNLS WEHL MSISM+LQLGLSGQPLSGPDIGGFAGNATP+LFGRW+G G++FPFCRGHSE GT DHEPWSFG+ECEEVCRLALKRRYRLIP IYTLFY AHT+G PVATP FFADP DP+LR LE+ FLLGP+LVY+
Sbjct:    5 EGQVSSGSTDVRTGKMIFEPILNDGVFRFDCSLNDRDAAYPSISFVNSKDRETPITGTHKVPSYTPTFECLLEQQVVQLELPVGTSLYGTGEVSGQLERTGKRVFTWNTDAWGYGPGTSSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRKESTIRFIAPSSYPVITFGPFASPTEVLISLSKAIGTVFMPPKWSLGYQQCRWSYLSDQRVLEVAKTFREKSIPCDVIWMDIDYMDGFRCFTFDKERFRDPKSLVESLHYSGFKGIWMLDPGIKQEKGYFVYDSGSENDVWVQKADGTAFVGDVWPGPCVFPDYTQSKVRAWWANLVKDFVSNGVDGIWNDMNEPAVFKAVTKTMPESNVHRGDGELGGCQNHSFYHNVYGLLMARSTYEGMKLANENRRPFVLTRAGFSGSQRYAATWTGDNLSTWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGSLFPFCRGHSEAGTTDHEPWSFGEECEEVCRLALKRRYRLIPLIYTLFYFAHTKGIPVATPTFFADPTDPSLRKLENSFLLGPVLVYA 566          

HSP 2 Score: 606.29 bits (1562), Expect = 0.000e+0
Identity = 300/445 (67.42%), Postives = 361/445 (81.12%), Query Frame = 2

Query: 2567 SFFILPCFTY*QFIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDG-SGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRLVCLRVHPTFSLLHPTETFVAFTSIDGTKQEVWPETGEKVYQGNLLPNGEWKLVDKCLGLVLINRFNVGEVYKCLIHWGARTVNLELWSEDRPVSKQSPLQIFHEYEVREI 3898
            SF + P   Y       S   + G DKL+  LPKGIWL FDF D+HPDLPALYL+GG IIP G P QHVGE+NP D+LTL+VALDE GKA+G LFEDDGDGY FT G YLLTHY A+L+ + VTV V +TEG WKRP RRLH+QLL+G  AMLD WG DGE L + +PSE EVSKL+S+S++ ++ RLE    IPDVED  SG KG ELSRTPIEL + DW LK+VPWIGGRIISM+H PSG+QWLH R+EI+GYEEYSG EYRSAGC+EEY+++ R+L HAGEEESVLLEGDIGGGLVLQRQI  PK+   +++I SS++AR VGAGSGGFSRLVCLR+HPTF+LLHP+E+FV+FTSI+G+  EV+P+ GE++++G+L+P+GEWKLVDKCLGL L+NRFNV EV KCL+HW   TVNLELWSE RPVS+QSP+QI H+YEV  I
Sbjct:  556 SFLLGPVLVY------ASTTRNQGLDKLEVTLPKGIWLGFDFGDAHPDLPALYLKGGSIIPAGLPLQHVGEANPSDELTLLVALDESGKAEGFLFEDDGDGYEFTRGNYLLTHYSAQLQSTAVTVSVHRTEGSWKRPKRRLHIQLLLGGGAMLDTWGVDGEVLHVNLPSEEEVSKLVSTSEKQYKERLEKAIQIPDVEDEVSGPKGMELSRTPIELKSSDWLLKVVPWIGGRIISMIHFPSGTQWLHGRIEISGYEEYSGTEYRSAGCSEEYSIINRELGHAGEEESVLLEGDIGGGLVLQRQICFPKNAANIIQINSSIIARNVGAGSGGFSRLVCLRIHPTFNLLHPSESFVSFTSINGSMHEVFPDGGEQIFEGHLIPDGEWKLVDKCLGLALVNRFNVTEVSKCLVHWDFGTVNLELWSESRPVSEQSPIQISHQYEVIRI 994          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: K4CMS0_SOLLC (Uncharacterized protein OS=Solanum lycopersicum GN=Solyc08g075530.2 PE=3 SV=1)

HSP 1 Score: 944.495 bits (2440), Expect = 0.000e+0
Identity = 452/584 (77.40%), Postives = 509/584 (87.16%), Query Frame = 1

Query:   91 LFRKKSYSKKLTGRRLIS-KMTDSEVKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVMS-NKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYS 1836
            L R +S +K   G   +  KM   E   A SD+  G MIF+ ILE+GVFRFDCSA+DR A +PS+SF++ K R+ P+MS +K PSYIP+FEC  GQQIV +E P+GTSFYGTGEVSGQLERTGKR+ TWNTDAWGYGP TTSLYQSHPWVLAVLP+GE LG+LADTT RCE+DLR E  I+FIS  SFPVITFGPF SP  VLVSLSHAIGTVFMPPKWSLGYHQCRWSY    RV EI+R FREK IPCDVIWMDIDYM+GFRCFTFDKERFPDP+SLV++LH  GFKAIWMLDPGIK E+GYF YDSGS+ DVW+Q ADG+P+VGDVWPGPCVFPDFTQ KAR+WWANLVKDFISNGVDGIWNDMNEPA+FKTVTKTMPE+NIHRGD E GGCQ+H++YHNVYGMLMARSTYEGM LA+  KRPFVLTRAGF+GSQRYAATWTGDNLS WEHLQMSI M+LQLGLSGQPL+GPDIGGFAGNATP++FGRW+G G++FPFCR HSE  T DHEPWSFG+ECEEVCRLAL+RRYRL+PHIYTLFY+AHTRGTPV+ P+FFADPKDP LR LE+ FLLGP+L+Y+
Sbjct:   58 LIRGRSVNKGFIGASFVMLKMGGIEGTTAMSDARTGNMIFESILEEGVFRFDCSADDRNAAFPSISFVDPKVRETPLMSIHKVPSYIPTFECVRGQQIVNIELPSGTSFYGTGEVSGQLERTGKRILTWNTDAWGYGPGTTSLYQSHPWVLAVLPSGETLGVLADTTHRCEVDLRQESNIRFISRQSFPVITFGPFPSPIDVLVSLSHAIGTVFMPPKWSLGYHQCRWSYVPDTRVREIARTFREKKIPCDVIWMDIDYMNGFRCFTFDKERFPDPESLVEELHKSGFKAIWMLDPGIKNEKGYFAYDSGSEADVWVQTADGRPYVGDVWPGPCVFPDFTQLKARSWWANLVKDFISNGVDGIWNDMNEPAVFKTVTKTMPENNIHRGDPEFGGCQNHSYYHNVYGMLMARSTYEGMKLANGNKRPFVLTRAGFVGSQRYAATWTGDNLSTWEHLQMSIPMVLQLGLSGQPLTGPDIGGFAGNATPRMFGRWMGVGSLFPFCRAHSEADTNDHEPWSFGEECEEVCRLALERRYRLLPHIYTLFYLAHTRGTPVSAPIFFADPKDPELRKLENSFLLGPILIYA 641          

HSP 2 Score: 625.55 bits (1612), Expect = 0.000e+0
Identity = 299/444 (67.34%), Postives = 367/444 (82.66%), Query Frame = 2

Query: 2567 SFFILPCFTY*QFIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDGSGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRLVCLRVHPTFSLLHPTETFVAFTSIDGTKQEVWPETGEKVYQGNLLPNGEWKLVDKCLGLVLINRFNVGEVYKCLIHWGARTVNLELWSEDRPVSKQSPLQIFHEYEVREI 3898
            SF + P   Y       S   D   D     LP+GIWLSFDFDDSHPDLPALYL GG IIP GP +QHVG+++P DDLTL++ALDE+GKA+G LFEDDGDGY +++G YLLT YVAEL+ SVVTV+V+KTEG W+RP RRLHV++L+G+ AMLDAWG+DGE +Q+ MPSE +VS L+S S+E +R RLE  K IPDVE  SGHKG ELSRTP+ L +GDW LK VPWIGGRI+SM H+PSG+QWLHSRVEINGYEEYS  EYRSAGCTEEY+V++RDLE  GE ES+ LEGDIGGGL ++R I++PKDN KV RI+S ++AR VGAGSGGFSRLVCLRVHP F+LLHPTE++V+FTS++G+K E+WPE+GE+V++G+L P GEW LVD+CLGL L+NRFN+ +V+KC++HWG  TVNLELWSE+RPVSK SPL+I HEYEV++I
Sbjct:  631 SFLLGPILIY------ASTQRDEELDTAHHKLPRGIWLSFDFDDSHPDLPALYLLGGSIIPVGPLYQHVGQADPSDDLTLLIALDENGKAEGLLFEDDGDGYEYSQGGYLLTTYVAELQSSVVTVQVAKTEGNWRRPKRRLHVRILLGKGAMLDAWGSDGEIIQLAMPSETDVSNLVSESEEKYRNRLEGAKRIPDVETISGHKGVELSRTPVVLKSGDWELKAVPWIGGRILSMDHVPSGTQWLHSRVEINGYEEYSNREYRSAGCTEEYSVIERDLEQEGESESLRLEGDIGGGLFMERYISLPKDNSKVFRIDSGIVARGVGAGSGGFSRLVCLRVHPMFTLLHPTESYVSFTSLNGSKHELWPESGEQVFEGDLRPKGEWMLVDRCLGLGLVNRFNIDQVHKCMVHWGTGTVNLELWSEERPVSKDSPLKISHEYEVQKI 1068          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: D7L3E3_ARALL (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_898886 PE=3 SV=1)

HSP 1 Score: 924.85 bits (2389), Expect = 0.000e+0
Identity = 442/573 (77.14%), Postives = 500/573 (87.26%), Query Frame = 1

Query:  163 VKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVMSNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYSRFSISPFKDEFYRIV 1881
            V   +S++ +  MIF+PILE GVFRFD S + R+AV+PS+SF N+K R+VP++S+  P+YIP+  C   QQ+V  EF  GTSFYGTGEVSGQLERTGKRVFTWNTDAWGYG  TTSLYQSHPWVL VLP GE LG+LADTTR+CEIDLR E  I+ IS  S+P+ITFGPFSSPTAVL SLSHAIGTVFMPPKW+LGYHQCRWSY S +RV EI++ FR+K IP DVIWMDIDYMDGFRCFTFDKERFPDP +L KDLH+ GFKAIWMLDPGIKQEEGY+VYDSGSK+DVWI +ADGKPF+G+VWPGPCVFPD+T SKAR+WWANLVK+F+SNGVDGIWNDMNEPA+FK VTKTMPE+NIHRGD++LGG Q+H+HYHNVYGMLMARSTYEGM LAD+ KRPFVLTRAGFIGSQRYAATWTGDNLSNWEHL MSISM+LQLGLSGQPLSGPDIGGFAGNATP+LFGRW+G GAMFPFCRGHSE GT DHEPWSFG+ECEEVCR ALKRRY+L+PH YTLFY+AHT G PVA P+FFADPKD  LRT+E+ FLLGPLL+Y+    S    E   I+
Sbjct:    3 VSGDSSETVSTDMIFEPILEHGVFRFDSSVDHRKAVFPSVSFKNSKDREVPIVSHSVPAYIPTSVCLQDQQVVTFEFSPGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGSGTTSLYQSHPWVLVVLPTGETLGVLADTTRKCEIDLRKEGIIRIISPTSYPIITFGPFSSPTAVLESLSHAIGTVFMPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDIDYMDGFRCFTFDKERFPDPSALTKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWISRADGKPFIGEVWPGPCVFPDYTNSKARSWWANLVKEFVSNGVDGIWNDMNEPAVFKVVTKTMPENNIHRGDDDLGGVQNHSHYHNVYGMLMARSTYEGMELADKNKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGAMFPFCRGHSEAGTADHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYIAHTTGAPVAAPIFFADPKDSRLRTVENGFLLGPLLLYASTLSSQGSHELQHIL 575          

HSP 2 Score: 621.313 bits (1601), Expect = 0.000e+0
Identity = 294/429 (68.53%), Postives = 359/429 (83.68%), Query Frame = 2

Query: 2603 FIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDGSGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRLVCLRVHPTFSLLHPTETFVAFTSIDGTKQEVWPETGEKVYQGNLLPNGEWKLVDKCLGLVLINRFNVGEVYKCLIHWGARTVNLELWSEDRPVSKQSPLQIFHEYEV 3889
             +   S +   GS +LQ +LP+GIWL FDF+DSHPDLP LYLQGG II   PPH HVGE +  DDLTL+V+LDE+GKAKG LFEDDGDGYG+T+G +L+THY+AE   S VTVKVSKTEG W+RP RR+HVQLL+G  AMLDAWG DGE + I++PSE EVS+LIS+S E  +L +E+ K IP+ E   G KG ELS+ P+EL +GDW L IVPWIGGRI+SM H+PSG QWLHSR++INGYEEYSG EYRSAGCTEEYNV++RDLEHAGEEES++LEGD+GGGLVL+R+I++PK+NP+V +I SS+ AR VGAGSGGFSRLVCLRVHPTF+LLHPTE+FV+F SIDG+K EVWP++ E++Y+GN LP+GEW LVDK L L L+N+FNV +V+KC++HW   TVNLELWSEDRPVSK+SPL+I HEYEV
Sbjct:  556 LLLYASTLSSQGSHELQHILPRGIWLRFDFEDSHPDLPTLYLQGGSIISLAPPHLHVGEFSLSDDLTLLVSLDENGKAKGLLFEDDGDGYGYTKGRFLVTHYIAERHSSTVTVKVSKTEGDWQRPKRRIHVQLLLGGGAMLDAWGMDGEIIHIKVPSESEVSELISTSNERFKLHMENTKLIPEKEVLPGQKGMELSKEPVELNSGDWKLNIVPWIGGRILSMTHVPSGIQWLHSRIDINGYEEYSGTEYRSAGCTEEYNVIERDLEHAGEEESLILEGDVGGGLVLRRKISIPKENPRVFQIASSIEARSVGAGSGGFSRLVCLRVHPTFTLLHPTESFVSFMSIDGSKHEVWPDSEEQIYEGNNLPHGEWMLVDKSLNLRLVNKFNVSQVFKCIVHWDCGTVNLELWSEDRPVSKESPLKIEHEYEV 984          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy Swiss-Prot
Match: AGL2_BACTQ (Alpha-glucosidase 2 OS=Bacillus thermoamyloliquefaciens PE=3 SV=1)

HSP 1 Score: 410.994 bits (1055), Expect = 1.069e-123
Identity = 214/473 (45.24%), Postives = 291/473 (61.52%), Query Frame = 1

Query:  409 FYGTGEVSGQLERTGKRVFTWNTDAWG-YGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTA--VLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLG 1818
            FYG GE +G L++ G+ +  WNTD +  + PET  LYQSHP+ + V  NG A GI  D T +   D +T    ++        I +  F+ PT   VL   +   G + +PPKW+LGYHQ R+SY++++ V EI++ F EK IP DVI++DI YM+G+R FTFD+ RFP+ K L+ DL   G + + ++DPG+K++  Y +Y  G +HD + +  +G  + G+VWPG   FPDFT  K R WW    + +   G++GIWNDMNEP++F   TKTM    IH  D   G  ++H   HNVYG +M  +TY+GM      KRPF+LTRAGF G QRYAA WTGDN S WEHLQMS+ M + LGLSG    GPD+GGFA N   +L  RW+  GA  P+ R H   G    EPW+FG++ E + +  ++ RY+ +PH+YTLF  AH  G PV  P+FF  P D N   L   FL+G
Sbjct:  149 FYGFGEKTGFLDKRGETMTMWNTDVYAPHNPETDPLYQSHPYFMTVR-NGSAHGIFFDNTYKTTFDFQTATD-EYCFSAEGGAIDYYVFAGPTPKDVLEQYTDLTGRMPLPPKWALGYHQSRYSYETEQEVREIAQTFIEKDIPLDVIYLDIHYMNGYRVFTFDRNRFPNLKQLIADLKQKGIRVVPIVDPGVKEDPEYVIYQEGIRHDYFCKYIEGNVYFGEVWPGKSAFPDFTNKKVRKWWGEKHQFYTDLGIEGIWNDMNEPSVFNE-TKTMDVKVIHDND---GDPKTHRELHNVYGFMMGEATYKGMKKLLNGKRPFLLTRAGFSGIQRYAAVWTGDNRSFWEHLQMSLPMCMNLGLSGVAFCGPDVGGFAHNTNGELLTRWMQVGAFTPYFRNHCAIGFRRQEPWAFGEKYERIIKKYIRLRYQWLPHLYTLFAEAHETGAPVMRPLFFEYPDDENTYNLYDEFLVG 615          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy Swiss-Prot
Match: GANC_MACFA (Neutral alpha-glucosidase C (Fragment) OS=Macaca fascicularis GN=GANC PE=2 SV=2)

HSP 1 Score: 295.819 bits (756), Expect = 1.692e-83
Identity = 178/498 (35.74%), Postives = 263/498 (52.81%), Query Frame = 1

Query:  430 SGQLERTGKR--VFTWNTDAWGYGP-ETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPV------------------------ITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGI---WNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGM-GLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLV 1830
            S QL+ TG       +N D +GY   +   +Y S P++LA    G  +GI         +++ TE  +++      PV                        +  GP  +P+ V    SH  GT  MPP +SLGYHQCRW+Y+ ++ V  +   F E  IP D +W+DI++ +G R FT+DK+RFP+P+ + + L +   K + + DP IK +  Y VY        +++  +G+ F G  WPG   + DFT  K R W+++L    +  G   I   WNDMNEP++F+   +TM ++ IH G+ E      H   HN+YG     +T EG+   +  ++RPFVLTR+ F GSQ+Y A WTGDN + W +L++SI M+L L ++G    G DIGGF GN   +L  RW   GA  PF RGH+       EPW FGKE   + R A++ RY L+P+ Y+LFY AH    PV  P++   P +     +E  ++LG  L+
Sbjct:   89 SHQLKNTGDEDAYRLYNLDVYGYQIYDKMGIYGSVPYLLA-HKLGRTIGIFWLNASETLVEINTEPAVEYTLSQMGPVAAKQKVRSRTHVHWMSESGIIDVFLLTGP--TPSDVFKQYSHLTGTQAMPPLFSLGYHQCRWNYEDEQDVKAVDAGFDEHDIPYDAMWLDIEHTEGKRYFTWDKKRFPNPERMQELLRSKKRKLVVISDPHIKIDPDYSVYVKAKDQGFFVKNQEGEDFEGVCWPGLSSYLDFTNPKVREWYSSLFAFPVYQGSTDILFLWNDMNEPSVFRRPEQTMQKNAIHHGNWE------HRELHNIYGFYHQMATAEGLIQRSGGKERPFVLTRSFFAGSQKYGAVWTGDNTAEWSYLKISIPMLLTLSITGISFCGADIGGFIGNPETELLVRWYQAGAYQPFFRGHATMNAKRREPWLFGKEHTRLIREAIRERYGLLPYWYSLFYHAHVASQPVMRPLWVEFPDELKTFDMEDEYMLGSALL 577          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy Swiss-Prot
Match: GANC_HUMAN (Neutral alpha-glucosidase C OS=Homo sapiens GN=GANC PE=2 SV=3)

HSP 1 Score: 297.745 bits (761), Expect = 4.521e-83
Identity = 179/498 (35.94%), Postives = 263/498 (52.81%), Query Frame = 1

Query:  430 SGQLERTGK--RVFTWNTDAWGYGP-ETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPV------------------------ITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGI---WNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADR-RKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLV 1830
            S QL+ TG       +N D +GY   +   +Y S P++LA    G  +GI         +++ TE  +++      PV                        +  GP  +P+ V    SH  GT  MPP +SLGYHQCRW+Y+ ++ V  +   F E  IP D +W+DI++ +G R FT+DK RFP+PK + + L +   K + + DP IK +  Y VY        +++  +G+ F G  WPG   + DFT  K R W+++L    +  G   I   WNDMNEP++F+   +TM ++ IH G+ E      H   HN+YG     +T EG+    + ++RPFVLTR+ F GSQ+Y A WTGDN + W +L++SI M+L L ++G    G DIGGF GN   +L  RW   GA  PF RGH+   T   EPW FG+E   + R A++ RY L+P+ Y+LFY AH    PV  P++   P +     +E  ++LG  L+
Sbjct:  234 SHQLKNTGDGDAYRLYNLDVYGYQIYDKMGIYGSVPYLLA-HKLGRTIGIFWLNASETLVEINTEPAVEYTLTQMGPVAAKQKVRSRTHVHWMSESGIIDVFLLTGP--TPSDVFKQYSHLTGTQAMPPLFSLGYHQCRWNYEDEQDVKAVDAGFDEHDIPYDAMWLDIEHTEGKRYFTWDKNRFPNPKRMQELLRSKKRKLVVISDPHIKIDPDYSVYVKAKDQGFFVKNQEGEDFEGVCWPGLSSYLDFTNPKVREWYSSLFAFPVYQGSTDILFLWNDMNEPSVFRGPEQTMQKNAIHHGNWE------HRELHNIYGFYHQMATAEGLIKRSKGKERPFVLTRSFFAGSQKYGAVWTGDNTAEWSNLKISIPMLLTLSITGISFCGADIGGFIGNPETELLVRWYQAGAYQPFFRGHATMNTKRREPWLFGEEHTRLIREAIRERYGLLPYWYSLFYHAHVASQPVMRPLWVEFPDELKTFDMEDEYMLGSALL 722          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy Swiss-Prot
Match: GANC_MOUSE (Neutral alpha-glucosidase C OS=Mus musculus GN=Ganc PE=2 SV=2)

HSP 1 Score: 293.508 bits (750), Expect = 9.446e-82
Identity = 172/491 (35.03%), Postives = 258/491 (52.55%), Query Frame = 1

Query:  445 RTGKRVFTWNTDAWGYG-PETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPV------------------------ITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGI---WNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADR-RKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLV 1830
            R G     +N D +GY   +   +Y S P++LA    G  +GI         +++ TE  +++      P                         +  GP  +P  V    S+  GT  MPP +SLGYHQCRW+Y+ ++ V  +   F E  IP DV+W+DI++ +  + FT+DK+RF +PK + + L +   K + + DP IK +  Y VY    +   +++  +G  F G  WPG   + DFT  K R W+++L    +  G   I   WNDMNEP++F+    TM +S +H GD E      H   HN+YG     +T EG+    + ++RPFVL+R+ F GSQ+Y A WTGDN + W +L++SI M+L L +SG    G D+GGF GN   +L  RW   GA  PF RGH+   T   EPW FG+E  ++ R A+++RY L+P++Y+LFY  H    PV  P++   P D     +E  ++LG  L+
Sbjct:  225 RDGDAYRLYNLDVYGYQVHDKMGIYGSVPYLLA-HKQGRTVGIFWLNASETLVEINTEPAVEYTLTQMGPAAAKPKVRCRTDVHWMSESGIIDVFLLTGP--TPADVFKQYSYITGTQAMPPLFSLGYHQCRWNYEDEQDVKAVDAGFDEHDIPYDVMWLDIEHTEDKKYFTWDKKRFANPKRMQELLRSKKRKLVVISDPHIKVDPDYTVYAQAKEQGFFVKNPEGGDFEGVCWPGLSSYLDFTNPKVREWYSSLFAFPVYQGSTDILFLWNDMNEPSVFRGPELTMHKSAVHYGDWE------HRELHNIYGFYQQMATAEGLIQRSKGKERPFVLSRSFFAGSQKYGAVWTGDNKAEWSYLKISIPMLLTLSVSGISFCGADVGGFIGNPEAELLVRWYQAGAYQPFFRGHATMNTKRREPWLFGEEYTQLIREAIRQRYALLPYLYSLFYHTHVSSQPVMRPLWVEYPDDLETFAVEDEYMLGSALL 706          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy Swiss-Prot
Match: GANAB_MOUSE (Neutral alpha-glucosidase AB OS=Mus musculus GN=Ganab PE=1 SV=1)

HSP 1 Score: 291.967 bits (746), Expect = 5.850e-81
Identity = 165/433 (38.11%), Postives = 241/433 (55.66%), Query Frame = 1

Query:  553 GEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDG------IWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGM-GLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLV 1830
            G+ L  L  +    + D+R       I +     +  GP  S   V    +   GT  +PP +SLGYHQ RW+Y  +  VLE+ + F +  +PCDVIW+DI++ DG R FT+D  RFP P ++++ L +   K + ++DP IK + GY V++    H ++++  DG  + G  WPG   +PDFT  + RAWW+N+   F  +  +G      +WNDMNEP++F     TM +  +H G  E      H   HN+YG+ +  +T +G+   +   +RPFVL+RA F GSQR+ A WTGDN + W+HL++SI M L L L G    G D+GGF  N  P+L  RW   GA  PF R H+   T   EPW    + ++  R AL +RY L+P  YTLFY AH  G PV  P++   P+D +  ++E  F+LG  L+
Sbjct:  336 GKMLDYLQGSGETPQTDIRWMSESGIIDV----FLMLGP--SVFDVFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDDHNMPCDVIWLDIEHADGKRYFTWDPTRFPQPLNMLEHLASKRRKLVAIVDPHIKVDSGYRVHEELRNHGLYVKTRDGSDYEGWCWPGSASYPDFTNPRMRAWWSNM---FSFDNYEGSAPNLYVWNDMNEPSVFNGPEVTMLKDAVHYGGWE------HRDIHNIYGLYVHMATADGLIQRSGGIERPFVLSRAFFSGSQRFGAVWTGDNTAEWDHLKISIPMCLSLALVGLSFCGADVGGFFKNPEPELLVRWYQMGAYQPFFRAHAHLDTGRREPWLLASQYQDAIRDALFQRYSLLPFWYTLFYQAHKEGFPVMRPLWVQYPEDMSTFSIEDQFMLGDALL 753          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy Swiss-Prot
Match: PSL5_ARATH (Probable glucan 1,3-alpha-glucosidase OS=Arabidopsis thaliana GN=PSL5 PE=1 SV=1)

HSP 1 Score: 288.886 bits (738), Expect = 5.883e-80
Identity = 179/477 (37.53%), Postives = 258/477 (54.09%), Query Frame = 1

Query:  469 WNTDAWGYGPETT-SLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTEC--RIQFISLPSFP-------------VITF---GPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLV--KDFI-SNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRK-RPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLV 1830
            +N D + Y  E+   LY S P++++   +G+  G         +ID+          ISLPS               V TF   GP   P  V+   +   GT  MP  ++ GYHQCRW+Y  +E V ++  KF E  IP DV+W+DI++ DG R FT+D   FP P+ + K L   G K + ++DP IK+++ YF++   ++   +++ + GK F G  WPG   + D    + R WW      K+++ S      WNDMNEP++F     TMP   +H     +GG + H   HN YG     +T +G+ + +  K RPFVL+RA F G+QRY A WTGDN + WEHL++SI M+L LGL+G   SG DIGGF GN  P+L  RW   GA +PF RGH+   T   EPW FG+   E+ R A+  RY L+P+ YTLF  A+  G PV  P++   P+D    + +  F++G  L+
Sbjct:  255 FNLDVFEYDHESPFGLYGSIPFMVSHGKSGKTSGFFWLNAAEMQIDVLANGWDAESGISLPSSHSRIDTFWMSEAGIVDTFFFVGP--EPKDVVKQYASVTGTSAMPQLFATGYHQCRWNYKDEEDVAQVDSKFDEHDIPYDVLWLDIEHTDGKRYFTWDSVLFPHPEEMQKKLAAKGRKMVTIVDPHIKRDDSYFLHKEATQMGYYVKDSSGKDFDGWCWPGSSSYIDMLSPEIRKWWGGRFSYKNYVGSTPSLYTWNDMNEPSVFNGPEVTMPRDALH-----VGGVE-HREVHNAYGYYFHMATSDGLVMREEGKDRPFVLSRAIFPGTQRYGAIWTGDNTAEWEHLRVSIPMILTLGLTGITFSGADIGGFFGNPEPELLVRWYQVGAYYPFFRGHAHHDTKRREPWLFGERNTELMRDAIHTRYTLLPYFYTLFREANVTGVPVVRPLWMEFPQDEATFSNDEAFMVGSGLL 723          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy Swiss-Prot
Match: GANAB_MACFA (Neutral alpha-glucosidase AB OS=Macaca fascicularis GN=GANAB PE=2 SV=1)

HSP 1 Score: 287.345 bits (734), Expect = 2.741e-79
Identity = 166/440 (37.73%), Postives = 240/440 (54.55%), Query Frame = 1

Query:  553 GEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDG------IWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGM-GLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYSRFSIS 1851
            G+ +  L  +    + D+R       I +     +  GP  S + V    +   GT  +PP +SLGYHQ RW+Y  +  VLE+ + F E  +PCDVIW+DI++ DG R FT+D  RFP P+++++ L +   K + ++DP IK + GY V+D      ++++  DG  + G  WPG   +PDFT    RAWWAN+   F  +  +G      +WNDMNEP++F     TM +   H G  E      H   HN+YG+ +  +T +G+   +   +RPFVL RA F GSQR+ A WTGDN + W+HL++SI M L LGL G    G D+GGF  N  P+L  RW   GA  PF R H+   T    PW    +  ++ R AL +RY L+P  YTLFY AH  G P+  P++   P+D    +++  +LLG  L+    S S
Sbjct:  336 GKMMDYLQGSGETPQTDVRWMSETGIIDV----FLLLGP--SISDVFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDEHNLPCDVIWLDIEHADGKRYFTWDPSRFPQPRTMLERLASKRRKLVAIVDPHIKVDSGYRVHDELRNLGLYVKTRDGSDYEGWCWPGSAGYPDFTNPTMRAWWANM---FSYDNYEGSAPNLFVWNDMNEPSVFNGPEVTMLKDAQHYGGWE------HRDVHNIYGLYVHMATADGLRQRSGGMERPFVLARAFFAGSQRFGAVWTGDNTAEWDHLKISIPMCLSLGLVGLSFCGADVGGFFKNPEPELLVRWYQMGAYQPFFRAHAHLDTGRRGPWLLPSQHNDIIRDALGQRYSLLPFWYTLFYQAHREGIPIMRPLWVQYPQDVTTFSIDDQYLLGDALLVHPVSDS 760          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy Swiss-Prot
Match: GLU2A_ORYSJ (Probable glucan 1,3-alpha-glucosidase OS=Oryza sativa subsp. japonica GN=Os03g0216600 PE=3 SV=1)

HSP 1 Score: 285.034 bits (728), Expect = 1.221e-78
Identity = 156/393 (39.69%), Postives = 217/393 (55.22%), Query Frame = 1

Query:  673 SSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDG------IWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEG-MGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLV 1830
            S P  V+       GT  MP ++++ YHQCRW+Y  +E V  +   F E  IP DV+W+DI++ DG R FT+D   FP+P+ +   + + G K + ++DP IK++  + +++  +    +++ A GK F G  WPG   +PD    + R WWA+    F      G      IWNDMNEP++F     TMP   +H GD E      H   HN YG     +T +G +   + + RPFVL+RA F GSQRY A WTGDN ++W+HL+ SI M+L LGL+G   SG DIGGF GN  P L  RW   GA +PF RGH+   T   EPW FG+    + R A+  RY L+P+ YTLF  A   G PV  P++   P D         F++GP L+
Sbjct:  338 SEPKDVIKQYISVTGTPSMPQQFAVAYHQCRWNYRDEEDVAGVDSGFDEHDIPYDVLWLDIEHTDGKRYFTWDHSAFPNPEVMQGKIADKGRKMVTIVDPHIKRDSSFHLHEEATAKGYYVKDATGKDFDGWCWPGASSYPDMLNPEIREWWAD---KFSYENYKGSTPTLYIWNDMNEPSVFNGPEVTMPRDAVHYGDVE------HRELHNAYGYYFHMATADGLLKRGEGKDRPFVLSRAFFAGSQRYGAIWTGDNSADWDHLKSSIPMVLTLGLTGMTFSGADIGGFFGNPEPDLLVRWYQVGAFYPFFRGHAHHDTKRREPWLFGERRTALMREAIHMRYSLLPYYYTLFREASVTGVPVMRPLWLEFPDDKETYNNGEAFMVGPSLL 721          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy Swiss-Prot
Match: GANAB_HUMAN (Neutral alpha-glucosidase AB OS=Homo sapiens GN=GANAB PE=1 SV=3)

HSP 1 Score: 285.034 bits (728), Expect = 1.490e-78
Identity = 165/440 (37.50%), Postives = 239/440 (54.32%), Query Frame = 1

Query:  553 GEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDG------IWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGM-GLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYSRFSIS 1851
            G+ +  L  +    + D+R       I +     +  GP  S + V    +   GT  +PP +SLGYHQ RW+Y  +  VLE+ + F +  +PCDVIW+DI++ DG R FT+D  RFP P+++++ L +   K + ++DP IK + GY V++      ++++  DG  + G  WPG   +PDFT    RAWWAN+   F  +  +G      +WNDMNEP++F     TM +   H G  E      H   HN+YG+ +  +T +G+   +   +RPFVL RA F GSQR+ A WTGDN + W+HL++SI M L LGL G    G D+GGF  N  P+L  RW   GA  PF R H+   T   EPW    +  ++ R AL +RY L+P  YTL Y AH  G PV  P++   P+D     ++  +LLG  L+    S S
Sbjct:  336 GKMMDYLQGSGETPQTDVRWMSETGIIDV----FLLLGP--SISDVFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDDHNLPCDVIWLDIEHADGKRYFTWDPSRFPQPRTMLERLASKRRKLVAIVDPHIKVDSGYRVHEELRNLGLYVKTRDGSDYEGWCWPGSAGYPDFTNPTMRAWWANM---FSYDNYEGSAPNLFVWNDMNEPSVFNGPEVTMLKDAQHYGGWE------HRDVHNIYGLYVHMATADGLRQRSGGMERPFVLARAFFAGSQRFGAVWTGDNTAEWDHLKISIPMCLSLGLVGLSFCGADVGGFFKNPEPELLVRWYQMGAYQPFFRAHAHLDTGRREPWLLPSQHNDIIRDALGQRYSLLPFWYTLLYQAHREGIPVMRPLWVQYPQDVTTFNIDDQYLLGDALLVHPVSDS 760          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy Swiss-Prot
Match: LYAG_MOUSE (Lysosomal alpha-glucosidase OS=Mus musculus GN=Gaa PE=1 SV=2)

HSP 1 Score: 283.878 bits (725), Expect = 4.016e-78
Identity = 170/484 (35.12%), Postives = 253/484 (52.27%), Query Frame = 1

Query:  457 RVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSF--PVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEE---GYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISN-GVDGIWNDMNEPAIF-KTVTKTMPESNIHRGD---NELGG-------CQS-------HAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYS 1836
            R+  WN D      + T+LY SHP+ LA+   G A G+    +   ++ L+    + + S        +  GP   P +V+      +G  FMPP W LG+H CRW Y S   V ++         P DV W D+DYMD  R FTF+++ F D   +V++LH  G + + ++DP I        Y  YD G +  V+I    G+P +G VWPG   FPDFT  +   WW ++V +F +    DG+W DMNEP+ F +   +  P + +         +GG       C S       H + HN+YG+  A ++   + +  R  RPFV++R+ F G  RYA  WTGD  S+WEHL  S+  +LQ  L G PL G DI GF G+ + +L  RW   GA +PF R H++  ++  EP+ F +  ++  R A   RY L+P++YTLF+ AH RG  VA P+F   P+DP+  +++   L GP L+ +
Sbjct:  275 RITLWNRDT--PPSQGTNLYGSHPFYLALEDGGLAHGVFLLNSNAMDVILQPSPALTWRSTGGILDVYVFLGP--EPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAIVRQVVENMTRTHFPLDVQWNDLDYMDARRDFTFNQDSFADFPDMVRELHQDGRRYMMIVDPAISSAGPAGSYRPYDEGLRRGVFITNETGQPLIGKVWPGTTAFPDFTNPETLDWWQDMVSEFHAQVPFDGMWLDMNEPSNFVRGSQQGCPNNELENPPYVPGVVGGILQAATICASSHQFLSTHYNLHNLYGLTEAIASSRAL-VKTRGTRPFVISRSTFSGHGRYAGHWTGDVRSSWEHLAYSVPDILQFNLLGVPLVGADICGFIGDTSEELCVRWTQLGAFYPFMRNHNDLNSVPQEPYRFSETAQQAMRKAFALRYALLPYLYTLFHRAHVRGDTVARPLFLEFPEDPSTWSVDRQLLWGPALLIT 753