g.raimondii_cottongen_reftransV1_0000434, g.raimondii_cottongen_reftransV1_0000434 (contig) Gossypium raimondii

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: A0A072UCZ2_MEDTR (Neutral alpha-glucosidase OS=Medicago truncatula GN=MTR_5g026610 PE=3 SV=1)

HSP 1 Score: 957.207 bits (2473), Expect = 0.000e+0
Identity = 462/598 (77.26%), Postives = 519/598 (86.79%), Query Frame = 1

Query:   52 RFNPSPPSSTSKFLFRKKSYSKKLTGRRLISKMTDSE--VKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVM-SNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYS 1836
            R + + P  +S    R+K + +KL     ISKM + E  V + ++D   GKMIF+PIL DGVFRFDCS NDR+A YPS+SF+N+K R+ P+  ++K PSY P+FEC L QQ+V+LE P GTS YGTGEVSGQLERTGKRVFTWNTDAWGYGP T+SLYQSHPWVLAVLPNGEALGILADTTRRCEIDLR E  I+FI+  S+PVITFGPF+SPT VL+SLS AIGTVFMPPKWSLGY QCRWSY S +RVLE+++ FREK IPCDVIWMDIDYMDGFRCFTFDKERF DPKSLV+ LH  GFK IWMLDPGIKQE+GYFVYDSGS++DVW+QKADG  FVGDVWPGPCVFPD+TQSK RAWWANLVKDF+SNGVDGIWNDMNEPA+FK VTKTMPESN+HRGD ELGGCQ+H+ YHNVYG+LMARSTYEGM LA+  +RPFVLTRAGF GSQRYAATWTGDNLS WEHL MSISM+LQLGLSGQPLSGPDIGGFAGNATP+LFGRW+G G++FPFCRGHSE GT DHEPWSFG+ECEEVCRLALKRRYRLIP IYTLFY AHT+G PVATP FFADP DP+LR LE+ FLLGP+LVY+
Sbjct:   37 RLHRNTPFLSSIITLRRKRFIEKL-----ISKMANYEGQVSSGSTDVRTGKMIFEPILNDGVFRFDCSLNDRDAAYPSISFVNSKDRETPITGTHKVPSYTPTFECLLEQQVVQLELPVGTSLYGTGEVSGQLERTGKRVFTWNTDAWGYGPGTSSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRKESTIRFIAPSSYPVITFGPFASPTEVLISLSKAIGTVFMPPKWSLGYQQCRWSYLSDQRVLEVAKTFREKSIPCDVIWMDIDYMDGFRCFTFDKERFRDPKSLVESLHYSGFKGIWMLDPGIKQEKGYFVYDSGSENDVWVQKADGTAFVGDVWPGPCVFPDYTQSKVRAWWANLVKDFVSNGVDGIWNDMNEPAVFKAVTKTMPESNVHRGDGELGGCQNHSFYHNVYGLLMARSTYEGMKLANENRRPFVLTRAGFSGSQRYAATWTGDNLSTWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGSLFPFCRGHSEAGTTDHEPWSFGEECEEVCRLALKRRYRLIPLIYTLFYFAHTKGIPVATPTFFADPTDPSLRKLENSFLLGPVLVYA 629          

HSP 2 Score: 437.958 bits (1125), Expect = 1.001e-129
Identity = 223/335 (66.57%), Postives = 263/335 (78.51%), Query Frame = 2

Query: 2567 SFFILPCFTY*QFIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDG-SGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRL 3568
            SF + P   Y       S   + G DKL+  LPKGIWL FDF D+HPDLPALYL+GG IIP G P QHVGE+NP D+LTL+VALDE GKA+G LFEDDGDGY FT G YLLTHY A+L+ + VTV V +TEG WKRP RRLH+QLL+G  AMLD WG DGE L + +PSE EVSKL+S+S++ ++ RLE    IPDVED  SG KG ELSRTPIEL + DW LK+VPWIGGRIISM+H PSG+QWLH R+EI+GYEEYSG EYRSAGC+EEY+++ R+L HAGEEESVLLEGDIGGGLVLQRQI  PK+   +++I SS++AR VGAGSGGFSR 
Sbjct:  619 SFLLGPVLVY------ASTTRNQGLDKLEVTLPKGIWLGFDFGDAHPDLPALYLKGGSIIPAGLPLQHVGEANPSDELTLLVALDESGKAEGFLFEDDGDGYEFTRGNYLLTHYSAQLQSTAVTVSVHRTEGSWKRPKRRLHIQLLLGGGAMLDTWGVDGEVLHVNLPSEEEVSKLVSTSEKQYKERLEKAIQIPDVEDEVSGPKGMELSRTPIELKSSDWLLKVVPWIGGRIISMIHFPSGTQWLHGRIEISGYEEYSGTEYRSAGCSEEYSIINRELGHAGEEESVLLEGDIGGGLVLQRQICFPKNAANIIQINSSIIARNVGAGSGGFSRF 947          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: G7K6R7_MEDTR (Neutral alpha-glucosidase OS=Medicago truncatula GN=MTR_5g026610 PE=3 SV=1)

HSP 1 Score: 956.436 bits (2471), Expect = 0.000e+0
Identity = 462/598 (77.26%), Postives = 519/598 (86.79%), Query Frame = 1

Query:   52 RFNPSPPSSTSKFLFRKKSYSKKLTGRRLISKMTDSE--VKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVM-SNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYS 1836
            R + + P  +S    R+K + +KL     ISKM + E  V + ++D   GKMIF+PIL DGVFRFDCS NDR+A YPS+SF+N+K R+ P+  ++K PSY P+FEC L QQ+V+LE P GTS YGTGEVSGQLERTGKRVFTWNTDAWGYGP T+SLYQSHPWVLAVLPNGEALGILADTTRRCEIDLR E  I+FI+  S+PVITFGPF+SPT VL+SLS AIGTVFMPPKWSLGY QCRWSY S +RVLE+++ FREK IPCDVIWMDIDYMDGFRCFTFDKERF DPKSLV+ LH  GFK IWMLDPGIKQE+GYFVYDSGS++DVW+QKADG  FVGDVWPGPCVFPD+TQSK RAWWANLVKDF+SNGVDGIWNDMNEPA+FK VTKTMPESN+HRGD ELGGCQ+H+ YHNVYG+LMARSTYEGM LA+  +RPFVLTRAGF GSQRYAATWTGDNLS WEHL MSISM+LQLGLSGQPLSGPDIGGFAGNATP+LFGRW+G G++FPFCRGHSE GT DHEPWSFG+ECEEVCRLALKRRYRLIP IYTLFY AHT+G PVATP FFADP DP+LR LE+ FLLGP+LVY+
Sbjct:   37 RLHRNTPFLSSIITLRRKRFIEKL-----ISKMANYEGQVSSGSTDVRTGKMIFEPILNDGVFRFDCSLNDRDAAYPSISFVNSKDRETPITGTHKVPSYTPTFECLLEQQVVQLELPVGTSLYGTGEVSGQLERTGKRVFTWNTDAWGYGPGTSSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRKESTIRFIAPSSYPVITFGPFASPTEVLISLSKAIGTVFMPPKWSLGYQQCRWSYLSDQRVLEVAKTFREKSIPCDVIWMDIDYMDGFRCFTFDKERFRDPKSLVESLHYSGFKGIWMLDPGIKQEKGYFVYDSGSENDVWVQKADGTAFVGDVWPGPCVFPDYTQSKVRAWWANLVKDFVSNGVDGIWNDMNEPAVFKAVTKTMPESNVHRGDGELGGCQNHSFYHNVYGLLMARSTYEGMKLANENRRPFVLTRAGFSGSQRYAATWTGDNLSTWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGSLFPFCRGHSEAGTTDHEPWSFGEECEEVCRLALKRRYRLIPLIYTLFYFAHTKGIPVATPTFFADPTDPSLRKLENSFLLGPVLVYA 629          

HSP 2 Score: 607.06 bits (1564), Expect = 0.000e+0
Identity = 300/445 (67.42%), Postives = 361/445 (81.12%), Query Frame = 2

Query: 2567 SFFILPCFTY*QFIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDG-SGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRLVCLRVHPTFSLLHPTETFVAFTSIDGTKQEVWPETGEKVYQGNLLPNGEWKLVDKCLGLVLINRFNVGEVYKCLIHWGARTVNLELWSEDRPVSKQSPLQIFHEYEVREI 3898
            SF + P   Y       S   + G DKL+  LPKGIWL FDF D+HPDLPALYL+GG IIP G P QHVGE+NP D+LTL+VALDE GKA+G LFEDDGDGY FT G YLLTHY A+L+ + VTV V +TEG WKRP RRLH+QLL+G  AMLD WG DGE L + +PSE EVSKL+S+S++ ++ RLE    IPDVED  SG KG ELSRTPIEL + DW LK+VPWIGGRIISM+H PSG+QWLH R+EI+GYEEYSG EYRSAGC+EEY+++ R+L HAGEEESVLLEGDIGGGLVLQRQI  PK+   +++I SS++AR VGAGSGGFSRLVCLR+HPTF+LLHP+E+FV+FTSI+G+  EV+P+ GE++++G+L+P+GEWKLVDKCLGL L+NRFNV EV KCL+HW   TVNLELWSE RPVS+QSP+QI H+YEV  I
Sbjct:  619 SFLLGPVLVY------ASTTRNQGLDKLEVTLPKGIWLGFDFGDAHPDLPALYLKGGSIIPAGLPLQHVGEANPSDELTLLVALDESGKAEGFLFEDDGDGYEFTRGNYLLTHYSAQLQSTAVTVSVHRTEGSWKRPKRRLHIQLLLGGGAMLDTWGVDGEVLHVNLPSEEEVSKLVSTSEKQYKERLEKAIQIPDVEDEVSGPKGMELSRTPIELKSSDWLLKVVPWIGGRIISMIHFPSGTQWLHGRIEISGYEEYSGTEYRSAGCSEEYSIINRELGHAGEEESVLLEGDIGGGLVLQRQICFPKNAANIIQINSSIIARNVGAGSGGFSRLVCLRIHPTFNLLHPSESFVSFTSINGSMHEVFPDGGEQIFEGHLIPDGEWKLVDKCLGLALVNRFNVTEVSKCLVHWDFGTVNLELWSESRPVSEQSPIQISHQYEVIRI 1057          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: U5GUB5_POPTR (Glycosyl hydrolase family 31 family protein OS=Populus trichocarpa GN=POPTR_0001s01170g PE=3 SV=1)

HSP 1 Score: 952.97 bits (2462), Expect = 0.000e+0
Identity = 471/612 (76.96%), Postives = 518/612 (84.64%), Query Frame = 1

Query:   37 PLAFSRFNPSPPSSTSKFLF--RKKSYSKKLTGRRLISKMTD-SEVKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVMSNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAI---------GTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYS 1836
            P   S F  S  + TS      RK+  +KKL+ R L+SKM D  + K   +D  +G MIFQPILEDG+FRFDCSA  R A YPSLSFI +  RD P+MS+  PSY P++EC  G+QIVK EFP GT+FYGTGEVSGQLERTGKRVFTWNTDAWGYGP TTSLYQSHPWVLAVLPNGEALG+LADTT RCEIDLR E  IQFI+  S+PV+TFG F+SPT VL SLSHAI         GTVFMPPKWSLGY QCRWSYDS ERV EI+R FREKGIPCDVIWMDIDYMDGFRCFTFD+     P+SLVKDLH+ GFKAIWMLDPGIK+EEGY +YDSGS++D WI+KADG+PFVG+VWPGPCVFPDFTQSK RAWWA LVKDF SNGVDGIWNDMNEPA+FKTVTKTMPESN+H GD E+GGCQ+H+HYHNVYGMLMARSTYEG+ LA+  KRPFVLTRAGFIGSQRYAATWTGDNLSNWEH+ MSISM+LQLGLSGQPLSGPDIGGFAGNATPKLFGRW+G GAMFPFCRGHSE  T DHEPWSFG+ECEEVCRLALKRRYRL+PHIYTLFY+AHT G PVATP FFADPKDP LRT E+ FLLGPLLV+S
Sbjct:   29 PPPHSLFPTSTTTCTSAVTLASRKRRLNKKLSCRGLMSKMADHDQAKVVAADVVSGDMIFQPILEDGIFRFDCSAEARAASYPSLSFIRSSDRDTPIMSHSVPSYTPTYECVSGKQIVKFEFPDGTTFYGTGEVSGQLERTGKRVFTWNTDAWGYGPGTTSLYQSHPWVLAVLPNGEALGVLADTTLRCEIDLRKESIIQFIAPSSYPVVTFGLFASPTDVLKSLSHAIVVYAVSLSSGTVFMPPKWSLGYQQCRWSYDSDERVREIARTFREKGIPCDVIWMDIDYMDGFRCFTFDQAY---PQSLVKDLHDDGFKAIWMLDPGIKKEEGYLIYDSGSENDAWIKKADGEPFVGEVWPGPCVFPDFTQSKVRAWWALLVKDFTSNGVDGIWNDMNEPAVFKTVTKTMPESNLHLGDEEIGGCQNHSHYHNVYGMLMARSTYEGIKLANENKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHVHMSISMVLQLGLSGQPLSGPDIGGFAGNATPKLFGRWMGVGAMFPFCRGHSEKSTNDHEPWSFGEECEEVCRLALKRRYRLLPHIYTLFYLAHTTGIPVATPTFFADPKDPGLRTTENSFLLGPLLVFS 637          

HSP 2 Score: 617.846 bits (1592), Expect = 0.000e+0
Identity = 306/430 (71.16%), Postives = 365/430 (84.88%), Query Frame = 2

Query: 2618 SIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRL--ESIKHIPDVEDGSGHKGG-ELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRLVCLRVHPTFSLLHPTETFVAFTSIDGTKQEVWPETGEKVYQGNLLPNGEWKLVDKCLGLVLINRFNVGEVYKCLIHWGARTVNLELWSEDRPVSKQSPLQIFHEYEVREI 3898
            S + D G D+L P+LPKGIWL FDFDDSHPDLP LYLQGG IIP  PPHQHVGE+N  DDLTL+VALD++G A+G LFED+GDGY FT G YLLT YVAEL+ S VTV+VS+ EG WKRP RRL VQLL+G  AMLD+WG DG+ L+I MP+E+EVS L+S+S++ +R RL  E  KHIP++E+ SG KG  +LS+ P+EL NGDW  K+VPWIGGRIISM HLPSG+QWLHSRVEI+GYEEYSG EYRSAGC+EEY+V++RDLEHA EEES++LEG+IGGGLVL+RQI++ KDNPK+L+I+S ++AR VGAGSGGFSRLVCLRVHP F+LLHPTETFV+FTSIDG+K E+WPE+G++ YQ NLLPNGEW LVD+C GL L+NRFN+ EV+KC IHWG  TVNLELWSEDRPVSKQSPL + H YEVR I
Sbjct:  638 STIADQGMDRLHPVLPKGIWLRFDFDDSHPDLPTLYLQGGSIIPLAPPHQHVGEANLSDDLTLLVALDQNGHAEGLLFEDEGDGYEFTRGGYLLTRYVAELQSSAVTVRVSQMEGSWKRPRRRLRVQLLLGGGAMLDSWGIDGDVLKINMPTEVEVSTLVSTSEKQYRTRLGIECAKHIPELEEVSGPKGVVDLSKVPVELKNGDWIAKVVPWIGGRIISMEHLPSGTQWLHSRVEIDGYEEYSGTEYRSAGCSEEYSVIERDLEHAEEEESLILEGNIGGGLVLRRQISILKDNPKILQIDSGIIARSVGAGSGGFSRLVCLRVHPAFTLLHPTETFVSFTSIDGSKHEIWPESGDQFYQENLLPNGEWMLVDQCQGLALVNRFNINEVFKCYIHWGTGTVNLELWSEDRPVSKQSPLTVSHGYEVRGI 1067          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: A0A059C324_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_E01212 PE=3 SV=1)

HSP 1 Score: 951.429 bits (2458), Expect = 0.000e+0
Identity = 458/568 (80.63%), Postives = 500/568 (88.03%), Query Frame = 1

Query:  127 GRRLISKMTDSEVKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVMSNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLV 1830
            G RL +KMT  E +A  +D TAG MIF+PILE+GVFRFDCSANDR A +PSLSF+N K RD P+  +  PSYIP+++C  GQQIVKLE PAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGP TTSLYQSHPWVLAVLP+GE+LGILADTTRRCEIDLR +  IQF +  S+PVITFGPF+SP AVL SLSHA GTVFMPPKWSLGY QCRWSYDS  RV EI+  FREK IPCDV+WMDIDYMDGFRCFTFDK+RF DPKSLV  LH  GFKAIWMLDPGIK+EEGYFV+DSG K DVW++ ADG PFVG+VWPGPCVFPDFTQSK R WWA LV+DFISNGVDGIWNDMNEPA+FK+VTKTMPESNIHRGD+ELGG Q+H+HYHNVYGMLMARSTYEG+  ++  KRPFVLTRAGF+GSQRYAATWTGDNLSNWEHL MSISM+LQLGLSGQPLSGPDIGGF GNATPKLFGRW+G GAMFPFCRGHSE  T DHEPWSFG+ECEEVCRLALKRRYR +PHIYTLFYMAH  GT VA+P FFADPKDP LRTLE+ FLLGPLLV
Sbjct:   81 GSRLKTKMTIREGQAVAADVTAGDMIFEPILEEGVFRFDCSANDRAAAFPSLSFVNGKDRDTPITIHHVPSYIPTYQCLFGQQIVKLELPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPGTTSLYQSHPWVLAVLPSGESLGILADTTRRCEIDLRNKSIIQFTAPSSYPVITFGPFASPGAVLTSLSHASGTVFMPPKWSLGYQQCRWSYDSDNRVREITSTFREKEIPCDVVWMDIDYMDGFRCFTFDKDRFSDPKSLVDSLHRDGFKAIWMLDPGIKREEGYFVFDSGCKSDVWMKAADGTPFVGEVWPGPCVFPDFTQSKVRHWWAQLVQDFISNGVDGIWNDMNEPAVFKSVTKTMPESNIHRGDDELGGVQNHSHYHNVYGMLMARSTYEGIKSSNENKRPFVLTRAGFVGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFVGNATPKLFGRWMGIGAMFPFCRGHSEMDTNDHEPWSFGEECEEVCRLALKRRYRFLPHIYTLFYMAHKTGTLVASPTFFADPKDPRLRTLENSFLLGPLLV 648          

HSP 2 Score: 464.151 bits (1193), Expect = 5.832e-139
Identity = 221/321 (68.85%), Postives = 269/321 (83.80%), Query Frame = 2

Query: 2603 FIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDGSGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSR 3565
             +   S MP   S+ LQ +LPKGIWL+FDFDD+HPDLP L+LQGG IIP GPPHQHVGE+N  DDLTL+VAL+EHG AKG L+EDDGDGY + EGEYLLTHY+AE E S+VT++V   EG WKRP  RLHV LL+G  AM+DAWG DG+ +QI +PSE EVSK++++S++ +  RLES K IPDVE+ S HKG EL+RTPIEL +G+W  K+VPWIGGRIISM H+PSG+QWLHSR+EINGYEEYSG EYRSAGCTE+Y V++R+LEHAGEEES+++EG+IGGGL +QR I++ KDNPKV RI+SSL+ARKVGAGSGGFSR
Sbjct:  646 LLVCASSMPHQESNDLQHVLPKGIWLTFDFDDAHPDLPLLFLQGGSIIPLGPPHQHVGEANLSDDLTLLVALNEHGTAKGILYEDDGDGYKYLEGEYLLTHYIAERESSLVTIRVYTEEGSWKRPKCRLHVMLLLGGGAMVDAWGMDGDTIQITLPSEDEVSKMVAASEKKYNARLESCKRIPDVEEISSHKGVELARTPIELKSGEWIAKVVPWIGGRIISMEHVPSGTQWLHSRIEINGYEEYSGREYRSAGCTEDYTVIERNLEHAGEEESLMMEGEIGGGLAIQRLISIRKDNPKVFRIDSSLVARKVGAGSGGFSR 966          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: A0A072UDZ0_MEDTR (Neutral alpha-glucosidase OS=Medicago truncatula GN=MTR_5g026610 PE=3 SV=1)

HSP 1 Score: 951.429 bits (2458), Expect = 0.000e+0
Identity = 456/572 (79.72%), Postives = 508/572 (88.81%), Query Frame = 1

Query:  130 RRLISKMTDSE--VKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVM-SNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYS 1836
             +LISKM + E  V + ++D   GKMIF+PIL DGVFRFDCS NDR+A YPS+SF+N+K R+ P+  ++K PSY P+FEC L QQ+V+LE P GTS YGTGEVSGQLERTGKRVFTWNTDAWGYGP T+SLYQSHPWVLAVLPNGEALGILADTTRRCEIDLR E  I+FI+  S+PVITFGPF+SPT VL+SLS AIGTVFMPPKWSLGY QCRWSY S +RVLE+++ FREK IPCDVIWMDIDYMDGFRCFTFDKERF DPKSLV+ LH  GFK IWMLDPGIKQE+GYFVYDSGS++DVW+QKADG  FVGDVWPGPCVFPD+TQSK RAWWANLVKDF+SNGVDGIWNDMNEPA+FK VTKTMPESN+HRGD ELGGCQ+H+ YHNVYG+LMARSTYEGM LA+  +RPFVLTRAGF GSQRYAATWTGDNLS WEHL MSISM+LQLGLSGQPLSGPDIGGFAGNATP+LFGRW+G G++FPFCRGHSE GT DHEPWSFG+ECEEVCRLALKRRYRLIP IYTLFY AHT+G PVATP FFADP DP+LR LE+ FLLGP+LVY+
Sbjct:   98 EKLISKMANYEGQVSSGSTDVRTGKMIFEPILNDGVFRFDCSLNDRDAAYPSISFVNSKDRETPITGTHKVPSYTPTFECLLEQQVVQLELPVGTSLYGTGEVSGQLERTGKRVFTWNTDAWGYGPGTSSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRKESTIRFIAPSSYPVITFGPFASPTEVLISLSKAIGTVFMPPKWSLGYQQCRWSYLSDQRVLEVAKTFREKSIPCDVIWMDIDYMDGFRCFTFDKERFRDPKSLVESLHYSGFKGIWMLDPGIKQEKGYFVYDSGSENDVWVQKADGTAFVGDVWPGPCVFPDYTQSKVRAWWANLVKDFVSNGVDGIWNDMNEPAVFKAVTKTMPESNVHRGDGELGGCQNHSFYHNVYGLLMARSTYEGMKLANENRRPFVLTRAGFSGSQRYAATWTGDNLSTWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGSLFPFCRGHSEAGTTDHEPWSFGEECEEVCRLALKRRYRLIPLIYTLFYFAHTKGIPVATPTFFADPTDPSLRKLENSFLLGPVLVYA 669          

HSP 2 Score: 607.06 bits (1564), Expect = 0.000e+0
Identity = 300/445 (67.42%), Postives = 361/445 (81.12%), Query Frame = 2

Query: 2567 SFFILPCFTY*QFIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDG-SGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRLVCLRVHPTFSLLHPTETFVAFTSIDGTKQEVWPETGEKVYQGNLLPNGEWKLVDKCLGLVLINRFNVGEVYKCLIHWGARTVNLELWSEDRPVSKQSPLQIFHEYEVREI 3898
            SF + P   Y       S   + G DKL+  LPKGIWL FDF D+HPDLPALYL+GG IIP G P QHVGE+NP D+LTL+VALDE GKA+G LFEDDGDGY FT G YLLTHY A+L+ + VTV V +TEG WKRP RRLH+QLL+G  AMLD WG DGE L + +PSE EVSKL+S+S++ ++ RLE    IPDVED  SG KG ELSRTPIEL + DW LK+VPWIGGRIISM+H PSG+QWLH R+EI+GYEEYSG EYRSAGC+EEY+++ R+L HAGEEESVLLEGDIGGGLVLQRQI  PK+   +++I SS++AR VGAGSGGFSRLVCLR+HPTF+LLHP+E+FV+FTSI+G+  EV+P+ GE++++G+L+P+GEWKLVDKCLGL L+NRFNV EV KCL+HW   TVNLELWSE RPVS+QSP+QI H+YEV  I
Sbjct:  659 SFLLGPVLVY------ASTTRNQGLDKLEVTLPKGIWLGFDFGDAHPDLPALYLKGGSIIPAGLPLQHVGEANPSDELTLLVALDESGKAEGFLFEDDGDGYEFTRGNYLLTHYSAQLQSTAVTVSVHRTEGSWKRPKRRLHIQLLLGGGAMLDTWGVDGEVLHVNLPSEEEVSKLVSTSEKQYKERLEKAIQIPDVEDEVSGPKGMELSRTPIELKSSDWLLKVVPWIGGRIISMIHFPSGTQWLHGRIEISGYEEYSGTEYRSAGCSEEYSIINRELGHAGEEESVLLEGDIGGGLVLQRQICFPKNAANIIQINSSIIARNVGAGSGGFSRLVCLRIHPTFNLLHPSESFVSFTSINGSMHEVFPDGGEQIFEGHLIPDGEWKLVDKCLGLALVNRFNVTEVSKCLVHWDFGTVNLELWSESRPVSEQSPIQISHQYEVIRI 1097          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: A0A059C3H7_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_E01212 PE=3 SV=1)

HSP 1 Score: 950.273 bits (2455), Expect = 0.000e+0
Identity = 458/568 (80.63%), Postives = 500/568 (88.03%), Query Frame = 1

Query:  127 GRRLISKMTDSEVKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVMSNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLV 1830
            G RL +KMT  E +A  +D TAG MIF+PILE+GVFRFDCSANDR A +PSLSF+N K RD P+  +  PSYIP+++C  GQQIVKLE PAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGP TTSLYQSHPWVLAVLP+GE+LGILADTTRRCEIDLR +  IQF +  S+PVITFGPF+SP AVL SLSHA GTVFMPPKWSLGY QCRWSYDS  RV EI+  FREK IPCDV+WMDIDYMDGFRCFTFDK+RF DPKSLV  LH  GFKAIWMLDPGIK+EEGYFV+DSG K DVW++ ADG PFVG+VWPGPCVFPDFTQSK R WWA LV+DFISNGVDGIWNDMNEPA+FK+VTKTMPESNIHRGD+ELGG Q+H+HYHNVYGMLMARSTYEG+  ++  KRPFVLTRAGF+GSQRYAATWTGDNLSNWEHL MSISM+LQLGLSGQPLSGPDIGGF GNATPKLFGRW+G GAMFPFCRGHSE  T DHEPWSFG+ECEEVCRLALKRRYR +PHIYTLFYMAH  GT VA+P FFADPKDP LRTLE+ FLLGPLLV
Sbjct:   81 GSRLKTKMTIREGQAVAADVTAGDMIFEPILEEGVFRFDCSANDRAAAFPSLSFVNGKDRDTPITIHHVPSYIPTYQCLFGQQIVKLELPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPGTTSLYQSHPWVLAVLPSGESLGILADTTRRCEIDLRNKSIIQFTAPSSYPVITFGPFASPGAVLTSLSHASGTVFMPPKWSLGYQQCRWSYDSDNRVREITSTFREKEIPCDVVWMDIDYMDGFRCFTFDKDRFSDPKSLVDSLHRDGFKAIWMLDPGIKREEGYFVFDSGCKSDVWMKAADGTPFVGEVWPGPCVFPDFTQSKVRHWWAQLVQDFISNGVDGIWNDMNEPAVFKSVTKTMPESNIHRGDDELGGVQNHSHYHNVYGMLMARSTYEGIKSSNENKRPFVLTRAGFVGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFVGNATPKLFGRWMGIGAMFPFCRGHSEMDTNDHEPWSFGEECEEVCRLALKRRYRFLPHIYTLFYMAHKTGTLVASPTFFADPKDPRLRTLENSFLLGPLLV 648          

HSP 2 Score: 645.195 bits (1663), Expect = 0.000e+0
Identity = 300/429 (69.93%), Postives = 368/429 (85.78%), Query Frame = 2

Query: 2603 FIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDGSGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRLVCLRVHPTFSLLHPTETFVAFTSIDGTKQEVWPETGEKVYQGNLLPNGEWKLVDKCLGLVLINRFNVGEVYKCLIHWGARTVNLELWSEDRPVSKQSPLQIFHEYEV 3889
             +   S MP   S+ LQ +LPKGIWL+FDFDD+HPDLP L+LQGG IIP GPPHQHVGE+N  DDLTL+VAL+EHG AKG L+EDDGDGY + EGEYLLTHY+AE E S+VT++V   EG WKRP  RLHV LL+G  AM+DAWG DG+ +QI +PSE EVSK++++S++ +  RLES K IPDVE+ S HKG EL+RTPIEL +G+W  K+VPWIGGRIISM H+PSG+QWLHSR+EINGYEEYSG EYRSAGCTE+Y V++R+LEHAGEEES+++EG+IGGGL +QR I++ KDNPKV RI+SSL+ARKVGAGSGGFSRLVCLRVHPTF+LLHPTE+F++FT++DG+K E+WP++GE+ ++GNLLPNGEW L DKCLG+ L+NRFNV EV+KCLIHWG  TVNLELWSE+RPVSK+SPL++ HEYEV
Sbjct:  646 LLVCASSMPHQESNDLQHVLPKGIWLTFDFDDAHPDLPLLFLQGGSIIPLGPPHQHVGEANLSDDLTLLVALNEHGTAKGILYEDDGDGYKYLEGEYLLTHYIAERESSLVTIRVYTEEGSWKRPKCRLHVMLLLGGGAMVDAWGMDGDTIQITLPSEDEVSKMVAASEKKYNARLESCKRIPDVEEISSHKGVELARTPIELKSGEWIAKVVPWIGGRIISMEHVPSGTQWLHSRIEINGYEEYSGREYRSAGCTEDYTVIERNLEHAGEEESLMMEGEIGGGLAIQRLISIRKDNPKVFRIDSSLVARKVGAGSGGFSRLVCLRVHPTFTLLHPTESFISFTAVDGSKHELWPDSGEQTFEGNLLPNGEWSLWDKCLGVGLVNRFNVNEVFKCLIHWGTGTVNLELWSEERPVSKESPLKVSHEYEV 1074          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: A0A072UP95_MEDTR (Neutral alpha-glucosidase OS=Medicago truncatula GN=MTR_5g026610 PE=3 SV=1)

HSP 1 Score: 947.962 bits (2449), Expect = 0.000e+0
Identity = 450/562 (80.07%), Postives = 502/562 (89.32%), Query Frame = 1

Query:  154 DSEVKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVM-SNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYS 1836
            + +V + ++D   GKMIF+PIL DGVFRFDCS NDR+A YPS+SF+N+K R+ P+  ++K PSY P+FEC L QQ+V+LE P GTS YGTGEVSGQLERTGKRVFTWNTDAWGYGP T+SLYQSHPWVLAVLPNGEALGILADTTRRCEIDLR E  I+FI+  S+PVITFGPF+SPT VL+SLS AIGTVFMPPKWSLGY QCRWSY S +RVLE+++ FREK IPCDVIWMDIDYMDGFRCFTFDKERF DPKSLV+ LH  GFK IWMLDPGIKQE+GYFVYDSGS++DVW+QKADG  FVGDVWPGPCVFPD+TQSK RAWWANLVKDF+SNGVDGIWNDMNEPA+FK VTKTMPESN+HRGD ELGGCQ+H+ YHNVYG+LMARSTYEGM LA+  +RPFVLTRAGF GSQRYAATWTGDNLS WEHL MSISM+LQLGLSGQPLSGPDIGGFAGNATP+LFGRW+G G++FPFCRGHSE GT DHEPWSFG+ECEEVCRLALKRRYRLIP IYTLFY AHT+G PVATP FFADP DP+LR LE+ FLLGP+LVY+
Sbjct:    5 EGQVSSGSTDVRTGKMIFEPILNDGVFRFDCSLNDRDAAYPSISFVNSKDRETPITGTHKVPSYTPTFECLLEQQVVQLELPVGTSLYGTGEVSGQLERTGKRVFTWNTDAWGYGPGTSSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRKESTIRFIAPSSYPVITFGPFASPTEVLISLSKAIGTVFMPPKWSLGYQQCRWSYLSDQRVLEVAKTFREKSIPCDVIWMDIDYMDGFRCFTFDKERFRDPKSLVESLHYSGFKGIWMLDPGIKQEKGYFVYDSGSENDVWVQKADGTAFVGDVWPGPCVFPDYTQSKVRAWWANLVKDFVSNGVDGIWNDMNEPAVFKAVTKTMPESNVHRGDGELGGCQNHSFYHNVYGLLMARSTYEGMKLANENRRPFVLTRAGFSGSQRYAATWTGDNLSTWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGSLFPFCRGHSEAGTTDHEPWSFGEECEEVCRLALKRRYRLIPLIYTLFYFAHTKGIPVATPTFFADPTDPSLRKLENSFLLGPVLVYA 566          

HSP 2 Score: 437.187 bits (1123), Expect = 3.741e-130
Identity = 223/335 (66.57%), Postives = 263/335 (78.51%), Query Frame = 2

Query: 2567 SFFILPCFTY*QFIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDG-SGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRL 3568
            SF + P   Y       S   + G DKL+  LPKGIWL FDF D+HPDLPALYL+GG IIP G P QHVGE+NP D+LTL+VALDE GKA+G LFEDDGDGY FT G YLLTHY A+L+ + VTV V +TEG WKRP RRLH+QLL+G  AMLD WG DGE L + +PSE EVSKL+S+S++ ++ RLE    IPDVED  SG KG ELSRTPIEL + DW LK+VPWIGGRIISM+H PSG+QWLH R+EI+GYEEYSG EYRSAGC+EEY+++ R+L HAGEEESVLLEGDIGGGLVLQRQI  PK+   +++I SS++AR VGAGSGGFSR 
Sbjct:  556 SFLLGPVLVY------ASTTRNQGLDKLEVTLPKGIWLGFDFGDAHPDLPALYLKGGSIIPAGLPLQHVGEANPSDELTLLVALDESGKAEGFLFEDDGDGYEFTRGNYLLTHYSAQLQSTAVTVSVHRTEGSWKRPKRRLHIQLLLGGGAMLDTWGVDGEVLHVNLPSEEEVSKLVSTSEKQYKERLEKAIQIPDVEDEVSGPKGMELSRTPIELKSSDWLLKVVPWIGGRIISMIHFPSGTQWLHGRIEISGYEEYSGTEYRSAGCSEEYSIINRELGHAGEEESVLLEGDIGGGLVLQRQICFPKNAANIIQINSSIIARNVGAGSGGFSRF 884          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: A0A072UEX7_MEDTR (Neutral alpha-glucosidase OS=Medicago truncatula GN=MTR_5g026610 PE=3 SV=1)

HSP 1 Score: 946.421 bits (2445), Expect = 0.000e+0
Identity = 450/562 (80.07%), Postives = 502/562 (89.32%), Query Frame = 1

Query:  154 DSEVKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVM-SNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYS 1836
            + +V + ++D   GKMIF+PIL DGVFRFDCS NDR+A YPS+SF+N+K R+ P+  ++K PSY P+FEC L QQ+V+LE P GTS YGTGEVSGQLERTGKRVFTWNTDAWGYGP T+SLYQSHPWVLAVLPNGEALGILADTTRRCEIDLR E  I+FI+  S+PVITFGPF+SPT VL+SLS AIGTVFMPPKWSLGY QCRWSY S +RVLE+++ FREK IPCDVIWMDIDYMDGFRCFTFDKERF DPKSLV+ LH  GFK IWMLDPGIKQE+GYFVYDSGS++DVW+QKADG  FVGDVWPGPCVFPD+TQSK RAWWANLVKDF+SNGVDGIWNDMNEPA+FK VTKTMPESN+HRGD ELGGCQ+H+ YHNVYG+LMARSTYEGM LA+  +RPFVLTRAGF GSQRYAATWTGDNLS WEHL MSISM+LQLGLSGQPLSGPDIGGFAGNATP+LFGRW+G G++FPFCRGHSE GT DHEPWSFG+ECEEVCRLALKRRYRLIP IYTLFY AHT+G PVATP FFADP DP+LR LE+ FLLGP+LVY+
Sbjct:    5 EGQVSSGSTDVRTGKMIFEPILNDGVFRFDCSLNDRDAAYPSISFVNSKDRETPITGTHKVPSYTPTFECLLEQQVVQLELPVGTSLYGTGEVSGQLERTGKRVFTWNTDAWGYGPGTSSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRKESTIRFIAPSSYPVITFGPFASPTEVLISLSKAIGTVFMPPKWSLGYQQCRWSYLSDQRVLEVAKTFREKSIPCDVIWMDIDYMDGFRCFTFDKERFRDPKSLVESLHYSGFKGIWMLDPGIKQEKGYFVYDSGSENDVWVQKADGTAFVGDVWPGPCVFPDYTQSKVRAWWANLVKDFVSNGVDGIWNDMNEPAVFKAVTKTMPESNVHRGDGELGGCQNHSFYHNVYGLLMARSTYEGMKLANENRRPFVLTRAGFSGSQRYAATWTGDNLSTWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGSLFPFCRGHSEAGTTDHEPWSFGEECEEVCRLALKRRYRLIPLIYTLFYFAHTKGIPVATPTFFADPTDPSLRKLENSFLLGPVLVYA 566          

HSP 2 Score: 606.29 bits (1562), Expect = 0.000e+0
Identity = 300/445 (67.42%), Postives = 361/445 (81.12%), Query Frame = 2

Query: 2567 SFFILPCFTY*QFIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDG-SGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRLVCLRVHPTFSLLHPTETFVAFTSIDGTKQEVWPETGEKVYQGNLLPNGEWKLVDKCLGLVLINRFNVGEVYKCLIHWGARTVNLELWSEDRPVSKQSPLQIFHEYEVREI 3898
            SF + P   Y       S   + G DKL+  LPKGIWL FDF D+HPDLPALYL+GG IIP G P QHVGE+NP D+LTL+VALDE GKA+G LFEDDGDGY FT G YLLTHY A+L+ + VTV V +TEG WKRP RRLH+QLL+G  AMLD WG DGE L + +PSE EVSKL+S+S++ ++ RLE    IPDVED  SG KG ELSRTPIEL + DW LK+VPWIGGRIISM+H PSG+QWLH R+EI+GYEEYSG EYRSAGC+EEY+++ R+L HAGEEESVLLEGDIGGGLVLQRQI  PK+   +++I SS++AR VGAGSGGFSRLVCLR+HPTF+LLHP+E+FV+FTSI+G+  EV+P+ GE++++G+L+P+GEWKLVDKCLGL L+NRFNV EV KCL+HW   TVNLELWSE RPVS+QSP+QI H+YEV  I
Sbjct:  556 SFLLGPVLVY------ASTTRNQGLDKLEVTLPKGIWLGFDFGDAHPDLPALYLKGGSIIPAGLPLQHVGEANPSDELTLLVALDESGKAEGFLFEDDGDGYEFTRGNYLLTHYSAQLQSTAVTVSVHRTEGSWKRPKRRLHIQLLLGGGAMLDTWGVDGEVLHVNLPSEEEVSKLVSTSEKQYKERLEKAIQIPDVEDEVSGPKGMELSRTPIELKSSDWLLKVVPWIGGRIISMIHFPSGTQWLHGRIEISGYEEYSGTEYRSAGCSEEYSIINRELGHAGEEESVLLEGDIGGGLVLQRQICFPKNAANIIQINSSIIARNVGAGSGGFSRLVCLRIHPTFNLLHPSESFVSFTSINGSMHEVFPDGGEQIFEGHLIPDGEWKLVDKCLGLALVNRFNVTEVSKCLVHWDFGTVNLELWSESRPVSEQSPIQISHQYEVIRI 994          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: K4CMS0_SOLLC (Uncharacterized protein OS=Solanum lycopersicum GN=Solyc08g075530.2 PE=3 SV=1)

HSP 1 Score: 944.495 bits (2440), Expect = 0.000e+0
Identity = 452/584 (77.40%), Postives = 509/584 (87.16%), Query Frame = 1

Query:   91 LFRKKSYSKKLTGRRLIS-KMTDSEVKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVMS-NKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYS 1836
            L R +S +K   G   +  KM   E   A SD+  G MIF+ ILE+GVFRFDCSA+DR A +PS+SF++ K R+ P+MS +K PSYIP+FEC  GQQIV +E P+GTSFYGTGEVSGQLERTGKR+ TWNTDAWGYGP TTSLYQSHPWVLAVLP+GE LG+LADTT RCE+DLR E  I+FIS  SFPVITFGPF SP  VLVSLSHAIGTVFMPPKWSLGYHQCRWSY    RV EI+R FREK IPCDVIWMDIDYM+GFRCFTFDKERFPDP+SLV++LH  GFKAIWMLDPGIK E+GYF YDSGS+ DVW+Q ADG+P+VGDVWPGPCVFPDFTQ KAR+WWANLVKDFISNGVDGIWNDMNEPA+FKTVTKTMPE+NIHRGD E GGCQ+H++YHNVYGMLMARSTYEGM LA+  KRPFVLTRAGF+GSQRYAATWTGDNLS WEHLQMSI M+LQLGLSGQPL+GPDIGGFAGNATP++FGRW+G G++FPFCR HSE  T DHEPWSFG+ECEEVCRLAL+RRYRL+PHIYTLFY+AHTRGTPV+ P+FFADPKDP LR LE+ FLLGP+L+Y+
Sbjct:   58 LIRGRSVNKGFIGASFVMLKMGGIEGTTAMSDARTGNMIFESILEEGVFRFDCSADDRNAAFPSISFVDPKVRETPLMSIHKVPSYIPTFECVRGQQIVNIELPSGTSFYGTGEVSGQLERTGKRILTWNTDAWGYGPGTTSLYQSHPWVLAVLPSGETLGVLADTTHRCEVDLRQESNIRFISRQSFPVITFGPFPSPIDVLVSLSHAIGTVFMPPKWSLGYHQCRWSYVPDTRVREIARTFREKKIPCDVIWMDIDYMNGFRCFTFDKERFPDPESLVEELHKSGFKAIWMLDPGIKNEKGYFAYDSGSEADVWVQTADGRPYVGDVWPGPCVFPDFTQLKARSWWANLVKDFISNGVDGIWNDMNEPAVFKTVTKTMPENNIHRGDPEFGGCQNHSYYHNVYGMLMARSTYEGMKLANGNKRPFVLTRAGFVGSQRYAATWTGDNLSTWEHLQMSIPMVLQLGLSGQPLTGPDIGGFAGNATPRMFGRWMGVGSLFPFCRAHSEADTNDHEPWSFGEECEEVCRLALERRYRLLPHIYTLFYLAHTRGTPVSAPIFFADPKDPELRKLENSFLLGPILIYA 641          

HSP 2 Score: 625.55 bits (1612), Expect = 0.000e+0
Identity = 299/444 (67.34%), Postives = 367/444 (82.66%), Query Frame = 2

Query: 2567 SFFILPCFTY*QFIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDGSGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRLVCLRVHPTFSLLHPTETFVAFTSIDGTKQEVWPETGEKVYQGNLLPNGEWKLVDKCLGLVLINRFNVGEVYKCLIHWGARTVNLELWSEDRPVSKQSPLQIFHEYEVREI 3898
            SF + P   Y       S   D   D     LP+GIWLSFDFDDSHPDLPALYL GG IIP GP +QHVG+++P DDLTL++ALDE+GKA+G LFEDDGDGY +++G YLLT YVAEL+ SVVTV+V+KTEG W+RP RRLHV++L+G+ AMLDAWG+DGE +Q+ MPSE +VS L+S S+E +R RLE  K IPDVE  SGHKG ELSRTP+ L +GDW LK VPWIGGRI+SM H+PSG+QWLHSRVEINGYEEYS  EYRSAGCTEEY+V++RDLE  GE ES+ LEGDIGGGL ++R I++PKDN KV RI+S ++AR VGAGSGGFSRLVCLRVHP F+LLHPTE++V+FTS++G+K E+WPE+GE+V++G+L P GEW LVD+CLGL L+NRFN+ +V+KC++HWG  TVNLELWSE+RPVSK SPL+I HEYEV++I
Sbjct:  631 SFLLGPILIY------ASTQRDEELDTAHHKLPRGIWLSFDFDDSHPDLPALYLLGGSIIPVGPLYQHVGQADPSDDLTLLIALDENGKAEGLLFEDDGDGYEYSQGGYLLTTYVAELQSSVVTVQVAKTEGNWRRPKRRLHVRILLGKGAMLDAWGSDGEIIQLAMPSETDVSNLVSESEEKYRNRLEGAKRIPDVETISGHKGVELSRTPVVLKSGDWELKAVPWIGGRILSMDHVPSGTQWLHSRVEINGYEEYSNREYRSAGCTEEYSVIERDLEQEGESESLRLEGDIGGGLFMERYISLPKDNSKVFRIDSGIVARGVGAGSGGFSRLVCLRVHPMFTLLHPTESYVSFTSLNGSKHELWPESGEQVFEGDLRPKGEWMLVDRCLGLGLVNRFNIDQVHKCMVHWGTGTVNLELWSEERPVSKDSPLKISHEYEVQKI 1068          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy TrEMBL
Match: D7L3E3_ARALL (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_898886 PE=3 SV=1)

HSP 1 Score: 924.85 bits (2389), Expect = 0.000e+0
Identity = 442/573 (77.14%), Postives = 500/573 (87.26%), Query Frame = 1

Query:  163 VKAATSDSTAGKMIFQPILEDGVFRFDCSANDREAVYPSLSFINNKSRDVPVMSNKTPSYIPSFECRLGQQIVKLEFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDGIWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYSRFSISPFKDEFYRIV 1881
            V   +S++ +  MIF+PILE GVFRFD S + R+AV+PS+SF N+K R+VP++S+  P+YIP+  C   QQ+V  EF  GTSFYGTGEVSGQLERTGKRVFTWNTDAWGYG  TTSLYQSHPWVL VLP GE LG+LADTTR+CEIDLR E  I+ IS  S+P+ITFGPFSSPTAVL SLSHAIGTVFMPPKW+LGYHQCRWSY S +RV EI++ FR+K IP DVIWMDIDYMDGFRCFTFDKERFPDP +L KDLH+ GFKAIWMLDPGIKQEEGY+VYDSGSK+DVWI +ADGKPF+G+VWPGPCVFPD+T SKAR+WWANLVK+F+SNGVDGIWNDMNEPA+FK VTKTMPE+NIHRGD++LGG Q+H+HYHNVYGMLMARSTYEGM LAD+ KRPFVLTRAGFIGSQRYAATWTGDNLSNWEHL MSISM+LQLGLSGQPLSGPDIGGFAGNATP+LFGRW+G GAMFPFCRGHSE GT DHEPWSFG+ECEEVCR ALKRRY+L+PH YTLFY+AHT G PVA P+FFADPKD  LRT+E+ FLLGPLL+Y+    S    E   I+
Sbjct:    3 VSGDSSETVSTDMIFEPILEHGVFRFDSSVDHRKAVFPSVSFKNSKDREVPIVSHSVPAYIPTSVCLQDQQVVTFEFSPGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGSGTTSLYQSHPWVLVVLPTGETLGVLADTTRKCEIDLRKEGIIRIISPTSYPIITFGPFSSPTAVLESLSHAIGTVFMPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDIDYMDGFRCFTFDKERFPDPSALTKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWISRADGKPFIGEVWPGPCVFPDYTNSKARSWWANLVKEFVSNGVDGIWNDMNEPAVFKVVTKTMPENNIHRGDDDLGGVQNHSHYHNVYGMLMARSTYEGMELADKNKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGAMFPFCRGHSEAGTADHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYIAHTTGAPVAAPIFFADPKDSRLRTVENGFLLGPLLLYASTLSSQGSHELQHIL 575          

HSP 2 Score: 621.313 bits (1601), Expect = 0.000e+0
Identity = 294/429 (68.53%), Postives = 359/429 (83.68%), Query Frame = 2

Query: 2603 FIFVISIMPDLGSDKLQPLLPKGIWLSFDFDDSHPDLPALYLQGGYIIPFGPPHQHVGESNPLDDLTLIVALDEHGKAKGSLFEDDGDGYGFTEGEYLLTHYVAELECSVVTVKVSKTEGLWKRPNRRLHVQLLIGEDAMLDAWGNDGEDLQIEMPSEIEVSKLISSSKEHHRLRLESIKHIPDVEDGSGHKGGELSRTPIELANGDWSLKIVPWIGGRIISMVHLPSGSQWLHSRVEINGYEEYSGMEYRSAGCTEEYNVMQRDLEHAGEEESVLLEGDIGGGLVLQRQITVPKDNPKVLRIESSLLARKVGAGSGGFSRLVCLRVHPTFSLLHPTETFVAFTSIDGTKQEVWPETGEKVYQGNLLPNGEWKLVDKCLGLVLINRFNVGEVYKCLIHWGARTVNLELWSEDRPVSKQSPLQIFHEYEV 3889
             +   S +   GS +LQ +LP+GIWL FDF+DSHPDLP LYLQGG II   PPH HVGE +  DDLTL+V+LDE+GKAKG LFEDDGDGYG+T+G +L+THY+AE   S VTVKVSKTEG W+RP RR+HVQLL+G  AMLDAWG DGE + I++PSE EVS+LIS+S E  +L +E+ K IP+ E   G KG ELS+ P+EL +GDW L IVPWIGGRI+SM H+PSG QWLHSR++INGYEEYSG EYRSAGCTEEYNV++RDLEHAGEEES++LEGD+GGGLVL+R+I++PK+NP+V +I SS+ AR VGAGSGGFSRLVCLRVHPTF+LLHPTE+FV+F SIDG+K EVWP++ E++Y+GN LP+GEW LVDK L L L+N+FNV +V+KC++HW   TVNLELWSEDRPVSK+SPL+I HEYEV
Sbjct:  556 LLLYASTLSSQGSHELQHILPRGIWLRFDFEDSHPDLPTLYLQGGSIISLAPPHLHVGEFSLSDDLTLLVSLDENGKAKGLLFEDDGDGYGYTKGRFLVTHYIAERHSSTVTVKVSKTEGDWQRPKRRIHVQLLLGGGAMLDAWGMDGEIIHIKVPSESEVSELISTSNERFKLHMENTKLIPEKEVLPGQKGMELSKEPVELNSGDWKLNIVPWIGGRILSMTHVPSGIQWLHSRIDINGYEEYSGTEYRSAGCTEEYNVIERDLEHAGEEESLILEGDVGGGLVLRRKISIPKENPRVFQIASSIEARSVGAGSGGFSRLVCLRVHPTFTLLHPTESFVSFMSIDGSKHEVWPDSEEQIYEGNNLPHGEWMLVDKSLNLRLVNKFNVSQVFKCIVHWDCGTVNLELWSEDRPVSKESPLKIEHEYEV 984          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy Swiss-Prot
Match: GANAB_DICDI (Neutral alpha-glucosidase AB OS=Dictyostelium discoideum GN=modA PE=3 SV=1)

HSP 1 Score: 268.855 bits (686), Expect = 4.127e-73
Identity = 146/393 (37.15%), Postives = 216/393 (54.96%), Query Frame = 1

Query:  676 SPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISNGVDG------IWNDMNEPAIFKTVTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGL--ADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLV 1830
            +P+ +    ++  GT  +P  +SLGYHQC+W+Y S++ V ++   F E  IP DVIW+DI++ DG R FT+D   FP P  +   +     K + ++DP IK++  Y+V+   +    +I+  DG  + G  WPG   + DFT  + R WWA     F  +   G      IWNDMNEP++F       PE ++H+     GG + H   HN+YG     ++ +G+    AD+  RPFVL+RA + GSQR  A WTGDN + W HL++S  M+L + L+G   SG D+GGF GN   +L  RW   GA  PF RGH+   +   EPW F +    + R A+ +RY  +P  YT FY     G PV  P++   PK+ NL  ++  +L+G  L+
Sbjct:  369 TPSTIFKQYAYLTGTTALPQMFSLGYHQCKWNYKSEDDVKQVDNGFDENHIPYDVIWLDIEHTDGKRYFTWDNNNFPTPADMQNIIGAKHRKMVTIVDPHIKRDNNYYVHSEATSKGYYIKNKDGNDYDGWCWPGSSSYLDFTNPEIRKWWAT---QFGYDKYKGSTPNLYIWNDMNEPSVFNG-----PEVSMHKDAKHHGGFE-HRDVHNLYGYYYHMASADGLVQRNADQNDRPFVLSRAFYAGSQRIGAIWTGDNSAQWSHLEISNPMLLSMNLAGITFSGADVGGFFGNPDAELLTRWYQAGAFQPFFRGHAHLDSRRREPWLFNEPYTTIIREAIVKRYSYLPLWYTTFYQNTLNGAPVMRPLWVQYPKEANLFDVDDHYLIGDSLL 752          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy Swiss-Prot
Match: AGLU_SPIOL (Alpha-glucosidase OS=Spinacia oleracea PE=1 SV=1)

HSP 1 Score: 267.314 bits (682), Expect = 7.573e-73
Identity = 169/469 (36.03%), Postives = 240/469 (51.17%), Query Frame = 1

Query:  505 TSLYQSHPWVLAVLPN---GEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFP--DPKSLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFIS-NGVDGIWNDMNEPAIFKT-------------------------VTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLG 1818
             +LY SHP+ + V  +   G   G+L   +   +++  T  RI +  +     + F    SP  V+   +  IG     P W+ G+ QCR+ Y     +  +   + +  IP +V+W DIDYMD ++ FT D   FP    K  V +LH  G K + +LDPGI   + Y  Y  G KHDV++++ +GKP++G VWPGP  FPDF +  A  +W + +K F++   VDG+W DMNE + F +                         + KT+P + +H GD          + HN++G L AR T   + +    KRPFVL+R+ F GS +Y A WTGDN + W  L  SI  ML  GL G P+ G DI GF GN T +L  RW+  GA +PF R HS  GT   E + + +      R  L  RY L+P+ YTL Y A   G P+A P+FF+ P D     + S FLLG
Sbjct:  236 VNLYGSHPFYMDVRSSPVAGSTHGVLLLNSNGMDVEY-TGNRITYKVIGGIIDLYFFAGPSPGQVVEQFTRVIGRPAPMPYWAFGFQQCRYGYHDVYELQSVVAGYAKAKIPLEVMWTDIDYMDAYKDFTLDPVNFPLDKMKKFVNNLHKNGQKYVVILDPGISTNKTYETYIRGMKHDVFLKR-NGKPYLGSVWPGPVYFPDFLKPSALTFWTDEIKRFLNLLPVDGLWIDMNEISNFISSPPIPGSTLDNPPYKINNSGVMLPIINKTIPPTAMHYGD------IPEYNVHNLFGYLEARVTRAAL-IKLTEKRPFVLSRSTFSGSGKYTAHWTGDNAATWNDLVYSIPSMLDFGLFGIPMVGADICGFLGNTTEELCRRWIQLGAFYPFSRDHSSLGTTYQELYRW-ESVAASARKVLGLRYTLLPYFYTLMYEAQLNGIPIARPLFFSFPDDIKTYGISSQFLLG 694          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy Swiss-Prot
Match: AGLU_HORVU (Alpha-glucosidase OS=Hordeum vulgare PE=2 SV=1)

HSP 1 Score: 259.996 bits (663), Expect = 1.814e-70
Identity = 178/507 (35.11%), Postives = 254/507 (50.10%), Query Frame = 1

Query:  379 VKLEFPAG-TSFYGTGE--VSGQLERTGKRVFTWNTDAWGYGPETTSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERF--PDPKSLVKDLHNIGFKAIWMLDPGIKQE---EGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISN-GVDGIWNDMNEPAIFKT------------------VTKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLG 1818
            V    PAG  S YG GE   S    R       WN D  G      +LY SHP+ + V   G A G+L  ++   ++ L     + +  +       F    +P AV+   +  I      P WS G+HQCR+ Y +   +  +  ++ +  IP +V+W DIDYMDGF+ FT D+  F   + +  V  LH    K + +LDPGI+ +     Y  +  G + D+++++ +G  FVG+VWPG   FPDF    A  +WA  +  F     VDG+W DMNE + F                      + +    +       GG   +   HN++G+L AR+T  G+ L D  +RPFVL+R+ F+GS RY A WTGDN + W  L+ SI+ ML  GL G P+ G DI GF GN T +L GRW+  GA +PF R HS   T+  E + +        R AL  RY+L+P+ YTL Y AH  G P+A P+FF+ P D     ++  FLLG
Sbjct:  164 VTSALPAGRASLYGLGEHTKSSFRLRHNDSFTLWNADI-GASYVDVNLYGSHPFYMDVRAPGTAHGVLLLSSNGMDV-LYGGSYVTYKVIGGVLDFYFFAGPNPLAVVDQYTQLIARPAPMPYWSFGFHQCRYGYLNVSDLERVVARYAKARIPLEVMWTDIDYMDGFKDFTLDRVNFTAAELRPFVDRLHRNAQKYVLILDPGIRVDPIDATYGTFVRGMQQDIFLKR-NGTNFVGNVWPGDVYFPDFMHPAAAEFWAREISLFRRTIPVDGLWIDMNEISNFYNPEPMNALDDPPYRINNDGTGRPINNKTVRPLAVHYGGVTEYEE-HNLFGLLEARATGRGV-LRDTGRRPFVLSRSTFVGSGRYTAYWTGDNAATWGDLRYSINTMLSFGLFGMPMIGADICGFNGNTTEELCGRWIQLGAFYPFSRDHSAIFTVRRELYLW-PSVAASGRKALGLRYQLLPYFYTLMYEAHMTGAPIARPLFFSYPHDVATYGVDRQFLLG 664          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy Swiss-Prot
Match: AGLU_BETVU (Alpha-glucosidase OS=Beta vulgaris PE=1 SV=1)

HSP 1 Score: 258.84 bits (660), Expect = 6.527e-70
Identity = 170/503 (33.80%), Postives = 252/503 (50.10%), Query Frame = 1

Query:  409 FYGTGE-VSGQLERTGKRVFT-WNTDAWGYGPETTSLYQSHPWVLAVLPN---GEALGILADTTRRCEIDLRTECRIQFISLPSFPVITFGPFSSPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPK--SLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISN-GVDGIWNDMNEPAIFKTV-------------------------TKTMPESNIHRGDNELGGCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLG 1818
             YG GE      +    ++ T WN D   +  +  +LY SHP+ + V  +   G   G+    +   +++  T  RI +  +     +      +P  VL   +  IG     P W+ G+HQCRW Y     +  +  K+ E  IP +V+W DIDYMD F+ FT D   FP  K    V  LH  G + + +LDPGI   + Y  +  G + +V+I++ +G P++G VWPGP  +PDF    AR++W + +K F     +DGIW DMNE + F T                          +KT+P + +H G+       +  + HN+YG L +++T E + +    + PF+L+R+ F GS +Y A WTGDN + W+ LQ SI  ML  GL G P+ G DI GFA + T +L  RW+  GA +PF R HS   T  H+     +      R  L  RY L+P+ YTL Y A+ RG+P+A P+ F  P D     + S FL+G
Sbjct:  207 LYGLGEHTKPTFQLAHNQILTLWNADIASFNRDL-NLYGSHPFYMDVRSSPMVGSTHGVFLLNSNGMDVEY-TGDRITYKVIGGIIDLYIFAGRTPEMVLDQYTKLIGRPAPMPYWAFGFHQCRWGYRDVNEIETVVDKYAEARIPLEVMWTDIDYMDAFKDFTLDPVHFPLDKMQQFVTKLHRNGQRYVPILDPGINTNKSYGTFIRGMQSNVFIKR-NGNPYLGSVWPGPVYYPDFLDPAARSFWVDEIKRFRDILPIDGIWIDMNEASNFITSAPTPGSTLDNPPYKINNSGGRVPINSKTIPATAMHYGN------VTEYNAHNLYGFLESQATREAL-VRPATRGPFLLSRSTFAGSGKYTAHWTGDNAARWDDLQYSIPTMLNFGLFGMPMIGADICGFAESTTEELCCRWIQLGAFYPFSRDHSARDTT-HQELYLWESVAASARTVLGLRYELLPYYYTLMYDANLRGSPIARPLSFTFPDDVATYGISSQFLIG 698          

BLAST of g.raimondii_cottongen_reftransV1_0000434 vs. ExPASy Swiss-Prot
Match: AGLU_MUCJA (Alpha-glucosidase OS=Mucor javanicus PE=1 SV=1)

HSP 1 Score: 256.914 bits (655), Expect = 1.370e-69
Identity = 176/556 (31.65%), Postives = 256/556 (46.04%), Query Frame = 1

Query:  388 EFPAGTSFYGTGEVSGQLERTGKRVFTWNTDAWGYGPET--TSLYQSHPWVLAVLPNGEALGILADTTRRCEIDLRTECRIQFISLPS-FPVITFGPFS-SPTAVLVSLSHAIGTVFMPPKWSLGYHQCRWSYDSQERVLEISRKFREKGIPCDVIWMDIDYMDGFRCFTFDKERFPDPK--SLVKDLHNIGFKAIWMLDPGIKQEEGYFVYDSGSKHDVWIQKADGKPFVGDVWPGPCVFPDFTQSKARAWWANLVKDFISN-GVDGIWNDMNEPAIF------------------KTVTKTMPESNIHRGDNELG--------------------------------------GCQSHAHYHNVYGMLMARSTYEGMGLADRRKRPFVLTRAGFIGSQRYAATWTGDNLSNWEHLQMSISXXXXXXXXXXXXXXXDIGGFAGNATPKLFGRWLGFGAMFPFCRGHSETGTIDHEPWSFGKECEEVCRLALKRRYRLIPHIYTLFYMAHTRGTPVATPVFFADPKDPNLRTLESCFLLGPLLVYSRFSISPFKDE 1866
            + P   + YG GEV+    RT      W  D     P+    ++Y +HP+   V  +G+A G L       ++ + TE RI +  +        F P S  P  + ++ +  IG   MP  W LG+H CR+ Y + ++V  + RK++E  IP   +W+DIDYM+  + FTFDK  FP  +   L + LH  G   + M+DP I     Y  Y  G++ DVWI+ ADG  F+G VWPG   FPD+    A  +W   + DF+   GVDG+W DMNEPA F                   T T+    +N  R + EL                                       G   H   HN+YG   +  T + +     + RPFVLTR+ F GS +    WTGDN S W +L+ SI+ +L   + G   SG D+ GF  + T +L  RW+  GA +PF R H+     D EP+ + +   E  R+A+  RY ++P+ YTLF  ++  G  V  P+ F  P    L + +   L+G     S   +SP  DE
Sbjct:  165 KVPEDANIYGIGEVTAPFRRTHNVTTLWARD----NPDDFYRNIYGAHPYYQEVR-DGKAHGALLMNAHGMDV-ITTEGRITYKVIGGILDFYFFAPKSGKPNDLSIAYTDLIGKPMMPSHWMLGWHHCRYGYPNIDKVETVKRKYKEANIPLQTVWVDIDYMEETKDFTFDKVNFPQDRMIGLGEQLHKDGQNYVVMVDPAISANTTYEPYVRGTEMDVWIKNADGSDFIGSVWPGFTTFPDWWHPNATKYWNKEIIDFVDMLGVDGLWIDMNEPASFCLGSCGSGKVDAGNQPYRWTYTEEEQAANHTRWEKELKAMGNPPGEERNLLYPKYAINNGAGNLSEFTVATTALHYGNIPHYDIHNLYGHAESHITRQALIKHKNKIRPFVLTRSSFPGSGKSVGHWTGDNHSFWPYLKNSIANILNFQMFGVSYSGADVCGFNSDTTEELCTRWMEIGAFYPFARNHNNNAAKDQEPYLW-ESTAEASRIAINTRYEMLPYFYTLFEESNRLGLGVWRPLIFEYPAYEELVSNDVQTLVG-----SDILLSPVLDE 708