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Publication Overview
Abstract
Reniform nematodes (Rotylenchulus reniformis Linford & Oliveira) decrease U.S. production of Upland cotton (Gossypium hirsutum L., 2n = 52, 2[AD](1)) by more than US$100 million yr(-1). We report here on the mapping of a gene for extreme resistance that was introgressed from the African species G. longicalyx (Hutch. & Lee, 2n = 2x = 26\\; 2F(1)). The responsible allele, designated Ren(Ion), was localized to chromosome 11 by first screening A-subgenome simple sequence repeat (SSR) marker loci for parental polymorphism and then for association with resistance. The three most strongly coupled SSRs and a G. longicalyx gene conferring green seed fuzz, designated Fzg(Ion), were screened against 984 resistant and susceptible individuals of multiple backcross generations. We used marker data and pedigrees to identify nonrecombinant heterozygous parents and thereby avoid bias from repeated sampling of a recombination event. We constructed linkage maps after progeny testing a small population (147) and after implementing three alternative approaches better suited to larger populations-marker-assisted genotyping analysis, applying a cut-off value as population-wide genotyping criterion, and genotype-selective sampling. The maps concordantly indicated the order to be Fzg(Ion)-Ren(Ion)-BNL3279_114-BNL1066_156-BNL836_215, with most Ren-proximal bilaterally flanking markers within 6 cM of each other. The results will clearly facilitate use of Ren(Ion) in breeding, additional mapping, genomics, and prospective cloning.
Image
File Name | Legend |
HLA_CRS-49-1151.jpg | Interspecific breeding scheme used to create the HLA [(G. hirsutum × G. longicalyx) chromosome-doubled × G. armourianum]. [Image from Dighe et al., 2009, CRS-49-1151] |
CRS-49-1151_F4.jpg | Ginned seed from homozygous, heterozygous, and susceptible reniform nematode resistant plant exhibiting dark green, light green, and white fuzz color. [Image from Dighe et al., 2009, CRS-49-1151] |
CRS-49-1151_F3_poly.jpg | Polymorphisms of BNL1066 and BNL3279 on a reniform nematode resistance trait-association panel. [Image from Dighe et al., 2009, CRS-49-1151] |
CRS-49-1151_F2.jpg | Flow diagram describing the development of BC1S1, BC3S1, and BC6S1 populations that were phenotyped for the reniform nematode resistance and genotyped for flanking markers. [Image from Dighe et al., 2009, CRS-49-1151] |
CRS-49-1151_T.jpg | Identities of uni- to multigeneration lineages of Upland cotton (Gossypium hirsutum L.) selected for reniform nematode resistance and linkage mapping. [Image from Dighe et al., 2009, CRS-49-1151] |
CRS-49-1151_F5_poly.jpg | Polymorphisms of BNL1066 and BNL3279 on a reniform nematode resistance DNA panel. [Image from Dighe et al., 2009, CRS-49-1151] |
Properties
Additional
details for this publication include:
Property Name | Value |
Publication Type | Journal Article |
Publication Date | 2009 |
Language Abbr | eng |
Publication Model | [electronic resource]. |
URL | http://dx.doi.org/10.2135/cropsci2008.03.0129 |
Journal Code | CRS |
eISSN | 1435-0653 |
Language | English |
pISSN | 0011-183X |
Publication Code | CRS-49-1151 |
Published Location | United States |
Keywords | cotton, Gossypium, Rotylenchulus reniformis, pest resistance, genetic resistance, introgression, interspecific hybridization, wide hybridization, chromosome mapping, alleles, microsatellite repeats, genetic markers, genetic polymorphism, heterozygosity, linkage (genetics), Gossypium longicalyx, resistance genes, molecular sequence data, incognita race 3; alleles; microsatellite repeats; wide hybridization; chromosome mapping; heterozygosity; linkage (genetics); genetic markers; genetic polymorphism; Gossypium; Rotylenchulus reniformis; Gossypium hirsutum; cotton; introgression; interspecific hybridization; pest resistance; genetic resistance |
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