Fine mapping of the dominant glandless Gene 'Gl 2e' in Sea-island cotton (Gossypium barbadense L.).

Gl 2e is a mutant gene that controls glandless trait in cotton plants and seeds. It is an important gene resource to gossypol-free cottonseed breeding. The objective of this research was to develop SSR markers tightly linked with Gl 2e by using the F2 segregating population containing 1599 plants derived from the cross of G. hirsutum genetic standard line TM-1 and G. barbadense glandless mutant line Hai-1. Genetic analysis suggested that the Gl 2e was an incomplete dominant gene. Based on the backbone of genetic linkage map from G. hirsutum x G. barbadense BC1 published by our laboratory, Gl 2e was located between CIR362 and NAU2251b, NAU3860b, STV033, with a genetic distance 9.27 and 0.96 cM, respectively. This result is useful for cloning Gl 2e gene by map-based cloning method.