Development of Gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the G. anomalum and G. hirsutum genomes

Publication Overview
TitleDevelopment of Gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the G. anomalum and G. hirsutum genomes
AuthorsZhai C, Xu P, Zhang X, Guo Q, Zhang X, Xu Z, Shen X
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Year2015
CitationZhai C, Xu P, Zhang X, Guo Q, Zhang X, Xu Z, Shen X. Development of Gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the G. anomalum and G. hirsutum genomes. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 2015 May 9.

Abstract

KEY MESSAGE
We reported the first development of Gossypium anomalum -derived microsatellite markers and identification of recombination between sexually incompatible species by a synthesized hexaploid on genome level. To continue to develop improved cotton varieties, it is essential to transfer desired characters from diploid wild cotton species such as Gossypium anomalum to cultivated allotetraploid cotton species. However, interspecific reproductive barriers limit gene transfer between species. In a previous study, we used colchicine treatment to produce a synthesized hexaploid derived from an interspecific hybrid between Gossypium hirsutum and G. anomalum and demonstrated its hybridity and doubled status using morphological, cytological and molecular marker methods. In the current study, to effectively monitor G. anomalum genome components in the G. hirsutum background, we developed 5974 non-redundant G. anomalum-derived SSR primer pairs using RNA-Seq technology, which were combined with a publicly available physical map. Based on this combined map and segregation data from the BC2F1 population, we identified a set of 230 informative G. anomalum-specific SSR markers distributed on the chromosomes, which cover 95.72 % of the cotton genome. After analyzing BC2F1 segregation data, 50 recombination types from 357 recombination events were identified, which cover 81.48 % of the corresponding G. anomalum genome. A total of 203 recombination events occurred on chromosome 11, accounting for 56.86 % of the recombination events on all chromosomes. Recombination hotspots were observed at marker intervals JAAS1148-NAU5100 on chromosome 1 and JAAS0426-NAU998 on chromosome 2. Therefore, all G. anomalum chromosomes are capable of recombining with At chromosomes in G. hirsutum. This study represents an important step towards introgressing desirable traits into cultivated cotton from the wild cotton species G. anomalum.

Features
This publication contains information about 6,654 features:
Feature NameUniquenameType
JAAS0826JAAS0826genetic_marker
JAAS0827JAAS0827genetic_marker
JAAS0828JAAS0828genetic_marker
JAAS0829JAAS0829genetic_marker
JAAS0830JAAS0830genetic_marker
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JAAS0832JAAS0832genetic_marker
JAAS0833JAAS0833genetic_marker
JAAS0834JAAS0834genetic_marker
JAAS0835JAAS0835genetic_marker
JAAS0836JAAS0836genetic_marker
JAAS0837JAAS0837genetic_marker
JAAS0838JAAS0838genetic_marker
JAAS0839JAAS0839genetic_marker
JAAS0840JAAS0840genetic_marker
JAAS0841JAAS0841genetic_marker
JAAS0842JAAS0842genetic_marker
JAAS0843JAAS0843genetic_marker
JAAS0844JAAS0844genetic_marker
JAAS0845JAAS0845genetic_marker
JAAS0846JAAS0846genetic_marker
JAAS0847JAAS0847genetic_marker
JAAS0848JAAS0848genetic_marker
JAAS0849JAAS0849genetic_marker
JAAS0850JAAS0850genetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
(86-1 x G. anomalum) x Su8289, BC2F1 (2015)
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1432-2242
eISSN1432-2242
Publication Date2015 May 9
Journal AbbreviationTheor. Appl. Genet.
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article