Development of Gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the G. anomalum and G. hirsutum genomes

Publication Overview
TitleDevelopment of Gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the G. anomalum and G. hirsutum genomes
AuthorsZhai C, Xu P, Zhang X, Guo Q, Zhang X, Xu Z, Shen X
TypeJournal Article
Journal NameTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Year2015
CitationZhai C, Xu P, Zhang X, Guo Q, Zhang X, Xu Z, Shen X. Development of Gossypium anomalum-derived microsatellite markers and their use for genome-wide identification of recombination between the G. anomalum and G. hirsutum genomes. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 2015 May 9.

Abstract

KEY MESSAGE
We reported the first development of Gossypium anomalum -derived microsatellite markers and identification of recombination between sexually incompatible species by a synthesized hexaploid on genome level. To continue to develop improved cotton varieties, it is essential to transfer desired characters from diploid wild cotton species such as Gossypium anomalum to cultivated allotetraploid cotton species. However, interspecific reproductive barriers limit gene transfer between species. In a previous study, we used colchicine treatment to produce a synthesized hexaploid derived from an interspecific hybrid between Gossypium hirsutum and G. anomalum and demonstrated its hybridity and doubled status using morphological, cytological and molecular marker methods. In the current study, to effectively monitor G. anomalum genome components in the G. hirsutum background, we developed 5974 non-redundant G. anomalum-derived SSR primer pairs using RNA-Seq technology, which were combined with a publicly available physical map. Based on this combined map and segregation data from the BC2F1 population, we identified a set of 230 informative G. anomalum-specific SSR markers distributed on the chromosomes, which cover 95.72 % of the cotton genome. After analyzing BC2F1 segregation data, 50 recombination types from 357 recombination events were identified, which cover 81.48 % of the corresponding G. anomalum genome. A total of 203 recombination events occurred on chromosome 11, accounting for 56.86 % of the recombination events on all chromosomes. Recombination hotspots were observed at marker intervals JAAS1148-NAU5100 on chromosome 1 and JAAS0426-NAU998 on chromosome 2. Therefore, all G. anomalum chromosomes are capable of recombining with At chromosomes in G. hirsutum. This study represents an important step towards introgressing desirable traits into cultivated cotton from the wild cotton species G. anomalum.

Features
This publication contains information about 6,654 features:
Feature NameUniquenameType
JAAS6303JAAS6303genetic_marker
JAAS6304JAAS6304genetic_marker
JAAS6305JAAS6305genetic_marker
JAAS6306JAAS6306genetic_marker
JAAS6307JAAS6307genetic_marker
JAAS6308JAAS6308genetic_marker
JAAS6309JAAS6309genetic_marker
JAAS6310JAAS6310genetic_marker
JAAS6311JAAS6311genetic_marker
JAAS6312JAAS6312genetic_marker
JAAS6313JAAS6313genetic_marker
JAAS6314JAAS6314genetic_marker
JAAS6315JAAS6315genetic_marker
JAAS6316JAAS6316genetic_marker
JAAS6317JAAS6317genetic_marker
JAAS6318JAAS6318genetic_marker
JAAS6319JAAS6319genetic_marker
JAAS6320JAAS6320genetic_marker
JAAS6321JAAS6321genetic_marker
JAAS6322JAAS6322genetic_marker
JAAS6323JAAS6323genetic_marker
JAAS6324JAAS6324genetic_marker
JAAS6325JAAS6325genetic_marker
JAAS6326JAAS6326genetic_marker
JAAS6327JAAS6327genetic_marker

Pages

Featuremaps
This publication contains information about 1 maps:
Map Name
(86-1 x G. anomalum) x Su8289, BC2F1 (2015)
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1432-2242
eISSN1432-2242
Publication Date2015 May 9
Journal AbbreviationTheor. Appl. Genet.
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article