Toward to map-based cloning of domiant glandless gene Gl2e in cotton

Working group session: 
Functional Genomics
Presentation type: 
poster
Authors: 
Hu, Yan; zhang, tianzhen; ma, dan; liu, binliang
Presenter: 
Hu, Yan
Correspondent: 
zhang, tianzhen
Abstract: 
The Gossypium species are characterized by the presence of small and darkly pigmented lysigenous glands filled with gossypol. Duplicate recessive genes gl2gl3 and/or another dominant gene Gl2e, a multi-allele of Gl2 can eliminate all glands on the aerial plant parts and seeds and result in low gossypol content. However, the gene conferring glandless phenotype was previously unknown. Based on our precious study, the Gl2e locus was mapped on Chr12 between molecular marker CLR362 and NAU2251b,NAU3860b,STV033,with a genetic distance 9.27 and 0.96 cM . Further, we narrow the target region to 47-kb interval. In the interval, we detected seven candidate genes, and only one has altered expression level between Hai1 and TM-1. Therefore, it is likely the gene at Gl2e locus. Functional validation of the candidate gene through transcriptome profiling and virus induced gene silencing is in progress.