Heat stress treated cotton (G.hirsutum L.) library, LIBEST_027679 (cDNA)

Overview
Library NameHeat stress treated cotton (G.hirsutum L.) library
Unique NameLIBEST_027679
OrganismGossypium hirsutum (Upland Cotton)
TypecDNA
Properties
Property NameValue
Vector typeBAC; plasmid
VectorPCR-TRAP (GenHunter)
Cloning site1: HindIII; 2: HindIII
Tissue typeleaf
Developmental stage77 day old plants with 4-5 squares (squaring stage)
HostE.coli DH5
CommentsDifferential display technique was used to compare expression patterns of heat treated and untreated cotton plants. Differentially expressed bands were excised from polyacrilamide gels and cDNAs were eluted. Extracted cDNAs were reamplified by PCR. PCR products were cloned to PCR-TRAP vector. Two direction sequencing was done using Lgh Primer (CGACAACACCGATAATC)and Rgh Primer (GACGCGAACGAAGCAAC).
Cross References
External references for this cDNA
DatabaseAccession
NCBI BioSampleLIBEST_027679