Cotton susceptible to Meloidogyne incognita, LIBEST_023635 (cDNA)

Overview
Library NameCotton susceptible to Meloidogyne incognita
Unique NameLIBEST_023635
OrganismGossypium hirsutum (Upland Cotton)
TypecDNA
Properties
Property NameValue
Vector typeplasmid
VectorpSPORT 1
Cloning site1: NotI; 2: SalI
Tissue typeRoot
CommentsTotal RNA was extracted from frozen root tissues using CONCERT Large-Scale RNA Isolation (Invitrogen) according to the manufacture's directions. Poly-A mRNA was isolated using an Oligotex mRNA Bath Protocol kit according to manufacturer's manual (QIAGEN). A cDNA library was constructed from four micrograms of poly-A mRNA, measured by absorbance at 260 nm, in the pSPORT 1 vector exploiting the NotI and SalI restrictions sites at the 5' and 3' ends respectively. It was used Superscript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen) according manufacturer's directions.
Cross References
External references for this cDNA
DatabaseAccession
NCBI BioSampleLIBEST_023635