Cotton Root and Hypocotyl tissues infected with Fusarium oxysporum f.sp. vasinfectum (Fov) Lambda ZIPLOX Library (CFUS), LIBEST_013814 (cDNA)

Overview
Library NameCotton Root and Hypocotyl tissues infected with Fusarium oxysporum f.sp. vasinfectum (Fov) Lambda ZIPLOX Library (CFUS)
Unique NameLIBEST_013814
OrganismGossypium hirsutum (Upland Cotton)
TypecDNA
Properties
Property NameValue
Vector typePhage
VectorLambda ZIPLOX
Cloning site1: SalI; 2: NotI
Tissue typeFov infected root and hypocotyls tissues
Developmental stageseedlings, pooled from 5hours to 7 days post infection with Fov.
HostY1090(ZL)
CommentsmRNA was prepared from pooled root and hypocotyl tissues of the cotton cultivar DeltaEMERALD at different stages of Fov infection from 5hours to 7 days post infection. cDNA was synthesised from a NotI-oligodT primer/adapter using the manufacturers protocols (Life Technologies) and then ligated to a SalI adapter to facilitate directional cloning. The cDNA was cloned into the SalI and NotI sites of the Lambda ZIPLOX phage vector (Life Technologies). Constructed by Caitriona Dowd and Helen McFadden.
Cross References
External references for this cDNA
DatabaseAccession
NCBI BioSampleLIBEST_013814