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Properties
| Property Name | Value |
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| Vector type | plasmid |
| Vector | pSPORT 1 |
| Cloning site | 1: NotI; 2: SalI |
| Tissue type | Root |
| Comments | Total RNA was extracted from frozen root tissues using CONCERT Large-Scale RNA Isolation (Invitrogen) according to the manufacture's directions. Poly-A mRNA was isolated using an Oligotex mRNA Bath Protocol kit according to manufacturer's manual (QIAGEN). A cDNA library was constructed from four micrograms of poly-A mRNA, measured by absorbance at 260 nm, in the pSPORT 1 vector exploiting the NotI and SalI restrictions sites at the 5' and 3' ends respectively. It was used Superscript Plasmid System with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen) according manufacturer's directions. |
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