|
|
Properties
| Property Name | Value |
|---|
| Vector type | BAC; plasmid |
| Vector | pTA2 |
| Cloning site | 1: MseI; 2: MseI |
| Tissue type | anther |
| Host | DH5alpha |
| Comments | cDNA-AFLP technique. Gossypium hirsutum plant male sterile and fertile anthers were collected after identification by the microscope in laboratory.RNA was extracted, and double-stranded cDNA was synthesized frome total RNA. The cDNA was digested with MseI and MseI, adapters were ligated to the termini, and two rounds of PCR were performed. On the first round the cDNAs were amplified with primers specific for the adapters. The products of this first round were amplified with primers based on the adapters containing two additional bases at the 3' end. |
|
