|
Overview
Library Name | Cotton Root and Hypocotyl tissues infected with Fusarium oxysporum f.sp. vasinfectum (Fov) Lambda ZIPLOX Library (CFUS) |
Unique Name | LIBEST_013814 |
Organism | Gossypium hirsutum (Upland Cotton) |
Type | cDNA |
SNP Chip Base
Array Name | Cotton Root and Hypocotyl tissues infected with Fusarium oxysporum f.sp. vasinfectum (Fov) Lambda ZIPLOX Library (CFUS) |
Organism | Gossypium hirsutum (Upland Cotton) |
Type | cDNA |
Properties
Property Name | Value |
Vector type | Phage |
Vector | Lambda ZIPLOX |
Cloning site | 1: SalI; 2: NotI |
Tissue type | Fov infected root and hypocotyls tissues |
Developmental stage | seedlings, pooled from 5hours to 7 days post infection with Fov. |
Host | Y1090(ZL) |
Comments | mRNA was prepared from pooled root and hypocotyl tissues of the cotton cultivar DeltaEMERALD at different stages of Fov infection from 5hours to 7 days post infection. cDNA was synthesised from a NotI-oligodT primer/adapter using the manufacturers protocols (Life Technologies) and then ligated to a SalI adapter to facilitate directional cloning. The cDNA was cloned into the SalI and NotI sites of the Lambda ZIPLOX phage vector (Life Technologies). Constructed by Caitriona Dowd and Helen McFadden. |
|