g.barbadense_cottongen_reftransV1_0097430, g.barbadense_cottongen_reftransV1_0097430 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: Y005_SYNY3 (Uncharacterized protein sll0005 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=sll0005 PE=3 SV=1)

HSP 1 Score: 263.077 bits (671), Expect = 1.025e-72
Identity = 149/442 (33.71%), Postives = 241/442 (54.52%), Query Frame = -3

Query: 2490 LPEA--YDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILR-LGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSL 3806
             PE+  Y  + + +++  RP  V  RL+ +        + I    L+           D  +S+    + L+E + +LGPT+IKVGQ+LSTRPD++ P     L+ L DQ+P FP  +A + IEEEL +P    ++ +S EP+AAAS GQVY+G    G  VAVKVQRP+L   +  D+YI+R L L   + + + +SD     DEL   +  E++Y  EA N  +F   +     + VP ++   T +R LTMEW+ G                   K ++ Q +DA      LV  GV+ SL QLLE G  HADPHPGNL   A GR+ +LDFG++  ++   ++ ++ ++VH+VN D+ SL +   ++D ++P T+++ +   L    G   L   + ++ F  +  ++ ++  ++ FR+P YY L++RS+ +LEG ++
Sbjct:   47 FPESWEYHPDLIMEFYRKRPLQVLGRLINILFPLLRFILGIWWEKLRGK---------DPTVSRAK-AIQLRELLTNLGPTYIKVGQALSTRPDLVPPVFLDELTTLQDQLPSFPNEVAYRFIEEELGAPAEEIYAELSPEPIAAASLGQVYKGKLKTGEAVAVKVQRPDLVRRITLDIYIMRSLSLWARRSVKRLRSDLVAITDELASRVFEEMNYYQEAINGEKFAQLYGSLPEIYVPSIYWQYTGRRVLTMEWVEG-------------IKLTNIKAIQAQGIDATH----LVEVGVQCSLRQLLEHGFFHADPHPGNLLAMADGRLAYLDFGMMSTIQPYQRYGLIEAVVHLVNRDFDSLAKDYVKLDFLKPDTDLKPIIPALGQVFGNA-LGASVAELNFKSITDQMSAMMYEFPFRVPAYYALIIRSMVTLEGIAI 460          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: Y1919_SYNY3 (Uncharacterized protein slr1919 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=slr1919 PE=3 SV=1)

HSP 1 Score: 248.054 bits (632), Expect = 1.620e-68
Identity = 154/460 (33.48%), Postives = 236/460 (51.30%), Query Frame = -3

Query: 2484 KPLPEA--------YDAEEVADYFSHRPHVVAFRLLEV---FSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAK-----RKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAK-RRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSLCF 3812
            KPLP          Y+A+ +A+Y+  RP  V +R LEV   F  F +  +  +  G  +  +   ++D             L+E +  LGPTFIKVGQ+LSTRPD++  +  + L +L DQ+PPF   LA +++EE+L   +   +  IS  PVAAAS GQVYR     G +VAVKVQRPNLR  +  D+Y++RLG    QK  +        D  L  DE G  L  E+DY  E  NA +F +     + ++VP ++   + ++ LT+EW+                   G K  +  K+ A      D++  GV + L QLLE G  HADPHPGNL  T  GR+ ++DFG++ ++E   +  + +SIV ++N D+ +L +    +  + P T+I  +   LE+  G   +   + D  F  +      +   Y FR+P  + L++RSL + EG +L  
Sbjct:    5 KPLPPQSELDNIRRYNAQAIANYYRRRPWKVLWRALEVVWSFGFFLTCLLWDQWTGQVEYYKRQRAED-------------LRELLTKLGPTFIKVGQALSTRPDLVRRDFLEELIKLQDQLPPFDNDLAFQLMEEQLGMKVDEAYREISAHPVAAASLGQVYRAMLFSGEEVAVKVQRPNLRPRLSLDLYLMRLG---AQKFGRFLPLNLGHDLTLIVDEFGIKLFEEIDYLNEGRNAEKFAENFHGDAEVKVPCIYWQYSNQKVLTLEWI------------------QGFKLTDTDKIRAAGLDPSDIIRIGVTSGLRQLLEHGFFHADPHPGNLFATLDGRMAYIDFGMMDQLEPGTKETIASSIVQLINKDYLALTEDFIALGFLAPNTDITPIIPALENVFGSA-IGQSVQDFNFKTITDDFSELMYDYPFRVPAKFALIIRSLVTQEGLALSL 429          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: Y1796_ARATH (Uncharacterized aarF domain-containing protein kinase At1g79600, chloroplastic OS=Arabidopsis thaliana GN=At1g79600 PE=2 SV=1)

HSP 1 Score: 245.358 bits (625), Expect = 1.937e-66
Identity = 147/439 (33.49%), Postives = 237/439 (53.99%), Query Frame = -3

Query: 2490 YDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRG-CTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLL--QKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKR-RLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSL 3794
            Y  E +A     +P     R LE+  +    A+++   G+ +        ++++N+ +    + L+     LGPTF+K+GQ LSTRPD+  P+  + L+EL D +P FP   A   IE ELD  + + FS +S EP+AAAS GQVY+      G  VAVKVQRP +   +  D Y++R G+G L  + +    +D     DE    +  EL+Y  EA NA +F   ++  + + VP +F   T ++ LTMEW+                   G+K  E+  ++++  ++LDLVN G++ SL QLLE G  HADPHPGNL  T  G++ FLDFG++    ++ +FA++  +VH+VN D+ ++ +    +  + P  ++  +   L D   +  L   + ++ F  ++  + +V  +Y F +PPYY L+LRSL  LEG +L
Sbjct:   96 YSPELIARKHGSQPFKALRRSLEILGALGGFALKL---GIDQK-----QGNLEKNMKKR--AIELRRIFTRLGPTFVKLGQGLSTRPDLCPPDYLEELAELQDALPTFPDAEAFACIERELDLSLETIFSSVSPEPIAAASLGQVYKAQLRYSGQVVAVKVQRPGIEEAIGLDFYLIR-GVGKLINKYVDFITTDVLTLIDEFACRVYQELNYVQEAQNARRFKKLYADKADVLVPDIFWDYTSRKVLTMEWV------------------EGTKLNEQLAIESQGLKVLDLVNTGIQCSLRQLLEYGFFHADPHPGNLLATPDGKLAFLDFGMMSETPEEARFAIIGHVVHLVNRDYEAMARDYYALKFLSPDVDVTPIIPALRDFFDDA-LNYTVSELNFKTLVDGLGAVFYQYPFNVPPYYALILRSLTVLEGLAL 504          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: Y4139_ARATH (Uncharacterized aarF domain-containing protein kinase At4g31390, chloroplastic OS=Arabidopsis thaliana GN=At4g31390 PE=2 SV=1)

HSP 1 Score: 238.424 bits (607), Expect = 2.721e-64
Identity = 134/377 (35.54%), Postives = 204/377 (54.11%), Query Frame = -3

Query: 2484 LKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCT-LDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRK--SDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKV-DAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSLCF 3602
            L+  + +LGP+FIK GQ L+ RPDII  +    L  L D +PPFP  +A  IIEEEL  P+ + FS IS + +AAAS GQVYR      G DVA+KVQRP +  ++ RD+++ R     L   + +K   +  L  DE G+ L+ ELDYTLEA N   FL+       +++P V+++L   R L MEW+                   G +  + Q + DA   L   +  GV A+L QLLE G+ H DPHPGN+     GRI ++DFG +  + ++++  ++ ++VH VN D+  +    T +  +   T++  +   LE A+ +     G+ D  F  V G+   +   +  R+P  ++LV+RSL + EG  +CF
Sbjct:  170 LRNLLCNLGPSFIKAGQVLANRPDIIREDYMNELCILQDDVPPFPNEVAFNIIEEELGQPLENIFSKISSQTIAAASLGQVYRATLRATGEDVAIKVQRPQIEPIIYRDLFLFRTLASFLNGFSLQKLGCNAELIVDEFGEKLLEELDYTLEARNIEDFLENFKDDPTVKIPGVYKNLCGPRVLVMEWI------------------DGIRCTDPQAIKDAGIDLNGFLTVGVSAALRQLLEFGLFHGDPHPGNIFAMQDGRIAYVDFGNVAVLSQQNKQILIDAVVHAVNEDYGEMANDFTRLGFLAKDTDVSPIVPALE-AIWQNSAGKGLADFNFRSVTGQFNKLVYDFPIRIPERFSLVIRSLLTQEG--ICF 525          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: Y1181_ARATH (Uncharacterized aarF domain-containing protein kinase At1g71810, chloroplastic OS=Arabidopsis thaliana GN=At1g71810 PE=2 SV=1)

HSP 1 Score: 233.802 bits (595), Expect = 1.151e-62
Identity = 135/372 (36.29%), Postives = 197/372 (52.96%), Query Frame = -3

Query: 2490 LKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRG-CTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSL 3602
            L++ ++ LGP ++K+ Q++S+RPD+I P     LS L DQI PF   +A  +IE+EL  PI   FS IS EPVAAAS GQVY+      G  VAVKVQRP +R  +  D  ILR   GL++K  +  SD     DE    L  E+DY  EA N  +F   +     + VPK++   +  + L MEW+ G                        QK++    L  LV  GV  S  QLLE G  HADPHPGN   T  G++ +LDFG++     + +   + + +H+VN D+ +L +    + ++ P      VT  L D   +  +  G+ +I F  +LG +     ++ FR+PPY++LV+RSLA LEG ++
Sbjct:  123 LRKLLVELGPAYVKIAQAVSSRPDLIPPIYLDELSLLQDQITPFSTEVAFNMIEDELGLPIDELFSEISPEPVAAASLGQVYQARLRRSGKVVAVKVQRPGVRAAIALDTLILRYIAGLIKKAGRFNSDLEAVVDEWATSLFKEMDYLNEAQNGIKFRKLYGGIKDVLVPKMYTEYSTSKVLVMEWVEG------------------------QKLNEVNDLY-LVEVGVYCSFNQLLEYGFYHADPHPGNFLRTYDGQLAYLDFGMMGDFRPELRDGFMEACLHLVNRDFKALAKDFVTLGLLPPTAEKSAVTKALTDVFQDA-ISRGVRNISFGDLLGDLGKTMYRFKFRIPPYFSLVIRSLAVLEGIAI 468          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: Y095_SYNY3 (Uncharacterized protein sll0095 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=sll0095 PE=3 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 6.174e-48
Identity = 125/387 (32.30%), Postives = 200/387 (51.68%), Query Frame = -3

Query: 2499 QYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGL-------LQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLV-NKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTN---IRRVTMDLEDALGEVELKDGIPDIK-FSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 3623
            +Y     L + +L LGPTFIK+GQSLSTR DII  E  +A ++L D++PPF    A+ +IE+EL   I   F      P+A+AS GQV+R     G  V VKVQRP L  ++  D  +L   L L        +++A++     +Y  E    L  E+DY  E  NA +F    + +  ++VP+++   T +  LT+E++                   G K  +RQ ++     LDLV   G+ A L QLL  G   +DPHPGN+   + G + F DFG +  ++   +  M+ +   ++  D + +L+AL  M +V P  +   I+R+   L D       +D   DIK F +V  +++++  +  FR+PP  T +L+S+++L+G
Sbjct:   54 RYRRAQWLVQQLLYLGPTFIKIGQSLSTRADIIPAEYIEAFTQLQDRVPPFDSRQAIAVIEQELHGAIDEIFQQFEVTPLASASLGQVHRAVLPTGEAVVVKVQRPGLDSLLNLDFELLHQTLRLAKRWLPGFRRLAQKYEIEAIY-QEFFSLLFLEIDYIHEGKNAERFRQNFADYPRVRVPEIYWQYTTRMVLTLEYLP------------------GIKVDDRQALETAGINLDLVIQTGICAYLKQLLVDGFFQSDPHPGNMAVDSQGDLIFYDFGTMAEVKIIAKDQMVQTFFAVLRKDTNQVLEALIYMGLVEPKGDLSPIKRIINFLLD-----NFRDKPIDIKAFDQVGEEVYAMFQQQPFRLPPQMTFILKSISTLDG 416          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: UBIB_NEIMB (Probable protein kinase UbiB OS=Neisseria meningitidis serogroup B (strain MC58) GN=ubiB PE=3 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 3.226e-44
Identity = 131/413 (31.72%), Postives = 197/413 (47.70%), Query Frame = -3

Query: 2499 RIRMAGLKKSLR------------PGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAK--RKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 3695
            R R+AGL  SL             P SSK     L      + L+  + SLGP FIK GQ LSTRPD+I  + +  L++L D++PPF   L+ + IE+ L   I   ++    EP+A+AS  QV++     G  VAVKV RPNL  V+ +D+ ++R G G ++++    ++  PR    E  K L  ELD   EAANASQ      +   + VPKVF        LT+EWM G   +D+               L+   +D    L  L + GVE   TQ+   G  HAD HPGN+   A  R   LDFG++  +    +  +  + +   N D+  +  A  E   V   T    +   +  A+ E      I  I F  VL +++ V+ +++  + P   L+ ++L ++EG
Sbjct:   16 RYRLAGLCASLMGSGWICALLKMMPQSSK-----LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLSREQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGEQVAVKVLRPNLLPVIEQDLSLMRFGAGWVERLFADGKRLKPREVVAEFDKYLHDELDLMREAANASQLGRNFQNSDMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAK-------------LKADGID----LHKLADYGVEIFFTQVFRDGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATAHIESGWVPADTRAEELEAAVR-AVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQLVLLQKTLLNIEG 405          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: Y1770_SYNY3 (Uncharacterized protein sll1770 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=sll1770 PE=3 SV=1)

HSP 1 Score: 170.244 bits (430), Expect = 2.126e-42
Identity = 116/374 (31.02%), Postives = 180/374 (48.13%), Query Frame = -3

Query: 2499 LKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAK--RKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYT-ASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTN---IRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 3602
            ++E +LSLGPTFIKVGQ  STR D+   E  + LS+L D++P F    A  IIEEEL  PI   +      P+AAAS GQV++     G DV VKVQRP L+ +   D+ IL+      Q   K  R  D     +E  K L  E DY  E  +A  F        +++VP+V+   T  + LT+E++ G                  S Y   +    +R+  +L   G  A L QLL  G  HADPHPGNL  +  +G + F DFG++  +    +  ++ ++  +   +   ++ +L  +  ++   +   IRR    L D      +     +   +++   ++ +A    FR P  +T V+R+ ++LEG
Sbjct:   88 IRENLLSLGPTFIKVGQLFSTRSDLFPAEYVEELSKLQDEVPAFSYEQAAGIIEEELGKPIAKLYRSFDPVPLAAASLGQVHKAQLHTGEDVVVKVQRPGLKKLFTIDLAILKKIAQYFQNHPKWGRGRDWNGIYEECCKILWQETDYLREGRSADTFRRNFRGEDWVKVPRVYWRYTSTQILTLEYLPGIKI---------------SHYDALEAAGLERK--ELAQLGARAYLFQLLNHGFFHADPHPGNLAVSPEAGELIFYDFGMMGEITPDTKNKLMDTLFGVAEKNAERIVNSLVALGALKETEDMGPIRRSVQFLLDNF----MDKPFEEQSITKISDDLYEIAYDQPFRFPATFTFVMRAFSTLEG 440          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: UBIB_NEIMA (Probable protein kinase UbiB OS=Neisseria meningitidis serogroup A / serotype 4A (strain Z2491) GN=ubiB PE=3 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 7.316e-42
Identity = 128/413 (30.99%), Postives = 194/413 (46.97%), Query Frame = -3

Query: 2499 RIRMAGLKKSLR------------PGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAK--RKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 3695
            R R+AGL  SL             P SSK     L      + L+  + SLGP FIK GQ LSTRPD+I  + +  L++L D++PPF   L+ + IE+ L   I   ++    EP+A+AS  QV++     G  VAVKV RPNL  ++ +D+ ++R G   ++++    R+  P     E  K L  ELD   EAANA Q      + + + VPKVF        LT+EWM G    D+               L+   +D    L  L + GVE   TQ+   G  HAD HPGN+   A  R   LDFG++  +    +  +  + +   N D+  +  A  E   V   T    +   +  A+ E      I  I F  VL +++ V+ +++  + P   L+ ++L ++EG
Sbjct:   16 RYRLAGLCASLMGSGWICALLKMMPQSSK-----LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLSREQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQDLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFHNSNMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAK-------------LKADGID----LHKLADYGVEIFFTQVFRDGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATAHIESGWVPADTRAEELEAAVR-AVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQLVLLQKTLLNIEG 405          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy Swiss-Prot
Match: Y889_SYNY3 (Uncharacterized protein slr0889 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) GN=slr0889 PE=3 SV=1)

HSP 1 Score: 161.77 bits (408), Expect = 1.262e-40
Identity = 111/375 (29.60%), Postives = 181/375 (48.27%), Query Frame = -3

Query: 2499 VLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIA-KRKSDPRLYADELGKGLVGELDYTLEAA---NASQFLDAHSHFSFMQ--VPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 3605
            VL + ++ LGP +IK+GQ LSTRPD++ P    AL+ L   +PP P      +++ E   P+G  F  I  EP+AA S GQ++R     G  VA+KV+RP +  +V +D  +++    LL      +  D    ADE  + +  EL++  EAA   N    L   + F   Q  +PKV+  LT ++ L +EW+ G            S         ++   + K+ +  L+ +   A   QL   G  HADPHPGN+ Y A GR+  +D G++ R++ + +  +   ++ IV+ D     Q   E+       N +R+ +D E  L +      + +  FS V+ +   +A     ++P    L  + LA+LEG
Sbjct:   45 VLTKILVELGPFYIKLGQLLSTRPDLLPPRYINALTALQSNVPPLPWSAIEDLLQREFPQPLGETFQEIESEPIAAGSIGQIHRAVLQSGETVAIKVKRPGIDVIVEQDSLLIKDVAELLALTEFGQNYDIVKLADEFTQTVKAELNFDTEAAYTNNLRTNLAKTTWFDPNQLVIPKVYWELTNQKFLVLEWLDGVPILTADLTQPPS---------DKDIAEKKKEITTLLFR---AFFQQLYVDGFFHADPHPGNIFYLADGRLALIDCGMVGRLDPRTRQLLTEMLLAIVDLDAKRCAQLTVELSESVGRVNFQRLEVDYERMLRKY-YDLSLSEFNFSEVVYEFLRIARVNKLKVPACLGLYAKCLANLEG 406          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: A0A0D2RSB2_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G240500 PE=4 SV=1)

HSP 1 Score: 1072.77 bits (2773), Expect = 0.000e+0
Identity = 570/587 (97.10%), Postives = 575/587 (97.96%), Query Frame = -3

Query: 2499 MALTTSLTASMSVTSLRESRVLKTTTTYXXXXXXXXXXXXXXXXXQAFFANFSHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 4259
            MALTTSLTASMSVTSLRESRVLKTTT   R+++  +QQQQPQRRVQAFF NFSHFGDVVRRDMDFLK GVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSPPAQPPLWPQP YPALSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEV DYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLA+KIIEEEL SPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKR LTMEWM GESSTDLLSITTSSSIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG
Sbjct:    1 MALTTSLTASMSVTSLRESRVLKTTT--YRRKQRVKQQQQPQRRVQAFFGNFSHFGDVVRRDMDFLKTGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSPPAQPPLWPQPYYPALSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVTDYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAVKIIEEELGSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRVLTMEWMVGESSTDLLSITTSSSIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 585          

HSP 2 Score: 433.335 bits (1113), Expect = 9.520e-129
Identity = 218/223 (97.76%), Postives = 218/223 (97.76%), Query Frame = -1

Query:  467 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTELHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAISA 1135
            VVLNRKKEFRWERLALFMRVGAT RSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVP SQYSYKLRLAGG QNTELHPS RLS SSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAISA
Sbjct:  623 VVLNRKKEFRWERLALFMRVGATGRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPVSQYSYKLRLAGGQQNTELHPSARLSASSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAISA 845          

HSP 3 Score: 78.1814 bits (191), Expect = 4.158e-11
Identity = 37/37 (100.00%), Postives = 37/37 (100.00%), Query Frame = -2

Query: 1642 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 1752
            GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH
Sbjct:  585 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 621          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: A0A061FUY1_THECC (Kinase superfamily protein isoform 1 OS=Theobroma cacao GN=TCM_043114 PE=4 SV=1)

HSP 1 Score: 990.719 bits (2560), Expect = 0.000e+0
Identity = 509/587 (86.71%), Postives = 553/587 (94.21%), Query Frame = -3

Query: 2499 MALTTSLTASMSVTSLRESRVLKTTTTYXXXXXXXXXXXXXXXXXQAFFANFSHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 4259
            MALTTSLTA++ +T+ R++R +  TTTYR KQR + +QQ+ QR+VQA F NFSHFGD VRRD++FLKKGV+RG EWA+ETFR+PQ KKA+DDVVWLRNLEDP+FSPPAQPP WPQP YP LSG+DLMMADLKALEAYVSY+YYQSKKWSKPLPEAY+AEEV DYFS RPHVVAFRLLEVFSSFASAAIRIRM+G+KKSLRPGS+K IDEN +QYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRP+AMKIIEE+L SP+GSFF+YIS+EPVAAASFGQVYRGCTLDG DVAVKVQRPNLRHVVVRD+YILRLGLGLLQKIAKRK+DPRLYADELGKGLVGELDYTLEAANAS+FLDAHS FSFMQVPKVF+ LTRKR LTMEWM GES TDLLS +TS+ I HGSKYLERQ+VDAKRRLLDLVNKGVEASLTQLLETG+LHADPHPGNLRY ASG+IGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSL++ALTEMDVVRPGTN RR+TMDLEDALGEVE KDGIPD+KFSRVLGKIW+VALKYHFRMPPYYTLVLRSLASLEG
Sbjct:    1 MALTTSLTAAVPLTAFRDNRGVVKTTTYRMKQRQRGKQQEQQRQVQAVFGNFSHFGDAVRRDVEFLKKGVKRGAEWASETFRVPQVKKALDDVVWLRNLEDPHFSPPAQPPPWPQPYYPELSGLDLMMADLKALEAYVSYYYYQSKKWSKPLPEAYNAEEVVDYFSRRPHVVAFRLLEVFSSFASAAIRIRMSGIKKSLRPGSAKGIDENFAQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPMAMKIIEEDLGSPVGSFFTYISKEPVAAASFGQVYRGCTLDGFDVAVKVQRPNLRHVVVRDIYILRLGLGLLQKIAKRKNDPRLYADELGKGLVGELDYTLEAANASEFLDAHSRFSFMQVPKVFKELTRKRILTMEWMVGESPTDLLSGSTSNPINHGSKYLERQRVDAKRRLLDLVNKGVEASLTQLLETGLLHADPHPGNLRYMASGQIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLIEALTEMDVVRPGTNTRRITMDLEDALGEVEFKDGIPDVKFSRVLGKIWTVALKYHFRMPPYYTLVLRSLASLEG 587          

HSP 2 Score: 352.829 bits (904), Expect = 2.431e-100
Identity = 177/222 (79.73%), Postives = 196/222 (88.29%), Query Frame = -1

Query:  470 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTELHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAIS 1135
            VVLN+KKEFRWER+ALF+RVGATR++LQ V ASSGETS+DNLP+ T+GVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRA VSKEAK FRFQLC+IIADIL Q M ++LGQ+VPASQYSY LRLAGG +N EL PS+RL T +  YDYQSLL DRRLK+I  KILNSARKEPALMLRFYW SFV FIAASALAFHRLLISLS A++G   F PKRFA+S
Sbjct:  625 VVLNKKKEFRWERMALFLRVGATRKTLQWVVASSGETSIDNLPNGTNGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAVVSKEAKVFRFQLCRIIADILCQWMFESLGQIVPASQYSYHLRLAGGPENRELGPSSRLFTPT--YDYQSLLKDRRLKVIFFKILNSARKEPALMLRFYWTSFVMFIAASALAFHRLLISLSEAHLGTLPFAPKRFAMS 844          

HSP 3 Score: 72.7886 bits (177), Expect = 1.694e-9
Identity = 34/37 (91.89%), Postives = 35/37 (94.59%), Query Frame = -2

Query: 1642 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 1752
            GLAVAADP FKTFEAAYP+VVRKLLTENSA TRKILH
Sbjct:  587 GLAVAADPGFKTFEAAYPYVVRKLLTENSAATRKILH 623          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: M5W265_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001434mg PE=4 SV=1)

HSP 1 Score: 833.943 bits (2153), Expect = 0.000e+0
Identity = 418/539 (77.55%), Postives = 470/539 (87.20%), Query Frame = -3

Query: 2499 FANFSHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 4115
              +F H G V R+D++FLK+G+  G++WAN+ FRIP+  K +DD+VWLRNLEDPN +PP   P WPQP YP LSG+DL MADLKA EAY  YFYY SK WSKPLPE YD E V DYF  RPHVVAFRLLEVFSSFASAAIRIR +G+KK LRP   + I+EN+SQYNFGMVLKETML+LGPTFIKVGQSLSTRPDIIG EISKALSELHDQIPPFPR +AMKIIEEEL SP+ S FSYIS EP AAASFGQVYRG TLDG +VA+KVQRPNLRH+VVRD+YILRLGLG+LQKIAKRK D RLYADELGKGLVGELDYTLEA+N+S+F++AHS F FM VPK+FQ L+RKR LTMEW+ GES TDLLS++  SSI +GS Y ERQ++DAKRRLLDLV KGVEA L QLLETG+LHADPHPGNLRYT+SG+IGFLDFGLLC+MEKKHQFAMLASIVHIVNGDW+SL+ +LTEMDV+RPGTNIRRVTMDLE  LGEVE +DGIPD+KFSRVLGKIWS+A KYHFRMPPYY+LVLRSLAS EG
Sbjct:   41 LGDFGHLGQVFRKDVEFLKRGIGSGIQWANKAFRIPEVSKTLDDIVWLRNLEDPN-APPLPAPSWPQPSYPELSGVDLFMADLKAFEAYALYFYYLSKVWSKPLPEVYDPESVGDYFRCRPHVVAFRLLEVFSSFASAAIRIRTSGIKKLLRPSLDEGINENVSQYNFGMVLKETMLNLGPTFIKVGQSLSTRPDIIGAEISKALSELHDQIPPFPRDVAMKIIEEELGSPVESLFSYISGEPEAAASFGQVYRGHTLDGFNVAIKVQRPNLRHIVVRDIYILRLGLGILQKIAKRKGDLRLYADELGKGLVGELDYTLEASNSSKFMEAHSSFPFMFVPKIFQQLSRKRVLTMEWIVGESPTDLLSVSAGSSIDNGSTYSERQRLDAKRRLLDLVKKGVEACLVQLLETGLLHADPHPGNLRYTSSGQIGFLDFGLLCQMEKKHQFAMLASIVHIVNGDWASLVNSLTEMDVIRPGTNIRRVTMDLEYELGEVEFRDGIPDVKFSRVLGKIWSIAFKYHFRMPPYYSLVLRSLASFEG 578          

HSP 2 Score: 252.677 bits (644), Expect = 3.169e-66
Identity = 130/221 (58.82%), Postives = 161/221 (72.85%), Query Frame = -1

Query:  473 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTELHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAI 1135
            VV N+KKEF+W+RLALF++VGA R+ L    AS  ++SL  LP R  G  DVA L+LRLLPSK+GVVLRRL+MTADGASLV+A VSK+AK FR Q C +IADILYQ M  A G+ +  ++YS  LRLA  H N +L PS+R    + +YDY+++  DRRLK+I S +LNSARK P LMLRFYW SFV F  A ALA HR L+S S AY+ P SF  K++AI
Sbjct:  616 VVFNKKKEFQWQRLALFLKVGAARKGLI---ASKADSSLGYLPLRDSGAVDVANLVLRLLPSKEGVVLRRLLMTADGASLVQAMVSKKAKFFRQQFCSVIADILYQWMFTAFGRGIATTRYSSDLRLASAHDNRDLEPSSR----TPIYDYRTIFRDRRLKVIFSNVLNSARKNPILMLRFYWTSFVMFTTALALACHRALVSFSEAYLSPISFARKQYAI 829          

HSP 3 Score: 68.9366 bits (167), Expect = 2.726e-8
Identity = 33/37 (89.19%), Postives = 34/37 (91.89%), Query Frame = -2

Query: 1642 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 1752
            GLAVAAD  FKTFEAAYP+VVRKLLTENSA TRKILH
Sbjct:  578 GLAVAADKKFKTFEAAYPYVVRKLLTENSAATRKILH 614          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: A0A067JSF8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_23939 PE=4 SV=1)

HSP 1 Score: 832.787 bits (2150), Expect = 0.000e+0
Identity = 414/539 (76.81%), Postives = 475/539 (88.13%), Query Frame = -3

Query: 2499 FANFSHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 4115
              NFSHF D VR+D  FLKKG+ RG++WANE FRIPQ  K +DDV+WLRNLEDP  +PP +P  WPQ  Y  L+G+DL+MADLKALEAY SYFYY SK WSKPLPE YD ++V+ YFS RPHVVA RLLEVFS+FASA IRIR + ++K LRP S K+++ N+SQY+FG+VLKETML+LGPTFIKVGQSLSTRPDIIG EISKALSELHDQIPPFPR +AMKIIEEEL SP+ SFFS ISEEPVAAASFGQVYRG T DG DVAVKVQRPNLRHVVVRD+YILRLGLGLLQKIAKRK+D RLYADELGKGLVGELDY+LEAANAS+FLDAHS F FM++PKV+ HL+RKR LTMEW+ GES TDLLS+   +++ HG+   E QK++A+R+LLDLV+KGVEASL QLLETG+LHADPHPGNLRYT+SG++GFLDFGLLC+MEKKHQFAMLASIVHIVNGDW+SL+ +L EMDVVRPGT+I +VTM+LED+LGEV+ +DGIPD+KFSRVL KIWSVALKYHFRMPPYYTLVLRSLASLEG
Sbjct:   41 IGNFSHFRDAVRKDFQFLKKGIGRGIDWANEAFRIPQVFKTLDDVLWLRNLEDPK-APPLEPVAWPQTSYTGLTGVDLVMADLKALEAYASYFYYLSKIWSKPLPEVYDPQDVSHYFSCRPHVVALRLLEVFSAFASATIRIRTSRIRKLLRPSSDKELNGNISQYDFGLVLKETMLNLGPTFIKVGQSLSTRPDIIGTEISKALSELHDQIPPFPRTVAMKIIEEELGSPVESFFSCISEEPVAAASFGQVYRGTTPDGCDVAVKVQRPNLRHVVVRDIYILRLGLGLLQKIAKRKNDLRLYADELGKGLVGELDYSLEAANASKFLDAHSSFLFMRIPKVYHHLSRKRVLTMEWVIGESPTDLLSLAAHNTVDHGAASSEGQKIEARRKLLDLVSKGVEASLVQLLETGLLHADPHPGNLRYTSSGQLGFLDFGLLCQMEKKHQFAMLASIVHIVNGDWASLVGSLIEMDVVRPGTSIWQVTMELEDSLGEVQFRDGIPDVKFSRVLSKIWSVALKYHFRMPPYYTLVLRSLASLEG 578          

HSP 2 Score: 228.794 bits (582), Expect = 2.255e-58
Identity = 123/225 (54.67%), Postives = 160/225 (71.11%), Query Frame = -1

Query:  467 VVLNRKKEFRWERLALFMRVGATRRSLQ-LVEASSGETSLDNLPSR-TDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTELHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAISA 1135
            VVLN++KEFRW+RLAL ++VG+TR  L   + A   E       +R + GVFDVA L+L LLPS+DG+VLR+L+MTADG SLV+A VSKEA  FR QLC++IAD+LYQ  V+ LG    A+ Y+ ++RL       +L PS+RLS    +YDYQS++ DRRLK+I  +IL+SARK+P L+L+F W S V  + ASALA HR+L+SLS  YI P SF  KR AISA
Sbjct:  616 VVLNKRKEFRWDRLALLLKVGSTRNVLNGTIIAPKNEIFPGYQANRPSSGVFDVAQLVLMLLPSRDGIVLRKLLMTADGVSLVQAMVSKEAVIFRQQLCRVIADLLYQWTVQTLGLGTKATLYASQVRLTNESDKRDLFPSSRLSMP--IYDYQSIIKDRRLKIIFFRILDSARKDPVLILKFCWTSIVMIVTASALACHRVLVSLSEVYISPLSFARKRVAISA 838          

HSP 3 Score: 75.0998 bits (183), Expect = 3.712e-10
Identity = 35/37 (94.59%), Postives = 37/37 (100.00%), Query Frame = -2

Query: 1642 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 1752
            GLAVAADP+FKTFEAAYPFVV+KLLTENSAETRKILH
Sbjct:  578 GLAVAADPNFKTFEAAYPFVVKKLLTENSAETRKILH 614          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: V4TIE6_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10030711mg PE=4 SV=1)

HSP 1 Score: 824.313 bits (2128), Expect = 0.000e+0
Identity = 414/543 (76.24%), Postives = 473/543 (87.11%), Query Frame = -3

Query: 2487 FANFSHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSLC 4115
              +FSHFGD VR+DM+FLKK + +G+ WAN+TFR+PQ  K +DDV+WLRNLEDP  +   +P  WPQP YP L+G DL+MADLKALEAY +YFY+  K WSKPLPE Y+ ++VADYF+ RPH+V  RLLEV S F SA IRIR + ++K LR    KD D N+SQYNFGM+LKET+L+LGPTFIKVGQSLSTRPDIIG +ISKALSELHDQIPPFPR +AMKIIEEEL SP+ SFFS+ISEEPVAAASFGQVY G TLDGS VAVKVQRPNLRHVVVRD+YILR+GLGLLQKIAKRKSD RLYADELGKGLVGELDYTLEAANAS+F ++HS F F+ VPKVF++L+RKR LTMEWM GES TDL+S++T SS+   S +L+RQK+DAK RLLDLVNKGVEA+L QLLETG+LHADPHPGNLRYT+SG+IGFLDFGLLCRME+KHQFAMLASIVHIVNGDW SL+ +LTEMDVVRPGTN  RVTMDLEDALGEVE KDGIPD+KFSRVLGKIWS+ALKYHFRMPPYYTLVLRSLASLEG ++ 
Sbjct:   34 IGDFSHFGDAVRKDMEFLKKRIGKGIGWANQTFRLPQVSKTLDDVLWLRNLEDPR-AAELEPCDWPQPSYPGLTGADLLMADLKALEAYANYFYHMLKIWSKPLPEVYNPQDVADYFNCRPHIVGLRLLEVGSCFLSAVIRIRTSRIRKFLRSDLEKDFDGNISQYNFGMILKETVLNLGPTFIKVGQSLSTRPDIIGSDISKALSELHDQIPPFPRSIAMKIIEEELGSPVESFFSFISEEPVAAASFGQVYHGSTLDGSIVAVKVQRPNLRHVVVRDIYILRIGLGLLQKIAKRKSDLRLYADELGKGLVGELDYTLEAANASEFQESHSPFPFIHVPKVFRYLSRKRVLTMEWMVGESPTDLISLSTGSSVDGSSTHLDRQKLDAKWRLLDLVNKGVEATLVQLLETGILHADPHPGNLRYTSSGQIGFLDFGLLCRMERKHQFAMLASIVHIVNGDWQSLVHSLTEMDVVRPGTNTLRVTMDLEDALGEVEFKDGIPDVKFSRVLGKIWSIALKYHFRMPPYYTLVLRSLASLEGLAIA 575          

HSP 2 Score: 263.848 bits (673), Expect = 5.857e-70
Identity = 138/225 (61.33%), Postives = 172/225 (76.44%), Query Frame = -1

Query:  461 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTELHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAISA*N 1135
            VV N+KKEF+W+RL+LF+RVGATR+ LQ V A   ET+LD LP+R  GVFD A L+LRLL + DGVVLRRL+MTADGASL+RA VSKEA  FR +LC++IAD LYQ M +ALG+ +P ++ S +LR+AGG    EL PS+ L  S+T+YDYQS L DRRLK+I SKIL+  R++P LMLR  W +FV  + ASALA  R+L+SLS AY+GP    PKRFAISA N
Sbjct:  609 VVFNKKKEFQWQRLSLFLRVGATRKGLQQVIAPKTETTLDYLPNRV-GVFDAANLVLRLLRTNDGVVLRRLLMTADGASLIRAFVSKEASFFRHELCRVIADALYQWMCEALGRGIPVTR-SSQLRVAGGSDKRELEPSSGL--SATIYDYQSFLKDRRLKVIFSKILDPVRRDPVLMLRLCWAAFVMLVKASALACQRMLVSLSEAYLGPV-LAPKRFAISASN 828          

HSP 3 Score: 67.3958 bits (163), Expect = 7.681e-8
Identity = 30/37 (81.08%), Postives = 33/37 (89.19%), Query Frame = -2

Query: 1642 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 1752
            GLA+A DP FKTFEAAYPFV++KLLTENS  TRKILH
Sbjct:  571 GLAIAGDPHFKTFEAAYPFVIQKLLTENSVATRKILH 607          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: I1NHF2_SOYBN (Uncharacterized protein OS=Glycine max PE=4 SV=1)

HSP 1 Score: 812.757 bits (2098), Expect = 0.000e+0
Identity = 410/543 (75.51%), Postives = 471/543 (86.74%), Query Frame = -3

Query: 2487 FANFSHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSLC 4115
            + +FSH   VVR+DM+FLK+G+  GV WANETFRIP+A K +DDVVWLRNLED   SPP   P WPQP YP LSG+DL+M DL+ALEAY SYFYY SK WS+PLP+AYD +EV+ YFS RPHVV  R+LEV  SFA+A I IR +G +K LR    +D+D+  SQYNFGMVLKET+L+LGPTFIKVGQSLSTRPDIIG E+SKALSELHDQIPPFPR +AMKI+EEE   P+ SFFSYISEEP+AAASFGQVY   T DG++VAVKVQRPNL HVVVRD+YILRLGLGLLQKIAKRKSDPRLYADELGKG VGELDYTLEAANAS+FL+ HS F+FM VPKVF HLTRKR LTMEWM GES TDLLS+T  +S+ + S Y ERQK+DAKRRLLDLV+KGVE++L QLLETG+LHADPHPGNLRYT+SG+IGFLDFGLLC+MEK+HQFAMLASI+HIVNGDW+SL++AL +MDVVRPGTNIR VT++LE ALGEVE K+GIPD+KFSRVLGKIW+VALK+HFRMPPYYTLVLRSLASLEG ++ 
Sbjct:   30 WGDFSHLAQVVRKDMEFLKRGIDNGVAWANETFRIPEAAKKIDDVVWLRNLED-PHSPPLPSPSWPQPWYPGLSGVDLLMYDLEALEAYASYFYYLSKLWSRPLPQAYDPQEVSQYFSVRPHVVTLRVLEVLFSFATAMISIRTSGFRKFLRLIPEEDVDDASSQYNFGMVLKETLLNLGPTFIKVGQSLSTRPDIIGVEMSKALSELHDQIPPFPRTVAMKIMEEEFGCPLESFFSYISEEPMAAASFGQVYFARTTDGNNVAVKVQRPNLHHVVVRDIYILRLGLGLLQKIAKRKSDPRLYADELGKGFVGELDYTLEAANASKFLEVHSSFTFMNVPKVFPHLTRKRVLTMEWMVGESPTDLLSVTAGNSVGNVSGYSERQKLDAKRRLLDLVSKGVESTLVQLLETGLLHADPHPGNLRYTSSGQIGFLDFGLLCQMEKRHQFAMLASIIHIVNGDWASLVRALVDMDVVRPGTNIRLVTLELEQALGEVEFKEGIPDVKFSRVLGKIWTVALKHHFRMPPYYTLVLRSLASLEGLAIA 571          

HSP 2 Score: 238.81 bits (608), Expect = 1.144e-61
Identity = 126/224 (56.25%), Postives = 165/224 (73.66%), Query Frame = -1

Query:  470 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTE--LHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAIS 1135
            V+LN++KEF+W+RL+LF+RVGATR++L+LV AS+ ETSLD+  ++     DVAYL+LRLLPSKDGV +RRL+MTADGASL++A VSKE K FR QLCKII D+LYQ M+K  GQ +  +QYS ++ LA G  N E  L P + L T    YDY S+  DRRL++I SK+L SA ++  LMLRF W S +  I AS LA H+L++SLS AY+G     PKR+A+S
Sbjct:  605 VLLNQRKEFQWQRLSLFLRVGATRKALRLV-ASNSETSLDHSTNKATDTIDVAYLVLRLLPSKDGVAIRRLLMTADGASLIKAMVSKEGKFFRQQLCKIIVDLLYQWMIKLFGQGITVTQYS-RVVLANGPSNKESGLSPRSSLPT----YDYNSIFRDRRLRVIFSKVLKSASRDKILMLRFSWASLLIIITASTLACHQLVVSLSEAYLGKIFDAPKRYAVS 822          

HSP 3 Score: 67.0106 bits (162), Expect = 1.180e-7
Identity = 31/37 (83.78%), Postives = 34/37 (91.89%), Query Frame = -2

Query: 1642 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 1752
            GLA+AAD +FKTFEAAYP+VVRKLLTENSA TR ILH
Sbjct:  567 GLAIAADTNFKTFEAAYPYVVRKLLTENSAATRNILH 603          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: D7TMA9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0019g04130 PE=4 SV=1)

HSP 1 Score: 812.757 bits (2098), Expect = 0.000e+0
Identity = 409/539 (75.88%), Postives = 463/539 (85.90%), Query Frame = -3

Query: 2487 SHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSLC 4103
            + FG+VV +DM+FLKK + RGV+WAN   RIPQ  K++D ++WLR  EDP  +    PP WPQP YP LSG+DL MADLKALE Y SYFY+ SK WSKPLPE YD  EVADYF+ RPH+VA RLLEVFSSFA AAIRIR +G+         +DI+ N+S YNFGMVLKETML+LGPTFIKVGQS+STRPDIIGPEISKALS LHDQIPPFPR +AMKIIEEEL SP+ +FF YISEEPVAAASFGQVY G TLDGS+VAVKVQRPNL HVVVRD+YILR+GLGL+QKIAKRKSDPRLYADELGKGL GELDYTLEAANAS+FL+ HS FSF++VPKV +HL+RKR LTMEWM GE+ +DL+S +  +SI H S Y ERQ+ DAKR+LLDLVNKGVEASL QLL+TG+LHADPHPGNLRY  SG+IGFLDFGLLCRMEKKHQFAMLASIVHIVNGDW SL+ ALTEMD++R GTNI+RVTMDLEDALGEVE KDGIPD+KFS+VLGKIWS+ALKYHFRMPPYYTLVLRSLASLEG ++ 
Sbjct:   33 ASFGEVVGKDMEFLKKRIGRGVQWANGALRIPQLSKSLDRLLWLRMTEDP-LAASLPPPSWPQPSYPGLSGVDLFMADLKALETYASYFYHLSKLWSKPLPEVYDPLEVADYFNRRPHIVALRLLEVFSSFAFAAIRIRTSGITMFYGSNMDRDINGNISPYNFGMVLKETMLNLGPTFIKVGQSISTRPDIIGPEISKALSGLHDQIPPFPRDVAMKIIEEELGSPVEAFFRYISEEPVAAASFGQVYWGITLDGSNVAVKVQRPNLHHVVVRDIYILRIGLGLVQKIAKRKSDPRLYADELGKGLSGELDYTLEAANASEFLETHSSFSFIRVPKVLRHLSRKRVLTMEWMVGENPSDLISASAGNSIAHVSGYSERQQTDAKRQLLDLVNKGVEASLVQLLDTGLLHADPHPGNLRYMPSGQIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWPSLVHALTEMDIIRAGTNIQRVTMDLEDALGEVEFKDGIPDVKFSKVLGKIWSIALKYHFRMPPYYTLVLRSLASLEGLAIA 570          

HSP 2 Score: 253.832 bits (647), Expect = 1.178e-66
Identity = 131/223 (58.74%), Postives = 167/223 (74.89%), Query Frame = -1

Query:  467 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTELHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAISA 1135
            VVLNR+KEF+W++L+LF+RVGATR+ LQ + A +GE  L+  P   +G  DVA L+LRLLPSKDGVVLRRL+MTADGASL+R  +SKEA  FR QLCK IAD+LYQRM++ +GQ +  +Q+S + RL  G  N +L  S+   +S+  YDYQS+L DRRLK+I  KILNS R++P L LRF W SF+ F+ ASALA HR+L+SLS  Y+GP S   KR AISA
Sbjct:  604 VVLNRRKEFQWQKLSLFLRVGATRKGLQQLVAPNGEAPLNYSPGGVNGTVDVANLVLRLLPSKDGVVLRRLLMTADGASLIRMMISKEAIFFRQQLCKAIADVLYQRMLEVIGQGIAITQHSSQWRLRSGPNNRDL--SSLSRSSALTYDYQSVLRDRRLKVIFFKILNSVRRDPVLTLRFCWASFIMFMTASALACHRILVSLSEIYLGPVSLPSKRVAISA 824          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: A0A0B2NZZ7_GLYSO (Uncharacterized protein OS=Glycine soja GN=glysoja_000502 PE=4 SV=1)

HSP 1 Score: 811.601 bits (2095), Expect = 0.000e+0
Identity = 409/543 (75.32%), Postives = 471/543 (86.74%), Query Frame = -3

Query: 2487 FANFSHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSLC 4115
            + +FSH   VVR+DM+FLK+G+  GV WANETFRIP+A K +DDVVWLRNLED   SPP   P WPQP YP LSG+DL+M DL+ALEAY SYFYY SK WS+PLP+AYD +EV+ YFS RPHVV  R+LEV  SFA+A I IR +G +K LR    +D+D+  SQYNFGMVLKET+L+LGPTFIKVGQSLSTRPDIIG E+SKALSELHDQIPPFPR +AMKI+EEE   P+ SFFSYISEEP+AAASFGQVY   T DG++VAVKVQRPNL HVVVRD+YILRLGLGLLQKIAKRKSDPRLYADELGKG VGELDYTLEAANAS+FL+ HS F+FM VPKVF HLTRKR LTMEWM GES TDLLS+T  +S+ + S Y ERQK+DAKRRLLDLV+KGVE++L QLLETG+LHADPHPGNLRYT+SG+IGFLDFGLLC+MEK+HQFAMLASI+HIVNGDW+SL++AL +MDVVRPGTNIR VT++LE ALGEVE K+GIPD+KFS+VLGKIW+VALK+HFRMPPYYTLVLRSLASLEG ++ 
Sbjct:   30 WGDFSHLAQVVRKDMEFLKRGIDNGVAWANETFRIPEAAKKIDDVVWLRNLED-PHSPPLPSPSWPQPWYPGLSGVDLLMYDLEALEAYASYFYYLSKLWSRPLPQAYDPQEVSQYFSVRPHVVTLRVLEVLFSFATAMISIRTSGFRKFLRLIPEEDVDDASSQYNFGMVLKETLLNLGPTFIKVGQSLSTRPDIIGVEMSKALSELHDQIPPFPRTVAMKIMEEEFGCPLESFFSYISEEPMAAASFGQVYFARTTDGNNVAVKVQRPNLHHVVVRDIYILRLGLGLLQKIAKRKSDPRLYADELGKGFVGELDYTLEAANASKFLEVHSSFTFMNVPKVFPHLTRKRVLTMEWMVGESPTDLLSVTAGNSVGNVSGYSERQKLDAKRRLLDLVSKGVESTLVQLLETGLLHADPHPGNLRYTSSGQIGFLDFGLLCQMEKRHQFAMLASIIHIVNGDWASLVRALVDMDVVRPGTNIRLVTLELEQALGEVEFKEGIPDVKFSKVLGKIWTVALKHHFRMPPYYTLVLRSLASLEGLAIA 571          

HSP 2 Score: 238.81 bits (608), Expect = 1.155e-61
Identity = 126/224 (56.25%), Postives = 165/224 (73.66%), Query Frame = -1

Query:  470 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTE--LHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAIS 1135
            V+LN++KEF+W+RL+LF+RVGATR++L+LV AS+ ETSLD+  ++     DVAYL+LRLLPSKDGV +RRL+MTADGASL++A VSKE K FR QLCKII D+LYQ M+K  GQ +  +QYS ++ LA G  N E  L P + L T    YDY S+  DRRL++I SK+L SA ++  LMLRF W S +  I AS LA H+L++SLS AY+G     PKR+A+S
Sbjct:  605 VLLNQRKEFQWQRLSLFLRVGATRKALRLV-ASNSETSLDHSTNKATDTIDVAYLVLRLLPSKDGVAIRRLLMTADGASLIKAMVSKEGKFFRQQLCKIIVDLLYQWMIKLFGQGITVTQYS-RVVLANGPSNKESGLSPRSSLPT----YDYNSIFRDRRLRVIFSKVLKSASRDKILMLRFSWASLLIIITASTLACHQLVVSLSEAYLGKIFDAPKRYAVS 822          

HSP 3 Score: 67.0106 bits (162), Expect = 1.180e-7
Identity = 31/37 (83.78%), Postives = 34/37 (91.89%), Query Frame = -2

Query: 1642 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 1752
            GLA+AAD +FKTFEAAYP+VVRKLLTENSA TR ILH
Sbjct:  567 GLAIAADTNFKTFEAAYPYVVRKLLTENSAATRNILH 603          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: V7BD07_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_007G093900g PE=4 SV=1)

HSP 1 Score: 805.438 bits (2079), Expect = 0.000e+0
Identity = 409/543 (75.32%), Postives = 470/543 (86.56%), Query Frame = -3

Query: 2487 FANFSHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEGKSLC 4115
            + +FSHF  VVR+D++FLK+G+  GV WAN TFRIPQ  K +D+VVWLR+LED   SPP+  P WPQP YP L+ +DL+M DLKALEAY SYFYY SK WSKPLPE YD E+VA YFS RPHVV FR+LEV  S A+A I IR +G KK LR    +D+D+  SQYNFGMVLKET+L+LGPTFIKVGQSLSTRPDIIG E+SKALSELHDQIPPFPR +AMKI+EEE   P+ +FFSYISEEP+AAASFGQVY   T DG++VAVKVQRPNL HVVVRD+YILRLGLGLLQKIAKRKSDPRLYADELGKG VGELDY LEAANAS+F + HS F+FMQVPKVF HLTRKR LTMEWM GES TDLLS+T  +S+ + S+Y ERQK+DAKRRLLDLV+KGVE++L QLLETG+LHADPHPGNLRYT+SG+IGFLDFGLLC+MEK+HQFAMLASIVHIVNGDW+SL++AL +MDVVRPGTNIR VT++LE ALGEVELK+GIPD+KFSRVLGKIW+VALK+HFRMPPYYTLVLRSLASLEG ++ 
Sbjct:   33 WGDFSHFAQVVRKDVEFLKRGIDDGVAWANHTFRIPQVAKKIDEVVWLRHLED-PHSPPSPSPSWPQPWYPGLTAVDLLMYDLKALEAYASYFYYLSKVWSKPLPEVYDPEDVAQYFSVRPHVVTFRVLEVLFSLATAMISIRTSGFKKFLRLVPQEDLDDTSSQYNFGMVLKETLLNLGPTFIKVGQSLSTRPDIIGVEMSKALSELHDQIPPFPRNVAMKIMEEEFGCPLETFFSYISEEPIAAASFGQVYFARTTDGNNVAVKVQRPNLHHVVVRDIYILRLGLGLLQKIAKRKSDPRLYADELGKGFVGELDYKLEAANASKFQEVHSSFTFMQVPKVFPHLTRKRVLTMEWMVGESPTDLLSVTAGNSVGNVSEYSERQKLDAKRRLLDLVSKGVESTLVQLLETGLLHADPHPGNLRYTSSGQIGFLDFGLLCQMEKRHQFAMLASIVHIVNGDWASLVRALIDMDVVRPGTNIRLVTLELEHALGEVELKEGIPDVKFSRVLGKIWTVALKHHFRMPPYYTLVLRSLASLEGLAIA 574          

HSP 2 Score: 228.794 bits (582), Expect = 2.034e-58
Identity = 122/225 (54.22%), Postives = 163/225 (72.44%), Query Frame = -1

Query:  467 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTE--LHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAISA 1135
            V+LNR+KEF+W+RL+LF+RVGATR++L+LV AS+ ET LD+L ++     DVAYL+LRLLPSKDGV +RRL+MTADGASL++A VSKE K+FR QL KI+ D++YQ M+K LG+ +   QYS ++ LA G  N E  L P + L T     DY  +  DRRL++I  KIL SA ++  LMLRF+W S +  + AS LA HRL++SLS AY+      PKR+A+SA
Sbjct:  608 VLLNRRKEFQWQRLSLFLRVGATRKALRLV-ASNSETPLDHLSNKATDTIDVAYLVLRLLPSKDGVAIRRLLMTADGASLIKAVVSKEGKSFRQQLGKIMVDVVYQWMIKLLGEGITVIQYS-RVILANGLSNKESGLSPRSSLPTD----DYNFIFRDRRLRVIFYKILKSASRDKILMLRFFWASLLIMVTASTLACHRLVVSLSEAYLAKIFDAPKRYAVSA 826          

HSP 3 Score: 68.5514 bits (166), Expect = 3.168e-8
Identity = 32/37 (86.49%), Postives = 35/37 (94.59%), Query Frame = -2

Query: 1642 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 1752
            GLA+AAD +FKTFEAAYP+VVRKLLTENSA TRKILH
Sbjct:  570 GLAIAADTNFKTFEAAYPYVVRKLLTENSAATRKILH 606          

BLAST of g.barbadense_cottongen_reftransV1_0097430 vs. ExPASy TrEMBL
Match: A0A059BGQ3_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_G02820 PE=4 SV=1)

HSP 1 Score: 802.742 bits (2072), Expect = 0.000e+0
Identity = 402/539 (74.58%), Postives = 465/539 (86.27%), Query Frame = -3

Query: 2499 FANFSHFGDVVRRDMDFLKKGVQRGVEWANETFRIPQAKKAVDDVVWLRNLEDPNFSXXXXXXXXXXXXXXXLSGMDLMMADLKALEAYVSYFYYQSKKWSKPLPEAYDAEEVADYFSHRPHVVAFRLLEVFSSFASAAIRIRMAGLKKSLRPGSSKDIDENLSQYNFGMVLKETMLSLGPTFIKVGQSLSTRPDIIGPEISKALSELHDQIPPFPRPLAMKIIEEELDSPIGSFFSYISEEPVAAASFGQVYRGCTLDGSDVAVKVQRPNLRHVVVRDVYILRLGLGLLQKIAKRKSDPRLYADELGKGLVGELDYTLEAANASQFLDAHSHFSFMQVPKVFQHLTRKRALTMEWMAGEXXXXXXXXXXXXXIKHGSKYLERQKVDAKRRLLDLVNKGVEASLTQLLETGMLHADPHPGNLRYTASGRIGFLDFGLLCRMEKKHQFAMLASIVHIVNGDWSSLLQALTEMDVVRPGTNIRRVTMDLEDALGEVELKDGIPDIKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASLEG 4115
            F +FSHF D V++D  FL+K + RG+EWANE  RIP   K +DDVVWLR LE+P+ +PP +   WP+PCYP LSG+DL+MADL+ALEAY++YFY  S+ WS+PLPE Y+ E+V  YFS RPHVVA RLLEVF SFASA ++IR +G+   LR  S KD D N+SQYNFG+ LKETML+LGPTFIKVGQSLSTRPDIIG EISKALSELHDQIPPFPR +AM+ I+EE  SP  S FS+ISE+PVAAASFGQVYRG TLDG DVA+KVQRPNLRHVVVRD+YILRL LGL+QKIAKRKSD RLYADELGKGLVGELDYTLEA+NA++F + HSHF+FM+VPKV +HL+RKR LTMEWM GES TDLL ++TS+++  G  Y E Q  DAKRRLLDLV+KGVE++L QLLETG+LHADPHPGNLRYT++G+IGFLDFGLLC+ME++HQ AMLASIVHIVNGDW+SL+QALTEMDVVRPGTN+ RVTMDLEDALGEVE  DGIPD+KFSRVLGKIWSVALKYHFRMPPYYTLVLRSLAS EG
Sbjct:   47 FGDFSHFADAVKKDASFLRKRIGRGIEWANEALRIPLVAKTLDDVVWLRVLENPD-APPVEDCPWPRPCYPELSGLDLVMADLRALEAYMAYFYCLSRVWSRPLPEVYNVEDVNYYFSCRPHVVALRLLEVFFSFASATVKIRASGIASYLRLNSDKDNDGNISQYNFGLALKETMLNLGPTFIKVGQSLSTRPDIIGSEISKALSELHDQIPPFPRTVAMQTIKEEFGSPAQSIFSHISEDPVAAASFGQVYRGKTLDGLDVAIKVQRPNLRHVVVRDIYILRLALGLVQKIAKRKSDLRLYADELGKGLVGELDYTLEASNAAEFSETHSHFTFMRVPKVLEHLSRKRVLTMEWMVGESPTDLLEVSTSNAMVGGCAYSEEQIFDAKRRLLDLVSKGVESTLVQLLETGLLHADPHPGNLRYTSTGQIGFLDFGLLCQMERRHQLAMLASIVHIVNGDWASLIQALTEMDVVRPGTNLWRVTMDLEDALGEVEFTDGIPDVKFSRVLGKIWSVALKYHFRMPPYYTLVLRSLASFEG 584          

HSP 2 Score: 261.922 bits (668), Expect = 2.855e-69
Identity = 132/222 (59.46%), Postives = 166/222 (74.77%), Query Frame = -1

Query:  470 VVLNRKKEFRWERLALFMRVGATRRSLQLVEASSGETSLDNLPSRTDGVFDVAYLLLRLLPSKDGVVLRRLIMTADGASLVRAAVSKEAKAFRFQLCKIIADILYQRMVKALGQLVPASQYSYKLRLAGGHQNTELHPSTRLSTSSTVYDYQSLLSDRRLKLILSKILNSARKEPALMLRFYWVSFVTFIAASALAFHRLLISLSAAYIGPASFIPKRFAIS 1135
            VV  +KKEFRWERL+LF++VG TR+S+Q   A + + SLD +P+R    FDVA L+LRLLPSK GVVLRRL+MTADGASL+RA VSKE   FR QLC IIA+ILY  M+ A+G     +QY+ +++LA G  N EL PS+  STS  +YDYQS+  DRRL++I SK L SA+ +P LMLRF W S V F+ ASALA HR +++LS AY+GP SF+PKRFAIS
Sbjct:  622 VVFTKKKEFRWERLSLFLKVGVTRKSMQGSRALNDDASLDIIPNRNTSAFDVANLVLRLLPSKQGVVLRRLLMTADGASLIRAMVSKEGGFFRQQLCGIIAEILYHWMLGAIGSSTRVAQYNSRVKLASGTSNYELGPSSGRSTS--IYDYQSIFRDRRLRVIFSKALRSAQSDPVLMLRFCWSSLVIFVTASALACHRAVVNLSEAYLGPLSFVPKRFAIS 841          

HSP 3 Score: 70.4774 bits (171), Expect = 8.078e-9
Identity = 32/37 (86.49%), Postives = 36/37 (97.30%), Query Frame = -2

Query: 1642 GLAVAADPSFKTFEAAYPFVVRKLLTENSAETRKILH 1752
            GLAVAADP+FKTF+AAYP+VVRKLLTEN+A TRKILH
Sbjct:  584 GLAVAADPNFKTFQAAYPYVVRKLLTENTAATRKILH 620