g.barbadense_cottongen_reftransV1_0094142, g.barbadense_cottongen_reftransV1_0094142 (contig) Gossypium barbadense
|
Overview
Analyses
This contig is derived from or has results from the following analyses
Homology
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy Swiss-Prot
Match: PP443_ARATH (Pentatricopeptide repeat-containing protein At5g62370 OS=Arabidopsis thaliana GN=At5g62370 PE=2 SV=1) HSP 1 Score: 808.905 bits (2088), Expect = 0.000e+0 Identity = 433/988 (43.83%), Postives = 625/988 (63.26%), Query Frame = -2
Query: 1167 FFKSRRAVTTSAALPLDPSY--ATISSIPADHFSLCLSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEKPAFNA---LVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCA-SMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAM-----KCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTFRK 4097
FFKSR+A T + + L PS A S+ DH S CLS +L RGLL SAR++ +RV+ SS +S+A DF G++LD S Y LI+KL + G +A +FY+ +IG GI+PDSS+ +S+V CL KL + +EA DR++ S P+ N+ +V LC+Q+RFL+AF F ++ L W L GLC G+L EAI M D + T ++LYKSLFY CK+G EAE+LF ME G++VDK MYT L+ YCK M MA+R+Y RM++ D +NTLIHGF+K+G+ D G V+F+QM+++G+Q +V T+H+MI +YC+EG VD A + +NN S++++ N HCYT LI YK+ + +A + RML+ G+VPDH+ +F L+KM PK +EL A ++L++I NGCG +P ++ N +E K+ L+ EI + + +LA V V+ +ALC Q +A ++KM +LGC PL F+YNS+IKCL Q+ + ED SL+N +Q PD T LI++NE CK + + AF I+D ME+ G++P VAIY+ IIGSL +Q +V EA F +MLESG+ PDEI Y+ MIN ++ NGR+ EA+ L E++ +RP+S +YT LISG VK M +KGC YL+KML+DGL PN VLYT+LI +FL+K +F+F+F L LM N I+ D I YI+L+SG +R+++ +K+ + E +EKL Q L R L S P ++ K FA+++I KVK++ +PNLYL+N II+G+C A R+ +AY+H E MQKEG++PN VT+TILM HI+AG+I+ AI LF N C PD ++Y L+ GLC R L+AL+L+ M K G+ P+K +YE +LQ C S L + A K+ ++M P+ WL+ ILC ++KLREA ++ ++Q G+ TK +K +
Sbjct: 11 FFKSRKATTCALSSELFPSTSAAVFSAASGDHRSRCLSLIVKLGRRGLLDSAREVIRRVIDGSSSISEAALVADFAVDNGIELDSSCYGALIRKLTEMGQPGVAETFYNQRVIGNGIVPDSSVLDSMVFCLVKLRRFDEARAHLDRIIA--SGYAPSRNSSSLVVDELCNQDRFLEAFHCFEQVKERGSGLWLWCCKRLFKGLCGHGHLNEAIGMLDTLCGMTRMPLPVNLYKSLFYCFCKRGCAAEAEALFDHMEVDGYYVDKVMYTCLMKEYCKDNNMTMAMRLYLRMVERSFELDPCIFNTLIHGFMKLGMLDKGRVMFSQMIKKGVQSNVFTYHIMIGSYCKEGNVDYALRLFVNNTGSEDISRNVHCYTNLIFGFYKKGGMDKAVDLLMRMLDNGIVPDHITYFVLLKMLPKCHELKYAMVILQSILDNGCGINPPVIDDLGN--IEVKVESLLGEIARKDANLAAVGLAVVTTALCSQRNYIAALSRIEKMVNLGCTPLPFSYNSVIKCLFQENIIEDLASLVNIIQELDFVPDVDTYLIVVNELCKKNDRDAAFAIIDAMEELGLRPTVAIYSSIIGSLGKQGRVVEAEETFAKMLESGIQPDEIAYMIMINTYARNGRIDEANELVEEVVKHFLRPSSFTYTVLISGFVKMGMMEKGCQYLDKMLEDGLSPNVVLYTALIGHFLKKGDFKFSFTLFGLMGENDIKHDHIAYITLLSGLWRAMARKKKRQVI---VEPGKEKLLQRLIRTKPLV---------SIPSSLGNYGSKSFAMEVIGKVKKS-IIPNLYLHNTIITGYCAAGRLDEAYNHLESMQKEGIVPNLVTYTILMKSHIEAGDIESAIDLFEGTN---CEPDQVMYSTLLKGLCDFKRPLDALALMLEMQKSGINPNKDSYEKLLQCLCYSRLTMEAVKVVKDMAALDIWPRSINHTWLIYILCEEKKLREARALFAIMVQSGRSLLNCTKPGLLKMLNQ 978
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy Swiss-Prot
Match: PP445_ARATH (Pentatricopeptide repeat-containing protein At5g65560 OS=Arabidopsis thaliana GN=At5g65560 PE=2 SV=1) HSP 1 Score: 260.766 bits (665), Expect = 1.509e-70 Identity = 220/813 (27.06%), Postives = 359/813 (44.16%), Query Frame = -2
Query: 1374 RLLTDNSCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRR----GSERAREKLFQLLHRQSLLPKEKNLRVSDSSPE--AMKCF-----ALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQK-EGVLPNQVTFTILMG---------------------GHI--------------KAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEAL-SLLHVMHKRGLIPSKGTYEIILQYFCDS 3668
RLL SC+ V LC + + F+ K+I +GC YN L++ L + G ++E Q++ M E + P ++ Y + G CK G V EA K+ G D YTSLI YC+ + + A +V+ M GCR + Y LIHG D LF +M + P+V T+ V+I + C + A L+ M + PN H YTVLI SL + + +A E +ML GL+P+ + + L+ Y K ++ A V ++E + L +N LI C ++ A ++KM +P + TYNSLI + G F+ A LL+ M +G+ PDQ T +I+ CK +E A D+ D +E +G+ P V +Y +I + KV EA M +ML P+ + + +I+G +G++ EA L +M+ ++PT + T LI L+K D + ++ML G P+A YT+ I + ++ A ++ M N + DL Y SL+ G+ + + ++R G E ++ L+ L + K + S PE AM ++L++K+ + PN Y +I G CE ++ A F+ MQ+ EG+ P+++ F L+ GH+ K GE + +F + G D + +KI+++G+ + L+EA L +VM K G S TY ++++ DS
Sbjct: 146 RLLMIKSCDSVGDALYVLDLCRKMNKDERFELKYKLI-----IGC--YNTLLNSLARFGLVDEMKQVYMEMLE-DKVCPNIYTYNKMVNGYCKLGNVEEANQYVSKIVEAGLDPDFFTYTSLIMGYCQRKDLDSAFKVFNEMPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYTVLIKSLCGSERKSEALNLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQMLEKGLMPNVITYNALINGYCKRGMIEDA-------------------------------VDVVELMESRKLSPNTRTYNELIKGYC-KSNVHKAMGVLNKMLERKVLPDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQWTYTSMIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEKMLSKNCLPNSLTFNALIHGLCADGKLKEATLLEEKMVKIGLQPTVSTDTILIHRLLKDGDFDHAYSRFQQMLSSGTKPDAHTYTTFIQTYCREGRLLDAEDMMAKMRENGVSPDLFTYSSLIKGYGDLGQTNFAFDVLKRMRDTGCEPSQHTFLSLIKH---LLEMKYGKQKGSEPELCAMSNMMEFDTVVELLEKMVEHSVTPNAKSYEKLILGICEVGNLRVAEKVFDHMQRNEGISPSELVFNALLSCCCKLKKHNEAAKVVDDMICVGHLPQLESCKVLICGLYKKGEKERGTSVFQNLLQCGYYEDELAWKIIIDGVGKQG-LVEAFYELFNVMEKNGCKFSSQTYSLLIEGPPDS 914 HSP 2 Score: 223.016 bits (567), Expect = 2.144e-58 Identity = 178/724 (24.59%), Postives = 311/724 (42.96%), Query Frame = -2
Query: 1212 YTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFD----PLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMG-------GHIKAGE-----------IDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLL-HVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKY 3314
Y +L+N + + +VY ML+ P+ YTYN +++G+ K+G + ++++E GL P T+ +I YC+ +D A + N M K N YT LI L RI EA + + +M + P + L+K A ++K + G + +L+ ++ +K L+ ++L+ L + +N LI+ C++ + A ++ MES P TYN LIK + + + A +LN+M + + PD T +I+ C+ GN + A+ +L M DRG+ P Y +I SL + K+V EA +F + + GV+P+ ++Y +I+G+ G+V EA + +M+ P S ++ ALI GL + EKM+ GL P T LI L+ +F+ A+ M + + D Y + + + R E A ++ K+++ P+L+ Y+ +I G+ + + A+D + M+ G P+Q TF L+ G K E D + L KM TP+ Y+ L+ G+C+ L A + H+ G+ PS+ + +L C A K+ ++M+ PQL C L+C L + + V+ +++Q G Y
Sbjct: 186 YNTLLNSLARFGLVDEMKQVYMEMLEDKVCPNIYTYNKMVNGYCKLGNVEEANQYVSKIVEAGLDPDFFTYTSLIMGYCQRKDLDSAFKVFNEMPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYTVLIKSLCGSERKSEALNLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQMLEKGLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCKSNVHK-AMGVLNKMLERKVLPDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQWTYTSMIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEKMLSKNCLPNSLTFNALIHGLCADGKLKEATLLEEKMVKIGLQPTVSTDTILIHRLLKDGDFDHAYSRFQQMLSSGTKPDAHTYTTFIQTYCR---------------------------------------------EGRLLDAEDMMAKMRENGVSPDLFTYSSLIKGYGDLGQTNFAFDVLKRMRDTGCEPSQHTFLSLIKHLLEMKYGKQKGSEPELCAMSNMMEFDTVVELLEKMVEHSVTPNAKSYEKLILGICEVGNLRVAEKVFDHMQRNEGISPSELVFNALLSCCCKLKKHNEAAKVVDDMICVGHLPQLESCKVLICGLYKKGEKERGTSVFQNLLQCGYY 863 HSP 3 Score: 213.772 bits (543), Expect = 2.006e-55 Identity = 219/885 (24.75%), Postives = 367/885 (41.47%), Query Frame = -2
Query: 1596 VTTSAALPLDPSYAT-----ISSIPADHFSLC--LSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLD--------LDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRL--------------LTDNSCEKP----------------------AFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMT--DKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQ-IERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAR--EKLFQLLHRQSLLPKEKNLRV--SDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGG 4076
V++ +L LDP A IS P S+ S LIN G + K+ ++ + V DAL +D D L + Y L+ L + G + Y + + + + P+ N +V CKLG +EEA ++ L C++ A+ L+ LC R +A D F+KM + Y VLI LC EA+ + M E T P +H Y L LC Q +A L G+M +G + Y +LIN YCK ++ A+ V M P++ TYN LI G+ K + VL N+M+E+ + P VVT++ +I CR G D A LL+ M + L P+ YT +I SL K R+ EA + ++ + G+ P+ V++ L+ Y K ++D A ++L+ + C LP S + FN LI LC + A+ +KM +G P + T LI L + G F+ A S +M + G PD T I +C+ G L A D++ +M + G+ P + Y+ +I + + A + RM ++G +P + +L++I + EM + + A+ NM D LEKM++ + PNA Y LI + A ++ D M RN+ I + + +L+S + +++ +E A+ + + + H LP+ ++ +V + K + Q + Q + + + +II G + ++ Y+ F +M+K G + T+++L+ G
Sbjct: 93 VSSLFSLDLDPKTALNFSHWISQNPRYKHSVYSYASLLTLLINNGYVGVVFKIRLLMIKSCDSVGDALYVLDLCRKMNKDERFELKYKLIIGCYNTLLNSLARFGLVDEMKQVYMEMLEDK-VCPNIYTYNKMVNGYCKLGNVEEANQYVSKIVEAGLDPDFFTYTSLIMGYCQRKDLDSAFKVFNEMPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYTVLIKSLCGSERKSEALNLVKEM-EETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQMLEKGLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCKSNVHKAMGVL-NKMLERKVLPDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQWTYTSMIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEKMLSKNC------LPNS-------------------------LTFNALIHGLCADGKLKEATLLEEKMVKIGLQPTVSTDTILIHRLLKDGDFDHAYSRFQQMLSSGTKPDAHTYTTFIQTYCREGRLLDAEDMMAKMRENGVSPDLFTYSSLIKGYGDLGQTNFAFDVLKRMRDTGCEPSQHTFLSLI-------------KHLLEMKYGKQKGSEPELCAM------SNMMEFDTVVELLEKMVEHSVTPNAKSYEKLILGICEVGNLRVAEKVFDHMQRNEGISPSELVFNALLSCCCK----------LKKHNEAAKVVDDMICVGH----LPQLESCKVLICGLYKKGEKERGTSVFQNLLQCGYYEDELAWKIIIDGVGKQGLVEAFYELFNVMEKNGCKFSSQTYSLLIEG 910 HSP 4 Score: 173.711 bits (439), Expect = 1.103e-42 Identity = 141/591 (23.86%), Postives = 234/591 (39.59%), Query Frame = -2
Query: 1335 CYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTF-----------------------------------TILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEM 3002
CY L+ SL + + E ++ Y ML + P+ + K++ Y K + EE Q + ++E L + + LI C++ DSA ++M GC Y LI L ++A L +M+ FP T ++I C A +++ +ME+ G+KP + Y +I SL Q K +A + +MLE G+ P+ I Y +ING+ G + +A + M + P + +Y LI G K N+ K L KML+ ++P+ V Y SLI + F+ A+RL+ LM+ + D Y S++ +S +R E A L ++Q PN+ +Y +I G+C+A ++ +A+ E M + LPN +TF TIL+ +K G+ DHA F +M + G PD Y + C+ RLL+A ++ M + G+ P TY +++ + D AF + + M
Sbjct: 185 CYNTLLNSLARFGLVDEMKQVYMEMLEDKVCPNIYTYNKMVNGYCK---------------------------LGNVEEANQYVSKIVEAGLDPDF----FTYTSLIMGYCQRKDLDSAFKVFNEMPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYTVLIKSLCGSERKSEALNLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQMLEKGLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCKSNV-HKAMGVLNKMLERKVLPDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQWTYTSMIDSLCKS--------------KRVEE-------------------------------ACDLFDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEKMLSKNCLPNSLTFNALIHGLCADGKLKEATLLEEKMVKIGLQPTVSTDTILIHRLLKDGDFDHAYSRFQQMLSSGTKPDAHTYTTFIQTYCREGRLLDAEDMMAKMRENGVSPDLFTYSSLIKGYGDLGQTNFAFDVLKRM 698 HSP 5 Score: 173.711 bits (439), Expect = 1.132e-42 Identity = 120/509 (23.58%), Postives = 228/509 (44.79%), Query Frame = -2
Query: 1173 FNVLISALCEQAQQDS-ASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTF 2696
+N L+++L D +M+ +E C P ++TYN ++ + G E+A ++++ G+ PD T +I +C+ +L+ AF + ++M +G + Y +I L +++ EA +FV+M + P Y +I + R EA L EM I+P H+YT LI L + +K L +ML+ GL+PN + Y +LI+ + ++ E A +V+LM+ ++ + Y L+ G+ +S +H+ A+ ++ K+ + + +P++ YN +I G C + AY LM G++P+Q T+T ++ K+ ++ A LF+ + G P+ ++Y L++G C+A ++ EA +L M + +P+ T+ ++ C A + E+MV P + L+ L A + ++ G P T + FI+T+
Sbjct: 186 YNTLLNSLARFGLVDEMKQVYMEMLEDKVC-PNIYTYNKMVNGYCKLGNVEEANQYVSKIVEAGLDPDFFTYTSLIMGYCQRKDLDSAFKVFNEMPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYTVLIKSLCGSERKSEALNLVKEMEETGIKPNIHTYTVLIDSLCSQCKFEKARELLGQMLEKGLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCKSN-----------------------VHK-----------------------AMGVLNKMLERKVLPDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQWTYTSMIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEKMLSKNCLPNSLTFNALIHGLCADGKLKEATLLEEKMVKIGLQPTVSTDTILIHRLLKDGDFDHAYSRFQQMLSSGTKPDAHTYTTFIQTY 647 HSP 6 Score: 155.992 bits (393), Expect = 4.344e-37 Identity = 113/432 (26.16%), Postives = 201/432 (46.53%), Query Frame = -2
Query: 1167 FDILDQMEDRGMK-------PGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPK--EKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTFRK 2435
F ++D+M+ M+ P + YN ++ + V EA +++E+G+DPD Y ++I G+ + A ++F+EM R +YT LI GL D+ KM DD P YT LI + A LV M+ I+ ++ Y L+ S+ S+ ++ E+ARE L Q+L + L+P N ++ M A+ +++ ++ + PN YN +I G+C+++ + A M + VLP+ VT+ L+ G ++G D A L + MN G PD Y +++ LC++ R+ EA L + ++G+ P+ Y ++ +C + A + E+M+ + P N L+ LC KL+EA ++ + +++ G P T ++ I K
Sbjct: 196 FGLVDEMKQVYMEMLEDKVCPNIYTYNKMVNGYCKLGNVEEANQYVSKIVEAGLDPDFFTYTSLIMGYCQRKDLDSAFKVFNEMPLKGCRRNEVAYTHLIHGLCVARRIDEAMDLFVKMKDDECFPTVRTYTVLIKSLCGSERKSEALNLVKEMEETGIKPNIHTYTVLID----SLCSQCKF-------EKARELLGQMLEK-GLMPNVITYNALINGYCKRGMIEDAVDVVELMESRKLSPNTRTYNELIKGYCKSN-VHKAMGVLNKMLERKVLPDVVTYNSLIDGQCRSGNFDSAYRLLSLMNDRGLVPDQWTYTSMIDSLCKSKRVEEACDLFDSLEQKGVNPNVVMYTALIDGYCKAGKVDEAHLMLEKMLSKNCLPNSLTFNALIHGLCADGKLKEATLLEEKMVKIGLQPTVSTDTILIHRLLK 614
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy Swiss-Prot
Match: PP437_ARATH (Putative pentatricopeptide repeat-containing protein At5g59900 OS=Arabidopsis thaliana GN=At5g59900 PE=3 SV=1) HSP 1 Score: 248.054 bits (632), Expect = 2.177e-66 Identity = 193/759 (25.43%), Postives = 352/759 (46.38%), Query Frame = -2
Query: 1407 FNALVRLLCSQERFLDAFDYFIKMININVNL--GCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAF-----MVLKAIALNGCGFDPLL------LPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKN----LRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGT 3632
F+ L++ R LD F KM+ V+L + L+ GL + + A+++F+ M P +++Y + LC+ + A+ + ME+ G V+ Y LI+ CK +K+ A+ + + +PD TY TL++G K+ F+ G + ++M+ PS ++ + GK++ A L+ ++ ++PN Y LI SL K + EAE ++RM GL P+ V + L+ M+ + +LD A MV + L+ ++ L+ +SA E + E++ L V + L+ C + + + A +M G P ++T+ +L+ L + GL DA L N M + P++ T ++I +C+ G++ AF+ L +M ++G+ P Y +I L + SEA + + + +EI Y +++GF G++ EA + EM+ + Y LI G +K L++M D GL P+ V+YTS+I + +F+ AF + DLM + + Y ++++G ++ G E L + S +P + L + M+ A++L + + + N YN++I GFC R+++A + M +GV P+ +T+T ++ + ++ AI L+N M G PD + Y L++G C A + +A L + M ++GLIP+ T
Sbjct: 159 FDLLIQHYVRSRRVLDGVLVF-KMMITKVSLLPEVRTLSALLHGLVKFRHFGLAMELFNDMVS-VGIRPDVYIYTGVIRSLCELKDLSRAKEMIAHMEATGCDVNIVPYNVLIDGLCKKQKVWEAVGIKKDLAGKDLKPDVVTYCTLVYGLCKVQEFEIGLEMMDEMLCLRFSPSEAAVSSLVEGLRKRGKIEEALNLVKRVVDFGVSPNLFVYNALIDSLCKGRKFHEAELLFDRMGKIGLRPNDVTYSILIDMFCRRGKLDTALSFLGEMVDTGLKLSVYPYNSLINGHCKFGDISAAEG-------FMAEMINKKLEPTVVTYTSLMGGYCSKGKINKALRLYHEMTGKGIAPSIYTFTTLLSGLFRAGLIRDAVKLFNEMAEWNVKPNRVTYNVMIEGYCEEGDMSKAFEFLKEMTEKGIVPDTYSYRPLIHGLCLTGQASEAKVFVDGLHKGNCELNEICYTGLLHGFCREGKLEEALSVCQEMVQRGVDLDLVCYGVLIDGSLKHKDRKLFFGLLKEMHDRGLKPDDVIYTSMIDAKSKTGDFKEAFGIWDLMINEGCVPNEVTYTAVINGLCKA------------GFVNEAEVLCSKMQPVSSVPNQVTYGCFLDILTKGEVDMQ-KAVELHNAILKG-LLANTATYNMLIRGFCRQGRIEEASELITRMIGDGVSPDCITYTTMINELCRRNDVKKAIELWNSMTEKGIRPDRVAYNTLIHGCCVAGEMGKATELRNEMLRQGLIPNNKT 894 HSP 2 Score: 239.195 bits (609), Expect = 1.346e-63 Identity = 179/628 (28.50%), Postives = 288/628 (45.86%), Query Frame = -2
Query: 2046 VSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLT-DNSCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQK----IVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQAT--CLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISN 3908
V +A+ + + L D+ TY L+ L + + + + R P + +S+V L K GK+EEA L R++ S +NAL+ LC +F +A F +M I + Y++LID C++G L+ A+ M + L +++ Y SL G CK G + AE +M ++ YTSL+ YC K+ ALR+Y+ M G P YT+ TL+ G + GL LFN+M E ++P+ VT++VMI YC EG + A L M K + P+ + Y LI L + +EA+ F + + G + + + L+ + + +L+ A V + + G D + V + L+ K L++E+ L V + +I A + A D M + GC+P TY ++I L + G +AE L ++MQ P+Q T C + I +++ A ++ + + +G+ A YN +I RQ ++ EA+ + RM+ GV PD I Y TMIN V +A L++ M IRP +Y LI G K +ML GL+PN + SN
Sbjct: 278 VWEAVGIKKDLAGKDLKPDVVTYCTLVYGLCKVQEFEIGLEMMDEMLCLR-FSPSEAAVSSLVEGLRKRGKIEEALNLVKRVVDFGVSPNLFVYNALIDSLCKGRKFHEAELLFDRMGKIGLRPNDVTYSILIDMFCRRGKLDTALSFLGEMVDTGLKL-SVYPYNSLINGHCKFGDISAAEGFMAEMINKKLEPTVVTYTSLMGGYCSKGKINKALRLYHEMTGKGIAPSIYTFTTLLSGLFRAGLIRDAVKLFNEMAEWNVKPNRVTYNVMIEGYCEEGDMSKAFEFLKEMTEKGIVPDTYSYRPLIHGLCLTGQASEAKVFVDGLHKGNCELNEICYTGLLHGFCREGKLEEALSVCQEMVQRGVDLDLVCYGVLIDGSLKHKDRKLFFGLLKEMHDRGLKPDDVIYTSMIDAKSKTGDFKEAFGIWDLMINEGCVPNEVTYTAVINGLCKAGFVNEAEVLCSKMQPVSSVPNQVTYGCFLDILTK-GEVDMQKAVELHNAIL-KGLLANTATYNMLIRGFCRQGRIEEASELITRMIGDGVSPDCITYTTMINELCRRNDVKKAIELWNSMTEKGIRPDRVAYNTLIHGCCVAGEMGKATELRNEMLRQGLIPNNKTSRTTTSN 901 HSP 3 Score: 220.705 bits (561), Expect = 1.139e-57 Identity = 172/727 (23.66%), Postives = 323/727 (44.43%), Query Frame = -2
Query: 1209 LINVYCKGRKMKMALRVYYRML-KMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGF-YRSISSRKRWF--AMRRGSERAREKLFQ-LLH---RQSLLPKEKNLRVSDSSPEA-----MKCFAL---------------KLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYP 3305
LI Y + R++ + V+ M+ K+ P+ T + L+HG VK F LFN M+ G++P V + +I + C + A ++ +M + N Y VLI L K+ ++ EA + + L PD V + L+ K E +I +++E+L ++ A + L+ L ++ + + A + ++ G P LF YN+LI L + F +AE L +RM G+ P+ T I+I+ C+ G L+ A L +M D G+K V YN +I + +S A M+ ++P + Y +++ G+ + G++ +A RL+HEM G I P+ +++T L+SGL + + +M + + PN V Y +I + ++ + AF + M I D Y L+ G +S + F + +G+ E + LLH R+ L E+ L V + + C+ + L++++ P+ +Y +I + ++A+ ++LM EG +PN+VT+T ++ G KAG ++ A L +KM P+ + Y ++ L + ++ LH +GL+ + TY ++++ FC A ++ M+ P ++ LC + +++A +++S+ ++G P
Sbjct: 162 LIQHYVRSRRVLDGVLVFKMMITKVSLLPEVRTLSALLHGLVKFRHFGLAMELFNDMVSVGIRPDVYIYTGVIRSLCELKDLSRAKEMIAHMEATGCDVNIVPYNVLIDGLCKKQKVWEAVGIKKDLAGKDLKPDVVTYCTLVYGLCKVQEFEIGLE-------------------------------MMDEMLCLRFSPSEAAVSSLVEGLRKRGKIEEALNLVKRVVDFGVSPNLFVYNALIDSLCKGRKFHEAELLFDRMGKIGLRPNDVTYSILIDMFCRRGKLDTALSFLGEMVDTGLKLSVYPYNSLINGHCKFGDISAAEGFMAEMINKKLEPTVVTYTSLMGGYCSKGKINKALRLYHEMTGKGIAPSIYTFTTLLSGLFRAGLIRDAVKLFNEMAEWNVKPNRVTYNVMIEGYCEEGDMSKAFEFLKEMTEKGIVPDTYSYRPLIHGLCLTGQASEAKVFVDGLHKGNCELNEICYTGLLHGFCREGKL--EEALSVCQEMVQRGVDLDLVCYGVLIDGSLKHKDRKLFFGLLKEMHDRGLKPDDVIYTSMIDAKSKTGDFKEAFGIWDLMINEGCVPNEVTYTAVINGLCKAGFVNEAEVLCSKMQPVSSVPNQVTYGCFLDILTKGEVDMQKAVELHNAILKGLLANTATYNMLIRGFCRQGRIEEASELITRMIGDGVSPDCITYTTMINELCRRNDVKKAIELWNSMTEKGIRP 855 HSP 4 Score: 103.605 bits (257), Expect = 7.389e-21 Identity = 91/411 (22.14%), Postives = 173/411 (42.09%), Query Frame = -2
Query: 1173 YNCIIGSLFRQKKVSEATAMFVRMLES-GVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSG------FYRSISSRKRWFAMR-RGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTF 2381
++ +I R ++V + +F M+ + P+ +++G A LF++M+ IRP + YT +I L + + + M G N V Y LI +K++ A + + ++ D++ Y +LV G F + +R SE A L + L ++ + + AL L+++V PNL++YN +I C+ + +A F+ M K G+ PN VT++IL+ + G++D A+ +M G Y L+NG C+ + A + M + L P+ TY ++ +C A +++ EM P +Y LL L +R+A +++ + + P + T ++ I+ +
Sbjct: 159 FDLLIQHYVRSRRVLDGVLVFKMMITKVSLLPEVRTLSALLHGLVKFRHFGLAMELFNDMVSVGIRPDVYIYTGVIRSLCELKDLSRAKEMIAHMEATGCDVNIVPYNVLIDGLCKKQKVWEAVGIKKDLAGKDLKPDVVTYCTLVYGLCKVQEFEIGLEMMDEMLCLRFSPSEAAVSSLVEGLRKRGKIEE-----------------ALNLVKRVVDFGVSPNLFVYNALIDSLCKGRKFHEAELLFDRMGKIGLRPNDVTYSILIDMFCRRGKLDTALSFLGEMVDTGLKLSVYPYNSLINGHCKFGDISAAEGFMAEMINKKLEPTVVTYTSLMGGYCSKGKINKALRLYHEMTGKGIAPSIYTFTTLLSGLFRAGLIRDAVKLFNEMAEWNVKPNRVTYNVMIEGY 552
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy Swiss-Prot
Match: PP442_ARATH (Pentatricopeptide repeat-containing protein At5g61990, mitochondrial OS=Arabidopsis thaliana GN=At5g61990 PE=2 SV=1) HSP 1 Score: 245.358 bits (625), Expect = 2.333e-65 Identity = 227/930 (24.41%), Postives = 374/930 (40.22%), Query Frame = -2
Query: 1332 DHFSLCLSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTV-----DFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEKPAFNALVRLLCSQERFLDAF-DYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQ-MFDLMPE-RTESL------------------PTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAM----RRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRF-MPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIP-----SKGTYEIILQYFCDSYLAVPAFKIFEEMV 4013
D FS + L N G A + +R++ + PV++ S++ +FV G D + +L + G++ A +S + G ++P +L +L D LL N LD F D + M+ NV Y++LI C+ G ++ +F E RT +L P + Y L GLCK + +A+SL +M+S G +D Y+ LI+ KGR A + + M+ G Y Y+ I K G+ + LF+ M+ GL P + +I YCRE V LL M +N+ + + Y ++ + + A + M+ G P+ V++ L+K + + A VLK + G D +N LI L + + D A F+ +M G P FTY + I + F A+ + M+ G+ P++ C +INE+CK G + A M D+G+ Y ++ LF+ KV +A +F M G+ PD Y +INGFS G + +A +F EM+ + P Y L+ G + +K L++M GL PNAV Y ++I + + + AFRL D M + D Y +LV G R ++ +R + ++G + L++ K + +K L + RF PN YN++I C+ ++ A + F MQ ++P +T+T L+ G+ K G +F++ A G PD I+Y +++N + +AL L+ M + + S T +L F A K+ E MV
Sbjct: 98 DSFSF---LALDLCNFGSFEKALSVVERMIERNWPVAEVWSSIVRCSQEFV---GKSDDGVLFGILFDGYIAKGYIEEAVFVFSSSM-GLELVP----------------RLSRCKVLLDALLRWNR-------------------LDLFWDVYKGMVERNVVFDVKTYHMLIIAHCRAGNVQLGKDVLFKTEKEFRTATLNVDGALKLKESMICKGLVPLKYTYDVLIDGLCKIKRLEDAKSLLVEMDSLGVSLDNHTYSLLIDGLLKGRNADAAKGLVHEMVSHGINIKPYMYDCCICVMSKEGVMEKAKALFDGMIASGLIPQAQAYASLIEGYCREKNVRQGYELLVEMKKRNIVISPYTYGTVVKGMCSSGDLDGAYNIVKEMIASGCRPNVVIYTTLIKTFLQNSRFGDAMRVLKEMKEQGIAPDIF-------------------------------CYNSLIIGLSKAKRMDEARSFLVEMVENGLKPNAFTYGAFISGYIEASEFASADKYVKEMRECGVLPNKVLCTGLINEYCKKGKVIEACSAYRSMVDQGILGDAKTYTVLMNGLFKNDKVDDAEEIFREMRGKGIAPDVFSYGVLINGFSKLGNMQKASSIFDEMVEEGLTPNVIIYNMLLGGFCRSGEIEKAKELLDEMSVKGLHPNAVTYCTIIDGYCKSGDLAEAFRLFDEMKLKGLVPDSFVYTTLVDGCCR-LNDVERAITIFGTNKKGCASSTAPFNALINWVFKFGKTE-----------LKTEVLNRLMDGSFDRFGKPNDVTYNIMIDYLCKEGNLEAAKELFHQMQNANLMPTVITYTSLLNGYDKMGRRAEMFPVFDEAIAAGIEPDHIMYSVIINAFLKEGMTTKALVLVDQMFAKNAVDDGCKLSISTCRALLSGFAKVGEMEVAEKVMENMV 942 HSP 2 Score: 220.705 bits (561), Expect = 1.755e-57 Identity = 205/870 (23.56%), Postives = 361/870 (41.49%), Query Frame = -2
Query: 1374 FVTSRGLDL--DLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLE--------------EATILFD---RLLTDNSCE-----KPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQ--KREF--EFAFRLVD-LMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNA-----DGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDS 3881
F +S GL+L LS VL+ L++ L L + Y ++ R ++ D + ++I C+ G ++ AT+ D +L C+ K ++ L+ LC +R DA ++M ++ V+L Y++LIDGL + + A + M ++ ++Y + K+G + +A++LF M + G Y SLI YC+ + ++ + M K YTY T++ G G D + + +M+ G +P+VV + +I + + + A +L M + +AP+ CY LI L K R+ EA F M+ GL P+ + + Y + E A +K + CG P KV LI+ C++ + A M G + TY L+ L + +DAE + M+ +GI PD + ++IN K GN++ A I D+M + G+ P V IYN ++G R ++ +A + M G+ P+ + Y T+I+G+ +G + EA RLF EM + P S YT L+ G + N ++ T G + + +LI+ + K E E RL+D DR D+ + I + L KE NL EA K +L +++ MP + Y +++G+ + R + + F+ G+ P+ + +++++ +K G A+ L ++M A DGC + L++G + + A ++ M + IP T ++ C S
Sbjct: 174 FSSSMGLELVPRLSRCKVLLDALLRWNRLDLFWDVYKG-MVERNVVFDVKTYHMLIIAHCRAGNVQLGKDVLFKTEKEFRTATLNVDGALKLKESMICKGLVPLKYTYDVLIDGLCKIKRLEDAKSLLVEMDSLGVSLDNHTYSLLIDGLLKGRNADAAKGLVHEMVSHGINIKP-YMYDCCICVMSKEGVMEKAKALFDGMIASGLIPQAQAYASLIEGYCREKNVRQGYELLVEMKKRNIVISPYTYGTVVKGMCSSGDLDGAYNIVKEMIASGCRPNVVIYTTLIKTFLQNSRFGDAMRVLKEMKEQGIAPDIFCYNSLIIGLSKAKRMDEARSFLVEMVENGLKPNAFTYGAFISGYIEASEFASADKYVKE--MRECGVLP-----------------------------NKVLCTGLINEYCKKGKVIEACSAYRSMVDQGILGDAKTYTVLMNGLFKNDKVDDAEEIFREMRGKGIAPDVFSYGVLINGFSKLGNMQKASSIFDEMVEEGLTPNVIIYNMLLGGFCRSGEIEKAKELLDEMSVKGLHPNAVTYCTIIDGYCKSGDLAEAFRLFDEMKLKGLVPDSFVYTTLVDGCCRLNDVERAITIFGTN-KKGCASSTAPFNALINWVFKFGKTELKTEVLNRLMDGSFDRFGKPNDVTYNIMI-----------------------------------DYLCKEGNL-------EAAK----ELFHQMQNANLMPTVITYTSLLNGYDKMGRRAEMFPVFDEAIAAGIEPDHIMYSVIINAFLKEGMTTKALVLVDQMFAKNAVDDGCKLSISTCRALLSGFAKVGEMEVAEKVMENMVRLQYIPDSATVIELINESCIS 963 HSP 3 Score: 203.756 bits (517), Expect = 4.600e-52 Identity = 172/746 (23.06%), Postives = 322/746 (43.16%), Query Frame = -2
Query: 1287 LCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRS---ISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFC---DSYLAVPAFKIFEEMVVYSPGPQLYYCNWLL 3506
LC G E+A+ + + M ER + P ++ S+ C Q +V +++ D ++ L + Y ++ A+ V+ + + P L+ ++ D W ++ M+E+ + V T+H++I +CR G V +L T + + A + E M+ GLVP LK +D L+ + + LE +L+E + + L +++LI L + D+A + +M S G + Y+ I +S++G+ E A++L + M A G+ P +I +C+ N+ +++L +M+ R + Y ++ + + A + M+ SG P+ +IY T+I F N R +A R+ EM I P Y +LI GL K D+ ++L +M+++GL PNA Y + IS +++ EF A + V M + + + L++ + + I + + +M K + +L KN +V D A ++ ++++ P+++ Y V+I+GF + MQ A F+ M +EG+ PN + + +L+GG ++GEI+ A L ++M+ G P+ + Y +++G C++ L EA L M +GL+P Y ++ C D A+ F ++ S P NW+
Sbjct: 107 LCNFGSFEKALSVVERMIER--NWPVAEVWSSIVR--CSQEFVGKSD-------------DGVLFGILFDGYIAKGYIEEAVFVFSSSMGLELVPRLSRCKVLLDALLRWNRLDLFWDVYKGMVERNVVFDVKTYHMLIIAHCRAGNVQLGKDVL-------FKTEKEFRTATLN-------VDGALKLKESMICKGLVP------------------------LKYT------YDVLIDGLCKIKRLEDAKSLLVE-MDSLGVSLDNHTYSLLIDGLLKGRNADAAKGLVHEMVSHGINIKPYMYDCCICVMSKEGVMEKAKALFDGMIASGLIPQAQAYASLIEGYCREKNVRQGYELLVEMKKRNIVISPYTYGTVVKGMCSSGDLDGAYNIVKEMIASGCRPNVVIYTTLIKTFLQNSRFGDAMRVLKEMKEQGIAPDIFCYNSLIIGLSKAKRMDEARSFLVEMVENGLKPNAFTYGAFISGYIEASEFASADKYVKEMRECGVLPNKVLCTGLINEYCKKGKVIEACSAYRSMVDQGILGDAKTYTVLMNGLF----KNDKVDD---------AEEIFREMRGKGIAPDVFSYGVLINGFSKLGNMQKASSIFDEMVEEGLTPNVIIYNMLLGGFCRSGEIEKAKELLDEMSVKGLHPNAVTYCTIIDGYCKSGDLAEAFRLFDEMKLKGLVPDSFVYTTLVDGCCRLNDVERAITIFGTNKKGCASSTAPFNALINWVF 777 HSP 4 Score: 177.948 bits (450), Expect = 7.839e-44 Identity = 174/680 (25.59%), Postives = 295/680 (43.38%), Query Frame = -2
Query: 1209 YGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMY-PKGYELDIAFMVLKAIAL------NGCG--FDPLLL-------------PVSANEELEQKI--VILIEEILKSNLHLAK-VAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFAL--KLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQ-LYYCNWLLCILCVQQKLREACIVYDSIIQRGKYP 3164
+ WV ++ EQ L +F + + C G + A ++ MI +N P A ++ ++ ++EF G D VLF L Y KGY + F+ ++ L + C D LL V N + K +++I N+ L K V F D A + M G +PL +TY+ LI L + EDA+SLL M + G+ D T ++I+ K N + A ++ +M G+ +Y+C I + ++ + +A A+F M+ SG+ P Y ++I G+ V + L EM I + ++Y ++ G+ D +++M+ G PN V+YT+LI FLQ F A R++ M I D+ Y SL+ G +S KR + AR L +++ L P S FA K ++++++ +PN L +I+ +C+ ++ +A + M +G+L + T+T+LM G K ++D A +F +M G PD Y +L+NG + + +A S+ M + GL P+ Y ++L FC S A ++ +EM V P + YC ++ C L EA ++D + +G P
Sbjct: 84 FNWVDSQKVTEQKLD----SFSFLALDLCNFGSFEKALSVVERMIERNW-PVAEVWSSIVRC---------SQEFV------GKSDDGVLFGILFDGYIAKGYIEEAVFVFSSSMGLELVPRLSRCKVLLDALLRWNRLDLFWDVYKGMVERNVVFDVKTYHMLIIAHCRAGNVQLGKDVLFKTEKEFRTATLNVDGALKLKESMICKGLVPLKYTYDVLIDGLCKIKRLEDAKSLLVEMDSLGVSLDNHTYSLLIDGLLKGRNADAAKGLVHEMVSHGINIKPYMYDCCICVMSKEGVMEKAKALFDGMIASGLIPQAQAYASLIEGYCREKNVRQGYELLVEMKKRNIVISPYTYGTVVKGMCSSGDLDGAYNIVKEMIASGCRPNVVIYTTLIKTFLQNSRFGDAMRVLKEMKEQGIAPDIFCYNSLIIG----LSKAKRM-------DEARSFLVEMVE-NGLKPNAFTYGAFISGYIEASEFASADKYVKEMRECGVLPNKVLCTGLINEYCKKGKVIEACSAYRSMVDQGILGDAKTYTVLMNGLFKNDKVDDAEEIFREMRGKGIAPDVFSYGVLINGFSKLGNMQKASSIFDEMVEEGLTPNVIIYNMLLGGFCRSGEIEKAKELLDEMSVKGLHPNAVTYCT-IIDGYCKSGDLAEAFRLFDEMKLKGLVP 730 HSP 5 Score: 93.2041 bits (230), Expect = 1.045e-17 Identity = 77/296 (26.01%), Postives = 130/296 (43.92%), Query Frame = -2
Query: 3078 SSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEKPAFNALVRLLCS----------QERFLD-AFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKM-----GCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYC 3917
S + A +D ++ +GL + TY +I +SG L A+ + + + +G++PDS + ++V C+L +E A +F + FNAL+ + R +D +FD F K ++ YN++ID LC++G LE A ++F M + +PT+ Y SL G K G E +F + + G D MY+ +IN + K AL + +M GC+ T L+ GF K+G + + M+ P T +I+ C
Sbjct: 675 SGEIEKAKELLDEMSVKGLHPNAVTYCTIIDGYCKSGDLAEAFRLFDEMKL-KGLVPDSFVYTTLVDGCCRLNDVERAITIFGTNKKGCASSTAPFNALINWVFKFGKTELKTEVLNRLMDGSFDRFGKPNDVT-------YNIMIDYLCKEGNLEAAKELFHQM-QNANLMPTVITYTSLLNGYDKMGRRAEMFPVFDEAIAAGIEPDHIMYSVIINAFLKEGMTTKALVLVDQMFAKNAVDDGCKLSISTCRALLSGFAKVGEMEVAEKVMENMVRLQYIPDSATVIELINESC 961
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy Swiss-Prot
Match: PP217_ARATH (Pentatricopeptide repeat-containing protein At3g06920 OS=Arabidopsis thaliana GN=At3g06920 PE=2 SV=1) HSP 1 Score: 236.113 bits (601), Expect = 1.168e-62 Identity = 191/799 (23.90%), Postives = 335/799 (41.93%), Query Frame = -2
Query: 1167 LEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRW---FAMRRGS----ERAR-------------EKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCF-----ALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTFRK 3488
+ AI+ F RTE Y SL + + + + G+M GF ++ K K++ V M K RP Y TLI F + D LF QM E G +P+V F +I + +EG+VD A LL+ M S +L + Y V I S K ++ A +F+ + GL PD V + ++ + K LD A V + E + K+ A+N +I + D A +++ + G +P + YN ++ CL + G ++A + M+ P+ +T I+I+ C+ G L+ AF++ D M+ G+ P V N ++ L + +K+ EA AMF M PDEI + ++I+G GRV +A +++ +M+ + R S YT+LI + G + M++ P+ L + + + E E + + + + D Y L+ G ++ + + + ++M+ RA K +QLL E + S + + A L ++ K R N+ +Y+ +I GF + R+ +AY E + ++G+ PN T+ L+ +KA EI+ A+ F M CTP+ + Y IL+NGLC+ + +A M K+G+ PS +Y ++ + A +F+ P N ++ L + +A +++ +RG + T + + T K
Sbjct: 78 VNRAIEYFRWYERRTELPHCPESYNSLLLVMARCRNFDALDQILGEMSVAGFGPSVNTCIEMVLGCVKANKLREGYDVVQMMRKFKFRPAFSAYTTLIGAFSAVNHSDMMLTLFQQMQELGYEPTVHLFTTLIRGFAKEGRVDSALSLLDEMKSSSLDADIVLYNVCIDSFGKVGKVDMAWKFFHEIEANGLKPDEVTYTSMIGVLCKANRLDEA-------------------------------VEMFEHLEKNRRVPCTYAYNTMIMGYGSAGKFDEAYSLLERQRAKGSIPSVIAYNCILTCLRKMGKVDEALKVFEEMKKDAA-PNLSTYNILIDMLCRAGKLDTAFELRDSMQKAGLFPNVRTVNIMVDRLCKSQKLDEACAMFEEMDYKVCTPDEITFCSLIDGLGKVGRVDDAYKVYEKMLDSDCRTNSIVYTSLIKNFFNHGRKEDGHKIYKDMINQNCSPDLQLLNTYMDCMFKAGEPEKGRAMFEEIKARRFVPDARSYSILIHGLIKAGFANETYELFYSMKEQGCVLDTRAYNIVIDGFCKCGKVNKAYQLLEEMKTKGFEPTVVTYGSVIDGLAKIDRLDEAYMLFEEAKSKRIELNVVIYSSLIDGFGKVGRIDEAYLILEELMQKGLTPNLYTWNSLLDALVKAEEINEALVCFQSMKELKCTPNQVTYGILINGLCKVRKFNKAFVFWQEMQKQGMKPSTISYTTMISGLAKAGNIAEAGALFDRFKANGGVPDSACYNAMIEGLSNGNRAMDAFSLFEETRRRGLPIHNKTCVVLLDTLHK 844 HSP 2 Score: 201.445 bits (511), Expect = 1.185e-51 Identity = 159/695 (22.88%), Postives = 314/695 (45.18%), Query Frame = -2
Query: 1989 PLDPSYAT-ISSIPA-DHFSLCLSFSEQLINRGLLSSARKLFQRVV---SNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCE-KPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDR 4055
P +Y T I + A +H + L+ +Q+ G + LF ++ + V ALS +D + S LD D+ Y V I + G + +A+ F+ + I G+ PD S++ LCK +L+EA +F+ L + A+N ++ S +F +A+ + YN ++ L + G ++EA+++F+ M + ++ P L Y L LC+ G + A L M+ G F + +++ CK +K+ A ++ M C PD T+ +LI G K+G D + ++ +M++ + + + + +I N+ G+ + + +MI++N +P+ + ++K + +E + VPD + Y + I ++ GF ANE E L + + L A+N++I C+ + + A +++M++ G P + TY S+I L++ ++A L +++ I + +I+ K G ++ A+ IL+++ +G+ P + +N ++ +L + ++++EA F M E P+++ Y +ING + +A + EM ++P++ SYT +ISGL K + ++ +G VP++ Y ++I AF L + R
Sbjct: 166 PAFSAYTTLIGAFSAVNHSDMMLTLFQQMQELGYEPTVH-LFTTLIRGFAKEGRVDSALSLLDEMKSSSLDADIVLYNVCIDSFGKVGKVDMAWKFFHE-IEANGLKPDEVTYTSMIGVLCKANRLDEAVEMFEHLEKNRRVPCTYAYNTMIMGYGSAGKFDEAYSLLERQRAKGSIPSVIAYNCILTCLRKMGKVDEALKVFEEM--KKDAAPNLSTYNILIDMLCRAGKLDTAFELRDSMQKAGLFPNVRTVNIMVDRLCKSQKLDEACAMFEEMDYKVCTPDEITFCSLIDGLGKVGRVDDAYKVYEKMLDSDCRTNSIVYTSLIKNFFNHGRKEDGHKIYKDMINQNCSPDLQLLNTYMDCMFKAGEPEKGRAMFEEIKARRFVPD-----------ARSYSILIHGLI-------KAGF--------ANETYE-----LFYSMKEQGCVLDTRAYNIVIDGFCKCGKVNKAYQLLEEMKTKGFEPTVVTYGSVIDGLAKIDRLDEAYMLFEEAKSKRIELNVVIYSSLIDGFGKVGRIDEAYLILEELMQKGLTPNLYTWNSLLDALVKAEEINEALVCFQSMKELKCTPNQVTYGILINGLCKVRKFNKAFVFWQEMQKQGMKPSTISYTTMISGLAKAGNIAEAGALFDRFKANGGVPDSACYNAMIEGLSNGNRAMDAFSLFEETRR 825 HSP 3 Score: 192.586 bits (488), Expect = 8.435e-49 Identity = 152/681 (22.32%), Postives = 286/681 (42.00%), Query Frame = -2
Query: 1602 YNVLIDGLCQKGYLEEAIQMFDLMPERTESLPT-LHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVV----------------------------------TFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPL----LLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILM 3527
+ LI G ++G ++ A+ + D M ++ SL + LY K G V A F ++E+ G D+ YTS+I V CK ++ A+ ++ + K P +Y YNT+I G+ G FD + L + +G PSV+ T++++I CR GK+D A L ++M L PN +++ L K ++ EA +E M PD + F L+ K +D A+ V + + + C + + L+ N ++ + ++++ N N + + + + + +++++ +P +Y+ LI L + G + L M+ QG D I+I+ CK G + A+ +L++M+ +G +P V Y +I L + ++ EA +F ++ + +IY ++I+GF GR+ EA + E++ + P +++ +L+ LVK ++ + M + PN V Y LI+ + R+F AF M + ++ I Y +++SG L K N+ + + L + K +P+ YN +I G +R DA+ FE ++ G+ + T +L+
Sbjct: 206 FTTLIRGFAKEGRVDSALSLLDEM--KSSSLDADIVLYNVCIDSFGKVGKVDMAWKFFHEIEANGLKPDEVTYTSMIGVLCKANRLDEAVEMFEHLEKNRRVPCTYAYNTMIMGYGSAGKFDEAYSLLERQRAKGSIPSVIAYNCILTCLRKMGKVDEALKVFEEMKKDAAPNLSTYNILIDMLCRAGKLDTAFELRDSMQKAGLFPNVRTVNIMVDRLCKSQKLDEACAMFEEMDYKVCTPDEITFCSLIDGLGKVGRVDDAYKVYEKMLDSDCRTNSIVYTSLIKNFFNHGRKEDGHKIYKDMINQNCSPDLQLLNTYMDCMFKAGEPEKGRAMFEEIKARRFVPDARSYSILIHGLIKAGFANETYELFYSMKEQGCVLDTRAYNIVIDGFCKCGKVNKAYQLLEEMKTKGFEPTVVTYGSVIDGLAKIDRLDEAYMLFEEAKSKRIELNVVIYSSLIDGFGKVGRIDEAYLILEELMQKGLTPNLYTWNSLLDALVKAEEINEALVCFQSMKELKCTPNQVTYGILINGLCKVRKFNKAFVFWQEMQKQGMKPSTISYTTMISG----------------------------------LAKAGNIAEAGA-----------LFDRFKANGGVPDSACYNAMIEGLSNGNRAMDAFSLFEETRRRGLPIHNKTCVVLL 839 HSP 4 Score: 158.688 bits (400), Expect = 6.091e-38 Identity = 128/517 (24.76%), Postives = 231/517 (44.68%), Query Frame = -2
Query: 2436 GLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEKP---AFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELE----QKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPA 3965
G + A K+F+ + +++P +LSTY +LI L ++G L A+ D + G+ P+ N +V LCK KL+EA +F+ + D P F +L+ L R DA+ + KM++ + Y LI G E+ +++ M + S P L L + + K G + ++F +++++ F D Y+ LI+ K ++Y M + GC D+ YN +I GF K G + + L +M +G +P+VVT+ +I + ++D A ML SK + N Y+ LI K RI EA E ++ GL P+ + L+ K E++ A + +++ C + + + N + K + +E+ K + + +++ +IS L + A D+ ++ G +P YN++I+ LS DA SL + +G+ TC+++++ K+ LE A
Sbjct: 357 GKVDEALKVFEEMKKDAAP------------------NLSTYNILIDMLCRAGKLDTAFEL-RDSMQKAGLFPNVRTVNIMVDRLCKSQKLDEACAMFEEM--DYKVCTPDEITFCSLIDGLGKVGRVDDAYKVYEKMLDSDCRTNSIVYTSLIKNFFNHGRKEDGHKIYKDMINQNCS-PDLQLLNTYMDCMFKAGEPEKGRAMFEEIKARRFVPDARSYSILIHGLIKAGFANETYELFYSMKEQGCVLDTRAYNIVIDGFCKCGKVNKAYQLLEEMKTKGFEPTVVTYGSVIDGLAKIDRLDEAYMLFEEAKSKRIELNVVIYSSLIDGFGKVGRIDEAYLILEELMQKGLTPNLYTWNSLLDALVKAEEINEALVCFQSMKELKCTPNQVTYGILINGLCKVRKFNKAFVFWQEMQKQGMKPSTISYTTMISGLAKAGNIAEAGALFDRFKANGGVPDSACYNAMIEGLSNGNRAMDAFSLFEETRRRGLPIHNKTCVVLLDTLHKNDCLEQA 851 HSP 5 Score: 125.561 bits (314), Expect = 9.684e-28 Identity = 101/393 (25.70%), Postives = 177/393 (45.04%), Query Frame = -2
Query: 2745 VTSRGLDLDLST----YAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFD-----RLLTDNSCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESL-PTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDP-----LLLPVSANEELEQKIVI 3878
V + LD D T Y LIK G + Y D +I + PD + N+ + C+ K G+ E+ +F+ R + D + L++ + E ++ F M L YN++IDG C+ G + +A Q+ + M +T+ PT+ Y S+ GL K + EA LF + +S+ ++ +Y+SLI+ + K ++ A + +++ G P+ YT+N+L+ VK + V F M E P+ VT+ ++I+ C+ K + A + M + + P+ YT +I+ L K I EA ++R G VPD + +++ G AF + + G LL + N+ LEQ ++
Sbjct: 469 VYEKMLDSDCRTNSIVYTSLIKNFFNHGRKEDGHKIYKD-MINQNCSPDLQLLNTYMDCMFKAGEPEKGRAMFEEIKARRFVPDARSYSILIHGLIKAGFANE----TYELFYSMKEQGCVLDTRAYNIVIDGFCKCGKVNKAYQLLEEM--KTKGFEPTVVTYGSVIDGLAKIDRLDEAYMLFEEAKSKRIELNVVIYSSLIDGFGKVGRIDEAYLILEELMQKGLTPNLYTWNSLLDALVKAEEINEALVCFQSMKELKCTPNQVTYGILINGLCKVRKFNKAFVFWQEMQKQGMKPSTISYTTMISGLAKAGNIAEAGALFDRFKANGGVPDSACYNAMIEGLSNGNRAMDAFSLFEETRRRGLPIHNKTCVVLLDTLHKNDCLEQAAIV 854
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy Swiss-Prot
Match: PP344_ARATH (Pentatricopeptide repeat-containing protein At4g31850, chloroplastic OS=Arabidopsis thaliana GN=PGR3 PE=1 SV=1) HSP 1 Score: 237.269 bits (604), Expect = 2.043e-62 Identity = 223/940 (23.72%), Postives = 403/940 (42.87%), Query Frame = -2
Query: 1197 GLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEKP---AFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGC----------------------------------------GFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQ-MEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGV--DPDEIIYLTMINGFSNNGRVIEADRLFHEMI-GAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLR-VSDSSPEAMKCF-ALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMH-KRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPT 3866
G L+ +Y LI L++S A Y I+ G P +S+++ L K ++ L + T KP F +R+L + +A++ +M + Y VLID LC L+ A ++F+ M + P Y +L + + + +ME G D +T L++ CK A M G P+ +TYNTLI G +++ D LF M G++P+ T+ V I Y + G A M +K +APN + SL K R EA++ + + + GLVPD V + +MK Y K E+D A +L + NGC ++ LL + N ++++ I L E +++ + FN L LC+ + A + KM +GC+P +FTYN++I L + G ++A ++M+ + ++PD T ++ K +E A+ I+ + + +P + +IGS+ + + A + R++ +G+ D D I+ + +I + V A LF + ++P +Y LI GL++ +M + ++ G +P+ Y L+ + + + + F L M ++ E + I + ++SG ++ + A + + L+ + P + D ++ + + A +L + + PN +YN++I+GF +A A F+ M KEGV P+ T+++L+ G +D + F ++ G PD + Y +++NGL ++ RL EAL L + M RG+ P TY ++ + + A KI+ E+ P ++ N L+ + K A VY +++ G P T
Sbjct: 183 GFVLNAYSYNGLIHLLLKSRFCTEAMEVYRRMIL-EGFRPSLQTYSSLMVGLGKRRDIDSVMGLLKEMETLGL--KPNVYTFTICIRVLGRAGKINEAYEILKRMDDEGCGPDVVTYTVLIDALCTARKLDCAKEVFEKM-KTGRHKPDRVTYITLLDRFSDNRDLDSVKQFWSEMEKDGHVPDVVTFTILVDALCKAGNFGEAFDTLDVMRDQGILPNLHTYNTLICGLLRVHRLDDALELFGNMESLGVKPTAYTYIVFIDYYGKSGDSVSALETFEKMKTKGIAPNIVACNASLYSLAKAGRDREAKQIFYGLKDIGLVPDSVTYNMMMKCYSKVGEIDEAIKLLSEMMENGCEPDVIVVNSLINTLYKADRVDEAWKMFMRMKEMKLKPTVVTYNTLLAGLGKNGKIQEAIE-LFEGMVQKGCPPNTITFNTLFDCLCKNDEVTLALKMLFKMMDMGCVPDVFTYNTIIFGLVKNGQVKEAMCFFHQMK-KLVYPDFVTLCTLLPGVVKASLIEDAYKIITNFLYNCADQPANLFWEDLIGSILAEAGIDNAVSFSERLVANGICRDGDSIL-VPIIRYSCKHNNVSGARTLFEKFTKDLGVQPKLPTYNLLIGGLLEADMIEIAQDVFLQVKSTGCIPDVATYNFLLDAYGKSGKIDELFELYKEMSTHECEANTITHNIVISGLVKA-----------GNVDDALDLYYDLMSDRDFSPTACTYGPLIDGLSKSGRLYEAKQLFEGMLDYGCRPNCAIYNILINGFGKAGEADAACALFKRMVKEGVRPDLKTYSVLVDCLCMVGRVDEGLHYFKELKESGLNPDVVCYNLIINGLGKSHRLEEALVLFNEMKTSRGITPDLYTYNSLILNLGIAGMVEEAGKIYNEIQRAGLEPNVFTFNALIRGYSLSGKPEHAYAVYQTMVTGGFSPNTGT 1104 HSP 2 Score: 232.261 bits (591), Expect = 9.315e-61 Identity = 236/992 (23.79%), Postives = 417/992 (42.04%), Query Frame = -2
Query: 1182 EQLINRGL------LSSARKLFQRVVSNSSPVSDALSTVDFVTS---RGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFD----RLLTDNSCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESL-PTLHLYKSLFYGLCKQ-----------------------------------GWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLV-----DLMDR--NQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQK-VKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLH-VMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFI 3983
+ L+ GL + S +K ++R S VS + + DF S + DLS+ + + L ++S++ ++ + N ++ L GKLEE +FD R++ ++ + + + L + A KM L + YN LI L + + EA++++ M E P+L Y SL GL K+ G + EA + +M+ +G D YT LI+ C RK+ A V+ +M +PD TY TL+ F D +++M + G P VVTF +++ C+ G A L+ M + + PN H Y LI L + +R+ +A E + M + G+ P + + Y K + VSA E E+ + + + N+ VA N + +L + + A ++ +G +P TYN ++KC S+ G ++A LL+ M G PD +IN K ++ A+ + +M++ +KP V YN ++ L + K+ EA +F M++ G P+ I + T+ + N V A ++ +M+ P +Y +I GLVK + + +M + P+ V +L+ ++ E A++++ + D+ N DLI I +G ++S +R A G R + + + R S K+ VS A L +K K P L YN++I G EAD ++ A D F ++ G +P+ T+ L+ + K+G+ID L+ +M+ C + I + I+++GL +A + +AL L + +M R P+ TY ++ S A ++FE M+ Y P N L+ + AC ++ +++ G P T S+ +
Sbjct: 31 DNLVTGGLKFHALKIGSRKKHWRRKSMRCSVVS--MKSSDFSGSMIRKSSKPDLSSSEEVTRGLKSFPDTDSSFSYFKSVAGNLNLVHTTETCNYMLEALRVDGKLEEMAYVFDLMQKRIIKRDT---NTYLTIFKSLSVKGGLKQAPYALRKMREFGFVLNAYSYNGLIHLLLKSRFCTEAMEVYRRMI--LEGFRPSLQTYSSLMVGLGKRRDIDSVMGLLKEMETLGLKPNVYTFTICIRVLGRAGKINEAYEILKRMDDEGCGPDVVTYTVLIDALCTARKLDCAKEVFEKMKTGRHKPDRVTYITLLDRFSDNRDLDSVKQFWSEMEKDGHVPDVVTFTILVDALCKAGNFGEAFDTLDVMRDQGILPNLHTYNTLICGLLRVHRLDDALELFGNMESLGVKPTAYTYIVFIDYYGKSGD-----------------------SVSALETFEK----MKTKGIAPNI----VACNASLYSLAKAGRDREAKQIFYGLKDIGLVPDSVTYNMMMKCYSKVGEIDEAIKLLSEMMENGCEPDVIVVNSLINTLYKADRVDEAWKMFMRMKEMKLKPTVVTYNTLLAGLGKNGKIQEAIELFEGMVQKGCPPNTITFNTLFDCLCKNDEVTLALKMLFKMMDMGCVPDVFTYNTIIFGLVKNGQVKEAMCFFHQM-KKLVYPDFVTLCTLLPGVVKASLIEDAYKIITNFLYNCADQPANLFWEDLIGSILAEAGIDNAVSFSERLVA--NGICRDGDSILVPIIRYSC----KHNNVSG---------ARTLFEKFTKDLGVQPKLPTYNLLIGGLLEADMIEIAQDVFLQVKSTGCIPDVATYNFLLDAYGKSGKIDELFELYKEMSTHECEANTITHNIVISGLVKAGNVDDALDLYYDLMSDRDFSPTACTYGPLIDGLSKSGRLYEAKQLFEGMLDYGCRPNCAIYNILINGFGKAGEADAACALFKRMVKEGVRPDLKTYSVLV 968 HSP 3 Score: 226.098 bits (575), Expect = 9.017e-59 Identity = 205/865 (23.70%), Postives = 377/865 (43.58%), Query Frame = -2
Query: 1401 RVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSC-EKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSL--FYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGC-----GFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQ-QDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLI-IINEHCKHGNLEPAFDILDQM-EDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKK-NMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCF--------ALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHF-ELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYE 3932
RV+ + +++A + + G D+ TY VLI L + L A + GR PD +++ L+ + + D + F LV LC F +AFD M + + YN LI GL + L++A+++F M E PT + Y +YG K G V A F KM+++G + + + K + + A +++Y + +G PDS TYN ++ + K+G D L ++MME G +P V+ + +I+ + +VD A + M L P Y L+ L K +I EA E +E M+ G P+ + F L K E+ +A +L + GC ++ ++ + N ++++ + + +K ++ V L+ + + + +D+ + + + P + LI + + ++A S R+ A GI D + L+ II CKH N+ A + ++ +D G++P + YN +IG L + A +F+++ +G PD Y +++ + +G++ E L+ EM + ++ +ISGLVK N+ D Y + M D P A Y LI + A +L + M + Y L++GF ++ G A LF+ + ++ + P K V + C L +++K++ P++ YN+II+G ++ R+++A F E+ G+ P+ T+ L+ AG ++ A ++N++ G P+ + L+ G + + A ++ M G P+ GTYE
Sbjct: 266 RVLGRAGKINEAYEILKRMDDEGCGPDVVTYTVLIDALCTARKLDCAKEVFEKMKTGRH-KPDRVTYITLLDRFSDNRDLDSVKQFWSEMEKDGHVPDVVTFTILVDALCKAGNFGEAFDTLDVMRDQGILPNLHTYNTLICGLLRVHRLDDALELFGNM-ESLGVKPTAYTYIVFIDYYG--KSGDSVSALETFEKMKTKGIAPNIVACNASLYSLAKAGRDREAKQIFYGLKDIGLVPDSVTYNMMMKCYSKVGEIDEAIKLLSEMMENGCEPDVIVVNSLINTLYKADRVDEAWKMFMRMKEMKLKPTVVTYNTLLAGLGKNGKIQEAIELFEGMVQKGCPPNTITFNTLFDCLCKNDEVTLALKMLFKMMDMGCVPDVFTYNTIIFGLVKNGQVKEAMCFFHQ--MKKLVYPDFVTLCTLLPGVVKASLIEDAYKIITNFLYNCADQPANLFWEDLIGSILAEAGIDNAVSFSERLVANGICRDGDSILVPIIRYSCKHNNVSGARTLFEKFTKDLGVQPKLPTYNLLIGGLLEADMIEIAQDVFLQVKSTGCIPDVATYNFLLDAYGKSGKIDELFELYKEMSTHECEANTITHNIVISGLVKAGNVDDALDLYYDLMSDRDFSPTACTYGPLIDGLSKSGRLYEAKQLFEGMLDYGCRPNCAIYNILINGFGKA------------GEADAACALFKRMVKEGVRPDLKTYSV------LVDCLCMVGRVDEGLHYFKELKESGLNPDVVCYNLIINGLGKSHRLEEALVLFNEMKTSRGITPDLYTYNSLILNLGIAGMVEEAGKIYNEIQRAGLEPNVFTFNALIRGYSLSGKPEHAYAVYQTMVTGGFSPNTGTYE 1106 HSP 4 Score: 176.407 bits (446), Expect = 3.524e-43 Identity = 156/662 (23.56%), Postives = 289/662 (43.66%), Query Frame = -2
Query: 2055 ALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYS-FYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEKPAF--NALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMM---------------------EQGLQPSVVTFHVMISN-YCREG--------KVDC------ASMLLNNMISKNLA--PNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANE-----ELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLES-GVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSL 3899
AL T + + ++G+ ++ + L ++G A FY IG ++PDS N ++ C K+G+++EA L ++ +N CE N+L+ L +R +A+ F++M + + YN L+ GL + G ++EAI++F+ M ++ P + +LF LCK V A + KM G D Y ++I K ++K A+ +++M K+ PD T TL+ G VK L + + + + E G+ +V +++N CR+G + C + L +K+L P Y +LI L + + I A++ + ++ + G +PD + L+ Y K ++D F + K ++ + C + + + + ++ + + + + + + LI L + + A + M GC P YN LI + G + A +L RM +G+ PD T ++++ C G ++ ++++ G+ P V YN II L + ++ EA +F M S G+ PD Y ++I G V EA ++++E+ A + P ++ ALI G + + M+ G PN Y L
Sbjct: 452 ALETFEKMKTKGIAPNIVACNASLYSLAKAGRDREAKQIFYGLKDIG--LVPDSVTYNMMMKCYSKVGEIDEAIKLLSEMM-ENGCEPDVIVVNSLINTLYKADRVDEAWKMFMRMKEMKLKPTVVTYNTLLAGLGKNGKIQEAIELFEGMVQKG-CPPNTITFNTLFDCLCKNDEVTLALKMLFKMMDMGCVPDVFTYNTIIFGLVKNGQVKEAMCFFHQMKKL-VYPDFVTLCTLLPGVVKASLIEDAYKIITNFLYNCADQPANLFWEDLIGSILAEAGIDNAVSFSERLVANGICRDGDSILVPIIRYSCKHNNVSGARTLFEKFTKDLGVQPKLPTYNLLIGGLLEADMIEIAQDVFLQVKSTGCIPDVATYNFLLDAYGKSGKIDELFELYKEMSTHECEANTITHNIVISGLVKAGNVDDALDLYYDLMSDRDFSPTACTYGPLIDGLSKSGRLYEAKQLFEGMLDYGCRPNCAIYNILINGFGKAGEADAACALFKRMVKEGVRPDLKTYSVLVDCLCMVGRVDEGLHYFKELKESGLNPDVVCYNLIINGLGKSHRLEEALVLFNEMKTSRGITPDLYTYNSLILNLGIAGMVEEAGKIYNEIQRAGLEPNVFTFNALIRGYSLSGKPEHAYAVYQTMVTGGFSPNTGTYEQL 1108 HSP 5 Score: 135.576 bits (340), Expect = 1.343e-30 Identity = 132/637 (20.72%), Postives = 253/637 (39.72%), Query Frame = -2
Query: 2160 SSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEKP-AFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPE----------------------------------RTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVD-KTMYTSLINVYCKGRKMKMALRVYYRMLK-MGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQME-DRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTAL 3956
S + + S + +A+ + + G + D+ LI L ++ + A+ + + + P N+++ L K GK++EA LF+ ++ FN L LC + A KM+++ + YN +I GL + G ++EA+ F M + P ++ L + + + A S ++ + G D ++ +I CK + A ++ + K +G +P TYN LI G ++ + + +F Q+ G P V T++ ++ Y + GK+D L M + N + ++I+ L K + +A + Y Y+L M + + C + PL+ +S + L + L E +L +N+LI+ + + D+A +M G P L TY+ L+ CL G ++ ++ G+ PD +IIN K LE A + ++M+ RG+ P + YN +I +L V EA ++ + +G++P+ + +I G+S +G+ A ++ M+ P + +Y L
Sbjct: 503 SVTYNMMMKCYSKVGEIDEAIKLLSEMMENGCEPDVIVVNSLINTLYKADRVDEAWKMFM-RMKEMKLKPTVVTYNTLLAGLGKNGKIQEAIELFEGMVQKGCPPNTITFNTLFDCLCKNDEVTLALKMLFKMMDMGCVPDVFTYNTIIFGLVKNGQVKEAMCFFHQMKKLVYPDFVTLCTLLPGVVKASLIEDAYKIITNFLYNCADQPANLFWEDLIGSILAEAGIDNAVSFSERLVANGICRDGDSILVPIIRYSCKHNNVSGARTLFEKFTKDLGVQPKLPTYNLLIGGLLEADMIEIAQDVFLQVKSTGCIPDVATYNFLLDAYGKSGKIDELFELYKEMSTHECEANTITHNIVISGLVKAGNVDDALDLY-------------------------YDL----MSDRDFSPTACTYGPLIDGLSKSGRLYEAKQ-LFEGMLDYGCRPNCAIYNILINGFGKAGEADAACALFKRMVKEGVRPDLKTYSVLVDCLCMVGRVDEGLHYFKELKESGLNPDVVCYNLIINGLGKSHRLEEALVLFNEMKTSRGITPDLYTYNSLILNLGIAGMVEEAGKIYNEIQRAGLEPNVFTFNALIRGYSLSGKPEHAYAVYQTMVTGGFSPNTGTYEQL 1108 HSP 6 Score: 116.701 bits (291), Expect = 8.657e-25 Identity = 108/450 (24.00%), Postives = 180/450 (40.00%), Query Frame = -2
Query: 1167 TCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRV---------IEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTFRK 2489
TC ++ G LE + D M+ R +K Y I SL + + +A +M E G ++N +S NG + EA ++ MI RP+ +Y++L+ GL K+ D L++M GL PN +T I + + A+ ++ MD D++ Y L+ ++RK + C A ++ +K+K R P+ Y ++ F + + + M+K+G +P+ VTFTIL+ KAG A + M G P+ Y L+ GL + RL +AL L M G+ P+ TY + + Y+ S +V A + FE+M P + CN L L + REA ++ + G P T ++ +K + K
Sbjct: 120 TCNYMLEALRVDGKLEEMAYVFDLMQKRIIKRDTNTYLTIFKSLSVKGGLKQAPYALRKMREFGF---------VLNAYSYNGLIHLLLKSRFCTEAMEVYRRMILEGFRPSLQTYSSLMVGLGKRRDIDSVMGLLKEMETLGLKPNVYTFTICIRVLGRAGKINEAYEILKRMDDEGCGPDVVTYTVLIDAL---CTARK-----------------------------------------LDC-AKEVFEKMKTGRHKPDRVTYITLLDRFSDNRDLDSVKQFWSEMEKDGHVPDVVTFTILVDALCKAGNFGEAFDTLDVMRDQGILPNLHTYNTLICGLLRVHRLDDALELFGNMESLGVKPTAYTYIVFIDYYGKSGDSVSALETFEKMKTKGIAPNIVACNASLYSLAKAGRDREAKQIFYGLKDIGLVPDSVTYNMMMKCYSK 515
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy Swiss-Prot
Match: PP360_ARATH (Pentatricopeptide repeat-containing protein At5g01110 OS=Arabidopsis thaliana GN=At5g01110 PE=2 SV=1) HSP 1 Score: 231.876 bits (590), Expect = 6.029e-62 Identity = 143/552 (25.91%), Postives = 260/552 (47.10%), Query Frame = -2
Query: 1878 YNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQ--IERDLIFYISLVSGFYRSISSRKRWFAMRRGSERA 3527
+++LI Q L EA + F L+ + ++ ++ +L L + GWV A ++ ++ G ++ ++N CK KM+ ++ + G PD TYNTLI + GL + + L N M +G P V T++ +I+ C+ GK + A + M+ L+P++ Y L+ K+ + E E+ + M + +VPD V F +M ++ + LD A M ++ G L+P V + +LI C + A ++M GC + TYN+++ L ++ + +A+ L N M + +FPD T I+I+ HCK GNL+ A ++ +M+++ ++ V YN ++ + + A ++ M+ + P I Y ++N + G + EA R++ EMI I+PT ++I G + G ++LEKM+ +G VP+ + Y +LI F+++ AF LV M+ Q + D+ Y S++ GF R ++ +R+ ER
Sbjct: 168 FDLLIRTYVQARKLREAHEAFTLLRSKGFTV-SIDACNALIGSLVRIGWVELAWGVYQEISRSGVGINVYTLNIMVNALCKDGKMEKVGTFLSQVQEKGVYPDIVTYNTLISAYSSKGLMEEAFELMNAMPGKGFSPGVYTYNTVINGLCKHGKYERAKEVFAEMLRSGLSPDSTTYRSLLMEACKKGDVVETEKVFSDMRSRDVVPDLVCFSSMMSLFTRSGNLDKALMYFNSVKEAG------LIP-------------------------DNVIYTILIQGYCRKGMISVAMNLRNEMLQQGCAMDVVTYNTILHGLCKRKMLGEADKLFNEMTERALFPDSYTLTILIDGHCKLGNLQNAMELFQKMKEKRIRLDVVTYNTLLDGFGKVGDIDTAKEIWADMVSKEILPTPISYSILVNALCSKGHLAEAFRVWDEMISKNIKPTVMICNSMIKGYCRSGNASDGESFLEKMISEGFVPDCISYNTLIYGFVREENMSKAFGLVKKMEEEQGGLVPDVFTYNSILHGFCRQNQMKEAEVVLRKMIERG 687 HSP 2 Score: 201.06 bits (510), Expect = 8.326e-52 Identity = 147/578 (25.43%), Postives = 263/578 (45.50%), Query Frame = -2
Query: 2283 SFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNS-CEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAF-----MVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQ--GIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDE 3992
S N G S L R + + +A + S+G + + LI LV+ G + LA+ Y + I G+ + N +V LCK GK+E+ ++ + +N L+ S+ +AF+ M + G + YN +I+GLC+ G E A ++F M R+ P Y+SL CK+G VVE E +F M S+ D ++S+++++ + + AL + + + G PD+ Y LI G+ + G+ L N+M++QG VVT++ ++ C+ + A L N M + L P+++ T+LI K + A E +++M + D V + L+ + K ++D A MV K I + L+ + + L + + +E++ N+ + N +I C F++KM S G +P +YN+LI ++ A L+ +M+ + G+ PD T I++ C+ ++ A +L +M +RG+ P + Y C+I Q ++EA + ML+ G PD+
Sbjct: 153 SLDSTFSNCGSNDSVFDLLIRTYVQARKLREAHEAFTLLRSKGFTVSIDACNALIGSLVRIGWVELAWGVYQE-ISRSGVGINVYTLNIMVNALCKDGKMEKVGTFLSQVQEKGVYPDIVTYNTLISAYSSKGLMEEAFELMNAMPGKGFSPGVYTYNTVINGLCKHGKYERAKEVFAEML-RSGLSPDSTTYRSLLMEACKKGDVVETEKVFSDMRSRDVVPDLVCFSSMMSLFTRSGNLDKALMYFNSVKEAGLIPDNVIYTILIQGYCRKGMISVAMNLRNEMLQQGCAMDVVTYNTILHGLCKRKMLGEADKLFNEMTERALFPDSYTLTILIDGHCKLGNLQNAMELFQKMKEKRIRLDVVTYNTLLDGFGKVGDIDTAKEIWADMVSKEILPTPISYSILVNALCSKGHLAEAFRVW-DEMISKNIKPTVMICNSMIKGYCRSGNASDGESFLEKMISEGFVPDCISYNTLIYGFVREENMSKAFGLVKKMEEEQGGLVPDVFTYNSILHGFCRQNQMKEAEVVLRKMIERGVNPDRSTYTCMINGFVSQDNLTEAFRIHDEMLQRGFSPDD 727 HSP 3 Score: 188.348 bits (477), Expect = 8.870e-48 Identity = 122/513 (23.78%), Postives = 237/513 (46.20%), Query Frame = -2
Query: 1173 AFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCF--ALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPG--PQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTF 2699
A N LI +L + A ++ G ++T N ++ L + G E + L+++Q +G++PD T +I+ + G +E AF++++ M +G PGV YN +I L + K A +F ML SG+ PD Y +++ G V+E +++F +M + P +++++S + DK Y + + GL+P+ V+YT LI + +K A L + M + D++ Y +++ G +R +KLF + ++L P L + + A++L QK+K+ R ++ YN ++ GF + + A + + M + +LP ++++IL+ G + A ++++M + P ++ ++ G C++ + S L M G +P +Y ++ F AF + ++M G P ++ N +L C Q +++EA +V +I+RG P + T + I F
Sbjct: 202 ACNALIGSLVRIGWVELAWGVYQEISRSGVGINVYTLNIMVNALCKDGKMEKVGTFLSQVQEKGVYPDIVTYNTLISAYSSKGLMEEAFELMNAMPGKGFSPGVYTYNTVINGLCKHGKYERAKEVFAEMLRSGLSPDSTTYRSLLMEACKKGDVVETEKVFSDMRSRDVVPDLVCFSSMMSLFTRSGNLDKALMYFNSVKEAGLIPDNVIYTILIQGYCRKGMISVAMNLRNEMLQQGCAMDVVTYNTILHGL------------CKRKMLGEADKLFNEMTERALFPDSYTLTILIDGHCKLGNLQNAMELFQKMKEKRIRLDVVTYNTLLDGFGKVGDIDTAKEIWADMVSKEILPTPISYSILVNALCSKGHLAEAFRVWDEMISKNIKPTVMICNSMIKGYCRSGNASDGESFLEKMISEGFVPDCISYNTLIYGFVREENMSKAFGLVKKMEEEQGGLVPDVFTYNSILHGFCRQNQMKEAEVVLRKMIERGVNPDRSTYTCMINGF 702 HSP 4 Score: 182.57 bits (462), Expect = 5.375e-46 Identity = 132/581 (22.72%), Postives = 261/581 (44.92%), Query Frame = -2
Query: 1314 NLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPD----HVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSAN----EELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSL-LPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNAD--GCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGP 3023
N N + +LI + + ++ EA E + + + G + L L+++ G+ +++A+ V + I+ +G G + L + N + +K+ + ++ + ++ V +N LISA + + A M+ M G P ++TYN++I L + G +E A+ + M G+ PD T ++ E CK G++ + M R + P + ++ ++ R + +A F + E+G+ PD +IY +I G+ G + A L +EM+ +Y ++ GL K+ M + +M + L P++ T LI + + A L M +I D++ Y +L+ GF + + A+E ++ ++ L P ++ V+ + A ++ ++ P + + N +I G+C + D E M EG +P+ +++ L+ G ++ + A GL KM + G PD Y +++G C+ +++ EA +L M +RG+ P + TY ++ F AF+I +EM+ P
Sbjct: 160 NCGSNDSVFDLLIRTYVQARKLREAHEAFTLLRSKGFTVSIDACNALIGSLVRI---GW-VELAWGVYQEISRSGVGINVYTLNIMVNALCKDGKMEKVGTFLSQVQEKGVYPDIVTYNTLISAYSSKGLMEEAFELMNAMPGKGFSPGVYTYNTVINGLCKHGKYERAKEVFAEMLRSGLSPDSTTYRSLLMEACKKGDVVETEKVFSDMRSRDVVPDLVCFSSMMSLFTRSGNLDKALMYFNSVKEAGLIPDNVIYTILIQGYCRKGMISVAMNLRNEMLQQGCAMDVVTYNTILHGLCKRKMLGEADKLFNEMTERALFPDSYTLTILIDGHCKLGNLQNAMELFQKMKEKRIRLDVVTYNTLLDGFGKV-----------GDIDTAKEIWADMVSKEILPTPISYSILVNALCSKGHLAEAFRVWDEMISKNIKPTVMICNSMIKGYCRSGNASDGESFLEKMISEGFVPDCISYNTLIYGFVREENMSKAFGLVKKMEEEQGGLVPDVFTYNSILHGFCRQNQMKEAEVVLRKMIERGVNPDRSTYTCMINGFVSQDNLTEAFRIHDEMLQRGFSP 725 HSP 5 Score: 179.489 bits (454), Expect = 4.462e-45 Identity = 166/710 (23.38%), Postives = 316/710 (44.51%), Query Frame = -2
Query: 1995 FKSRRAVTTSAALPLDPSYATISSIPADHFSLCLSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAV--LIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLY--KSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIV----ILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEM--IGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLM 4094
FK + +TTS++ +PS ++ SS + FS+ SF + I L K V N + L+ V+ + DL L V L H L+ S ++ G + D+ +I + +LE L D ++ F+ L+R + +A + F + + + N LI L + G++E A ++ E + S +++Y + LCK G + + + +++ +G + D Y +LI+ Y M+ A + M G P YTYNT+I+G K G ++ +F +M+ GL P T+ ++ C++G V + ++M S+++ P+ C++ +++ + + +A ++ + GL+PD+V++ L++ Y + + +A + + GC D + + ++K++ L E+ + L +LI C+ +A KM+ + TYN+L+ + G + A+ + M ++ I P + I++N C G+L AF + D+M + +KP V I N +I R S+ + +M+ G PD I Y T+I GF + +A L +M + P +Y +++ G ++N + L KM++ G+ P+ YT +I+ F+ + AFR+ D M
Sbjct: 18 FKPLKNLTTSSSPVFEPSSSSSSSSSSASFSVSDSFLVEKICFSL-----KQGNNNVRNHLIRLNPLAVVEVLYRCRNDLTLGQRFVDQLGFHFPNFKHTSLSLSAMIHILVRSGRLSDAQSCLLRMIRRSGVSRLEIVNSL-DSTFSNCGSNDSVFDLLIRTYVQARKLREAHEAFTLLRSKGFTVSIDACNALIGSLVRIGWVELAWGVYQ---EISRSGVGINVYTLNIMVNALCKDGKMEKVGTFLSQVQEKGVYPDIVTYNTLISAYSSKGLMEEAFELMNAMPGKGFSPGVYTYNTVINGLCKHGKYERAKEVFAEMLRSGLSPDSTTYRSLLMEACKKGDVVETEKVFSDMRSRDVVPDLVCFSSMMSLFTRSGNLDKALMYFNSVKEAGLIPDNVIYTILIQGYCRKGMISVAMNLRNEMLQQGCAMDVVTYNTILHGLCKRKMLGEADKLFNEMTERALFPDSYTLTILIDGHCKLGNLQNAMELFQKMKEKRIRLDVVTYNTLLDGFGKVGDIDTAKEIWADMVSKEILPTPISYSILVNALCSKGHLAEAFRVWDEMISKNIKPTVMICNSMIKGYCRSGNASDGESFLEKMISEGFVPDCISYNTLIYGFVREENMSKAFGLVKKMEEEQGGLVPDVFTYNSILHGFCRQNQMKEAEVVLRKMIERGVNPDRSTYTCMINGFVSQDNLTEAFRIHDEM 718 HSP 6 Score: 172.17 bits (435), Expect = 1.081e-42 Identity = 131/606 (21.62%), Postives = 253/606 (41.75%), Query Frame = -2
Query: 1521 DKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPK--EKNLRVSDSSPEAMKCFALKLIQKVK--QTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPD 3326
+ +++ LI Y + RK++ A + + G N LI V++G + W ++ ++ G+ +V T ++M++ C++GK++ L+ + K + P+ Y LI++ + + EA E M G P + ++ K + + A V E+L+S L + L+ C++ M S +P L ++S++ ++ G + A N ++ G+ PD I+I +C+ G + A ++ ++M +G V YN I+ L ++K + EA +F M E + PD +I+G G + A LF +M IR +Y L+ G K D M+ ++P + Y+ L++ K AFR+ D M I+ ++ S++ G+ RS G+ E + + + +P N + E A L++K++ Q +P+++ YN I+ GFC ++M++A M + GV P++ T+T ++ G + + A + ++M G +PD
Sbjct: 164 NDSVFDLLIRTYVQARKLREAHEAFTLLRSKGFTVSIDACNALIGSLVRIGWVELAWGVYQEISRSGVGINVYTLNIMVNALCKDGKMEKVGTFLSQVQEKGVYPDIVTYNTLISAYSSKGLMEEAFELMNAMPGKGFSPGVYTYNTVINGLCKHGKYERAKEVFA-------------------------------EMLRSGLSPDSTTYRSLLMEACKKGDVVETEKVFSDMRSRDVVPDLVCFSSMMSLFTRSGNLDKALMYFNSVKEAGLIPDNVIYTILIQGYCRKGMISVAMNLRNEMLQQGCAMDVVTYNTILHGLCKRKMLGEADKLFNEMTERALFPDSYTLTILIDGHCKLGNLQNAMELFQKMKEKRIRLDVVTYNTLLDGFGKVGDIDTAKEIWADMVSKEILPTPISYSILVNALCSKGHLAEAFRVWDEMISKNIKPTVMICNSMIKGYCRS------------GNASDGESFLEKMISEGFVPDCISYNTLIYGFVREENMSKAFGLVKKMEEEQGGLVPDVFTYNSILHGFCRQNQMKEAEVVLRKMIERGVNPDRSTYTCMINGFVSQDNLTEAFRIHDEMLQRGFSPD 726 HSP 7 Score: 145.976 bits (367), Expect = 3.079e-34 Identity = 104/463 (22.46%), Postives = 201/463 (43.41%), Query Frame = -2
Query: 1209 YNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEK--NLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYP 2591
++ LI+ Q +A ++++G C +I + G +E A+ + ++ G+ V N ++ +L + K+ + ++ E GV PD + Y T+I+ +S+ G + EA L + M G P ++Y +I+GL K ++ +ML GL P++ Y SL+ +K + ++ M + DL+ + S++S F RS G+ F + L+P + + + M A+ L ++ Q ++ YN I+ G C+ + +A F M + + P+ T TIL+ GH K G + +A+ LF KM D + Y L++G + + A + M + ++P+ +Y I++ C AF++++EM+ + P + CN ++ C + + +I G P
Sbjct: 168 FDLLIRTYVQARKLREAHEAFTLLRSKGFTVSIDACNALIGSLVRIGWVELAWGVYQEISRSGVGINVYTLNIMVNALCKDGKMEKVGTFLSQVQEKGVYPDIVTYNTLISAYSSKGLMEEAFELMNAMPGKGFSPGVYTYNTVINGLCKHGKYERAKEVFAEMLRSGLSPDSTTYRSLLMEACKKGDVVETEKVFSDMRSRDVVPDLVCFSSMMSLFTRS------------GNLDKALMYFNSVKEAGLIPDNVIYTILIQGYCRKGMISVAMNLRNEMLQQGCAMDVVTYNTILHGLCKRKMLGEADKLFNEMTERALFPDSYTLTILIDGHCKLGNLQNAMELFQKMKEKRIRLDVVTYNTLLDGFGKVGDIDTAKEIWADMVSKEILPTPISYSILVNALCSKGHLAEAFRVWDEMISKNIKPTVMICNSMIKGYCRSGNASDGESFLEKMISEGFVP 618 HSP 8 Score: 68.9366 bits (167), Expect = 2.869e-10 Identity = 41/190 (21.58%), Postives = 95/190 (50.00%), Query Frame = -2
Query: 1167 NLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPT-KSLFIKTFRK 1733
N +++++I + +A ++++A++ F L++ +G + L+G ++ G ++ A G++ +++ G + I+VN LC+ ++ + + L + ++G+ P TY ++ + L AF++ M P +Y N ++ LC K A V+ +++ G P T +SL ++ +K
Sbjct: 164 NDSVFDLLIRTYVQARKLREAHEAFTLLRSKGFTVSIDACNALIGSLVRIGWVELAWGVYQEISRSGVGINVYTLNIMVNALCKDGKMEKVGTFLSQVQEKGVYPDIVTYNTLISAYSSKGLMEEAFELMNAMPGKGFSPGVYTYNTVINGLCKHGKYERAKEVFAEMLRSGLSPDSTTYRSLLMEACKK 353
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy Swiss-Prot
Match: PP120_ARATH (Putative pentatricopeptide repeat-containing protein At1g74580 OS=Arabidopsis thaliana GN=At1g74580 PE=3 SV=1) HSP 1 Score: 230.72 bits (587), Expect = 2.487e-61 Identity = 181/726 (24.93%), Postives = 322/726 (44.35%), Query Frame = -2
Query: 1332 CQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKM-ESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKE-GVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMV 3503
CQK ++ A++MF+ M + TL Y+S+ L G E + M E+ G + + +Y + Y + K++ A+ V+ RM C P ++YN ++ V G FD ++ +M ++G+ P V +F + + ++C+ + A LLNNM S+ N Y ++ Y+EN E E + +ML G+ F KL+++ K ++ +E E+ L+++++K + +N+ I LC++ + D A + + G P + TYN+LI L + F++AE L +M +G+ PD T +I +CK G ++ A I+ G P Y +I L + + + A A+F L G+ P+ I+Y T+I G SN G ++EA +L +EM + P ++ L++GL K ++ M+ G P+ + LI + + + E A ++D+M N ++ D+ Y SL++G ++ + E+ L LL R D AL L++++K P+ + +I GFC+ + AY F M++ V + T+ I++ + + A LF +M PDG Y+++V+G C+ + L M + G IPS T ++ C A I MV
Sbjct: 16 CQKDPMK-ALEMFNSMRKEVGFKHTLSTYRSVIEKLGYYGKFEAMEEVLVDMRENVGNHMLEGVYVGAMKNYGRKGKVQEAVNVFERMDFYDCEPTVFSYNAIMSVLVDSGYFDQAHKVYMRMRDRGITPDVYSFTIRMKSFCKTSRPHAALRLLNNMSSQGCEMNVVAYCTVVGGFYEENFKAEGYELFGKMLASGVSLCLSTFNKLLRVLCKKGDV---------------------------KECEK----LLDKVIKRGVLPNLFTYNLFIQGLCQRGELDGAVRMVGCLIEQGPKPDVITYNNLIYGLCKNSKFQEAEVYLGKMVNEGLEPDSYTYNTLIAGYCKGGMVQLAERIVGDAVFNGFVPDQFTYRSLIDGLCHEGETNRALALFNEALGKGIKPNVILYNTLIKGLSNQGMILEAAQLANEMSEKGLIPEVQTFNILVNGLCKMGCVSDADGLVKVMISKGYFPDIFTFNILIHGYSTQLKMENALEILDVMLDNGVDPDVYTYNSLLNGLCKTSKFEDVMETYKTMVEKGCAP--NLFTFNILLESLCRYRKLDE--------ALGLLEEMKNKSVNPDAVTFGTLIDGFCKNGDLDGAYTLFRKMEEAYKVSSSTPTYNIIIHAFTEKLNVTMAEKLFQEMVDRCLGPDGYTYRLMVDGFCKTGNVNLGYKFLLEMMENGFIPSLTTLGRVINCLCVEDRVYEAAGIIHRMV 699 HSP 2 Score: 219.55 bits (558), Expect = 9.442e-58 Identity = 165/667 (24.74%), Postives = 293/667 (43.93%), Query Frame = -2
Query: 2070 PLDPSYATISSIPAD--HFSLCLSFSEQLINRGLLSSARKLFQRVVS--NSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDR-GMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAV 4055
P SY I S+ D +F ++ +RG+ R+ S +S AL ++ ++S+G ++++ Y ++ + Y + +A+ + +CL FN L+R+LC + + K+I V + YN+ I GLCQ+G L+ A++M + E+ P + Y +L YGLCK EAE GKM ++G D Y +LI YCKG +++A R+ + G PD +TY +LI G G + LFN+ + +G++P+V+ ++ +I +G + A+ L N M K L P + +L+ L K +++A+ + M++ G PD F L+ Y +++ A +L + N G DP + +N L++ LC+ ++ + M GC P LFT+N L++ L + ++A LL M+ + + PD T +I+ CK+G+L+ A+ + +ME+ + YN II + + V+ A +F M++ + PD Y M++GF G V + EM+ P+ + +I+ L ++ + + +M+ GLVP AV
Sbjct: 109 PTVFSYNAIMSVLVDSGYFDQAHKVYMRMRDRGITPDVYSFTIRMKSFCKTSRPHAALRLLNNMSSQGCEMNVVAYCTVVGGFYEENFKAEGYELFG-----------KMLASGVSLCL------------------------STFNKLLRVLCKKGDVKECEKLLDKVIKRGVLPNLFTYNLFIQGLCQRGELDGAVRMVGCLIEQGPK-PDVITYNNLIYGLCKNSKFQEAEVYLGKMVNEGLEPDSYTYNTLIAGYCKGGMVQLAERIVGDAVFNGFVPDQFTYRSLIDGLCHEGETNRALALFNEALGKGIKPNVILYNTLIKGLSNQGMILEAAQLANEMSEKGLIPEVQTFNILVNGLCKMGCVSDADGLVKVMISKGYFPDIFTFNILIHGYSTQLKMENALEILDVMLDN--GVDPDVY-----------------------------TYNSLLNGLCKTSKFEDVMETYKTMVEKGCAPNLFTFNILLESLCRYRKLDEALGLLEEMKNKSVNPDAVTFGTLIDGFCKNGDLDGAYTLFRKMEEAYKVSSSTPTYNIIIHAFTEKLNVTMAEKLFQEMVDRCLGPDGYTYRLMVDGFCKTGNVNLGYKFLLEMMENGFIPSLTTLGRVINCLCVEDRVYEAAGIIHRMVQKGLVPEAV 708 HSP 3 Score: 200.675 bits (509), Expect = 1.117e-51 Identity = 161/669 (24.07%), Postives = 288/669 (43.05%), Query Frame = -2
Query: 1305 VYCKGRKMKMALRVYYRMLK-MGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHV-MISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPK--EKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKG-TYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLY 3296
+ C+ MK AL ++ M K +G + TY ++I G F+ + M E + +V + NY R+GKV A + M + P Y +++ L +A + Y RM + G+ PD F MK + K A +L ++ GC + VA+ ++ E+ + KM + G L T+N L++ L +KG ++ E LL+++ +G+ P+ T + I C+ G L+ A ++ + ++G KP V YN +I L + K EA +M+ G++PD Y T+I G+ G V A+R+ + + P +Y +LI GL + T++ + L G+ PN +LY +LI + A +L + M + ++ + LV+G + G + L +++ + P N+ + S + AL+++ + P++Y YN +++G C+ + +D + ++ M ++G PN TF IL+ + ++D A+GL +M PD + + L++G C+ L A +L M + + S TY II+ F + A K+F+EMV GP Y
Sbjct: 14 IKCQKDPMK-ALEMFNSMRKEVGFKHTLSTYRSVIEKLGYYGKFEAMEEVLVDMRENVGNHMLEGVYVGAMKNYGRKGKVQEAVNVFERMDFYDCEPTVFSYNAIMSVLVDSGYFDQAHKVYMRMRDRGITPDVYSFTIRMKSFCKTSRPHAALRLLNNMSSQGC-------------------------------EMNVVAYCTVVGGFYEENFKAEGYELFGKMLASGVSLCLSTFNKLLRVLCKKGDVKECEKLLDKVIKRGVLPNLFTYNLFIQGLCQRGELDGAVRMVGCLIEQGPKPDVITYNNLIYGLCKNSKFQEAEVYLGKMVNEGLEPDSYTYNTLIAGYCKGGMVQLAERIVGDAVFNGFVPDQFTYRSLIDGLCHEGETNRALALFNEALGKGIKPNVILYNTLIKGLSNQGMILEAAQLANEMSEKGLIPEVQTFNILVNGL------------CKMGCVSDADGLVKVMISKGYFPDIFTFNILIHGYSTQLKMENALEILDVMLDNGVDPDVYTYNSLLNGLCKTSKFEDVMETYKTMVEKGCAPNLFTFNILLESLCRYRKLDEALGLLEEMKNKSVNPDAVTFGTLIDGFCKNGDLDGAYTLFRKMEEAYKVSSSTPTYNIIIHAFTEKLNVTMAEKLFQEMVDRCLGPDGY 638 HSP 4 Score: 193.356 bits (490), Expect = 2.537e-49 Identity = 152/666 (22.82%), Postives = 281/666 (42.19%), Query Frame = -2
Query: 1617 QKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMM-----------------------------------EQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRN-QIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLR--VSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVT 3500
+KG ++EA+ +F+ M + + PT+ Y ++ L G+ +A ++ +M +G D +T + +CK + ALR+ M GC + Y T++ GF + G+ LF +M+ ++G+ P++ T+++ I C+ G++D A ++ +I + P+ Y LI L K ++ EAE + +M+N GL PD + L+ Y KG + +A ++ NG F P + + LI LC + + + A ++ G P + YN+LIK LS +G+ +A L N M +G+ P+ T I++N CK G + A ++ M +G P + +N +I Q K+ A + ML++GVDPD Y +++NG + + + M+ P ++ L+ L + D+ LE+M + + P+AV + +LI F + + + A+ L M+ ++ Y ++ F ++ EKLFQ + + L P R V K + ++ + F+P+L +I+ C DR+ +A M ++G++P V
Sbjct: 88 RKGKVQEAVNVFERM-DFYDCEPTVFSYNAIMSVLVDSGYFDQAHKVYMRMRDRGITPDVYSFTIRMKSFCKTSRPHAALRLLNNMSSQGCEMNVVAYCTVVGGFYEENFKAEGYELFGKMLASGVSLCLSTFNKLLRVLCKKGDVKECEKLLDKVIKRGVLPNLFTYNLFIQGLCQRGELDGAVRMVGCLIEQGPKPDVITYNNLIYGLCKNSKFQEAEVYLGKMVNEGLEPDSYTYNTLIAGYCKGGMVQLAERIVGDAVFNG--FVP-----------------------------DQFTYRSLIDGLCHEGETNRALALFNEALGKGIKPNVILYNTLIKGLSNQGMILEAAQLANEMSEKGLIPEVQTFNILVNGLCKMGCVSDADGLVKVMISKGYFPDIFTFNILIHGYSTQLKMENALEILDVMLDNGVDPDVYTYNSLLNGLCKTSKFEDVMETYKTMVEKGCAPNLFTFNILLESLCRYRKLDEALGLLEEMKNKSVNPDAVTFGTLIDGFCKNGDLDGAYTLFRKMEEAYKVSSSTPTYNIIIHAFTEKLNV------------TMAEKLFQEMVDRCLGPDGYTYRLMVDGFCKTGNVNLGYKFLLEMMENGFIPSLTTLGRVINCLCVEDRVYEAAGIIHRMVQKGLVPEAVN 709 HSP 5 Score: 164.851 bits (416), Expect = 2.834e-40 Identity = 124/499 (24.85%), Postives = 216/499 (43.29%), Query Frame = -2
Query: 1167 QDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLR--VSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTFRK 2657
Q++ + F ++M+ C P +F+YN+++ L G F+ A + RM+ +GI PD + I + CK A +L+ M +G + V Y ++G + + +E +F +ML SGV + ++ G V E ++L ++I + P +Y I GL ++ D + +++ G P+ + Y +LI + +F+ A + M +E D Y +L++G+ + G + E++ +P + R + E AL L + PN+ LYN +I G + +A M ++G++P TF IL+ G K G + A GL M + G PD + IL++G ++ AL +L VM G+ P TY +L C + + ++ MV P L+ N LL LC +KL EA + + + + P T I F K
Sbjct: 93 QEAVNVF-ERMDFYDCEPTVFSYNAIMSVLVDSGYFDQAHKVYMRMRDRGITPDVYSFTIRMKSFCKTSRPHAALRLLNNMSSQGCEMNVVAYCTVVGGFYEENFKAEGYELFGKMLASGVSLCLSTFNKLLRVLCKKGDVKECEKLLDKVIKRGVLPNLFTYNLFIQGLCQRGELDGAVRMVGCLIEQGPKPDVITYNNLIYGLCKNSKFQEAEVYLGKMVNEGLEPDSYTYNTLIAGYCKG------------GMVQLAERIVGDAVFNGFVPDQFTYRSLIDGLCHEGETNRALALFNEALGKGIKPNVILYNTLIKGLSNQGMILEAAQLANEMSEKGLIPEVQTFNILVNGLCKMGCVSDADGLVKVMISKGYFPDIFTFNILIHGYSTQLKMENALEILDVMLDNGVDPDVYTYNSLLNGLCKTSKFEDVMETYKTMVEKGCAPNLFTFNILLESLCRYRKLDEALGLLEEMKNKSVNPDAVTFGTLIDGFCK 578
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy Swiss-Prot
Match: RF1_ORYSI (Protein Rf1, mitochondrial OS=Oryza sativa subsp. indica GN=Rf1 PE=2 SV=1) HSP 1 Score: 230.335 bits (586), Expect = 4.034e-61 Identity = 176/703 (25.04%), Postives = 302/703 (42.96%), Query Frame = -2
Query: 1182 ALRVYYRMLKMGC---RPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKV-DCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLN---GGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLV-DLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFI 3266
A+ Y RM + G PD TY LI + G D G+ ++++G + + F ++ C + + D ++L M PN Y +L+ L ENR EA E M + GG PD V + ++ + K + D A+ E+L + V +N +I+ALC+ D A ++ M G MP TYNS++ G ++A L +M++ G+ PD T ++++ CK+G A I D M RG+KP + Y ++ + + E + M+ +G+ PD ++ +I ++ G+V +A +F +M + P + +Y A+I L K + Y E+M+D+GL P ++Y SLI ++E A L+ +++DR I + IF+ S++ + G EKLF+L+ R + PN+ YN +I+G+C A +M +A M G+ PN VT++ L+ G+ K ++ A+ LF +M + G +PD I Y I++ GL Q R A L + + G TY IIL C + L A ++F+ LC++ ++ + R I+ D++++ G+ K LF+
Sbjct: 68 AVSRYNRMARAGADEVTPDLCTYGILIGCCCRAGRLDLGFAALGNVIKKGFRVDAIAFTPLLKGLCADKRTSDAMDIVLRRMTELGCIPNVFSYNILLKGLCDENRSQEALELLHMMADDRGGGSPPDVVSYTTVINGFFKEGDSDKAYSTY-------------------------------HEMLDRGILPDVVTYNSIIAALCKAQAMDKAMEVLNTMVKNGVMPDCMTYNSILHGYCSSGQPKEAIGFLKKMRSDGVEPDVVTYSLLMDYLCKNGRCMEARKIFDSMTKRGLKPEITTYGTLLQGYATKGALVEMHGLLDLMVRNGIHPDHYVFSILICAYAKQGKVDQAMLVFSKMRQQGLNPNAVTYGAVIGILCKSGRVEDAMLYFEQMIDEGLSPGNIVYNSLIHGLCTCNKWERAEELILEMLDRG-ICLNTIFFNSIIDSH------------CKEGRVIESEKLFELMVRIGV---------------------------------KPNVITYNTLINGYCLAGKMDEAMKLLSGMVSVGLKPNTVTYSTLINGYCKISRMEDALVLFKEMESSGVSPDIITYNIILQGLFQTRRTAAAKELYVRITESGTQIELSTYNIILHGLCKNKLTDDALQMFQN----------------LCLMDLKLEARTFNIMIDALLKVGR--NDEAKDLFV 675 HSP 2 Score: 220.705 bits (561), Expect = 5.953e-58 Identity = 152/626 (24.28%), Postives = 278/626 (44.41%), Query Frame = -2
Query: 2022 IIPDSSIANSIVICLCKLGKLEEATILFDRLLTDN-SCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGC----WYYNVLIDGLCQKGYLEEAIQMFDLMPERTE--SLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSA----------------------NEELEQKIVI-------------------LIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFE 3755
+ PD ++ C C+ G+L+ ++ + AF L++ LC+ +R DA D ++ + LGC + YN+L+ GLC + +EA+++ +M + S P + Y ++ G K+G +A S + +M +G D Y S+I CK + M A+ V M+K G PD TYN+++HG+ G +M G++P VVT+ +++ C+ G+ A + ++M + L P Y L+ + + E + M+ G+ PDH +F L+ Y K ++D A +V + G +P + A +E L ++ LI E+L + L + FN +I + C++ + + + M +G P + TYN+LI G ++A LL+ M + G+ P+ T +IN +CK +E A + +ME G+ P + YN I+ LF+ ++ + A ++VR+ ESG + Y +++G N +A ++F + ++ + ++ +I L+K D+ +GLVPN Y + N + + E
Sbjct: 83 VTPDLCTYGILIGCCCRAGRLDLGFAALGNVIKKGFRVDAIAFTPLLKGLCADKRTSDAMDIVLRRM---TELGCIPNVFSYNILLKGLCDENRSQEALELLHMMADDRGGGSPPDVVSYTTVINGFFKEGDSDKAYSTYHEMLDRGILPDVVTYNSIIAALCKAQAMDKAMEVLNTMVKNGVMPDCMTYNSILHGYCSSGQPKEAIGFLKKMRSDGVEPDVVTYSLLMDYLCKNGRCMEARKIFDSMTKRGLKPEITTYGTLLQGYATKGALVEMHGLLDLMVRNGIHPDHYVFSILICAYAKQGKVDQAMLVFSKMRQQG--LNPNAVTYGAVIGILCKSGRVEDAMLYFEQMIDEGLSPGNIVYNSLIHGLCTCNKWERAEELILEMLDRGICLNTIFFNSIIDSHCKEGRVIESEKLFELMVRIGVKPNVITYNTLINGYCLAGKMDEAMKLLSGMVSVGLKPNTVTYSTLINGYCKISRMEDALVLFKEMESSGVSPDIITYNIILQGLFQTRRTAAAKELYVRITESGTQIELSTYNIILHGLCKNKLTDDALQMFQNLCLMDLKLEARTFNIMIDALLKVGRNDEAKDLFVAFSSNGLVPNYWTYRLMAENIIGQGLLE 703 HSP 3 Score: 206.068 bits (523), Expect = 3.095e-53 Identity = 159/680 (23.38%), Postives = 296/680 (43.53%), Query Frame = -2
Query: 1431 ESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALR-VYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQ---GLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFD----PLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLP-KEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKR 3443
E P L Y L C+ G + + G + +GF VD +T L+ C ++ A+ V RM ++GC P+ ++YN L+ G L + M + G P VV++ +I+ + +EG D A + M+ + + P+ Y +I +L K + +A E M+ G++PD + + ++ Y + A LK + +G D LL+ + + + + K L + L+ + +D M G P + ++ LI +++G + A + ++M+ QG+ P+ T +I CK G +E A +QM D G+ PG +YN +I L K A + + ML+ G+ + I + ++I+ GRVIE+++LF M+ ++P +Y LI+G D+ L M+ GL PN V Y++LI+ + + E A L M+ + + D+I Y ++ G +++ R + A+E ++ + + N+ + + AL++ Q + +N++I + R +A D F G++PN T+ ++ I G ++ LF M +GCT D + +V L Q + A + L ++ ++
Sbjct: 82 EVTPDLCTYGILIGCCCRAGRLDLGFAALGNVIKKGFRVDAIAFTPLLKGLCADKRTSDAMDIVLRRMTELGCIPNVFSYNILLKGLCDENRSQEALELLHMMADDRGGGSPPDVVSYTTVINGFFKEGDSDKAYSTYHEMLDRGILPDVVTYNSIIAALCKAQAMDKAMEVLNTMVKNGVMPDCMTYNSILHGYCSSGQPKEAIGFLKKMRSDGVEPDVVTYSLLMDYLCKNGRCMEARKIFDSMTKRGLKPEITTYGTLLQGYATKGALVEMHGLLDLMVRNGIHPDHYVFSILICAYAKQGKVDQAMLVFSKMRQQGLNPNAVTYGAVIGILCKSGRVEDAMLYFEQMIDEGLSPGNIVYNSLIHGLCTCNKWERAEELILEMLDRGICLNTIFFNSIIDSHCKEGRVIESEKLFELMVRIGVKPNVITYNTLINGYCLAGKMDEAMKLLSGMVSVGLKPNTVTYSTLINGYCKISRMEDALVLFKEMESSGVSPDIITYNIILQGLFQT-----------RRTAAAKELYVRITESGTQIELSTYNIILHGLCKNKLTDDALQMFQNLCLMDLKLEARTFNIMIDALLKVGRNDEAKDLFVAFSSNGLVPNYWTYRLMAENIIGQGLLEELDQLFLSMEDNGCTVDSGMLNFIVRELLQRGEITRAGTYLSMIDEK 750 HSP 4 Score: 192.971 bits (489), Expect = 4.195e-49 Identity = 174/756 (23.02%), Postives = 329/756 (43.52%), Query Frame = -2
Query: 1905 EQLINRGLLSSARKLFQRV--VSNSSPVSDALSTVDFVTSRGLDL---DLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEKPAF--NALVRLLCSQERFLDA--------------------------------------FDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLM------KMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVI----LIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERD---LIFYISLVSG-----FYRSISSRKRWF 3983
++L+ RG +S L + + V+ SP + A+S + + G D DL TY +LI ++G L L ++ + +I +G D+ ++ LC + +A + R +T+ C F N L++ LC + R +A + + +M++ + YN +I LC+ +++A+++ + M + +P Y S+ +G C G EA KM S G D Y+ L++ CK + A +++ M K G +P+ TY TL+ G+ G L + M+ G+ P F ++I Y ++GKVD A ++ + M + L PNA Y +I L K R+ +A ++E+M++ GL P ++++ L+ + + EL I M+ + I LN F+ ++ + ++ VI L E +++ + + +N LI+ C + D A + M S+G P TY++LI + EDA L M++ G+ PD T II+ + A ++ ++ + G + ++ YN I+ L + K +A MF + + + + MI+ GR EA LF + P +Y + ++ + + ++ M D+G ++ + ++ LQ+ E A + ++D + +I L+SG +YR + + + F
Sbjct: 39 DELLRRGRGASIYGLNRALADVARDSPAA-AVSRYNRMARAGADEVTPDLCTYGILIGCCCRAGRLDLGFAALGN-VIKKGFRVDAIAFTPLLKGLCADKRTSDAMDIVLRRMTELGCIPNVFSYNILLKGLCDENRSQEALELLHMMADDRGGGSPPDVVSYTTVINGFFKEGDSDKAYSTYHEMLDRGILPDVVTYNSIIAALCKAQAMDKAMEVLNTMV-KNGVMPDCMTYNSILHGYCSSGQPKEAIGFLKKMRSDGVEPDVVTYSLLMDYLCKNGRCMEARKIFDSMTKRGLKPEITTYGTLLQGYATKGALVEMHGLLDLMVRNGIHPDHYVFSILICAYAKQGKVDQAMLVFSKMRQQGLNPNAVTYGAVIGILCKSGRVEDAMLYFEQMIDEGLSPGNIVYNSLIHGLCTCNKWERAEEL-ILEMLDRGICLNTIFFNSII-----DSHCKEGRVIESEKLFELMVRIGVKPNVITYNTLINGYCLAGKMDEAMKLLSGMVSVGLKPNTVTYSTLINGYCKISRMEDALVLFKEMESSGVSPDIITYNIILQGLFQTRRTAAAKELYVRITESGTQIELSTYNIILHGLCKNKLTDDALQMFQNLCLMDLKLEARTFNIMIDALLKVGRNDEAKDLFVAFSSNGLVPNYWTYRLMAENIIGQGLLEELDQLFLSMEDNGCTVDSGMLNFIVRELLQRGEITRAGTYLSMIDEKHFSLEASTASLFIDLLSGGKYQEYYRFLPEKYKSF 785 HSP 5 Score: 152.525 bits (384), Expect = 3.019e-36 Identity = 125/541 (23.11%), Postives = 237/541 (43.81%), Query Frame = -2
Query: 1167 LIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGC---MPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDI-LDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLE---SGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALK--------LIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTFRK 2744
+ +E+L+ + N ++ + + + S + ++M G P L TY LI C + G + + L + +G D ++ C A DI L +M + G P V YN ++ L + + EA + M + G PD + Y T+INGF G +A +HEM+ I P +Y ++I+ L K DK L M+ +G++P+ + Y S++ + + + A + M + +E D++ Y L+ + + G K+F + ++ L P+ + ++ +A K L+ + + P+ Y+++++I + + ++ A F M+++G+ PN VT+ ++G K+G ++ A+ F +M +G +P IVY L++GLC ++ A L+ M RG+ + + I+ C + + K+FE MV P + N L+ C+ K+ EA + ++ G P T S I + K
Sbjct: 37 VFDELLRRGRGASIYGLNRALADVARDSPAAAVSRY-NRMARAGADEVTPDLCTYGILIGCCCRAGRLDLGFAALGNVIKKGFRVDAIAFTPLLKGLCADKRTSDAMDIVLRRMTELGCIPNVFSYNILLKGLCDENRSQEALELLHMMADDRGGGSPPDVVSYTTVINGFFKEGDSDKAYSTYHEMLDRGILPDVVTYNSIIAALCKAQAMDKAMEVLNTMVKNGVMPDCMTYNSILHGYCSSGQPKEAIGFLKKMRSDGVEPDVVTYSLLMD------------YLCKNGRCMEARKIFDSMTKRGLKPE------ITTYGTLLQGYATKGALVEMHGLLDLMVRNGIHPDHYVFSILICAYAKQGKVDQAMLVFSKMRQQGLNPNAVTYGAVIGILCKSGRVEDAMLYFEQMIDEGLSPGNIVYNSLIHGLCTCNKWERAEELILEMLDRGICLNTIFFNSIIDSHCKEGRVIESEKLFELMVRIGVKPNVITYNTLINGYCLAGKMDEAMKLLSGMVSVGLKPNTVTYSTLINGYCK 558
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy Swiss-Prot
Match: PP247_ARATH (Pentatricopeptide repeat-containing protein At3g22470, mitochondrial OS=Arabidopsis thaliana GN=At3g22470 PE=2 SV=1) HSP 1 Score: 222.246 bits (565), Expect = 2.006e-59 Identity = 135/489 (27.61%), Postives = 225/489 (46.01%), Query Frame = -2
Query: 2157 LVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALI 3623
++ C +++ L AF + + ++ L++G C +G + EA+ + D M E + P L +L GLC +G V EA L +M GF D+ Y ++N CK +AL ++ +M + + Y+ +I K G FD LFN+M +G++ VVT+ +I C +GK D + +L MI +N+ P+ ++ LI KE ++ EA+E Y M+ G+ PD + + L+ + K L A + + GC D V +++LI++ C+ + D ++ S G +P TYN+L+ Q G A+ L M ++G+ P T I+++ C +G L A +I ++M+ M G+ IYN II + KV +A ++F + + GV PD + Y MI G G + EAD LF +M P +Y LI
Sbjct: 111 MINCYCRKKKLLFAFSVLGRAWKLGYEPDTITFSTLVNGFCLEGRVSEAVALVDRMVEMKQR-PDLVTVSTLINGLCLKGRVSEALVLIDRMVEYGFQPDEVTYGPVLNRLCKSGNSALALDLFRKMEERNIKASVVQYSIVIDSLCKDGSFDDALSLFNEMEMKGIKADVVTYSSLIGGLCNDGKWDDGAKMLREMIGRNIIPDVVTFSALIDVFVKEGKLLEAKELYNEMITRGIAPDTITYNSLIDGFCKENCLHEANQMFDLMVSKGCEPDI-------------------------------VTYSILINSYCKAKRVDDGMRLFREISSKGLIPNTITYNTLVLGFCQSGKLNAAKELFQEMVSRGVPPSVVTYGILLDGLCDNGELNKALEIFEKMQKSRMTLGIGIYNIIIHGMCNASKVDDAWSLFCSLSDKGVKPDVVTYNVMIGGLCKKGSLSEADMLFRKMKEDGCTPDDFTYNILI 567 HSP 2 Score: 220.705 bits (561), Expect = 6.986e-59 Identity = 146/561 (26.02%), Postives = 260/561 (46.35%), Query Frame = -2
Query: 1368 RITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLA----KVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGF------------YRSISSRKRWFAM-----------RRGSERAREKLFQLLHRQSLLPK--EKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYL 2963
++ +A + +E M+ +P + F +L + + D+ K + LNG D + + N +K ++ +L L + F+ L++ C + + A +D+M + P L T ++LI L KG +A L++RM G PD+ T ++N CK GN A D+ +ME+R +K V Y+ +I SL + +A ++F M G+ D + Y ++I G N+G+ + ++ EMIG I P +++ALI VK+ + +M+ G+ P+ + Y SLI F ++ A ++ DLM E D++ Y L++ + +R ISS+ + G A ++LFQ + + + P + + AL++ +K++++R + +YN+II G C A ++ DA+ F + +GV P+ VT+ +++GG K G + A LF KM DGCTPD Y IL+ S L+ ++ L+ M G T ++++ D L
Sbjct: 50 KVNDAIDLFESMIQSRPLPTPIDFNRLCSAVARTKQYDLVLGFCKGMELNGIEHDMYTMTIMINCYCRKKKLLFAFSVLGRAWKLGYEPDTITFSTLVNGFCLEGRVSEAVALVDRMVEMKQRPDLVTVSTLINGLCLKGRVSEALVLIDRMVEYGFQPDEVTYGPVLNRLCKSGNSALALDLFRKMEERNIKASVVQYSIVIDSLCKDGSFDDALSLFNEMEMKGIKADVVTYSSLIGGLCNDGKWDDGAKMLREMIGRNIIPDVVTFSALIDVFVKEGKLLEAKELYNEMITRGIAPDTITYNSLIDGFCKENCLHEANQMFDLMVSKGCEPDIVTYSILINSYCKAKRVDDGMRLFREISSKGLIPNTITYNTLVLGFCQSGKLNAAKELFQEMVSRGVPPSVVTYGILLDGLCDNGELNKALEIFEKMQKSRMTLGIGIYNIIIHGMCNASKVDDAWSLFCSLSDKGVKPDVVTYNVMIGGLCKKGSLSEADMLFRKMKEDGCTPDDFTYNILIRAHLGGSGLISSVELIEEMKVCGFSADSSTIKMVIDMLSDRRL 610 HSP 3 Score: 187.963 bits (476), Expect = 4.932e-48 Identity = 126/497 (25.35%), Postives = 229/497 (46.08%), Query Frame = -2
Query: 2412 ALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDN-SCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQME 3899
A S + G + D T++ L+ G + A + D ++ PD ++++ LC G++ EA +L DR++ ++ + ++ LC A D F KM N+ Y+++ID LC+ G ++A+ +F+ M + + Y SL GLC G + + +M + D +++LI+V+ K K+ A +Y M+ G PD+ TYN+LI GF K +F+ M+ +G +P +VT+ ++I++YC+ +VD L + SK L PN Y L+ + ++ A+E ++ M++ G+ P V + I L+G + N EL + + I E++ KS + L +N++I +C ++ D A + G P + TYN +I L +KG +A+ L +M+ G PD T I+I H L + +++++M+
Sbjct: 124 AFSVLGRAWKLGYEPDTITFSTLVNGFCLEGRVSEAVALV-DRMVEMKQRPDLVTVSTLINGLCLKGRVSEALVLIDRMVEYGFQPDEVTYGPVLNRLCKSGNSALALDLFRKMEERNIKASVVQYSIVIDSLCKDGSFDDALSLFNEMEMKGIKADVVT-YSSLIGGLCNDGKWDDGAKMLREMIGRNIIPDVVTFSALIDVFVKEGKLLEAKELYNEMITRGIAPDTITYNSLIDGFCKENCLHEANQMFDLMVSKGCEPDIVTYSILINSYCKAKRVDDGMRLFREISSKGLIPNTITYNTLVLGFCQSGKLNAAKELFQEMVSRGVPPSVVTY---------------------GILLDG---------LCDNGELNKALEIF-EKMQKSRMTLGIGIYNIIIHGMCNASKVDDAWSLFCSLSDKGVKPDVVTYNVMIGGLCKKGSLSEADMLFRKMKEDGCTPDDFTYNILIRAHLGGSGLISSVELIEEMK 587 HSP 4 Score: 171.785 bits (434), Expect = 8.182e-43 Identity = 126/554 (22.74%), Postives = 232/554 (41.88%), Query Frame = -2
Query: 1827 LEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKE 3488
+ +AI +F+ M + + LPT + L + + ME G D T +IN YC+ +K+ A V R K+G PD+ T++TL++GF G L ++M+E +P +VT +I+ C +G+V A +L++ M+ P+ Y ++ L K A + + +M + V + ++ K D A + + + G D V ++ LI LC + D + + +M +P + T+++LI ++G +A+ L N M +GI PD T +I+ CK L A + D M +G +P + Y+ +I S + K+V + +F + G+ P+ I Y T++ GF +G++ A LF EM+ + P+ +Y L+ GL +K EKM + +Y +I + + A+ L + ++ D++ Y ++ G ++GS + LF+ + P +
Sbjct: 51 VNDAIDLFESMIQ-SRPLPTPIDFNRLCSAVARTKQYDLVLGFCKGMELNGIEHDMYTMTIMINCYCRKKKLLFAFSVLGRAWKLGYEPDTITFSTLVNGFCLEGRVSEAVALVDRMVEMKQRPDLVTVSTLINGLCLKGRVSEALVLIDRMVEYGFQPDEVTYGPVLNRLCKSGNSALALDLFRKMEERNIKASVVQYSIVIDSLCKDGSFDDALSLFNEMEMKGIKADV-------------------------------VTYSSLIGGLCNDGKWDDGAKMLREMIGRNIIPDVVTFSALIDVFVKEGKLLEAKELYNEMITRGIAPDTITYNSLIDGFCKENCLHEANQMFDLMVSKGCEPDIVTYSILINSYCKAKRVDDGMRLFREISSKGLIPNTITYNTLVLGFCQSGKLNAAKELFQEMVSRGVPPSVVTYGILLDGLCDNGELNKALEIFEKMQKSRMTLGIGIYNIIIHGMCNASKVDDAWSLFCSLSDKGVKPDVVTYNVMIGGL------------CKKGSLSEADMLFRKMKEDGCTPDD 560 HSP 5 Score: 168.318 bits (425), Expect = 9.383e-42 Identity = 137/604 (22.68%), Postives = 250/604 (41.39%), Query Frame = -2
Query: 1488 KMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMK-MYPKGYELD----IAFMVLKAIALNGCGFDPLL--LPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGL 3278
K+ A+ ++ M++ P +N L + +D M G++ + T +MI+ YCR+ K+ A +L P+ ++ L+ E R++EA +RM+ PD V L+ + KG + I MV + + P+L L S N L + +EE N+ + V ++++I +LC+ D A ++ME G + TY+SLI L G ++D +L M + I PD T +I+ K G L A ++ ++M RG+ P YN +I ++ + EA MF M+ G +PD + Y +IN + RV + RLF E+ + P + +Y L+ G + + ++M+ G+ P+ V Y L+ E A + + M ++++ + Y ++ G + W LF L + + P++ YNV+I G C+ + +A F M+++G P+ T+ IL+ H+ + ++ L +M G + D K++++ L
Sbjct: 50 KVNDAIDLFESMIQSRPLPTPIDFNRLCSAVARTKQYDLVLGFCKGMELNGIEHDMYTMTIMINCYCRKKKLLFAFSVLGRAWKLGYEPDTITFSTLVNGFCLEGRVSEAVALVDRMVEMKQRPDLVTVSTLINGLCLKGRVSEALVLIDRMVEYGFQPDEVTYGPVLNRLCKSGNSALALDLFRKMEE---RNIKASVVQYSIVIDSLCKDGSFDDALSLFNEMEMKGIKADVVTYSSLIGGLCNDGKWDDGAKMLREMIGRNIIPDVVTFSALIDVFVKEGKLLEAKELYNEMITRGIAPDTITYNSLIDGFCKENCLHEANQMFDLMVSKGCEPDIVTYSILINSYCKAKRVDDGMRLFREISSKGLIPNTITYNTLVLGFCQSGKLNAAKELFQEMVSRGVPPSVVTYGILLDGLCDNGELNKALEIFEKMQKSRMTLGIGIYNIIIHGMCNASKVDDAW------------SLFCSLSDKGV---------------------------------KPDVVTYNVMIGGLCKKGSLSEADMLFRKMKEDGCTPDDFTYNILIRAHLGGSGLISSVELIEEMKVCGFSADSSTIKMVIDML 605 HSP 6 Score: 147.902 bits (372), Expect = 2.966e-35 Identity = 110/406 (27.09%), Postives = 191/406 (47.04%), Query Frame = -2
Query: 2802 VSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVI-CLCKLGKLEEATILFDRL-LTDNSCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTF-----------------------------------HVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGF 3908
VS+AL +D + G D TY ++ +L +SG+ LA + + I S + SIVI LCK G ++A LF+ + + + +++L+ LC+ ++ D +MI N+ ++ LID ++G L EA ++++ M R + P Y SL G CK+ + EA +F M S+G D Y+ LIN YCK +++ +R++ + G P++ TYNTL+ GF + G + LF +M+ +G+ PSVVT+ +++I C KVD A L ++ K + P+ Y V+I L K+ ++EA+ + +M G PD + L++ + G L + +++ + + CGF
Sbjct: 191 VSEALVLIDRMVEYGFQPDEVTYGPVLNRLCKSGNSALALDLFRK--MEERNIKASVVQYSIVIDSLCKDGSFDDALSLFNEMEMKGIKADVVTYSSLIGGLCNDGKWDDGAKMLREMIGRNIIPDVVTFSALIDVFVKEGKLLEAKELYNEMITRGIA-PDTITYNSLIDGFCKENCLHEANQMFDLMVSKGCEPDIVTYSILINSYCKAKRVDDGMRLFREISSKGLIPNTITYNTLVLGFCQSGKLNAAKELFQEMVSRGVPPSVVTYGILLDGLCDNGELNKALEIFEKMQKSRMTLGIGIYNIIIHGMCNASKVDDAWSLFCSLSDKGVKPDVVTYNVMIGGLCKKGSLSEADMLFRKMKEDGCTPDDFTYNILIRAHLGGSGLISSVELIEEMKV--CGF 591 HSP 7 Score: 135.191 bits (339), Expect = 2.894e-31 Identity = 89/334 (26.65%), Postives = 157/334 (47.01%), Query Frame = -2
Query: 2904 DALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDN-SCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPD 3902
DALS + + +G+ D+ TY+ LI L G + +IGR IIPD ++++ K GKL EA L++ ++T + + +N+L+ C + +A F M++ Y++LI+ C+ +++ +++F + + +P Y +L G C+ G + A+ LF +M S+G Y L++ C ++ AL ++ +M K YN +IHG D W LF + ++G++P VVT++VMI C++G + A ML M P+ Y +LI + + + + E E M G D
Sbjct: 263 DALSLFNEMEMKGIKADVVTYSSLIGGLCNDGKWDDGAKMLRE-MIGRNIIPDVVTFSALIDVFVKEGKLLEAKELYNEMITRGIAPDTITYNSLIDGFCKENCLHEANQMFDLMVSKGCEPDIVTYSILINSYCKAKRVDDGMRLFREISSKG-LIPNTITYNTLVLGFCQSGKLNAAKELFQEMVSRGVPPSVVTYGILLDGLCDNGELNKALEIFEKMQKSRMTLGIGIYNIIIHGMCNASKVDDAWSLFCSLSDKGVKPDVVTYNVMIGGLCKKGSLSEADMLFRKMKEDGCTPDDFTYNILIRAHLGGSGLISSVELIEEMKVCGFSAD 594 HSP 8 Score: 104.375 bits (259), Expect = 2.376e-21 Identity = 87/367 (23.71%), Postives = 161/367 (43.87%), Query Frame = -2
Query: 1167 RVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAR--EKLFQLLHRQSLLPKEKNL-------RVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTFRK 2240
+V +A LF MI + PT + L S + + D + + M +G+ + T +I+ + +K++ FAF ++ + E D I + +LV+GF R SE +++ ++ R L+ + RVS+ AL LI ++ + F P+ Y +++ C++ A D F M++ + + V ++I++ K G D A+ LFN+M G D + Y L+ GLC + + +L M R +IP T+ ++ F + A +++ EM+ P N L+ C + L EA ++D ++ +G P T S+ I ++ K
Sbjct: 50 KVNDAIDLFESMIQSRPLPTPIDFNRLCSAVARTKQYDLVLGFCKGMELNGIEHDMYTMTIMINCYCRKKKLLFAFSVLGRAWKLGYEPDTITFSTLVNGFCLE----------GRVSEAVALVDRMVEMKQRPDLVTVSTLINGLCLKGRVSE---------ALVLIDRMVEYGFQPDEVTYGPVLNRLCKSGNSALALDLFRKMEERNIKASVVQYSIVIDSLCKDGSFDDALSLFNEMEMKGIKADVVTYSSLIGGLCNDGKWDDGAKMLREMIGRNIIPDVVTFSALIDVFVKEGKLLEAKELYNEMITRGIAPDTITYNSLIDGFCKENCLHEANQMFDLMVSKGCEPDIVTYSILINSYCK 397 HSP 9 Score: 93.2041 bits (230), Expect = 7.326e-18 Identity = 63/233 (27.04%), Postives = 112/233 (48.07%), Query Frame = -2
Query: 3195 DFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEKPA---FNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLI 3884
D + S+G + D+ TY++LI ++ + + + I +G+IP++ N++V+ C+ GKL A LF +++ P+ + L+ LC A + F KM + LG YN++I G+C +++A +F + ++ P + Y + GLCK+G + EA+ LF KM+ G D Y LI + G + ++ + M G DS T +I
Sbjct: 374 DLMVSKGCEPDIVTYSILINSYCKAKRVDDGMRLFRE-ISSKGLIPNTITYNTLVLGFCQSGKLNAAKELFQEMVSRGV--PPSVVTYGILLDGLCDNGELNKALEIFEKMQKSRMTLGIGIYNIIIHGMCNASKVDDAWSLFCSLSDKGVK-PDVVTYNVMIGGLCKKGSLSEADMLFRKMKEDGCTPDDFTYNILIRAHLGGSGLISSVELIEEMKVCGFSADSSTIKMVI 602 HSP 10 Score: 68.1662 bits (165), Expect = 3.770e-10 Identity = 53/211 (25.12%), Postives = 94/211 (44.55%), Query Frame = -2
Query: 3279 VSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDN-SCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGR 3908
V D + ++S+GL + TY L+ QSG L A + + ++ RG+ P ++ LC G+L +A +F+++ + +N ++ +C+ + DA+ F + + V YNV+I GLC+KG L EA +F M E P Y L ++ + L +M+ GF D + +I++ R
Sbjct: 401 VDDGMRLFREISSKGLIPNTITYNTLVLGFCQSGKLNAAKELFQE-MVSRGVPPSVVTYGILLDGLCDNGELNKALEIFEKMQKSRMTLGIGIYNIIIHGMCNASKVDDAWSLFCSLSDKGVKPDVVTYNVMIGGLCKKGSLSEADMLFRKMKEDG-CTPDDFTYNILIRAHLGGSGLISSVELIEEMKVCGFSADSSTIKMVIDMLSDRR 609
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy TrEMBL
Match: A0A0B0MFC3_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_22354 PE=4 SV=1) HSP 1 Score: 1873.98 bits (4853), Expect = 0.000e+0 Identity = 909/932 (97.53%), Postives = 918/932 (98.50%), Query Frame = -2
Query: 1335 MTNKRVLSRHLFFKSRRAVTTSAALPLDPSYATISSIPADHFSLCLSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEM 4130
MTNKRVLSRHLFFK+RRAVTTSAALPLDPSYATISSIPADHFSLCLSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHL LAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRL+TDNSCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLC+QGWVVEAESLFGK+ESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLK GCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMM QGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNL PAFDILDQMEDRGMKPGVAIY+CII SLFR+KKVSEA MFVRML+SGVDPDEIIYLTMINGFSNNGRVIEA RLFHEMI AAIRPTSHSYTALISGLVKK+MTDKGC YLEKMLDDGLVPNAVLYTSLI+NFLQK EFEFAFRLVDLMDRNQIE DLI YISLVS FYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEM
Sbjct: 1 MTNKRVLSRHLFFKTRRAVTTSAALPLDPSYATISSIPADHFSLCLSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLLLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLVTDNSCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCRQGWVVEAESLFGKIESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKTGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMGQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLAPAFDILDQMEDRGMKPGVAIYDCIIRSLFRKKKVSEAKDMFVRMLKSGVDPDEIIYLTMINGFSNNGRVIEARRLFHEMIEAAIRPTSHSYTALISGLVKKDMTDKGCMYLEKMLDDGLVPNAVLYTSLINNFLQKGEFEFAFRLVDLMDRNQIELDLISYISLVSRFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEM 932 HSP 2 Score: 190.66 bits (483), Expect = 6.799e-46 Identity = 163/670 (24.33%), Postives = 299/670 (44.63%), Query Frame = -2
Query: 1173 QMMEQGLQPSVVT-FHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNG-GLVPDHVLFFKLMKMYPKGYELDIAFMVL-KAIALNGC---GFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKM-ESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERD-LIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHR--QSLLPKEK---NLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTF 3143
Q++ +GL S F ++SN V A ++ + S+ L + Y VLI L + + A FY + G G++PD + ++ K +L+ A ++ + + N C F+ L+ + + E I+ ++ N++L +NVLI LC++ + A D M E +P L Y SL L ++G +AESL ++++QG F D+ +IN +CK ++ A + +M G +P YN +I + +F +M+ G+ P + + MI+ + G+V A L + MI + P +H YT LI+ L K+N + + E+ML+ GLVP+ VL+ L+ + + E + AF ++ + N D L+ +S +E +K+ L+ +S L K N+ +S +A + A + K++ MP L+ YN +I + +DA MQ +G+ P+Q T I++ H K G + A + ++M G P +Y ++ L + ++ EA + M K G+ P + Y ++ F ++ + A ++F EM+ + P + L+ L + + C+ + ++ G P + I F
Sbjct: 51 QLINRGLLSSARKLFQRVVSN---SSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLLLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLVTDNSCEKPAFNALVRLLCSQERFLDAFDYFIK-MININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCRQGWVVEAESLFGKIESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKTGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMGQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVS--------------------ANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLAPAFDILDQMEDRGMKPGVAIYDCIIRSLFRKKKVSEAKDMFVRMLKSGVDPDEIIYLTMINGFSNNGRVIEARRLFHEMIEAAIRPTSHSYTALISGLVKKDMTDKGCMYLEKMLDDGLVPNAVLYTSLINNF 696 HSP 3 Score: 170.629 bits (431), Expect = 1.223e-39 Identity = 157/662 (23.72%), Postives = 285/662 (43.05%), Query Frame = -2
Query: 2067 QLINRGLLSSARKLFQRVVSN---SSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEKPAFNALVRLLCSQERFLDAFDYFIK-MININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVS--------------------ANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVL 3980
Q++ +GL S F ++SN V A ++ + S+ L + Y VLI L + + A FY + G G++PD + ++ K +L+ A ++ + + N C F+ L+ + + E I+ ++ N++L +NVLI LC++ + A D M E +P L Y SL L ++G +AESL +M++QG F D+ +IN +CK + A + +M G +P Y+ +I + +F +M++ G+ P + + MI+ + G+V A L + MI + P +H YT LI+ L K++ + + E+ML+ GLVP+ VL+ L+ + + E + AF ++ + N D L+ +S +E +K+ L+ +S L K N+ +S +A + A + K++ MP L+ YN +II+ C+ ++ A+D + M+ G+ P + ++G + ++ A +F +M G PD I+Y ++NG R++EA L H M + P+ +Y ++ + E+M DGL A+L
Sbjct: 330 QMMGQGLQPSVVT-FHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNG-GLVPDHVLFFKLMKMYPKGYELDIAFMVL-KAIALNGC---GFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKM-ESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLAPAFDILDQMEDRGMKPGVAIYDCIIRSLFRKKKVSEAKDMFVRMLKSGVDPDEIIYLTMINGFSNNGRVIEARRLFHEMIEAAIRPTSHSYTALISGLVKKDMTDKGCMYLEKMLDDGLVPNAVLYTSLINNFLQKGEFEFAFRLVDLMDRNQIELD-LISYISLVSRFYRSISSRKRWFAMRRGSERAREKLFQLLHR--QSLLPKEK---NLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYN-----------------------------------VIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEM-QDGLQLTAML 942 HSP 4 Score: 102.064 bits (253), Expect = 1.742e-18 Identity = 63/120 (52.50%), Postives = 64/120 (53.33%), Query Frame = -3
Query: 800 KDGLQLTAMLVLNY*SLSSVGFKNKERKISWLDASGVLLKTCQKVFFHKYRMRCCLCPEKSREKFKL*ADIRVCTSVL*VFQILQIPGSIELTGIKIKCSTNFPVFFQLVIWPNLMEKLI 1159
+DGLQLTAML RKISWLDASGVLLKTCQK ILQIPGSIELTGIKIKC TNFPVF QLVIWPNLMEKLI
Sbjct: 933 QDGLQLTAML----------------RKISWLDASGVLLKTCQK--------------------------------------ILQIPGSIELTGIKIKCYTNFPVFLQLVIWPNLMEKLI 998 HSP 5 Score: 65.855 bits (159), Expect = 2.651e-7 Identity = 48/197 (24.37%), Postives = 91/197 (46.19%), Query Frame = -2
Query: 1140 YLYNVIISGFCEADRMQDAYDHFELM-QKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTFRKW*ERWTSAD 1727
+ YNV+I G C+ +++A F+LM ++ LP + L G + G + A LF K+ + G D +Y L+N C+ ++ AL + + M K G P TY ++ F L + +F +M+ P + + ++ C + K+ A ++ +++I + P ++ I + K R T A+
Sbjct: 200 WYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCRQGWVVEAESLFGKIESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKTGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMGQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKE-NRITEAE 395
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy TrEMBL
Match: A0A0D2QJ46_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_003G114200 PE=4 SV=1) HSP 1 Score: 1848.56 bits (4787), Expect = 0.000e+0 Identity = 895/915 (97.81%), Postives = 904/915 (98.80%), Query Frame = -2
Query: 1398 MTNKRVLSRHLFFKSRRAVTTSAALPLDPSYATISSIPADHFSLCLSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSK----GTYEI 4130
MTNKRVLSRHLFFKSRRAVTTSAALPLDPSYAT+SSIPADHFSLCLSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRL+TDNSCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYN+LIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSL KENRI EAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFN+LISALCEQAQQDSA +FMDKMESLGCMPLLFTYNSLIKCLSQK LFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIY+CIIGSLFRQKKVSEATAMF+RMLESGVDPDEIIYLTMINGFSNNGRVIEAD+LFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWF+MRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYN IISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSK TY I
Sbjct: 1 MTNKRVLSRHLFFKSRRAVTTSAALPLDPSYATVSSIPADHFSLCLSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLVTDNSCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNMLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLCKENRIMEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNILISALCEQAQQDSALHFMDKMESLGCMPLLFTYNSLIKCLSQKSLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYDCIIGSLFRQKKVSEATAMFIRMLESGVDPDEIIYLTMINGFSNNGRVIEADQLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFSMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNGIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKEIAGSTYSI 915 HSP 2 Score: 195.667 bits (496), Expect = 1.115e-47 Identity = 164/670 (24.48%), Postives = 302/670 (45.07%), Query Frame = -2
Query: 1173 QMMEQGLQPSVVT-FHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNG-GLVPDHVLFFKLMKMYPKGYELDIAFMVL-KAIALNGC---GFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKM-ESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERD-LIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHR--QSLLPKEK---NLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTF 3143
Q++ +GL S F ++SN V A ++ + S+ L + Y VLI L + + A FY + G G++PD + ++ K +L+ A ++ + + N C F+ L+ + + E I+ ++ N++L +N+LI LC++ + A D M E +P L Y SL L ++G +AESL +M++QG F D+ +IN +CK ++ A + +M G +P YN +I + +F +M+E G+ P + + MI+ + G+V A L + MI + P +H YT LI+ L K+N + + E+ML+ GLVP+ VL+ L+ + + E + AF ++ + N D L+ +S +E +K+ L+ +S L K N+ +S +A + AL + K++ MP L+ YN +I + +DA MQ +G+ P+Q T I++ H K G ++ A + ++M G P +Y ++ L + ++ EA ++ M + G+ P + Y ++ F ++ + A ++F EM+ + P + L+ L + + C + ++ G P + I F
Sbjct: 51 QLINRGLLSSARKLFQRVVSN---SSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLVTDNSCEKPAFNALVRLLCSQERFLDAFDYFIK-MININVNLGCWYYNMLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLCKENRIMEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVS--------------------ANEELEQKIVILIEEILKSNLHLAKVAFNILISALCEQAQQDSALHFMDKMESLGCMPLLFTYNSLIKCLSQKSLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYDCIIGSLFRQKKVSEATAMFIRMLESGVDPDEIIYLTMINGFSNNGRVIEADQLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNF 696 HSP 3 Score: 138.272 bits (347), Expect = 1.174e-29 Identity = 148/689 (21.48%), Postives = 289/689 (41.94%), Query Frame = -2
Query: 1335 VVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYR-MLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGL--QPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPK-GYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGL-VKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKN--LRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEM 3380
V +A S + S+G +D + Y LI + + +A Y ++ G PDS N+++ K+G + +LF++++ +P+ F+ ++ C + + A MI+ N+ Y +LI L ++ + EA + ++ L+P+ + +Y Y L V++A +L G ++ + K + LI+ C+ + A +M +GC P +TYN+LI + GLF+ L N+M QG+ P T ++I+ +C+ G ++ A +L+ M + + P Y +I SL ++ ++ EA + RML G+ PD +++ ++ + E D F M+ AI + L+ + + + K +E++L L V + LIS ++ + + A +D M+ L Y SL+ S+K F E A E L + Q + P + + +++ A ++ +++ P + +Y+ II ++ +A F M + GV P+++ + ++ G G + A LF++M P Y L++GL + + + + L M GL+P+ Y ++ F AF++ + M
Sbjct: 75 VSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLVTDNSCEKPA---FNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNMLIDGLCQKGYLEEAIQMFD------LMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFG-------------------------KMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLCKENRIMEAEEFYERMLNGGLVPDHVLFFKLMKMYPKG---YELDIAF--MVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNILISALCEQAQQDSALHFMDKMESLGCMPLLFTYNSLIKCL-----SQKSLF------EDA-ESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYDCIIGSLFRQKKVSEATAMFIRMLESGVDPDEIIYLTMINGFSNNGRVIEADQLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLM 712
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy TrEMBL
Match: A0A061G037_THECC (Tetratricopeptide repeat-like superfamily protein, putative isoform 1 OS=Theobroma cacao GN=TCM_014940 PE=4 SV=1) HSP 1 Score: 1536.55 bits (3977), Expect = 0.000e+0 Identity = 739/988 (74.80%), Postives = 844/988 (85.43%), Query Frame = -2
Query: 1167 MTNKRVLSRHLFFKSRRAVTTSAALPLDPSYATISSIPADHFSLCLSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTFRK 4130
M KR+LS HLFFK+RRA+TTS LPLDPS+A +SSI DH S CLS +EQLI RGLLSSA++L QR++S SS VSDA++ VDFVT+RGLDLDLST+ LIKKLV+SG+ LAYS YSD II RGI PD I NS+VICLCKLGKLEEA+ LFDRLL +NS EKPAFNALVR L +QERFLD FDYF+ M +I VNLGCWYYN LIDGLCQKG LEEAIQMFDLM E PTLHLYKSLFYGLCK GWV+EAE L G++ESQGF+VD+TMYTSLI YCK RKMKMA+R+Y RMLK GC PDSYTYNTLIHGFVKMGLFD GWVL+NQMME+GLQP V+T+HVMISNYCREGK +CASMLLN+M+S NLAP+ HCYTVLITS YKENR+ EA E Y+ ML GG+VPDHVLFF LMKMYPKGYEL +A M+++AIA+NGCGFDPLLL VS +E+LEQKI +LI +I K+NL LA VAF +LISAL E + D+A +FMDK+ +LGCMPLLFTYNSL+KCLSQ+GLFEDA+SL++ MQ +GIFPDQAT LI++NEHCKHG+L AFDILDQMEDRGMKPGVAIY+CIIGSL RQK++ EA MF+RMLESG DPDEI+Y+TMING++ NGR+IEA +LF +MI AIRPTSHSYTALISGLVKK+MTDKGC YL++ML DGLVPN VLYTSLI+NFL+K EFEFAFRLVDLMDRNQIE DLI YI+LVSG R+I+SRKRW +++R SERARE LF+LLH + LLP+EK LRVSDSSPEAMKCFALKL+QKVK+TRFMPNLYLYN IISGFC ADRMQDAYDHFELMQKEGV PNQVT TILMGGHIKAGEIDHAI LFNKMNAD CTPD I Y L+ GLCQA RLLEALSLLH MHKRGLIP K TYE +L +FC SYL +PAFKIFEEM+ + P+ Y NWLLCILC Q+KLREA IV+D++IQRGKYP K T+ L +T RK
Sbjct: 1 MIKKRLLSCHLFFKTRRAITTST-LPLDPSFAAVSSICTDHKSFCLSLTEQLIKRGLLSSAQQLIQRIISQSSSVSDAITAVDFVTARGLDLDLSTFGALIKKLVRSGYPQLAYSLYSDNIIRRGINPDPFIVNSMVICLCKLGKLEEASTLFDRLLMNNSSEKPAFNALVRELFAQERFLDVFDYFVAMSDIGVNLGCWYYNGLIDGLCQKGNLEEAIQMFDLMRETAGLSPTLHLYKSLFYGLCKHGWVLEAEFLIGEIESQGFYVDRTMYTSLIKEYCKDRKMKMAMRIYLRMLKTGCEPDSYTYNTLIHGFVKMGLFDQGWVLYNQMMEKGLQPDVITYHVMISNYCREGKANCASMLLNSMVSNNLAPSVHCYTVLITSFYKENRLMEAGELYKSMLTGGIVPDHVLFFTLMKMYPKGYELHLALMIVQAIAVNGCGFDPLLLAVSDSEDLEQKIELLIGKIEKTNLSLANVAFTILISALSEGRKLDTAVHFMDKLMNLGCMPLLFTYNSLVKCLSQEGLFEDAKSLVDLMQDRGIFPDQATYLIMVNEHCKHGDLASAFDILDQMEDRGMKPGVAIYDCIIGSLCRQKRLFEAEDMFIRMLESGEDPDEIVYMTMINGYAKNGRLIEARQLFEKMIEDAIRPTSHSYTALISGLVKKDMTDKGCMYLDRMLGDGLVPNVVLYTSLINNFLRKGEFEFAFRLVDLMDRNQIEHDLITYIALVSGVCRNITSRKRWCSIKRSSERAREMLFRLLHYRCLLPREKKLRVSDSSPEAMKCFALKLMQKVKETRFMPNLYLYNGIISGFCWADRMQDAYDHFELMQKEGVRPNQVTLTILMGGHIKAGEIDHAIDLFNKMNADDCTPDKIAYNTLIKGLCQAGRLLEALSLLHAMHKRGLIPRKATYENLLAHFCASYLCIPAFKIFEEMLASNVVPRPYSYNWLLCILCEQKKLREAYIVFDTMIQRGKYPLKSTERLLAETLRK 987 HSP 2 Score: 186.808 bits (473), Expect = 1.167e-44 Identity = 156/642 (24.30%), Postives = 279/642 (43.46%), Query Frame = -2
Query: 1998 VTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEKPAFNALVRLLCSQERFLDAFDYFI-KMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFD-----PLLLPVSANEELEQKIVIL------IEEILKSNLH---LAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDL 3878
+ S L + Y VLI + L A Y + G GI+PD + +++ K +L A ++ + N C F+ L+ + E + I K+ N++L + +LI L + L+ A+ D + +P L Y SL L ++G +A+SL M+ +G F D+ Y ++N +CK + A + +M G +P Y+ +I + +F +M+E G P + + MI+ Y + G++ A L MI + P +H YT LI+ L K++ + + +RML GLVP+ VL+ L+ + + E + AF ++ + N D L+ V N ++ + E+L LH L + +S +A + A M K++ MP L+ YN +I +DA MQ +G+ P+Q T I++ H K G ++ A D+ ++M P YN +I L + ++ EA ++ M + G+ P + Y ++ F + I A ++F EM+ + + P +SY L+ L ++ + + M+ G P L ++ E +F F + D+
Sbjct: 365 MVSNNLAPSVHCYTVLITSFYKENRLMEAGELYKSMLTG-GIVPDHVLFFTLMKMYPKGYELHLALMIVQAIAV-NGC---GFDPLLLAVSDSEDLEQKIELLIGKIEKTNLSLANVAFTILISALSEGRKLDTAVHFMDKLMN-LGCMPLLFTYNSLVKCLSQEGLFEDAKSLVDLMQDRGIFPDQATYLIMVNEHCKHGDLASAFDILDQMEDRGMKPGVAIYDCIIGSLCRQKRLFEAEDMFIRMLESGEDPDEIVYMTMINGYAKNGRLIEARQLFEKMIEDAIRPTSHSYTALISGLVKKDMTDKGCMYLDRMLGDGLVPNVVLYTSLINNFLRKGEFEFAFRLVDLMDRNQIEHDLITYIALVSGVCRNITSRKRWCSIKRSSERAREMLFRLLHYRCLLPREKKLRVSDSSPEAMKCFALKLMQKVKETRFMPNLYLYNGIISGFCWADRMQDAYDHFELMQKEGVRPNQVTLTILMGGHIKAGEIDHAIDLFNKMNADDCTPDKIAYNTLIKGLCQAGRLLEALSLLHAMHKRGLIPRKATYENLLAHFCASYLCIPAFKIFEEMLASNVVPRPYSYNWLLCILCEQKKLREAYIVFDTMIQRGKYPLKSTERLLAETLRKQGECDFGFMIQDI 1000 HSP 3 Score: 181.8 bits (460), Expect = 4.163e-43 Identity = 178/816 (21.81%), Postives = 339/816 (41.54%), Query Frame = -2
Query: 1572 VTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDN-SCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLM------PERTESLPTLHLYKSLFYGLCKQGWVVEA-------------------------ESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRG--MKPGVAIYNCIIGSLFRQKKVS------EATAMFV-----RMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLR--VSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEID 3878
+ S+G +D + Y LIK+ + + +A Y ++ G PDS N+++ K+G ++ +L+++++ + ++ ++ C + + A M++ N+ Y VLI ++ L EA +++ M P+ + +Y Y L +V+A E L GK+E + +T LI+ +GRK+ A+ +++ +GC P +TYN+L+ + GLF+ L + M ++G+ P T+ +M++ +C+ G + A +L+ M + + P Y +I SL ++ R+ EAE+ + RML G PD +++ ++ Y K L + A + E+ +IE+ ++ H ++ LIS L ++ D ++D+M G +P + Y SLI +KG FE A L++ M I D T + +++ C++ + + + +R M + Y C+ L R+KK+ EA F ++ E+ P+ +Y +I+GF R+ +A F M +RP + T L+ G +K D KM D P+ + Y +LI Q A RL++ + L +H++ L+P++ ++ + A K+ +++ + +P Y YN ++ CE ++++AY F+ M + G P + T +L K GE D
Sbjct: 260 IESQGFYVDRTMYTSLIKEYCKDRKMKMAMRIYLR-MLKTGCEPDSYTYNTLIHGFVKMGLFDQGWVLYNQMMEKGLQPDVITYHVMISNYCREGKANCASMLLNSMVSNNLAPSVHCYTVLITSFYKENRLMEAGELYKSMLTGGIVPDHVLFFTLMKMYPK-GYELHLALMIVQAIAVNGCGFDPLLLAVSDSEDLEQKIELLIGKIEKTNLSLANVAFTILISALSEGRKLDTAVHFMDKLMNLGCMPLLFTYNSLVKCLSQEGLFEDAKSLVDLMQDRGIFPDQATYLIMVNEHCKHGDLASAFDILDQMEDRGMKPGVAIYDCIIGSLCRQKRLFEAEDMFIRMLESGEDPDEIVYMTMINGYAKNGRL-----------------------IEARQLFEK----MIEDAIRPTSH----SYTALISGLVKKDMTDKGCMYLDRMLGDGLVPNVVLYTSLINNFLRKGEFEFAFRLVDLMDRNQIEHDLITYIALVSGVCRNITSRKRWCSIKRSSERAREMLFRLLHYRCL---LPREKKLRVSDSSPEAMKCFALKLMQKVKETRFMPNLYLYNGIISGFCWADRMQDAYDHFELMQKEGVRPNQVTLTILMGGHIKAGEIDHAIDLFNKMNADDCTPDKIAYNTLIKGLCQ------AGRLLEAL-----------------------------------------SLLHAMHKRGLIPRKATYENLLAHFCASYLCIPAFKIFEEMLASNVVPRPYSYNWLLCILCEQKKLREAYIVFDTMIQRGKYPLKSTERLLAETLRKQGECD 992
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy TrEMBL
Match: F6HAK9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_16s0022g01780 PE=4 SV=1) HSP 1 Score: 1097.03 bits (2836), Expect = 0.000e+0 Identity = 542/929 (58.34%), Postives = 685/929 (73.74%), Query Frame = -2
Query: 1365 MTNKRVLSRHLFFKSRRAVTT-SAALPLDPSYATISSIPADHFSLCLSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCE-KPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSA----NEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISS-RKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLA 4130
M K + R LFF ++ + T S AL PS A + H LC + +++LI RG+LS +++ +R++ S VSDA+ V+F +RGL+LD Y VL++KLV SG A + Y DY+I RGIIPDS NS+VIC C LGKLEEA FDRL +S KPA NA++R LC++ER L+AFDYF+++ ++ + +G W +N LIDGLC KG+++EA MFD M ERT T+HLYK+LFYGLC+Q V EAE G+MES+G F+DK MYTSLI+ YC+G+KM+ A+RV+ RMLKMGC PD+YTYNTLIHGFVK+GLFD GW+L NQM E GLQP+VVT+H+MI YC EGKVDCA LL++M S NL P+ H YTVLIT+LYKENR+ E EE Y++ML+ G+VPDHVLFF LM+ PKG+EL +A +L+AIA NGC D LL SA +++EQ+I L+ EI++ N LA VAF + ISALC + D+A FMDKM SLGC PLL TYNSLIKCL Q+ L EDA+SL++ MQ GI PD AT LI+++EHC HG+L AF +LDQM +RG+KP VAIY+ IIG L R+K++ EA +F MLE+GVDPD IIY+TMI+G+S N R IEA +LF +MI +P+SHSYTA+ISGLVK+NM DKGC+YL ML DG VPN VLYTSLI+ FL+K E EFAFRLVDLMDRNQIE D+I I+LVSG R+I+ R+RW+ ++ GS R RE L LLH+ ++P+E NL SP +K FAL L+QK+K + FMPNLYLYN IISGFC A+ +QDAY+HFELMQ EGV PNQVTFTIL+ GH + GEIDHAIGLFNKMNADG PDGI Y L+ GLC+A RLL+ALS+ H MHKRGL P+K +YE +L+ C S+L
Sbjct: 1 MLKKGLKPRRLFFTPKKNLATCSPALDPPPSSAPTTE---HHNKLCFTLTDRLIRRGVLSLGQQVVRRMIKQSPSVSDAILAVEFAAARGLELDSCGYGVLLRKLVGSGEHRFAEAVYRDYVIARGIIPDSETLNSMVICYCNLGKLEEAMAHFDRLFEVDSFPCKPACNAMLRELCARERVLEAFDYFVRINDVGILMGLWCFNRLIDGLCDKGHVDEAFYMFDTMRERTGLPATIHLYKTLFYGLCRQERVEEAELFVGEMESEGHFIDKMMYTSLIHGYCRGKKMRTAMRVFLRMLKMGCDPDTYTYNTLIHGFVKLGLFDKGWILHNQMSEWGLQPNVVTYHIMIRRYCEEGKVDCALTLLSSMSSFNLTPSVHSYTVLITALYKENRLVEVEELYKKMLDIGVVPDHVLFFTLMQKQPKGHELHLALKILQAIAKNGCNLDLCLLSTSATHSPTQDVEQEIECLLGEIVRRNFALADVAFGIFISALCAAGKTDAALLFMDKMVSLGCRPLLSTYNSLIKCLFQERLVEDAKSLIDLMQENGIVPDLATYLIMVHEHCNHGDLASAFGLLDQMNERGLKPSVAIYDSIIGCLSRRKRILEAENVFKMMLEAGVDPDAIIYVTMISGYSKNRRAIEARQLFDKMIEHGFQPSSHSYTAVISGLVKENMIDKGCSYLSDMLKDGFVPNTVLYTSLINQFLRKGELEFAFRLVDLMDRNQIECDMITCIALVSGVSRNITPVRRRWYHVKSGSARVREILLHLLHQSFVIPRENNLSFPRGSPRKIKYFALNLMQKIKGSSFMPNLYLYNGIISGFCRANMIQDAYNHFELMQTEGVCPNQVTFTILINGHTRFGEIDHAIGLFNKMNADGLAPDGITYNALIKGLCKAGRLLDALSVSHTMHKRGLFPNKSSYEKLLKCLCASHLG 926 HSP 2 Score: 161.384 bits (407), Expect = 1.232e-36 Identity = 123/511 (24.07%), Postives = 226/511 (44.23%), Query Frame = -2
Query: 1173 FNVLISALCEQAQQDSASYFMDKM-ESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQ--SLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTF 2696
FN LI LC++ D A Y D M E G + Y +L L ++ E+AE + M+++G F D+ +I+ +C+ + A + +M G P YN +I + + + +M E G+ P+ + Y MI + G+V A L M + P+ HSYT LI+ L K+N + +KMLD G+VP+ VL+ +L+ + E A +++ + +N DL S + + E+ E L + R+ +L + +S AL + K+ P L YN +I + ++DA +LMQ+ G++P+ T+ I++ H G++ A GL ++MN G P +Y ++ L + R+LEA ++ +M + G+ P Y ++ + + A+ A ++F++M+ + P + ++ L + + + C +++ G P + I F
Sbjct: 201 FNRLIDGLCDKGHVDEAFYMFDTMRERTGLPATIHLYKTLFYGLCRQERVEEAELFVGEMESEGHFIDKMMYTSLIHGYCRGKKMRTAMRVFLRMLKMGCDPDTYTYNTLIHGFVKLGLFDKGWILHNQMSEWGLQPNVVTYHIMIRRYCEEGKVDCALTLLSSMSSFNLTPSVHSYTVLITALYKENRLVEVEELYKKMLDIGVVPDHVLFFTLMQKQPKGHELHLALKILQAIAKNGCNLDLCLL------------STSATHSPTQDVEQEIECLLGEIVRRNFALADVAFGIFISALCAAGKTDAALLFMDKMVSLGCRPLLSTYNSLIKCLFQERLVEDAKSLIDLMQENGIVPDLATYLIMVHEHCNHGDLASAFGLLDQMNERGLKPSVAIYDSIIGCLSRRKRILEAENVFKMMLEAGVDPDAIIYVTMISGYSKNRRAIEARQLFDKMIEHGFQPSSHSYTAVISGLVKENMIDKGCSYLSDMLKDGFVPNTVLYTSLINQF 699 HSP 3 Score: 138.272 bits (347), Expect = 1.715e-29 Identity = 127/558 (22.76%), Postives = 238/558 (42.65%), Query Frame = -2
Query: 1209 LDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDR-GMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFY---RSISSRKRWFAMRR-GSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPN----QVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYP 2855
L + F + + L+ CG+ LL + + E + + ++ + N ++ C + + A D++ + P N++++ L + +A R+ GI +I+ C G+++ AF + D M +R G+ + +Y + L RQ++V EA M G D+++Y ++I+G+ ++ A R+F M+ P +++Y LI G VK + DKG +M + GL PN V Y +I + ++ + + A L+ M + + Y L++ Y R + + + M G F L+ +Q PK L + ALK++Q + + +L L +S QD E + E V N V F I + AG+ D A+ +KM + GC P Y L+ L Q + +A SL+ +M + G++P TY I++ C+ AF + ++M P + + ++ L ++++ EA V+ +++ G P
Sbjct: 78 LAVEFAAARGLELDSCGYGVLLRKLVGSGEHRFAEAVYRDYVIARGIIPDSETLNSMVICYCNLGKLEEAMAHFDRLFEVDSFPCKPACNAMLRELCARERVLEAFDYFVRINDVGILMGLWCFNRLIDGLCDKGHVDEAFYMFDTMRERTGLPATIHLYKTLFYGLCRQERVEEAELFVGEMESEGHFIDKMMYTSLIHGYCRGKKMRTAMRVFLRMLKMGCDPDTYTYNTLIHGFVKLGLFDKGWILHNQMSEWGLQPNVVTYHIMIRRYCEEGKVDCALTLLSSMSSFNLTPSVHSYTVLITALYKENRLVEVEELYKKMLDIGVVPDHVLFFTLMQKQ---PKGHELHL-----------ALKILQAIAKNGCNLDLCL----LSTSATHSPTQDVEQEIECLLGEIVRRNFALADVAFGIFISALCAAGKTDAALLFMDKMVSLGCRPLLSTYNSLIKCLFQERLVEDAKSLIDLMQENGIVPDLATYLIMVHEHCNHGDLASAFGLLDQMNERGLKPSVAIYDSIIGCLSRRKRILEAENVFKMMLEAGVDP 617
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy TrEMBL
Match: V4U6Q9_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10004237mg PE=4 SV=1) HSP 1 Score: 1000.73 bits (2586), Expect = 0.000e+0 Identity = 507/996 (50.90%), Postives = 674/996 (67.67%), Query Frame = -2
Query: 1173 MTNKRVLSRHLFFKSRRAVTTSAAL-----PLDPSYATISSIPADHFSLCLSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCE-KPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSAN----EELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTF 4130
M ++ S H K+RR TS L PL + S +DH C S ++QLINRGL++SA+++ QR+++NS+ +SDALS DF RG+ D +Y+ L+KKL++ G A Y + + GI PD +I NS++I CKLG +E+A FDRL++ N K A +++R L ++E+FL+AFDYFIK+ N V+L CW YNVLIDGLC KG+L+E +++ ++M ++ +P LH YKSLFY LCK VEAES +MESQGF+VDK MYTSLIN YC R MKMA+R+++RMLK GC PDSYT NTLIHGF KMGLFD GWVL++QM + G QP++VT +MISNYCREG+VD A MLLN+ +S NLAP+ HCYTVLI +LYK NR+ E +E Y++ML + PDH+L F L+K P+G EL A M+L A GCG DPL +SA +L Q+I +L+ +I+KS+ LA VAF + ISALC+ + + A + ++ + G PL+FT N+LIKC Q G E A +++ MQ GI D T LI++ +CK GNL+ A DILDQME RG KP VAIY+ IIG L ++K++ EA MF RML++G+DPDE+ + TMING+ N + IEA +LF +M +++P S+ YTALISGLVKK M D GC YL++ML DG VPN VLYT+LI++FL+ EFEFA RL +LM NQIE DLI YI+LVSG R I+ RK+W + R S+ +E LF L + +L+P+ K+ S K K++ KVK FMPNLYLYN I C RM DAYDHF++M++EG+ PNQVTF IL+ GHI AGEID AIGLFN+MNADGC PD VY L+ GLCQA RL S+ + MHKRG +P K TYE +L+ FC + L++PAF +F+EM+V+ P L CNWLL IL ++ EA IV D + +RG+ P K T+ + K F
Sbjct: 1 MVTRKPGSSHFLSKTRRRAITSCTLEVEREPLANVASASQSTFSDHKMFCFSLADQLINRGLIASAQQVIQRLIANSASLSDALSAADFAAVRGMRFDSGSYSALMKKLIKFGQSQSALLLYQNDFVALGIDPDPAILNSVIIGYCKLGNIEDALRHFDRLISKNIVPIKLACVSILRGLFAEEKFLEAFDYFIKICNAGVDLNCWSYNVLIDGLCYKGFLDEVLEVVNIMRKKKGLVPALHPYKSLFYALCKNRRTVEAESFAREMESQGFYVDKLMYTSLINGYCSNRNMKMAMRLFFRMLKTGCEPDSYTCNTLIHGFFKMGLFDKGWVLYSQMSDWGFQPNMVTDLIMISNYCREGEVDAALMLLNSKVSSNLAPSVHCYTVLIDALYKHNRLMEVDELYKKMLANRVAPDHLLSFILLKNCPEGTELQHALMLLCEFAKIGCGIDPLARSISATLNPTGDLCQEIELLLRKIVKSDPKLANVAFTIYISALCKGGKYEKAYVCLFQLVNFGYRPLVFTCNTLIKCFYQVGFLEGANAIVELMQDTGIVADVETYLIMVEGNCKWGNLDSALDILDQMEVRGPKPSVAIYDAIIGHLCKEKRILEAEDMFKRMLKAGIDPDEVFFTTMINGYLQNRKPIEACQLFEKMKENSVQPGSYPYTALISGLVKKGMVDLGCMYLDRMLADGFVPNVVLYTALINHFLRAGEFEFASRLENLMVTNQIEFDLIAYIALVSGVCRRITGRKKWLDVNRCSDSGKEMLFHKLQQGTLVPRTKSTAFSAVFSNGKKGTVQKIVLKVKDIEFMPNLYLYNDIFLLLCGVGRMDDAYDHFQMMKREGLRPNQVTFCILINGHIAAGEIDQAIGLFNQMNADGCVPDKTVYNTLLKGLCQAGRLSHVFSVFYSMHKRGFVPKKATYEHLLECFCANCLSIPAFNMFKEMIVHDHVPCLSNCNWLLNILFQEKHFHEAQIVLDVMHKRGRLPCKSTRGFWRKHF 996
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy TrEMBL
Match: A0A059CBT8_EUCGR (Uncharacterized protein (Fragment) OS=Eucalyptus grandis GN=EUGRSUZ_D00222 PE=4 SV=1) HSP 1 Score: 985.712 bits (2547), Expect = 0.000e+0 Identity = 489/941 (51.97%), Postives = 675/941 (71.73%), Query Frame = -2
Query: 1239 DPSYATISSIPADHFSLCLSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCEK-PAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELE--QKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISS-RKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVY 4049
+P + ADH SL LS EQLI RG SSAR + +R++ + DA+S V+++ SRGLDLDL++ L++KLVQ G LA ++Y++ ++ RGI+PDS + + +++CLCKLGKL EA FDR + + A +A+ R LC++ L+AFD+F+ +I V++ YN LID LC KGY++EA+++F L E TE PTLH YKSLFYGLCK+G +VEAE L +ME+QGFFVDK MY LI Y K RKMKMA+R+++RMLK+GC D +T+NTLI+GF+K G+FD GW ++ QM++ G+QP+VVT+H+MISNYCREG+VDCA LLN M+ ++ P+ H YT LI SLYK++++TEA+E +RML+ G++PD + F +++M+P+G EL +A+M+L+AI G L + ++ ++ Q I L+E I + +L+LA +AF + ISALCE+ D+A +DK +S+GC +LFTYNSLIKCL KGLFE+AESL + +Q +GI PD T LI+IN +CK GNL+ A+ ++DQ+ +RG+KP VA+Y+CIIGSL K++SEA +F RMLE G+DPDE IY+TMIN ++ +GR++EA LF +MI I+PTS+SYTALI+GLVK++MT+KGC YL+KM+ DG PN VLYTSLI ++L+ EF+FAF LVDLM +NQI+ DL+ YI ++ G R IS + +W R S +AR+ LF LL +SL ++NL+V + PEAMK FAL L +K+K + FMPNL+LYN +ISGFC A+ ++DAY H ELMQ+EG+ PNQVT+TIL+G HI GEID A+GLFNKMNADGC PDG+ Y LV GLC RLL+ LSL++ MHKRGL PSK +Y +L C S L + AFKIFEEM+ ++ P + N L LCV + EA I++
Sbjct: 1 EPPASVAPGNSADHGSLTLSLLEQLIRRGQFSSARSVVRRMIIRAPSAPDAVSAVNYLASRGLDLDLNSCGALLRKLVQCGEYRLAEAWYNENVVDRGIVPDSCMMDPMIVCLCKLGKLGEAKREFDRCVELGALPNVQACDAIFRELCARGMALEAFDFFVGLIGAGVSMRIGCYNQLIDALCLKGYMDEAVKVFCLQQEITELPPTLHQYKSLFYGLCKRGRIVEAEQLSIEMEAQGFFVDKVMYNCLIFGYSKDRKMKMAVRIFFRMLKLGCEVDDHTFNTLIYGFMKWGIFDKGWAMYKQMVDSGIQPTVVTYHIMISNYCREGEVDCALQLLNEMLVCDITPSVHSYTTLIASLYKKDKLTEADELCKRMLDNGVLPDPIFFLVVIRMHPRGCELQLAYMMLQAI-----GNSVSLASNTWDDSMDFGQNIEHLLEGIARKDLNLASIAFAICISALCERGDIDAAILLVDKADSVGCQLMLFTYNSLIKCLCYKGLFEEAESLHDLIQDRGIVPDLETYLIMINGYCKQGNLQSAYRVMDQISERGLKPNVAMYDCIIGSLSSIKRISEAEDLFKRMLEDGMDPDETIYMTMINAYARSGRLLEASELFDKMIDNFIKPTSYSYTALINGLVKRDMTEKGCIYLDKMIGDGYEPNNVLYTSLIGHYLRGGEFKFAFMLVDLMYKNQIKCDLVTYIVVLRGVCRHISGIKHKWGITSRASYKARKMLFDLLQSRSLATIDRNLKVPVNLPEAMKHFALNLFKKIKDSEFMPNLFLYNGLISGFCRANMIEDAYHHVELMQREGLQPNQVTYTILIGEHINRGEIDSAVGLFNKMNADGCLPDGLAYNRLVRGLCSCGRLLDGLSLVYAMHKRGLFPSKLSYNCLLNQLCSSGLNIHAFKIFEEMLTHNIIPSVRCSNLLHSNLCVSGRWHEAQIIH 936 HSP 2 Score: 84.7297 bits (208), Expect = 3.622e-13 Identity = 86/401 (21.45%), Postives = 158/401 (39.40%), Query Frame = -2
Query: 2928 EQLINRGLLSSARKLFQRVVSNSSPV---SDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDN-SCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQ-------------KGYLEEAIQMFDLMPERT--------------------------------ESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERM 3983
+Q+ RGL + ++ ++ + S + S+A + G+D D + Y +I +SG L A + D +I I P S +++ L K E+ I D+++ D + +L+ F AF M + Y V++ G+C+ + + +FDL+ R+ E +P L LY L G C+ + +A M+ +G ++ YT LI + ++ A+ ++ +M GC PD YN L+ G G G L M ++GL PS ++++ +++ C G A + M++ N+ P+ C +L ++L R EA+ + M
Sbjct: 541 DQISERGLKPNV-AMYDCIIGSLSSIKRISEAEDLFKRMLEDGMDPDETIYMTMINAYARSGRLLEASELF-DKMIDNFIKPTSYSYTALINGLVKRDMTEKGCIYLDKMIGDGYEPNNVLYTSLIGHYLRGGEFKFAFMLVDLMYKNQIKCDLVTYIVVLRGVCRHISGIKHKWGITSRASYKARKMLFDLLQSRSLATIDRNLKVPVNLPEAMKHFALNLFKKIKDSEFMPNLFLYNGLISGFCRANMIEDAYHHVELMQREGLQPNQVTYTILIGEHINRGEIDSAVGLFNKMNADGCLPDGLAYNRLVRGLCSCGRLLDGLSLVYAMHKRGLFPSKLSYNCLLNQLCSSGLNIHAFKIFEEMLTHNIIPSVRCSNLLHSNLCVSGRWHEAQIIHHLM 939
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy TrEMBL
Match: U5GGL2_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s21110g PE=4 SV=1) HSP 1 Score: 959.518 bits (2479), Expect = 0.000e+0 Identity = 495/985 (50.25%), Postives = 667/985 (67.72%), Query Frame = -2
Query: 1209 MTNKRVLSRHLFFKSRRA-VTTSAALPLDPSYATISSIPADHFSLCLSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSC--EKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPK--GYELDIAFMVLKAIALNGCGFDPLLLP----VSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSI-SSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGC-TPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYP 4130
M +R L+FK ++ +T++ A+ LDP IP DH SLC S L+ RGLLSSA+++ QR +++S V DA+S V+F ++ G+DL LI+KLV GH A FY D ++ RGI PDS+I NS+VICL KLGKL++A LFDR + C A + +++ Q++F++AFDYF+++ + NV LG W YNVLIDGLCQ+GY+ EAI++ D+M T PTLH+ K+LFYGLCK+GW +EAE +F +ME+QGFFVDK MYTSL+N Y + +KMKMALRVY+RMLK G PD T NTLI+GF KMGLFD WVL+N M + G+QP+ VT+ +MI NYC++GK+DCA LLN+M NL P HCYT ++ +LYK NR E +E+ ERML G+VPDHVLFF LMK PK G+EL + ++L+AIA NGCG D L +++ LEQ+I +L+ EI +S+L+L VA + +SALCE + +SA ++ M + GC+PLLFT+NSLIK L Q GL ED +SL+ MQ GI P+ T LI++NE+CK +L AF IL+QM++ G+KP VAIY+CII L +Q+++SEA +F RMLE+GVDPDE+ Y+TMIN ++ NG+ ++A LF MI AI+P +K+ MT +GC YLEKML +G VPN VLYT LI++FL+ EF++AFRL+DLM R+QIE DL+ +I+ +SG R+I ++KRW+ R S RAR+ LF LLH++ L E VS FMPNLYLYNV +SGFC + ++DAY LMQ+EG+LPN+VTFTIL+G H +AGEID AIGLFN+MNADGC TPD Y L+ LC++ R L+ALSL+H + KRG P++ YE YFC ++++PAF+IFEEMV + P LY N LL ILC ++KL EA D + +RG P
Sbjct: 1 MIKRRPFCHALYFKPKKGPITSTCAVSLDPQ-----PIPNDHTSLCQSLVHDLLRRGLLSSAQQVVQRFIASSPTVHDAISAVEFASASGMDLGPGISGELIRKLVDLGHPLSAREFYHDLVVARGIEPDSNIVNSLVICLAKLGKLDDAVKLFDRHIGSGDCLVSNAACSTILKGFYEQDKFVEAFDYFVRISDANVKLGMWAYNVLIDGLCQQGYVGEAIEVLDIMCRITGLPPTLHMLKTLFYGLCKRGWSIEAEWIFEEMEAQGFFVDKVMYTSLMNAYGRDKKMKMALRVYFRMLKNGYDPDICTCNTLIYGFSKMGLFDKAWVLYNLMNDLGIQPNEVTYSIMIHNYCKKGKLDCAMSLLNSMAPCNLTPCVHCYTPIMVTLYKLNRCLEVDEWCERMLESGIVPDHVLFFVLMKNKPKGLGFELQLCLLMLQAIAKNGCGLDCSSLTNSDKINSTLALEQEIELLLREIARSDLNLGNVAGGIYVSALCEGGKTESALDCLENMVNAGCVPLLFTFNSLIKRLFQDGLSEDVKSLIEIMQNWGISPNLETYLIMVNEYCKQEDLALAFGILEQMDEMGLKPSVAIYDCIIACLSQQRRISEAETLFCRMLENGVDPDEVAYMTMINAYARNGKGVKALHLFEMMIKNAIQP------------MKRKMTIEGCVYLEKMLAEGFVPNIVLYTFLINHFLKMGEFKYAFRLIDLMFRSQIEADLVLHIAWISGVCRNIFGTKKRWYMTNRMSTRARKLLFNLLHQKVSLTGEDVFSVS--------------------AWFMPNLYLYNVTVSGFCWVNLIEDAYHQLRLMQEEGLLPNEVTFTILIGAHGRAGEIDRAIGLFNRMNADGCSTPDRCTYNTLLKSLCRSGRELDALSLVHTISKRGFFPNRLAYEKSHHYFCAGHMSIPAFRIFEEMVACNLVPGLYRRNLLLYILCEEKKLHEAYRASDVMFERGFLP 948 HSP 2 Score: 135.576 bits (340), Expect = 9.490e-29 Identity = 126/517 (24.37%), Postives = 219/517 (42.36%), Query Frame = -2
Query: 2022 NVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVILIEEILKSN---LHLAKVAFNVL---ISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMK-PGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFE 3551
++NLG + + LC+ G E A+ + M +P L + SL L + G + +SL M++ G + Y ++N YCK + +A + +M +MG +P Y+ +I + LF +M+E G+ P V + MI+ Y R GK A L MI + P K E + E+ML G VP+ VL+ L+ + K E AF ++ + + D L+L ++ + + I + +N K+ FN+L +S E SA + MP L+ YN + L EDA L MQ +G+ P++ T I+I H + G ++ A + ++M G P YN ++ SL R + +A ++ + + G P+ + Y + F I A R+F EM+ + P + L+ L ++ + + M + G +P+ + + L FL + +F
Sbjct: 475 DLNLGNVAGGIYVSALCEGGKTESALDCLENMVN-AGCVPLLFTFNSLIKRLFQDGLSEDVKSLIEIMQNWGISPNLETYLIMVNEYCKQEDLALAFGILEQMDEMGLKPSVAIYDCIIACLSQQRRISEAETLFCRMLENGVDPDEVAYMTMINAYARNGKGVKALHLFEMMIKNAIQP------------MKRKMTIEGCVYLEKMLAEGFVPNIVLYTFLINHFLKMGEFKYAFRLIDLMFRSQIEAD-LVLHIAWISGVCRNIFGTKKRWYMTNRMSTRARKLLFNLLHQKVSLTGEDVFSVSAWF----------MPNLYLYNVTVSGFCWVNLIEDAYHQLRLMQEEGLLPNEVTFTILIGAHGRAGEIDRAIGLFNRMNADGCSTPDRCTYNTLLKSLCRSGRELDALSLVHTISKRGFFPNRLAYEKSHHYFCAGHMSIPAFRIFEEMVACNLVPGLYRRNLLLYILCEEKKLHEAYRASDVMFERGFLPDESVMSPL---FLSRLDFH 964
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy TrEMBL
Match: K4BIJ2_SOLLC (Uncharacterized protein OS=Solanum lycopersicum GN=Solyc03g093150.1 PE=4 SV=1) HSP 1 Score: 943.725 bits (2438), Expect = 0.000e+0 Identity = 483/966 (50.00%), Postives = 668/966 (69.15%), Query Frame = -2
Query: 1221 KSRRAVTTSAALPLDPSYAT--ISSIPADHFSLCLSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDN--SCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVI----LIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISS-RKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQR 4091
+++R +TTS PL PS A + + P +H +LC S + LI RGL SA+K+ +R++ +SS V +A+S V+F SRG++ D+++YA LI++LV SG A + Y D I+ RGI P+ S+ NS+ IC C LGKLEEA +LFD+L+ C N L++ C Q+R LD FD F++ IN V L YN L+D LC +GYL+EA+ +FD M +R PT+HL+K L L K+G V EA+ L MES GF +DK MYT+LIN Y K +KMK A+ ++ RM K+GC PD YTYNTLI+GF+ +G+FD GW+L QM+E GL+P V++ +MI+ YC+E KVDCA LL+++ N+ P+ H YT LI++LYKENR+ E ++ Y +ML GLVPDHVLFF L+ +P+G E+ +A L+AIA NGCG DP +P + ++ I++ L+ EI NL LA VAFN+ + ALC + DSA MDKM SL P L YNS+IKCL QKGL EDA+ L+ MQ QG P+QAT LI++NE+CK G+++ A ++LDQME+ G+KP VAIY+ +IG L R+K++ EA +F RMLE+G+ PD+ +++TMIN S NGR I+A LF M+ ++P+ ++YTALI+GLVKKNM +KGC YL++M+++G +PN VLYTSLI FL+KREFEFA +LVDLM+R+++ERDL+ YI+LVSG R+I S ++ +R E ++E LF+LLH+ ++LPKEK L++S +S E +K AL+LI KVK T MPNLYLYN IISGFC A+ M+DAY H MQ EG+LPNQVTFTIL+ GH ++GEI+ A+ LFN+MNA GC PD IVY L+ GLC+ RL++ALSL + M K+GL PSK +YE +L C S V A KI +M+ P + L+CIL + K EA +YD ++++
Sbjct: 18 RTKRPLTTS---PL-PSEAISCVHTSPLNHKTLCFSLAANLIVRGLFDSAQKVIRRIIKHSSSVPEAISAVEFSISRGVEPDVTSYAFLIRQLVTSGETLKAEALYVDCILNRGIEPNHSLLNSMAICYCNLGKLEEAKLLFDKLVDMKLMPCSSTC-NELIKGFCGQDRILDGFDVFVEAINSEVLLAFSCYNKLVDILCFQGYLDEALYVFDEMCDRGVP-PTVHLFKRLILSLSKRGRVEEAQLLSMDMESYGFVLDKVMYTTLINGYSKIQKMKTAMMLFLRMRKLGCEPDKYTYNTLINGFINLGMFDKGWMLNQQMVEFGLEPDAVSYQIMIAKYCKEHKVDCALTLLDDINQWNVPPSVHSYTALISALYKENRLAEVDDLYRKMLYTGLVPDHVLFFTLISNHPRGSEISLACTFLRAIAKNGCGIDPSFIPSPTSRKVTTDIMLDIDCLLGEIAARNLPLACVAFNIYMIALCLGGELDSAQLCMDKMSSLSLQPSLSAYNSMIKCLYQKGLHEDAKLLVEVMQDQGQVPNQATFLIMVNEYCKQGDIQSALEVLDQMEESGLKPSVAIYDSVIGCLGRKKRIDEALGVFRRMLETGIYPDKTLFVTMINALSRNGRAIQAHELFVTMLEDGVQPSHNAYTALINGLVKKNMIEKGCVYLKQMIEEGFMPNTVLYTSLIKQFLRKREFEFALKLVDLMERSEVERDLVTYITLVSGVSRNIRSVNEKGLVPQRRYEESKEMLFRLLHQSAMLPKEKCLKISVNSQEQIKFLALRLINKVKATPLMPNLYLYNGIISGFCWAESMKDAYKHLHTMQNEGILPNQVTFTILIDGHFRSGEINCAVSLFNRMNAQGCPPDNIVYNTLIRGLCKHGRLMDALSLSYTMLKKGLAPSKASYESLLSSLCASNWRVHALKICHDMLANKYVPCGHNLKLLICILGEENKWHEARFMYDLLLKK 977 HSP 2 Score: 168.318 bits (425), Expect = 6.606e-39 Identity = 165/643 (25.66%), Postives = 272/643 (42.30%), Query Frame = -2
Query: 1173 ISKNLAPNAHCYTVLITSLYKENRITEAEEFY-ERMLNGGLVPDHVLFFKLMKMY---PKGYELDIAFMVLKAIALNGCGF--DPLLLPVSANEELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPK--EKNLRVSDSSPEAMKCFALKLI---------------QKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTF 3032
IS+ + P+ Y LI L +AE Y + +LN G+ P+H L + Y K E + F L + L C + L+ + + + +E I S + LA +N L+ LC Q D A Y D+M G P + + LI LS++G E+A+ L M++ G D+ +IN + K ++ A + +M G +P YN +I + + +M+E G++PD + Y MI + +V A L ++ + P+ HSYTALIS L K+N + KML GLVP+ VL+ +LISN + E A + + +N D F S +SRK + +L LL + +NL P A F + +I K+ P+L YN +I + +DA E+MQ +G +PNQ TF I++ + K G+I A+ + ++M G P +Y ++ L + R+ EAL + M + G+ P K + ++ + A+ A ++F M+ P L+ L + + + C+ +I+ G P + IK F
Sbjct: 88 ISRGVEPDVTSYAFLIRQLVTSGETLKAEALYVDCILNRGIEPNHSLLNSMAICYCNLGKLEEAKLLFDKLVDMKLMPCSSTCNELIKGFCGQDRILDGFDVFVEAI-NSEVLLAFSCYNKLVDILCFQGYLDEALYVFDEMCDRGVPPTVHLFKRLILSLSKRGRVEEAQLLSMDMESYGFVLDKVMYTTLINGYSKIQKMKTAMMLFLRMRKLGCEPDKYTYNTLINGFINLGMFDKGWMLNQQMVEFGLEPDAVSYQIMIAKYCKEHKVDCALTLLDDINQWNVPPSVHSYTALISALYKENRLAEVDDLYRKMLYTGLVPDHVLFFTLISNHPRGSEISLACTFLRAIAKNGCGID--------PSFIPSPTSRKVTTDI-------------MLDIDCLLGEIAARNL------PLACVAFNIYMIALCLGGELDSAQLCMDKMSSLSLQPSLSAYNSMIKCLYQKGLHEDAKLLVEVMQDQGQVPNQATFLIMVNEYCKQGDIQSALEVLDQMEESGLKPSVAIYDSVIGCLGRKKRIDEALGVFRRMLETGIYPDKTLFVTMINALSRNGRAIQAHELFVTMLEDGVQPSHNAYTALINGLVKKNMIEKGCVYLKQMIEEGFMPNTVLYTSLIKQF 702
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy TrEMBL
Match: M1B6U4_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG402014825 PE=4 SV=1) HSP 1 Score: 941.799 bits (2433), Expect = 0.000e+0 Identity = 482/966 (49.90%), Postives = 667/966 (69.05%), Query Frame = -2
Query: 1221 KSRRAVTTSAALPLDPSYAT--ISSIPADHFSLCLSFSEQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDN--SCEKPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSANEELEQKIVI----LIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISS-RKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQR 4091
+++R +TTS PL PS A + + P +H +LC S ++ LI RGL SA K+ +R++ +SS VS+A+S V+F SRG++ D ++YA L ++LV S A + Y D I+ RGI P+ S+ NS+ IC C LGKLEEA +LFD+L+ C N L++ C Q+R LD FD F++ IN V L YN L+DGLC +GYL+EA+ +FD M +R PT+HL+K+L L K+G V EA+ L MES GF +DK MYT+LIN Y K +KMK A+ ++ RM K+GC PD YTYNTLI+GF+ +G+FD GW+L QM+E GL+P V++ +MI+ YC++ KVDCA LLN++ N+ P+ H YT LI++LYKENR+ E ++ Y +ML GLVPDHVLFF L+ +P+G E+ +A L+AIA NGCG D +P + ++ I++ L+ EI+ NL LA VAFN+ + ALC + DSA MDKM SL P L YNS+IKCL QKGL EDA+ L+ MQ QG P+QAT LI++NE+CK G+++ A ++LDQME+ G+KP VAIY+ +IG L R+K++ EA +F RMLE+G+ PD+I+++TMIN S NG+ I+A LF M+ ++P+ ++YTALI+GLVKKNM +KGC YL++M+++G +PN VLYTSLI FL+KR+FEFA +LVDLM+R++IERDL+ YI+LVSG R+I S + +R E ++E LF+LLH+ ++LPKEK L++S SS E +K AL+LI KVK T MPNLYLYN IISGFC A+ M+DAY H MQ EG+ PNQVTFTIL+ GH ++GEI+ A+ LFN+MNA GC PD IVY L+ GLC+ RL++ALSL + M K+GL PSK +YE +L C S V A KI +M+ P + L+CIL + K EA +YD ++++
Sbjct: 18 RTKRPLTTS---PL-PSEAISCVHTSPVNHKTLCFSLADNLIVRGLFDSAEKVIRRIIKHSSSVSEAISAVEFSISRGVEPDATSYAFLFRQLVTSRETLKAEALYVDCILNRGIEPNHSVLNSMAICYCNLGKLEEAKLLFDKLVDKKLLPCSSTC-NELIKGFCGQDRILDGFDVFVEAINSEVLLAFSCYNKLVDGLCFRGYLDEALYVFDEMCDRGVP-PTVHLFKTLILSLSKRGRVEEAQLLSMDMESYGFVLDKVMYTTLINGYSKIQKMKTAMMLFLRMRKLGCEPDKYTYNTLINGFINLGMFDKGWMLNQQMVEFGLEPDAVSYQIMIAKYCKDHKVDCALTLLNDINQCNVPPSVHSYTALISALYKENRLAEVDDLYRKMLYTGLVPDHVLFFTLISNHPRGSEISLACTFLRAIAKNGCGIDLSYIPSPTSRKVTTDIMLDIDRLLGEIVARNLPLASVAFNIYMIALCLGGELDSAQLCMDKMSSLSLQPSLSAYNSMIKCLYQKGLHEDAKFLVEVMQDQGQVPNQATFLIMVNEYCKQGDIQSALEVLDQMEESGLKPSVAIYDSVIGCLGREKRIDEALGVFRRMLEAGIYPDKILFVTMINALSRNGQAIQAHELFITMLEDGVQPSHYAYTALINGLVKKNMIEKGCVYLKQMIEEGFMPNTVLYTSLIKQFLRKRQFEFALKLVDLMERSEIERDLVTYITLVSGVSRNIRSVDGKGLVPQRRYEESKEMLFRLLHQSAMLPKEKCLKISVSSQEQIKFLALRLINKVKATPLMPNLYLYNGIISGFCWAESMEDAYKHLHTMQNEGIQPNQVTFTILIDGHFRSGEINCAVSLFNRMNAQGCPPDNIVYNTLIRGLCRHGRLVDALSLSYTMLKKGLAPSKASYESLLSSLCASNWRVHALKICHDMLANKYVPCGHNLKLLICILGEENKWHEARFMYDLLLKK 977 HSP 2 Score: 175.252 bits (443), Expect = 4.384e-41 Identity = 165/638 (25.86%), Postives = 283/638 (44.36%), Query Frame = -2
Query: 1173 ISKNLAPNAHCYTVLITSLYKENRITEAEEFY-ERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSA--NEEL-----EQKIV----ILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEK------NLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTF 3032
IS+ + P+A Y L L +AE Y + +LN G+ P+H + + Y +L+ A ++ + D LLP S+ NE + + +I+ + +E I S + LA +N L+ LC + D A Y D+M G P + + +LI LS++G E+A+ L M++ G D+ +IN + K ++ A + +M G +P YN +I + + +M+E G++PD + Y MI + + +V A L +++ + P+ HSYTALIS L K+N + KML GLVP+ VL+ +LISN + E A + + +N DL + S +SRK + +R L +++ R L L + A C + K+ P+L YN +I + +DA E+MQ +G +PNQ TF I++ + K G+I A+ + ++M G P +Y ++ L + R+ EAL + M + G+ P K + ++ + A+ A ++F M+ P Y L+ L + + + C+ +I+ G P + IK F
Sbjct: 88 ISRGVEPDATSYAFLFRQLVTSRETLKAEALYVDCILNRGIEPNHSVLNSMAICYCNLGKLEEAKLLFDKLV------DKKLLPCSSTCNELIKGFCGQDRILDGFDVFVEAI-NSEVLLAFSCYNKLVDGLCFRGYLDEALYVFDEMCDRGVPPTVHLFKTLILSLSKRGRVEEAQLLSMDMESYGFVLDKVMYTTLINGYSKIQKMKTAMMLFLRMRKLGCEPDKYTYNTLINGFINLGMFDKGWMLNQQMVEFGLEPDAVSYQIMIAKYCKDHKVDCALTLLNDINQCNVPPSVHSYTALISALYKENRLAEVDDLYRKMLYTGLVPDHVLFFTLISNHPRGSEISLACTFLRAIAKNGCGIDL--------SYIPSPTSRKVTTDIMLDIDRL---LGEIVARNLPLASVAFNIYMIALCLGGELDSAQLC-----MDKMSSLSLQPSLSAYNSMIKCLYQKGLHEDAKFLVEVMQDQGQVPNQATFLIMVNEYCKQGDIQSALEVLDQMEESGLKPSVAIYDSVIGCLGREKRIDEALGVFRRMLEAGIYPDKILFVTMINALSRNGQAIQAHELFITMLEDGVQPSHYAYTALINGLVKKNMIEKGCVYLKQMIEEGFMPNTVLYTSLIKQF 702
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy TrEMBL
Match: A0A067FPQ9_CITSI (Uncharacterized protein (Fragment) OS=Citrus sinensis GN=CISIN_1g043758mg PE=4 SV=1) HSP 1 Score: 926.391 bits (2393), Expect = 0.000e+0 Identity = 474/942 (50.32%), Postives = 630/942 (66.88%), Query Frame = -2
Query: 1173 EQLINRGLLSSARKLFQRVVSNSSPVSDALSTVDFVTSRGLDLDLSTYAVLIKKLVQSGHLPLAYSFYSDYIIGRGIIPDSSIANSIVICLCKLGKLEEATILFDRLLTDNSCE-KPAFNALVRLLCSQERFLDAFDYFIKMININVNLGCWYYNVLIDGLCQKGYLEEAIQMFDLMPERTESLPTLHLYKSLFYGLCKQGWVVEAESLFGKMESQGFFVDKTMYTSLINVYCKGRKMKMALRVYYRMLKMGCRPDSYTYNTLIHGFVKMGLFDYGWVLFNQMMEQGLQPSVVTFHVMISNYCREGKVDCASMLLNNMISKNLAPNAHCYTVLITSLYKENRITEAEEFYERMLNGGLVPDHVLFFKLMKMYPKGYELDIAFMVLKAIALNGCGFDPLLLPVSAN----EELEQKIVILIEEILKSNLHLAKVAFNVLISALCEQAQQDSASYFMDKMESLGCMPLLFTYNSLIKCLSQKGLFEDAESLLNRMQAQGIFPDQATCLIIINEHCKHGNLEPAFDILDQMEDRGMKPGVAIYNCIIGSLFRQKKVSEATAMFVRMLESGVDPDEIIYLTMINGFSNNGRVIEADRLFHEMIGAAIRPTSHSYTALISGLVKKNMTDKGCTYLEKMLDDGLVPNAVLYTSLISNFLQKREFEFAFRLVDLMDRNQIERDLIFYISLVSGFYRSISSRKRWFAMRRGSERAREKLFQLLHRQSLLPKEKNLRVSDSSPEAMKCFALKLIQKVKQTRFMPNLYLYNVIISGFCEADRMQDAYDHFELMQKEGVLPNQVTFTILMGGHIKAGEIDHAIGLFNKMNADGCTPDGIVYKILVNGLCQASRLLEALSLLHVMHKRGLIPSKGTYEIILQYFCDSYLAVPAFKIFEEMVVYSPGPQLYYCNWLLCILCVQQKLREACIVYDSIIQRGKYPYKPTKSLFIKTF 3983
+QLINRGL++SA+++ QR+++NS+ +SDALS DF RG+ D +Y+ L+KKL++ G A Y + + LG +E+A FDRL++ N K A +++R L ++E+FL+AFDYFIK+ N V+L CW YNVLIDGLC KG+L+E +++ ++M ++ +P LH YKSLFY LCK VEAES +MESQGF+VDK MYTSLIN YC R MKMA+R+++RMLK GC PDSYT NTLIHGF KMGLFD GWVL++QM + G QP++VT +MISNYCREG+VD A MLLN+ +S NLAP+ HCYTVLI +LYK NR+ E +E Y++ML + PDH+L F L+K P+G EL A M+L A GCG DPL +SA +L Q+I +L+ +I+KS+ LA VAF + ISALC+ + + A + ++ + G PL+FT N+LIKC Q G E A +++ MQ +CK GNL+ A DILDQME RG KP VAIY+ IIG L ++K++ EA MF RML++G+DPDE+ + TMING+ N + IEA +LF +M +++P S+ YTALISGLVKK M D GC YL++ML DG VPN VLYT+LI++FL+ EFEFA RL +LM NQIE DLI YI+LVSG R I+ RK+W + R S+ +E LF L + +L+ + K+ S K K++ KVK FMPNLYLYN I C RM DAYDHF++M++EG+ PNQVTF IL+ GHI AGEID AIGLFN+MNADGC PD VY L+ GLCQA RL S+ + MHKRG +P K TYE +L+ FC + L++PAF +F+EM+V+ P L CNWLL ILC ++ EA IV D + +RG+ P T+ + K F
Sbjct: 1 DQLINRGLIASAQQVIQRLIANSASLSDALSAADFAAVRGMRFDSGSYSALMKKLIKFGQSQSALLLYQNDFVA-------------------LGNIEDALRHFDRLISKNIVPIKLACVSILRGLFAEEKFLEAFDYFIKICNAGVDLNCWSYNVLIDGLCYKGFLDEVLEVVNIMRKKKGLVPALHPYKSLFYALCKNIRTVEAESFAREMESQGFYVDKLMYTSLINGYCSNRNMKMAMRLFFRMLKTGCEPDSYTCNTLIHGFFKMGLFDKGWVLYSQMSDWGFQPNMVTDLIMISNYCREGEVDAALMLLNSKVSSNLAPSVHCYTVLIDALYKHNRLMEVDELYKKMLANRVAPDHLLSFILLKNCPEGTELQHALMLLCEFAKIGCGIDPLARSISATLNPTGDLCQEIELLLRKIVKSDPKLANVAFTIYISALCKGGKYEKAYVCLFQLVNFGYRPLVFTCNTLIKCFYQVGFLEGANAIVELMQDT-------------EGNCKWGNLDSALDILDQMEVRGPKPSVAIYDAIIGHLCKEKRILEAEDMFKRMLKAGIDPDEVFFTTMINGYLQNRKPIEACQLFEKMKENSVQPGSYPYTALISGLVKKGMVDLGCMYLDRMLADGFVPNVVLYTALINHFLRAGEFEFASRLENLMVTNQIEFDLIAYIALVSGVCRRITGRKKWLDVNRCSDSGKEMLFHKLQQGTLVTRTKSTAFSAVFSNGKKGTVQKIVLKVKDIEFMPNLYLYNDIFLLLCGVGRMDDAYDHFQMMKREGLRPNQVTFCILINGHIAAGEIDQAIGLFNQMNADGCVPDKTVYNTLLKGLCQAGRLSHVFSVFYSMHKRGFVPKKATYEHLLECFCANCLSIPAFNMFKEMIVHDHVPCLSNCNWLLNILCQEKHFHEAQIVLDVMHKRGRLPCTSTRGFWRKHF 910 The following BLAST results are available for this feature:
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy Swiss-Prot
Analysis Date: 2016-11-01 (Homology Analysis for Gossypium barbadense CottonGen RefTrans V1 vs Swissprot) Total hits: 25
Pagesback to top
BLAST of g.barbadense_cottongen_reftransV1_0094142 vs. ExPASy TrEMBL
Analysis Date: 2016-11-02 (Homology Analysis for Gossypium barbadense CottonGen RefTrans V1 vs TrEMBL) Total hits: 25
Pagesback to topInterPro
Analysis Name: InterProScan analysis for Gossypium barbadense CottonGen RefTrans V1
Date Performed: 2016-11-02
Sequences
The
following sequences are available for this feature:
contig sequence >g.barbadense_cottongen_reftransV1_0094142 ID=g.barbadense_cottongen_reftransV1_0094142; Name=g.barbadense_cottongen_reftransV1_0094142; organism=Gossypium barbadense; type=contig; length=4335bpback to top |