g.barbadense_cottongen_reftransV1_0092647, g.barbadense_cottongen_reftransV1_0092647 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy Swiss-Prot
Match: ASHR1_ARATH (Histone-lysine N-methyltransferase ASHR1 OS=Arabidopsis thaliana GN=ASHR1 PE=2 SV=2)

HSP 1 Score: 294.278 bits (752), Expect = 1.610e-86
Identity = 134/185 (72.43%), Postives = 159/185 (85.95%), Query Frame = 3

Query:  156 MEELQASLQPRGLTLSTFPDKGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFSKSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDIDEKQLLLYAQMANLVNLILQLPNIDIKEIAENFSK 710
            M +LQ  LQ R L +S  P KGRSL  ARDF PGEVI+SQ+PY+CVPNN+ +ESRCDGCF  +NLKKCSAC VVWYCGSSCQK EWKLHR EC+ L +L+KE+RK VTPTIR+MV+LY++R LQNE V+P+T  DNY+LVEALVSHMS+IDEKQ+LLYAQMANLVNLILQ P++D++EIAENFSK
Sbjct:    1 MADLQRFLQDRCLGVSNLPQKGRSLFTARDFRPGEVILSQKPYICVPNNTSSESRCDGCFKTNNLKKCSACQVVWYCGSSCQKSEWKLHRDECKALTRLEKEKRKFVTPTIRLMVRLYIKRNLQNEKVLPITTTDNYSLVEALVSHMSEIDEKQMLLYAQMANLVNLILQFPSVDLREIAENFSK 185          

HSP 2 Score: 233.802 bits (595), Expect = 3.502e-65
Identity = 104/156 (66.67%), Postives = 135/156 (86.54%), Query Frame = 3

Query: 1176 QFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCNKLGQYGDIQESAILEGYRCRDNGCSGFLLRESDEKGFVCQQCGLIRNKEEIRKIASDIKALSDKA 1643
            +F+CNAH+ICDSELR  G GL+P++SIINHSC PNAVLVFE ++AVVRA+ +I KDSE++ISY+ETA ST+TRQK+LKEQYLF C C RC+  G+  DI+ESAILEGYRC +  C+GFLLR+ +EKGFVCQ+C L+R+KEE++K+ASD+K +S+KA
Sbjct:  185 KFSCNAHSICDSELRPQGIGLFPLVSIINHSCSPNAVLVFEEQMAVVRAMDNISKDSEITISYIETAGSTLTRQKSLKEQYLFHCQCARCSNFGKPHDIEESAILEGYRCANEKCTGFLLRDPEEKGFVCQKCLLLRSKEEVKKLASDLKTVSEKA 340          

HSP 3 Score: 73.9442 bits (180), Expect = 3.373e-12
Identity = 28/41 (68.29%), Postives = 34/41 (82.93%), Query Frame = 1

Query: 2269 EEWKEALAICRLTIPVYERVYPGFHPLLGLQYYSCGKLEWL 2391
            E W+EAL  CRL +PVY+RVYP  HPL+GLQ+Y+ GKLEWL
Sbjct:  389 EIWREALNYCRLIVPVYQRVYPATHPLIGLQFYTQGKLEWL 429          

HSP 4 Score: 72.4034 bits (176), Expect = 1.221e-11
Identity = 34/47 (72.34%), Postives = 38/47 (80.85%), Query Frame = 1

Query: 2920 LLGETDDAIKSFTKAVDILRITHGTSTPFMKELLMKLEEARVEASFK 3060
            LLGET +A+ S  KA DILRI+HG STPFMKEL  KLEEAR EAS+K
Sbjct:  429 LLGETKEAVSSLIKAFDILRISHGISTPFMKELSAKLEEARAEASYK 475          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy Swiss-Prot
Match: SMYD3_MOUSE (Histone-lysine N-methyltransferase SMYD3 OS=Mus musculus GN=Smyd3 PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 5.209e-18
Identity = 39/96 (40.62%), Postives = 64/96 (66.67%), Query Frame = 3

Query: 1185 CNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCNKLGQYGDI 1472
            CN+ TIC++E++ +G GLYP +S++NHSC PN  +VF G   ++RAV+ I    E++I Y++   ++  R+K L++QY F C C+RC    +  D+
Sbjct:  180 CNSFTICNAEMQEVGVGLYPSMSLLNHSCDPNCSIVFNGPHLLLRAVREIEAGEELTICYLDMLMTSEERRKQLRDQYCFECDCIRCQTQDKDADM 275          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy Swiss-Prot
Match: SMYD3_HUMAN (Histone-lysine N-methyltransferase SMYD3 OS=Homo sapiens GN=SMYD3 PE=1 SV=4)

HSP 1 Score: 90.5077 bits (223), Expect = 1.378e-17
Identity = 40/96 (41.67%), Postives = 63/96 (65.62%), Query Frame = 3

Query: 1185 CNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCNKLGQYGDI 1472
            CN+ TIC++E++ +G GLYP IS++NHSC PN  +VF G   ++RAV+ I    E++I Y++   ++  R+K L++QY F C C RC    +  D+
Sbjct:  180 CNSFTICNAEMQEVGVGLYPSISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYLDMLMTSEERRKQLRDQYCFECDCFRCQTQDKDADM 275          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy Swiss-Prot
Match: SMY2B_XENLA (N-lysine methyltransferase SMYD2-B OS=Xenopus laevis GN=smyd2-b PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.607e-16
Identity = 60/197 (30.46%), Postives = 95/197 (48.22%), Query Frame = 3

Query:  180 QPRGLTLSTFPDKGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFS-KSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDID-EKQLLLYAQMANLVNLILQ-LPNIDIKEIAENFSKVHIERF*ASNSLVYHLS 761
            QP GL     P KGR L A R F  GE++ S   Y  V  ++   + CD CF+ K  L KC  C   +YC   CQK +W +H+LEC  +    +    S   T+R+  ++  ++K Q E     TA + +  V+   SH+S +D EK+ L+   ++ L     + + N D   +   F++V+   F   +  + HL 
Sbjct:    3 QPEGLERFDSPGKGRGLKATRSFALGELLFSCPAYTYVLTDNERGNHCDFCFTRKEGLSKCGKCKQAFYCNVDCQKGDWPMHKLECSSMCSSGQNWCPS--ETVRLTARILAKQKTQTER----TASERFMSVKEFESHLSKLDNEKKELIENDISALHRFYSKNVHNCDNAALEFLFAQVNCNGFTIEDEELSHLG 193          

HSP 2 Score: 86.2705 bits (212), Expect = 3.190e-16
Identity = 43/119 (36.13%), Postives = 63/119 (52.94%), Query Frame = 3

Query: 1092 FYCNKVRNLNKFCLSWMNICSYLLL*KWQFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCN 1448
            FY   V N +   L ++           Q  CN  TI D EL  LG+ ++P ++++NHSC PN ++ ++G +A VRAVQ I    EV  SY++    T  R   LK+ Y F+C C  C+
Sbjct:  157 FYSKNVHNCDNAALEFLFA---------QVNCNGFTIEDEELSHLGSAIFPDVALMNHSCCPNVIVTYKGTVAEVRAVQEIHAGEEVFTSYIDLLYPTEDRNDRLKDSYFFSCDCRECS 266          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy Swiss-Prot
Match: SMYD2_CHICK (N-lysine methyltransferase SMYD2 OS=Gallus gallus GN=SMYD2 PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 3.148e-16
Identity = 38/91 (41.76%), Postives = 54/91 (59.34%), Query Frame = 3

Query: 1176 QFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCN 1448
            Q  CN  TI D EL  LG+ ++P ++++NHSC PN ++ ++G LA VRAV+ I    EV  SY++    T  R   L++ Y FTC C  C 
Sbjct:  181 QVNCNGFTIEDEELSHLGSAIFPDVALMNHSCCPNVIVTYKGTLAEVRAVKEIEPGEEVFTSYIDLLYPTEDRNDRLRDSYFFTCDCRECT 271          

HSP 2 Score: 85.5001 bits (210), Expect = 6.631e-16
Identity = 54/165 (32.73%), Postives = 80/165 (48.48%), Query Frame = 3

Query:  168 QASLQPRGLTLSTFPDKGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFS-KSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDID-EKQLLLYAQMANLVNL 656
            +A  QP GL     P KGR L A R +  GE++ S   Y  V   S   S CDGCF+ K  L KC  C   +YC   CQK +W +H+LEC  +    +    S   T+R+  ++  ++K+  E     T  +    V+   SH+  +D EK+ L+   +A L + 
Sbjct:    4 EAVPQPGGLERFASPGKGRGLRALRRYAVGELLFSCPAYTAVLTVSERGSHCDGCFARKEGLSKCGRCKQAFYCNVECQKEDWPMHKLECAAMCAFGQNWNPS--ETVRLTARILAKQKIHPER----TQSEKLLAVKEFESHLDKLDNEKRELIQNDIAALHHF 162          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy Swiss-Prot
Match: SMYD2_HUMAN (N-lysine methyltransferase SMYD2 OS=Homo sapiens GN=SMYD2 PE=1 SV=2)

HSP 1 Score: 85.8853 bits (211), Expect = 4.323e-16
Identity = 39/90 (43.33%), Postives = 54/90 (60.00%), Query Frame = 3

Query: 1176 QFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRC 1445
            Q  CN  TI D EL  LG+ ++P ++++NHSC PN ++ ++G LA VRAVQ I    EV  SY++    T  R   L++ Y FTC C  C
Sbjct:  178 QVNCNGFTIEDEELSHLGSAIFPDVALMNHSCCPNVIVTYKGTLAEVRAVQEIKPGEEVFTSYIDLLYPTEDRNDRLRDSYFFTCECQEC 267          

HSP 2 Score: 68.1662 bits (165), Expect = 2.052e-10
Identity = 49/169 (28.99%), Postives = 80/169 (47.34%), Query Frame = 3

Query:  189 GLTLSTFPDKGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFS-KSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDID-EKQLLLYAQMANLVNLI---LQLPNID 680
            GL     P KGR L A + F  G+++ S   Y  V   +   + C+ CF+ K  L KC  C   +YC   CQK +W +H+LEC  +    +    S   T+R+  ++  ++K+  E     T  +    V+   SH+  +D EK+ L+ + +A L +     L  P+ D
Sbjct:    8 GLERFCSPGKGRGLRALQPFQVGDLLFSCPAYAYVLTVNERGNHCEYCFTRKEGLSKCGRCKQAFYCNVECQKEDWPMHKLECSPMVVFGENWNPS--ETVRLTARILAKQKIHPER----TPSEKLLAVKEFESHLDKLDNEKKDLIQSDIAALHHFYSKHLGFPDND 170          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy Swiss-Prot
Match: SMY2A_XENLA (N-lysine methyltransferase SMYD2-A OS=Xenopus laevis GN=smyd2-a PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 5.141e-16
Identity = 59/197 (29.95%), Postives = 94/197 (47.72%), Query Frame = 3

Query:  180 QPRGLTLSTFPDKGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFS-KSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDID-EKQLLLYAQMANLVNLILQ-LPNIDIKEIAENFSKVHIERF*ASNSLVYHLS 761
            QP GL     P KGR L A R F  GE++ +   Y  V  ++   + CD CF+ K  L KC  C   +YC   CQK +W +H+LEC  +    +    S   T+R+  ++  ++K Q E     T  + +  V+   SH+S +D EK+ L+ + +A L     + L   D   +   F++V+   F   +  + HL 
Sbjct:    3 QPEGLERFDSPGKGRGLKATRSFALGELLFTCPAYTYVLTDTERGNHCDFCFARKEGLSKCGKCKQAFYCNVDCQKGDWPMHKLECSAMCSYGQNWCPS--ETVRLTARILAKQKTQTER----TPSETFLSVKEFESHLSKLDNEKKELIESDIAALHRFYSKNLHYTDNAALVFLFAQVNCNGFTIEDEELSHLG 193          

HSP 2 Score: 85.5001 bits (210), Expect = 5.991e-16
Identity = 38/91 (41.76%), Postives = 54/91 (59.34%), Query Frame = 3

Query: 1176 QFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCN 1448
            Q  CN  TI D EL  LG+ ++P ++++NHSC PN ++ F+G +A +RAVQ I    EV  SY++    T  R   L + Y FTC C  C+
Sbjct:  176 QVNCNGFTIEDEELSHLGSAIFPDVALMNHSCCPNIIVTFKGTVAEIRAVQEIHAGDEVFTSYIDLLYPTEDRNDRLMDSYFFTCDCRECS 266          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy Swiss-Prot
Match: SMYD2_BOVIN (N-lysine methyltransferase SMYD2 OS=Bos taurus GN=SMYD2 PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 5.661e-16
Identity = 39/90 (43.33%), Postives = 54/90 (60.00%), Query Frame = 3

Query: 1176 QFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRC 1445
            Q  CN  TI D EL  LG+ ++P ++++NHSC PN ++ ++G LA VRAVQ I    EV  SY++    T  R   L++ Y FTC C  C
Sbjct:  178 QVNCNGFTIEDEELSHLGSAIFPDVALMNHSCCPNVIVTYKGTLAEVRAVQEIHPGEEVFTSYIDLLYPTEDRNDRLRDSYFFTCECQEC 267          

HSP 2 Score: 69.707 bits (169), Expect = 6.261e-11
Identity = 49/169 (28.99%), Postives = 81/169 (47.93%), Query Frame = 3

Query:  189 GLTLSTFPDKGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFS-KSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDID-EKQLLLYAQMANLVNLI---LQLPNID 680
            GL     P KGR L A + F  G+++ S   Y  V   S   + C+ CF+ K  L KC  C   +YC   CQ+ +W +H+LEC  +    +    S   T+R+  ++  ++K+  E     T  +    V+   SH+  +D EK+ L+ + +A L +     L+ P+ D
Sbjct:    8 GLERFCSPGKGRGLRALQPFQVGDLLFSCPAYAYVLTVSERGNHCEFCFARKEGLSKCGRCKQAFYCNVECQREDWPMHKLECSPMVVFGENWNPS--ETVRLTARILAKQKIHPER----TPSEKLLAVKEFESHLDKLDNEKRDLIQSDIAALHHFYSKHLEFPDND 170          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy Swiss-Prot
Match: SMYD2_RAT (N-lysine methyltransferase SMYD2 OS=Rattus norvegicus GN=Smyd2 PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 6.478e-16
Identity = 39/91 (42.86%), Postives = 54/91 (59.34%), Query Frame = 3

Query: 1176 QFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCN 1448
            Q  CN  TI D EL  LG+ ++P ++++NHSC PN ++ ++G LA VRAVQ I    EV  SY++    T  R   L++ Y FTC C  C 
Sbjct:  178 QVNCNGFTIEDEELSHLGSAIFPDVALMNHSCCPNVIVTYKGTLAEVRAVQEIHPGDEVFTSYIDLLYPTEDRNDRLRDSYFFTCECRECT 268          

HSP 2 Score: 71.633 bits (174), Expect = 1.826e-11
Identity = 48/158 (30.38%), Postives = 75/158 (47.47%), Query Frame = 3

Query:  216 KGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFS-KSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDID-EKQLLLYAQMANLVNLI---LQLPN 674
            KGR L A R F+ G+++ S   Y CV         C+ CF+ K  L KC  C   +YC   CQK +W LH+LEC  +    +    S   T+R+  ++  ++K+  E     T  +    V    SH+  +D EK+ L+ + +A L       L+ P+
Sbjct:   17 KGRGLRALRPFHVGDLLFSCPAYACVLTVGERGHHCECCFARKEGLSKCGRCKQAFYCDVECQKEDWPLHKLECSSMVVFGENWNPS--ETVRLTARILAKQKMHPER----TPSEKLLAVREFESHLDKLDNEKKDLIQSDIAALHQFYSKHLEFPD 168          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy Swiss-Prot
Match: SMYD2_MOUSE (N-lysine methyltransferase SMYD2 OS=Mus musculus GN=Smyd2 PE=1 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 6.655e-16
Identity = 39/91 (42.86%), Postives = 54/91 (59.34%), Query Frame = 3

Query: 1176 QFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCN 1448
            Q  CN  TI D EL  LG+ ++P ++++NHSC PN ++ ++G LA VRAVQ I    EV  SY++    T  R   L++ Y FTC C  C 
Sbjct:  178 QVNCNGFTIEDEELSHLGSAIFPDVALMNHSCCPNVIVTYKGTLAEVRAVQEIHPGDEVFTSYIDLLYPTEDRNDRLRDSYFFTCECRECT 268          

HSP 2 Score: 72.4034 bits (176), Expect = 9.472e-12
Identity = 49/158 (31.01%), Postives = 76/158 (48.10%), Query Frame = 3

Query:  216 KGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFS-KSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDID-EKQLLLYAQMANLVNL---ILQLPN 674
            KGR L A R F+ G+++ S   Y CV         C+ CF+ K  L KC  C   +YC   CQK +W LH+LEC  +  L +    S   T+R+  ++  ++K+  E     T  +    V    SH+  +D EK+ L+ + +A L       L+ P+
Sbjct:   17 KGRGLRALRPFHVGDLLFSCPAYACVLTVGERGHHCECCFARKEGLSKCGRCKQAFYCDVECQKEDWPLHKLECSSMVVLGENWNPS--ETVRLTARILAKQKIHPER----TPSEKLLAVREFESHLDKLDNEKKDLIQSDIAALHQFYSKYLEFPD 168          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy TrEMBL
Match: A0A067K971_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_12048 PE=4 SV=1)

HSP 1 Score: 311.612 bits (797), Expect = 9.759e-91
Identity = 144/186 (77.42%), Postives = 162/186 (87.10%), Query Frame = 3

Query:  156 MEELQASLQPRGLTLSTFPDKGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFSKSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDIDEKQLLLYAQMANLVNLILQLPNIDIKEIAENFSKV 713
            M+ELQ +LQ  GLT+   P+KGR L   +DFYPGEVIISQEPYVCVPNNS TESRCDGCF+  NLKKCSAC VVWYCGS CQKLEWKLHRLEC++L+KLDK+RRKSVTP++R+MVKLYLRRKLQN   +  TA DNYNLVE LV+HM DIDEKQLLLYAQMANLVNLILQ P+I+IKEIAENFSK+
Sbjct:    1 MDELQEALQDWGLTVHNLPEKGRCLFTTKDFYPGEVIISQEPYVCVPNNSSTESRCDGCFTSVNLKKCSACQVVWYCGSKCQKLEWKLHRLECEVLSKLDKDRRKSVTPSVRLMVKLYLRRKLQNNKTLSATATDNYNLVEMLVAHMKDIDEKQLLLYAQMANLVNLILQWPDINIKEIAENFSKL 186          

HSP 2 Score: 265.388 bits (677), Expect = 2.560e-74
Identity = 121/158 (76.58%), Postives = 140/158 (88.61%), Query Frame = 3

Query: 1176 QFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCNKLGQYGDIQESAILEGYRCRDNGCSGFLLRESDEKGFVCQQCGLIRNKEEIRKIASDIKALSDKALK 1649
            + ACNAHTICDSELR LGTGLYPV+SIINHSCLPNAVLVFEGRLAVVRAVQHIPK +EV ISY+ETA ST+TRQK LK+QY FTC C RC K+GQ  D++ESAILEGYRC+D+ C GFLLR+SD+KGF CQQCGL R+KEE++KIA++I+A SDKA K
Sbjct:  185 KLACNAHTICDSELRPLGTGLYPVVSIINHSCLPNAVLVFEGRLAVVRAVQHIPKGAEVVISYIETAGSTMTRQKALKQQYFFTCTCSRCIKVGQQDDVRESAILEGYRCKDDRCIGFLLRDSDDKGFACQQCGLHRSKEEVKKIAAEIQATSDKASK 342          

HSP 3 Score: 94.7449 bits (234), Expect = 7.038e-17
Identity = 47/81 (58.02%), Postives = 60/81 (74.07%), Query Frame = 1

Query: 2836 PLKESLYKWFDSFTQFWIKKTSIFINDRLLGETDDAIKSFTKAVDILRITHGTSTPFMKELLMKLEEARVEASFKLSSRED 3078
            P+ +S+Y  F          +     + LLGET+DAI+S TKAVDILRITHGT++PFMKEL+MKLEEAR EAS+KLSS++D
Sbjct:  403 PVYQSVYPQFHPLLGLQYYTSGKL--EWLLGETEDAIRSLTKAVDILRITHGTNSPFMKELIMKLEEARAEASYKLSSKDD 481          

HSP 4 Score: 81.6481 bits (200), Expect = 1.067e-12
Identity = 31/41 (75.61%), Postives = 37/41 (90.24%), Query Frame = 1

Query: 2269 EEWKEALAICRLTIPVYERVYPGFHPLLGLQYYSCGKLEWL 2391
            E+W+EAL+ C+LTIPVY+ VYP FHPLLGLQYY+ GKLEWL
Sbjct:  389 EDWREALSYCKLTIPVYQSVYPQFHPLLGLQYYTSGKLEWL 429          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy TrEMBL
Match: E0CRB7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g03730 PE=4 SV=1)

HSP 1 Score: 302.368 bits (773), Expect = 1.968e-87
Identity = 139/186 (74.73%), Postives = 165/186 (88.71%), Query Frame = 3

Query:  156 MEELQASLQPRGLTLSTFPDKGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFSKSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDIDEKQLLLYAQMANLVNLILQLPNIDIKEIAENFSKV 713
            ME+LQA L   GLT+ST P+KGR LL+ +DF PGEVIISQEPYV VPNNS   SRC+GCF  SNLKKCSACHVVWYCGS+CQK +WKLHRLEC  L++L+KER+KS+TP+IR+MVKLY+RRKLQ+E ++P TA DNYNLVEALVSH++DIDEKQL+LYAQMANLVNLILQ P+I++KEIAENFSK+
Sbjct:    1 MEDLQADLSANGLTVSTTPEKGRCLLSIKDFSPGEVIISQEPYVSVPNNSAVHSRCEGCFRSSNLKKCSACHVVWYCGSTCQKSDWKLHRLECNALSRLEKERQKSLTPSIRLMVKLYMRRKLQSEKIMPTTARDNYNLVEALVSHITDIDEKQLVLYAQMANLVNLILQWPDINVKEIAENFSKL 186          

HSP 2 Score: 270.781 bits (691), Expect = 2.423e-76
Identity = 123/156 (78.85%), Postives = 140/156 (89.74%), Query Frame = 3

Query: 1176 QFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCNKLGQYGDIQESAILEGYRCRDNGCSGFLLRESDEKGFVCQQCGLIRNKEEIRKIASDIKALSDKA 1643
            + ACNAHTICD ELR LGTGLYPVISIINHSCLPN+VLVFE RLAVVRAVQHIPK +EV ISY+ETA STITRQK LKEQYLFTC C RC ++GQY DIQESAILEGYRC+D+ C GFLLR+SD+ GF+CQQCGL+RNKEEI+++AS++K LSDKA
Sbjct:  185 KLACNAHTICDGELRPLGTGLYPVISIINHSCLPNSVLVFEERLAVVRAVQHIPKGTEVLISYIETAGSTITRQKALKEQYLFTCTCPRCRRMGQYDDIQESAILEGYRCKDDRCDGFLLRDSDDIGFICQQCGLVRNKEEIKRLASELKPLSDKA 340          

HSP 3 Score: 88.1965 bits (217), Expect = 1.136e-14
Identity = 32/41 (78.05%), Postives = 38/41 (92.68%), Query Frame = 1

Query: 2269 EEWKEALAICRLTIPVYERVYPGFHPLLGLQYYSCGKLEWL 2391
            ++W+ AL  C+LTIPVY+RVYPGFHPLLGLQYY+CGKLEWL
Sbjct:  388 KDWRAALTYCKLTIPVYQRVYPGFHPLLGLQYYTCGKLEWL 428          

HSP 4 Score: 83.9593 bits (206), Expect = 1.952e-13
Identity = 40/52 (76.92%), Postives = 46/52 (88.46%), Query Frame = 1

Query: 2920 LLGETDDAIKSFTKAVDILRITHGTSTPFMKELLMKLEEARVEASFKLSSRE 3075
            LLGET+DA+KS TKA DIL+ITHGT+TPFMKELL KLEEAR EAS K SS++
Sbjct:  428 LLGETEDAVKSLTKAADILQITHGTNTPFMKELLFKLEEARAEASHKRSSKD 479          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy TrEMBL
Match: B9RJ97_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1032380 PE=4 SV=1)

HSP 1 Score: 297.36 bits (760), Expect = 1.497e-85
Identity = 138/186 (74.19%), Postives = 163/186 (87.63%), Query Frame = 3

Query:  156 MEELQASLQPRGLTLSTFPDKGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFSKSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDIDEKQLLLYAQMANLVNLILQLPNIDIKEIAENFSKV 713
            MEELQA+LQ  GL ++   +KGRSL+  +DF PGEVIISQEPYVCVPNNS TESRCD CFS SN+KKCSAC V WYCGSSCQKLEWKLHR+EC+ L+KLDK+RRK VTP+IR+MV+L +RRKLQ+E +I  TA DNY+LVEALV+HM DIDEKQL+LYAQMANLVNLIL+ P+++IKEIAENFSK+
Sbjct:    1 MEELQAALQNWGLRVANVTEKGRSLVTTKDFNPGEVIISQEPYVCVPNNSATESRCDRCFSSSNVKKCSACQVAWYCGSSCQKLEWKLHRIECEALSKLDKDRRKYVTPSIRLMVRLLIRRKLQSEKIISSTATDNYDLVEALVAHMKDIDEKQLVLYAQMANLVNLILRWPDVNIKEIAENFSKL 186          

HSP 2 Score: 263.462 bits (672), Expect = 1.061e-73
Identity = 120/158 (75.95%), Postives = 139/158 (87.97%), Query Frame = 3

Query: 1176 QFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCNKLGQYGDIQESAILEGYRCRDNGCSGFLLRESDEKGFVCQQCGLIRNKEEIRKIASDIKALSDKALK 1649
            + ACNAHTICDSELR LGTGLYPV+SIINHSCLPNAVLVF+GRLAVV  VQHIPK SEV ISY+ETA ST+TRQK LK+QY FTC C RC K+G   DIQESAILEGYRC+DN C+GFLLR+SD++GF+CQQCGL+R+KEE++K A++IKA SDKA K
Sbjct:  185 KLACNAHTICDSELRPLGTGLYPVVSIINHSCLPNAVLVFDGRLAVVHTVQHIPKGSEVLISYIETAGSTMTRQKALKQQYFFTCTCPRCIKMGLLDDIQESAILEGYRCKDNRCNGFLLRDSDDRGFICQQCGLLRSKEEVKKSAAEIKATSDKASK 342          

HSP 3 Score: 78.5666 bits (192), Expect = 1.178e-11
Identity = 33/41 (80.49%), Postives = 38/41 (92.68%), Query Frame = 1

Query: 2269 EEWKEALAICRLTIPVYERVYPGFHPLLGLQYYSCGKLEWL 2391
            E+W EAL+ C+LTIPVY+RVYP FHPLLGLQY+SCGKLEWL
Sbjct:  389 EDWGEALSYCKLTIPVYQRVYPEFHPLLGLQYFSCGKLEWL 429          

HSP 4 Score: 77.7962 bits (190), Expect = 2.112e-11
Identity = 36/52 (69.23%), Postives = 44/52 (84.62%), Query Frame = 1

Query: 2920 LLGETDDAIKSFTKAVDILRITHGTSTPFMKELLMKLEEARVEASFKLSSRE 3075
            LLG+T+ AIKS TKA+D+LRITHGT TPFMKEL+MKLEEAR E S+  +S +
Sbjct:  429 LLGDTEAAIKSLTKALDVLRITHGTKTPFMKELMMKLEEARAEVSYLRASND 480          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy TrEMBL
Match: M1BHD1_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400017559 PE=4 SV=1)

HSP 1 Score: 284.263 bits (726), Expect = 6.138e-85
Identity = 131/186 (70.43%), Postives = 163/186 (87.63%), Query Frame = 3

Query:  156 MEELQASLQPRGLTLSTFPDKGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFSKSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDIDEKQLLLYAQMANLVNLILQLPNIDIKEIAENFSKV 713
            MEEL+ +L  +GLT+S+ P+KGR L   RDF+PGEVIIS++PYV VPN S   ++C+ CF+ +NLK+CSAC VV YCG++CQKL+WKLHR+ECQ L+K+DKER KS+TP+IR+MVKLYLRRKLQ+E VIP+T MDNYNLVE+LVSHM+DIDEKQL+LYAQMANLV+LILQ P I++KEIAENFSKV
Sbjct:    1 MEELEEALSDKGLTVSSVPEKGRCLFTTRDFFPGEVIISEDPYVSVPNKS---AKCEWCFTSNNLKRCSACQVVCYCGNTCQKLDWKLHRVECQALSKVDKERVKSITPSIRLMVKLYLRRKLQDEKVIPITVMDNYNLVESLVSHMTDIDEKQLVLYAQMANLVSLILQWPEINVKEIAENFSKV 183          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy TrEMBL
Match: M5XD19_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005026mg PE=4 SV=1)

HSP 1 Score: 290.812 bits (743), Expect = 2.557e-83
Identity = 138/186 (74.19%), Postives = 157/186 (84.41%), Query Frame = 3

Query:  156 MEELQASLQPRGLTLSTFPDKGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFSKSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDIDEKQLLLYAQMANLVNLILQLPNIDIKEIAENFSKV 713
            MEELQ +L+ R LT+S  P+KGR L   RDF PGEVIISQEPYV VPNNS  ESRCD CF  SNLKKCSAC VV+YC +SCQK EWKLHRLEC+ L+KL KERR +VTP+IR+M+KLYLR KLQ E VIP +AMDNY LVEALV+HMS+IDEKQL+LYAQMANLV+LILQ P I+IKEIAENFSK+
Sbjct:    1 MEELQRALEDRSLTVSNVPEKGRCLFTTRDFSPGEVIISQEPYVSVPNNSSAESRCDACFESSNLKKCSACQVVYYCSNSCQKSEWKLHRLECEALSKLHKERRMAVTPSIRLMIKLYLRTKLQTERVIPASAMDNYKLVEALVAHMSEIDEKQLVLYAQMANLVSLILQWPGINIKEIAENFSKL 186          

HSP 2 Score: 272.322 bits (695), Expect = 9.309e-77
Identity = 122/156 (78.21%), Postives = 142/156 (91.03%), Query Frame = 3

Query: 1176 QFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCNKLGQYGDIQESAILEGYRCRDNGCSGFLLRESDEKGFVCQQCGLIRNKEEIRKIASDIKALSDKA 1643
            + ACNAHTICDSELR LGTGLYPVISI+NHSCLPN+VL+FEGR AVV AVQHIPK +EV ISY+ETA ST+TRQK LKEQYLFTC C RC+K+G+Y DIQESA+LEGYRC+DNGC GFLLRESD  GF+CQQCGL+R+KEEI++IAS++K+LSDKA
Sbjct:  185 KLACNAHTICDSELRPLGTGLYPVISIVNHSCLPNSVLLFEGRSAVVHAVQHIPKGAEVLISYIETAGSTLTRQKALKEQYLFTCTCPRCSKVGKYNDIQESAVLEGYRCKDNGCIGFLLRESDGNGFICQQCGLVRSKEEIKQIASELKSLSDKA 340          

HSP 3 Score: 82.0333 bits (201), Expect = 9.961e-13
Identity = 35/41 (85.37%), Postives = 38/41 (92.68%), Query Frame = 1

Query: 2269 EEWKEALAICRLTIPVYERVYPGFHPLLGLQYYSCGKLEWL 2391
            E+W EALA CRLTIPVY+RVYPG HPLLGLQYY+CGKLEWL
Sbjct:  389 EDWSEALAYCRLTIPVYQRVYPGCHPLLGLQYYTCGKLEWL 429          

HSP 4 Score: 80.8777 bits (198), Expect = 1.953e-12
Identity = 37/52 (71.15%), Postives = 46/52 (88.46%), Query Frame = 1

Query: 2920 LLGETDDAIKSFTKAVDILRITHGTSTPFMKELLMKLEEARVEASFKLSSRE 3075
            LLG+T++A+KS  KAVDIL+ITHGTSTPFMK+L ++LEEAR EAS+K SS E
Sbjct:  429 LLGDTENAVKSLIKAVDILQITHGTSTPFMKDLFVRLEEARAEASYKFSSEE 480          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy TrEMBL
Match: R0I272_9BRAS (Uncharacterized protein OS=Capsella rubella GN=CARUB_v10013566mg PE=4 SV=1)

HSP 1 Score: 290.426 bits (742), Expect = 4.344e-83
Identity = 130/185 (70.27%), Postives = 158/185 (85.41%), Query Frame = 3

Query:  156 MEELQASLQPRGLTLSTFPDKGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFSKSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDIDEKQLLLYAQMANLVNLILQLPNIDIKEIAENFSK 710
            M +L+  LQ R L++   P+KGRSL   RDF PGEVI+SQ+PY+CVPNN+ +ESRCDGCF  +NLK+CS C VVWYCGSSCQK EWKLHR EC+ L++LDKE+RK VTPTIR+MVKLY++R LQNE V+P+T  DNY+LV+ALVSHMS+IDEKQ+LLYAQMANLVNLILQ P +D++EIAENFSK
Sbjct:    6 MADLEMFLQDRCLSVLNLPEKGRSLFTTRDFRPGEVILSQKPYICVPNNTSSESRCDGCFKTNNLKRCSGCQVVWYCGSSCQKSEWKLHRDECKALSRLDKEKRKFVTPTIRLMVKLYIKRNLQNEKVLPITTTDNYSLVDALVSHMSEIDEKQMLLYAQMANLVNLILQFPTVDLREIAENFSK 190          

HSP 2 Score: 235.343 bits (599), Expect = 8.690e-64
Identity = 106/157 (67.52%), Postives = 135/157 (85.99%), Query Frame = 3

Query: 1176 QFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCNKLGQYGDIQESAILEGYRCRDNGCSGFLLRESDEKGFVCQQCGLIRNKEEIRKIASDIKALSDKAL 1646
            +F+CNAHTICDSELR  G GL+P +SIINHSC PNA+LVFE ++AVVRA+ +I KDSE++ISY+ETA ST+TRQK+L+EQYLF C C RCN +G+  DI+ESAILEGYRC +  C+ FLLR+ +EKGFVCQ+C L+R+KEE++KIASDIK +S+KAL
Sbjct:  190 KFSCNAHTICDSELRPQGIGLFPFVSIINHSCSPNAILVFEDQMAVVRAMDNILKDSEITISYIETAGSTLTRQKSLREQYLFHCHCARCNNVGKPDDIEESAILEGYRCANEKCTDFLLRDPEEKGFVCQKCLLLRSKEEVKKIASDIKRVSEKAL 346          

HSP 3 Score: 75.485 bits (184), Expect = 9.757e-11
Identity = 28/41 (68.29%), Postives = 34/41 (82.93%), Query Frame = 1

Query: 2269 EEWKEALAICRLTIPVYERVYPGFHPLLGLQYYSCGKLEWL 2391
            E W+EAL  CRL +PVY+RVYP  HPL+GLQ+Y+ GKLEWL
Sbjct:  394 ESWREALNYCRLIVPVYQRVYPATHPLIGLQFYTQGKLEWL 434          

HSP 4 Score: 74.3294 bits (181), Expect = 2.153e-10
Identity = 35/46 (76.09%), Postives = 39/46 (84.78%), Query Frame = 1

Query: 2920 LLGETDDAIKSFTKAVDILRITHGTSTPFMKELLMKLEEARVEASF 3057
            LLGET +A+ S TKA DILRI+HGTSTPFMKEL  KLEEAR EAS+
Sbjct:  434 LLGETKEAVSSLTKAFDILRISHGTSTPFMKELSAKLEEARAEASY 479          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy TrEMBL
Match: D7LAC4_ARALL (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_319740 PE=4 SV=1)

HSP 1 Score: 288.115 bits (736), Expect = 2.293e-82
Identity = 131/185 (70.81%), Postives = 156/185 (84.32%), Query Frame = 3

Query:  156 MEELQASLQPRGLTLSTFPDKGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFSKSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDIDEKQLLLYAQMANLVNLILQLPNIDIKEIAENFSK 710
            M +LQ  LQ R L++S  P KGRSL   RD  PGEVI+SQ+PY+CVPNN+  ESRCDGCF  +NLKKCS C VVWYCGS CQK EWKLHR EC+ L +L+KE+RK VTPTIR+MVKLY++R LQNE V+P+T+ DNY+LVEALVSHMS+IDEKQ+LLYAQMANLVNLILQ P++D++EIAENFSK
Sbjct:    1 MADLQRFLQDRCLSVSNLPHKGRSLFTTRDIRPGEVILSQKPYICVPNNTSLESRCDGCFKTNNLKKCSGCQVVWYCGSFCQKSEWKLHRHECKALTRLEKEKRKFVTPTIRLMVKLYIKRNLQNEKVLPITSTDNYSLVEALVSHMSEIDEKQMLLYAQMANLVNLILQFPSVDLREIAENFSK 185          

HSP 2 Score: 234.187 bits (596), Expect = 2.609e-63
Identity = 104/156 (66.67%), Postives = 136/156 (87.18%), Query Frame = 3

Query: 1176 QFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCNKLGQYGDIQESAILEGYRCRDNGCSGFLLRESDEKGFVCQQCGLIRNKEEIRKIASDIKALSDKA 1643
            +F+CNAH+ICDSELR  G GL+P++SIINHSC PNAVLVFE ++AVVRA+ +I KDSE++ISY+ETA ST+TRQK+LKEQYLF C C RC+ +G+  DI+ESAILEGYRC +  C+GFLLR+ +EKGFVCQ+C L+R+KEE++K+ASD+K +S+KA
Sbjct:  185 KFSCNAHSICDSELRPQGIGLFPLVSIINHSCSPNAVLVFEEQMAVVRAMDNISKDSEITISYIETAGSTLTRQKSLKEQYLFHCQCARCSNVGKPHDIEESAILEGYRCSNEKCTGFLLRDPEEKGFVCQKCLLLRSKEEVKKLASDLKTVSEKA 340          

HSP 3 Score: 77.0258 bits (188), Expect = 3.799e-11
Identity = 37/54 (68.52%), Postives = 43/54 (79.63%), Query Frame = 1

Query: 2914 DRLLGETDDAIKSFTKAVDILRITHGTSTPFMKELLMKLEEARVEASFKLSSRE 3075
            + LLGET +A+ S  KA DILRI+HGTSTPFMKEL  KLEEAR EAS+KL+  E
Sbjct:  427 EWLLGETKEAVSSLIKAFDILRISHGTSTPFMKELSAKLEEARAEASYKLACIE 480          

HSP 4 Score: 74.3294 bits (181), Expect = 2.322e-10
Identity = 28/41 (68.29%), Postives = 34/41 (82.93%), Query Frame = 1

Query: 2269 EEWKEALAICRLTIPVYERVYPGFHPLLGLQYYSCGKLEWL 2391
            E W+EAL  CRL +PVY+RVYP  HPL+GLQ+Y+ GKLEWL
Sbjct:  389 EIWREALNYCRLIVPVYQRVYPATHPLIGLQFYTQGKLEWL 429          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy TrEMBL
Match: M1BHD2_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400017559 PE=4 SV=1)

HSP 1 Score: 282.722 bits (722), Expect = 1.560e-80
Identity = 130/185 (70.27%), Postives = 162/185 (87.57%), Query Frame = 3

Query:  156 MEELQASLQPRGLTLSTFPDKGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFSKSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDIDEKQLLLYAQMANLVNLILQLPNIDIKEIAENFSK 710
            MEEL+ +L  +GLT+S+ P+KGR L   RDF+PGEVIIS++PYV VPN S   ++C+ CF+ +NLK+CSAC VV YCG++CQKL+WKLHR+ECQ L+K+DKER KS+TP+IR+MVKLYLRRKLQ+E VIP+T MDNYNLVE+LVSHM+DIDEKQL+LYAQMANLV+LILQ P I++KEIAENFSK
Sbjct:    1 MEELEEALSDKGLTVSSVPEKGRCLFTTRDFFPGEVIISEDPYVSVPNKS---AKCEWCFTSNNLKRCSACQVVCYCGNTCQKLDWKLHRVECQALSKVDKERVKSITPSIRLMVKLYLRRKLQDEKVIPITVMDNYNLVESLVSHMTDIDEKQLVLYAQMANLVSLILQWPEINVKEIAENFSK 182          

HSP 2 Score: 253.832 bits (647), Expect = 2.342e-70
Identity = 112/156 (71.79%), Postives = 136/156 (87.18%), Query Frame = 3

Query: 1176 QFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCNKLGQYGDIQESAILEGYRCRDNGCSGFLLRESDEKGFVCQQCGLIRNKEEIRKIASDIKALSDKA 1643
            +F+CNAHTICD+EL+ LGTGLYPV+SIINHSCLPN+VL+FEGR+AVVRAVQHIPK +EVSISY+E A +T TRQK LKEQYLF+C C+RC KLGQ  DIQESA+LEGY+C+D  CSGFLLR+S  KGF CQ CGL+R+KEEI+    +I++LS+KA
Sbjct:  182 KFSCNAHTICDAELKPLGTGLYPVVSIINHSCLPNSVLIFEGRMAVVRAVQHIPKGTEVSISYIEMAGTTATRQKALKEQYLFSCTCIRCIKLGQNDDIQESAVLEGYKCKDKKCSGFLLRDSGNKGFTCQLCGLVRDKEEIKNTVHEIQSLSEKA 337          

HSP 3 Score: 76.2554 bits (186), Expect = 6.413e-11
Identity = 32/40 (80.00%), Postives = 36/40 (90.00%), Query Frame = 1

Query: 2269 EEWKEALAICRLTIPVYERVYPGFHPLLGLQYYSCGKLEW 2388
            ++WKEAL  CRLTIPVY+RVYP  HPLLGLQYY+CGKLEW
Sbjct:  386 QDWKEALKYCRLTIPVYQRVYPECHPLLGLQYYTCGKLEW 425          

HSP 4 Score: 69.707 bits (169), Expect = 6.963e-9
Identity = 32/52 (61.54%), Postives = 43/52 (82.69%), Query Frame = 1

Query: 2923 LGETDDAIKSFTKAVDILRITHGTSTPFMKELLMKLEEARVEASFKLSSRED 3078
            LGET++A +S  KA ++LRITHGT+T FM+EL +KLEEAR E S+K SS+E+
Sbjct:  427 LGETEEAYRSLAKAAEVLRITHGTNTTFMEELFVKLEEARAELSYKSSSKEE 478          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy TrEMBL
Match: M4CD19_BRARP (Uncharacterized protein OS=Brassica rapa subsp. pekinensis GN=Bra002100 PE=4 SV=1)

HSP 1 Score: 282.722 bits (722), Expect = 1.855e-80
Identity = 133/185 (71.89%), Postives = 152/185 (82.16%), Query Frame = 3

Query:  156 MEELQASLQPRGLTLSTFPDKGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFSKSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDIDEKQLLLYAQMANLVNLILQLPNIDIKEIAENFSK 710
            M  LQ+ L+ R LT+   PDKGRSL  +RDF PGEVI+ QEPYV VPNN+ +ESRCDGCF    LKKCS C VVWYCGSSCQK EWKLHR EC+ L +L+KE+R  VTPTIR+MVKL L+R LQNE VIP+T  DNY+LVEALVSHMS++DEKQL+LYAQMANLVNLILQ P ID+KEIAENFSK
Sbjct:    1 MANLQSFLRDRCLTVLNLPDKGRSLFTSRDFRPGEVILRQEPYVSVPNNNSSESRCDGCFKTDGLKKCSGCQVVWYCGSSCQKSEWKLHRHECKALCRLEKEKRMLVTPTIRLMVKLCLKRNLQNEKVIPITTTDNYSLVEALVSHMSELDEKQLMLYAQMANLVNLILQFPGIDLKEIAENFSK 185          

HSP 2 Score: 235.343 bits (599), Expect = 1.004e-63
Identity = 105/157 (66.88%), Postives = 135/157 (85.99%), Query Frame = 3

Query: 1176 QFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCNKLGQYGDIQESAILEGYRCRDNGCSGFLLRESDEKGFVCQQCGLIRNKEEIRKIASDIKALSDKAL 1646
            +F+CNAH+ICDSELR  G GL+P++SIINHSC PNAVLVFE ++AVVRA+++I KDSEV+ISY+ETA ST+TRQK+LKEQYLF C C RC+ +G+  DI+ESAILEGYRC +  C+GFLLR+ D+KGFVCQ C L+R+KEE++K+A D+K +S KAL
Sbjct:  185 KFSCNAHSICDSELRPEGIGLFPLVSIINHSCSPNAVLVFEEKMAVVRAMEYISKDSEVTISYIETAGSTLTRQKSLKEQYLFHCQCARCSNIGKPHDIEESAILEGYRCANEKCNGFLLRDPDDKGFVCQNCMLLRSKEEVKKLAGDVKTVSAKAL 341          

HSP 3 Score: 75.0998 bits (183), Expect = 1.451e-10
Identity = 35/55 (63.64%), Postives = 45/55 (81.82%), Query Frame = 1

Query: 2914 DRLLGETDDAIKSFTKAVDILRITHGTSTPFMKELLMKLEEARVEASFKLSSRED 3078
            + LLG+T++A+ S  KA DILRI+HGTSTPFMKEL  KL+EAR EAS+KL + +D
Sbjct:  427 EWLLGQTEEAVSSLIKAYDILRISHGTSTPFMKELSAKLDEARAEASYKLLALKD 481          

HSP 4 Score: 73.559 bits (179), Expect = 3.980e-10
Identity = 27/41 (65.85%), Postives = 34/41 (82.93%), Query Frame = 1

Query: 2269 EEWKEALAICRLTIPVYERVYPGFHPLLGLQYYSCGKLEWL 2391
            E W+EAL  C+L +PVY+RVYP  HPL+GLQ+Y+ GKLEWL
Sbjct:  389 ESWREALDYCKLIVPVYQRVYPATHPLIGLQFYTQGKLEWL 429          

BLAST of g.barbadense_cottongen_reftransV1_0092647 vs. ExPASy TrEMBL
Match: A0A078HJV4_BRANA (BnaA07g02410D protein OS=Brassica napus GN=BnaA07g02410D PE=4 SV=1)

HSP 1 Score: 281.182 bits (718), Expect = 6.621e-80
Identity = 132/185 (71.35%), Postives = 152/185 (82.16%), Query Frame = 3

Query:  156 MEELQASLQPRGLTLSTFPDKGRSLLAARDFYPGEVIISQEPYVCVPNNSLTESRCDGCFSKSNLKKCSACHVVWYCGSSCQKLEWKLHRLECQLLAKLDKERRKSVTPTIRMMVKLYLRRKLQNENVIPVTAMDNYNLVEALVSHMSDIDEKQLLLYAQMANLVNLILQLPNIDIKEIAENFSK 710
            M  LQ+ L+ R LT+   PDKGRSL  +RDF PGEVI+ QEPYV VPNN+ +ESRCDGCF    LKKCS C VVWYCGSSCQK EWKLHR EC+ L +L+KE+R  VTPTIR+MVKL L+R LQNE VIP+T  D+Y+LVEALVSHMS++DEKQL+LYAQMANLVNLILQ P ID+KEIAENFSK
Sbjct:    1 MANLQSFLRDRCLTVLNLPDKGRSLFTSRDFRPGEVILRQEPYVSVPNNNSSESRCDGCFKTDGLKKCSGCQVVWYCGSSCQKSEWKLHRHECKALCRLEKEKRMLVTPTIRLMVKLCLKRNLQNEKVIPITTTDDYSLVEALVSHMSELDEKQLMLYAQMANLVNLILQFPGIDLKEIAENFSK 185          

HSP 2 Score: 232.261 bits (591), Expect = 9.950e-63
Identity = 104/157 (66.24%), Postives = 134/157 (85.35%), Query Frame = 3

Query: 1176 QFACNAHTICDSELRSLGTGLYPVISIINHSCLPNAVLVFEGRLAVVRAVQHIPKDSEVSISYVETAASTITRQKTLKEQYLFTCICVRCNKLGQYGDIQESAILEGYRCRDNGCSGFLLRESDEKGFVCQQCGLIRNKEEIRKIASDIKALSDKAL 1646
            +F+CNAH+ICDSELR  G GL+P++SIINHSC PNAVLVFE ++AVVRA+ +I +DSEV+ISY+ETA ST+TRQK+LKEQYLF C C RC+ +G+  DI+ESAILEGYRC +  C+GFLLR+ D+KGFVCQ C L+R+KEE++K+A D+K +S KAL
Sbjct:  185 KFSCNAHSICDSELRPEGIGLFPLVSIINHSCSPNAVLVFEEKMAVVRAMDNISEDSEVTISYIETAGSTLTRQKSLKEQYLFHCQCARCSNIGKPHDIEESAILEGYRCANEKCNGFLLRDPDDKGFVCQNCMLLRSKEEVKKLAGDVKTVSAKAL 341          

HSP 3 Score: 75.0998 bits (183), Expect = 1.503e-10
Identity = 35/53 (66.04%), Postives = 44/53 (83.02%), Query Frame = 1

Query: 2920 LLGETDDAIKSFTKAVDILRITHGTSTPFMKELLMKLEEARVEASFKLSSRED 3078
            LLG+T++A+ S  KA DILRI+HGTSTPFMKEL  KL+EAR EAS+KL + +D
Sbjct:  429 LLGQTEEAVSSLIKAYDILRISHGTSTPFMKELSAKLDEARAEASYKLLALKD 481          

HSP 4 Score: 73.9442 bits (180), Expect = 3.649e-10
Identity = 27/41 (65.85%), Postives = 34/41 (82.93%), Query Frame = 1

Query: 2269 EEWKEALAICRLTIPVYERVYPGFHPLLGLQYYSCGKLEWL 2391
            E W+EAL  C+L +PVY+RVYP  HPL+GLQ+Y+ GKLEWL
Sbjct:  389 ESWREALDYCKLIVPVYQRVYPATHPLIGLQFYTQGKLEWL 429