g.barbadense_cottongen_reftransV1_0092163, g.barbadense_cottongen_reftransV1_0092163 (contig) Gossypium barbadense

BLAST of g.barbadense_cottongen_reftransV1_0092163 vs. ExPASy TrEMBL
Match: B9I3G3_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0012s08930g PE=4 SV=2)

HSP 1 Score: 1084.32 bits (2803), Expect = 0.000e+0
Identity = 630/932 (67.60%), Postives = 750/932 (80.47%), Query Frame = 1

Query:  670 FIIYTVPPTAYITSSTPFTNALNVSVNISFTEXXXXXXXXXXXXVNDCNLLVYGAGQVVPSSLTVVEPNLKYLLLVSISSASRYGRLVLVMDTNFCTDAAGNPFARSQNSSFYVHYDQRNVFVDLRAHVPEKLLQLRSETRTIQATNNYNNLKVYLYFSAPXXXXXXXXXXXXXXXKGTLLPITGENHGNRRFGFMVXXXXXXXXXXXXFDANSTISRQGTLVSPVAPVTFLYDSQRTAVRLSTTCRMRTREHNIPISIKFMKPVFGFNSSLISISGGRLQSFHEISRSIYAAAIQADDDVVSVSVPENVTGDVAGNKNLASNVLRVRHYTIPXXXXXXXXXXTAIFLLTCFTAGLLTMSTASLQSVGAFXXXXXXXXXDPTRILFRSACHIQIFALSRWLPVTLPVEYYEFARSMEWSIPYFSLPWETGHVQPVMMGSSPTGTSNSFLSRTYDRVISRSFQ-----PNKIAAAVYGSPLTPVEYRSFFESQSIIPEAEYISDGLHSNGWRNFDRSMFWLAVIGGSXXXXXXXXXXXXKYKKRDSEKQGSYGALIFPRFEIFLVIVALPCICQAAAALIAGGTPXXXXXXXXXXXXXXXXXXXXXXXXXXGITFGKLLQYKEVHREGQQFHWYQELIRVTLGPGKRGQWTWKNEANSVYLTMFGPLFEDLRGPPKYMVSQISGSNLSRQGGPIIASDDETEDAEAPFIQKLFGILRIYYTMLESARRATLGILAGAYLNNNNTSSKTPIIILLCLTSFQLFFLVLKKPFIKKKVQLVEIISLSCEVAMFATCFVLLDKEFS----TTIGIFMLTLFLIGFLAQMSAEGYALYEQTKQLDTSENKFFTGLKIASVGFLLYFVPQKLMKSFESTFPV--FGLCMDTSTTGLPCDRN-------SSSSERAWPRQLREMAKASFSKDGSKAPTDP 3411
            + + T+PPTAYIT+S  FTNALNVSVNISFTE C+GGGGF CSSVN CNL+VYGAGQV+PSSLTV+EPNLKY LLV +     YGR+VLVMD NFCTDAAGN F R+ NSSF+VH D+R VFVDLR H+PEKLLQL +E RT++ATNNY+NLK YLYFS PILNSS EIL+SL+ S+G LLPI+GEN  NR+FGF VAN+S+IAIITIS  +NS ISR GT VSP+AP TFLYDSQR  VRLST    RT EH+IPISIKFMKPVFGFNSS +SI GG LQ FHEISRS Y A ++ADDDVVSVSVP+NVTGDVAGNKNL SN+L+VR +++P+ISSVIS F TA FL T   AGLLT+STASL S GAFSRPS  L+++PTR LFRSACHIQ+FALSRWL VTLP+EYYEFA+ ++WSIPYF LPWETG V P+M+ S+     NS++S+T+D  IS++ Q      NK ++ V+G PL P+EY SFFESQ+  PEAE+I D  HSNGWR+FDRSMFWLAVIG SL+LLH  L FI+K +KR ++KQ  YGAL FPRFEIFL ++ALPCIC+A+A+L+ GGT SG++VGILLLGVV FLLL+L L LS+GITFGKLLQYKE+H+EGQ FHWY+++ RVTLGPGKRGQWTWKN++NSVYL   GPLFEDLRGPPKYM+SQI+G    +QG  IIASDDETEDAEAPFIQKLFGILRIYYT+LES +R +LGI+AGAYL  +N SSKTP ++LL +T FQLFFLVLKKPFIKKKVQLVEIIS++ +V++FATCF+LL+K+ S    T +GIFM+ LFLIGFLAQM  E YALY Q K LD SE  F TGLK AS+GFLL+F+P++L ++ ES  P    G       TG   DRN       S   ++ W +QLRE+A+ASFSK+ S +  DP
Sbjct:  113 WTVDTIPPTAYITASKSFTNALNVSVNISFTEPCTGGGGFGCSSVNACNLIVYGAGQVIPSSLTVLEPNLKYTLLVGLYPNVFYGRVVLVMDKNFCTDAAGNRFTRAANSSFFVHLDRRRVFVDLRIHIPEKLLQLNNEIRTVKATNNYDNLKFYLYFSEPILNSSAEILNSLNTSEGVLLPISGENLMNRKFGFQVANLSSIAIITISLLSNSIISRPGTSVSPIAPSTFLYDSQRPVVRLSTLSNTRTGEHSIPISIKFMKPVFGFNSSFLSIGGGHLQGFHEISRSKYIAEVKADDDVVSVSVPQNVTGDVAGNKNLGSNILQVRRHSVPMISSVISAFATACFLATSLAAGLLTLSTASLLSAGAFSRPSPLLTAEPTRNLFRSACHIQVFALSRWLAVTLPIEYYEFAKGLQWSIPYFILPWETGGVHPIMVKSNSFSILNSYISKTHD--ISQNMQLEGKSGNK-SSPVFGLPLAPMEYISFFESQNFKPEAEHIIDPQHSNGWRDFDRSMFWLAVIGVSLVLLHVILLFIIKLRKRTADKQRDYGALTFPRFEIFLTVLALPCICKASASLVRGGTASGIIVGILLLGVVGFLLLALLLILSIGITFGKLLQYKEIHQEGQIFHWYRDITRVTLGPGKRGQWTWKNKSNSVYLIRLGPLFEDLRGPPKYMLSQIAGVP-RKQGDHIIASDDETEDAEAPFIQKLFGILRIYYTLLESVKRVSLGIVAGAYL--DNWSSKTPTVVLLSITFFQLFFLVLKKPFIKKKVQLVEIISITSQVSIFATCFILLEKKLSTREETRVGIFMILLFLIGFLAQMVNEWYALYRQIKWLDPSEQYFLTGLKTASIGFLLFFIPRRLSQNLESKLPARQHGDRETGGETGSSVDRNKISGSKGSGKPDKPWQKQLRELARASFSKERSGSQNDP 1038          

HSP 2 Score: 127.102 bits (318), Expect = 3.110e-26
Identity = 65/115 (56.52%), Postives = 81/115 (70.43%), Query Frame = 2

Query:  305 MGFFNMSCLIXXXXXXXXXXXKGNCDDSQFTLKFFKAPHPFSHLNSAKFVFQVLGAANV-TCSDCSITCKLDDGSASDCGGRKILYSGLQDGNHSFGVCVNGSQGAACTSYNWTV 646
            MG   +S L+LL      L F+  C+D++  +KF KAPH FSHLN A F FQVL   +V +C++CS +CKLD GS SDCG  K+ Y GLQDGNH+F VC+NGSQGA C +YNWTV
Sbjct:    1 MGRPKLSWLVLLCWALLFLCFRALCEDAEVFVKFLKAPHAFSHLNIATFEFQVLVGGDVNSCTNCSFSCKLDSGSESDCGATKVSYQGLQDGNHTFEVCINGSQGAGCATYNWTV 115          

BLAST of g.barbadense_cottongen_reftransV1_0092163 vs. ExPASy TrEMBL
Match: B9IEU0_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0015s09010g PE=4 SV=2)

HSP 1 Score: 1052.74 bits (2721), Expect = 0.000e+0
Identity = 591/880 (67.16%), Postives = 713/880 (81.02%), Query Frame = 1

Query:  814 NLLVYGAGQVVPSSLTVVEPNLKYLLLVSISSASRYGRLVLVMDTNFCTDAAGNPFARSQNSSFYVHYDQRNVFVDLRAHVPEKLLQLRSETRTIQATNNYNNLKVYLYFSAPXXXXXXXXXXXXXXXKGTLLPITGENHGNRRFGFMVXXXXXXXXXXXXFDANSTISRQGTLVSPVAPVTFLYDSQRTAVRLSTTCRMRTREHNIPISIKFMKPVFGFNSSLISISGGRLQSFHEISRSIYAAAIQADDDVVSVSVPENVTGDVAGNKNLASNVLRVRHYTIPXXXXXXXXXXTAIFLLTCFTAGLLTMSTASLQSVGAFXXXXXXXXXDPTRILFRSACHIQIFALSRWLPVTLPVEYYEFARSMEWSIPYFSLPWETGHVQPVMMGSSPTGTSNSFLSRTYDRVISRSFQPNKI--AAAVYGSPLTPVEYRSFFESQSIIPEAEYISDGLHSNGWRNFDRSMFWLAVIGGSXXXXXXXXXXXXKYKKRDSEKQGSYGALIFPRFEIFLVIVALPCICQAAAALIAGGTPXXXXXXXXXXXXXXXXXXXXXXXXXXGITFGKLLQYKEVHREGQQFHWYQELIRVTLGPGKRGQWTWKNEANSVYLTMFGPLFEDLRGPPKYMVSQISGSNLSRQGGPIIASDDETEDAEAPFIQKLFGILRIYYTMLESARRATLGILAGAYLNNNNTSSKTPIIILLCLTSFQLFFLVLKKPFIKKKVQLVEIISLSCEVAMFATCFVLLDKEFS----TTIGIFMLTLFLIGFLAQMSAEGYALYEQTKQLDTSENKFFTGLKIASVGFLLYFVPQKLMKSFESTFPVFGLCMDTST---TGLPCDRNSSSS-----ERAWPRQLREMAKASFSKDGSKAPTDP 3411
            +LLVYGAGQV+PSSLTV+EPNLKY LLV +S +  YGR++LVMD NFCTD AGN F R+ NSSF+VH D+R+VFVDLR H+PEKLLQL +E RT++ATNN +NLK Y+YFS PILNSS EIL+SL+ S+G LLPI+GEN GNR+FGF VAN+S+IA++TI   +NS ISR GT VSP+APVTFLYDSQR AVRLST+   RT+EH+IPISIKF+KPVFGFNSS +SISGG LQ FHEISRS Y A I+ADDD++SVS+P+NV GDVAGNKNLASN+L+VRHY++P ISSVIS F TA FL T   AGLLT+STASL S GAFSRPSS L+++PTR +FR+ACHIQ+FA+SRWL VTLPVEYYEFAR+++WSIPYFSLPWETG +QP+M+ S+ +  ++S++S+T+D  +S   +   +  ++ VYG PL+P+EY SFFESQS  PEAE++ D  HSNGWR+FDRSMFWLAVIGGS+ILLHA L FILK +K ++EKQ  YGAL  PRFEIFL  +ALPCIC A+AAL+ GGT SG++VGILLLGVV F+LL+LFL LS+GITFGKLLQYKEVH+EGQ FHWYQ++IRVTLGPGKRGQWTWKN+  SVYL   G LFEDLRGPPKYM+SQI+G     QG  IIASDDETEDAEAPFIQKLFG+LRIYYT+LES +R +LGILAG YL  ++ SSKTP ++LL +T FQLFFLVLKKPFIKKKVQLVEIIS+SC+V +FATCF+LL+KE S    T +GIFM+ LFLIGFLAQM+ E YALY Q  +LD SE  F TGLK AS+GFLL F+ + L +  ES  P      D  T    G   DRN SS      ++ W +QLRE+A+ASF+K+ S +  DP
Sbjct:   17 HLLVYGAGQVIPSSLTVLEPNLKYTLLVGLSPSVLYGRVILVMDKNFCTDTAGNRFTRAANSSFFVHVDRRSVFVDLRIHIPEKLLQLNNEIRTVKATNNDDNLKFYMYFSEPILNSSAEILNSLNTSQGVLLPISGENLGNRKFGFQVANLSSIAVVTIGLLSNSIISRPGTSVSPIAPVTFLYDSQRPAVRLSTSSNTRTKEHSIPISIKFVKPVFGFNSSFLSISGGHLQGFHEISRSKYIAEIKADDDILSVSIPQNVIGDVAGNKNLASNILQVRHYSVPTISSVISAFATACFLATSLAAGLLTLSTASLLSAGAFSRPSSLLTAEPTRNIFRTACHIQVFAMSRWLAVTLPVEYYEFARNLQWSIPYFSLPWETGDIQPIMVKSNSSSGAHSYISKTHDISLSMQLKGKSVNKSSPVYGLPLSPMEYLSFFESQSFKPEAEHVLDPQHSNGWRDFDRSMFWLAVIGGSMILLHAILLFILKLRKGNTEKQRDYGALTLPRFEIFLTFLALPCICVASAALVRGGTTSGIIVGILLLGVVGFILLALFLILSIGITFGKLLQYKEVHQEGQIFHWYQDIIRVTLGPGKRGQWTWKNQPKSVYLVRLGALFEDLRGPPKYMLSQIAGVP-RNQGDRIIASDDETEDAEAPFIQKLFGVLRIYYTLLESVKRVSLGILAGVYL--DSWSSKTPTVVLLSITCFQLFFLVLKKPFIKKKVQLVEIISISCQVCIFATCFILLEKELSTGVETKVGIFMIALFLIGFLAQMANEWYALYRQIMRLDPSEKYFLTGLKTASIGFLLLFISKGLSQDLESKLPAKRRS-DGGTGGEAGSSVDRNKSSGSPGTPDKPWQKQLRELARASFTKERSGSRNDP 892          

BLAST of g.barbadense_cottongen_reftransV1_0092163 vs. ExPASy TrEMBL
Match: G7J5D6_MEDTR (Transmembrane protein, putative OS=Medicago truncatula GN=MTR_3g077550 PE=4 SV=2)

HSP 1 Score: 1036.56 bits (2679), Expect = 0.000e+0
Identity = 586/926 (63.28%), Postives = 721/926 (77.86%), Query Frame = 1

Query:  682 TVPPTAYITSSTPFTNALNVSVNISFTEXXXXXXXXXXXXVNDCNLLVYGAGQVVPSSLTVVEPNLKYLLLVSISSASRYGRLVLVMDTNFCTDAAGNPFARSQNSSFYVHYDQRNVFVDLRAHVPEKLLQLRSETRTIQATNNYNNLKVYLYFSAPXXXXXXXXXXXXXXXKGTLLPITGENHGNRRFGFMVXXXXXXXXXXXXFDANSTISRQGTLVSPVAPVTFLYDSQRTAVRLSTTCRMRTREHNIPISIKFMKPVFGFNSSLISISGGRLQSFHEISRSIYAAAIQADDDVVSVSVPENVTGDVAGNKNLASNVLRVRHYTIPXXXXXXXXXXTAIFLLTCFTAGLLTMSTASLQSVGAFXXXXXXXXXDPTRILFRSACHIQIFALSRWLPVTLPVEYYEFARSMEWSIPYFSLPWETGHVQPVMMGSSPTGTSNSF---LSRTYDRVISRSFQPNKIAAAVYGSPLTPVEYRSFFESQSIIPEAEYISDGLHSNGWRNFDRSMFWLAVIGGSXXXXXXXXXXXXKYKKRDSEKQGSYGALIFPRFEIFLVIVALPCICQAAAALIAGGTPXXXXXXXXXXXXXXXXXXXXXXXXXXGITFGKLLQYKEVHREGQQFHWYQELIRVTLGPGKRGQWTWKNEANSVYLTMFGPLFEDLRGPPKYMVSQISGSNLSRQGGPIIASDDETEDAEAPFIQKLFGILRIYYTMLESARRATLGILAGAYLNNNNTSSKTPIIILLCLTSFQLFFLVLKKPFIKKKVQLVEIISLSCEVAMFATCFVLLDKEFS----TTIGIFMLTLFLIGFLAQMSAEGYALYEQTKQLDTSENKFFTGLKIASVGFLLYFVPQKLMKSFESTFPVFGLC-MDTSTTGLPCDRNSSSSERA-------WPRQLREMAKASFSKDGSKAP-TDP 3411
            T+PPTAY+T++T FT++LNVSVNISF+E C G G    S VN CNLLVYGAGQV+PSS  +++PNL Y LLVS+SS  +Y R +LVMD NFCTD AGN F R  NSS Y+H D+R V+V++R HVPEKL+Q+ SETRT+QATN++N LKVYLYFSAP+LNSSTEI++SL +S+G+LLP + EN GNRRFGFM+ANIS+ AII+++F++ S I+RQGT VSP APV FLYDS+R AV LST  RMRT++HNI I I+F+KPVFGFN+S ISISGG L+SFH++  S Y   +QADDD V VSVPENVT DVAGNKNLASNVL+VRHY++P+ISSVIS F TA F LT   AGLLT+STASLQSV  F+R SS L  DP R LFR  CHIQ+FAL+RWL V  PVE+YEF+R ++W+IP FS+PWE+G +   M+GSSP G+S+S    L+   + ++ ++       A+VYGSPLT  EY+ +FES ++ PEAEYI D  HS+GW +F R+MFWLAVI GS ++LH FL  ILK++KR+SEK G+YGAL+FPRFEIFL+ +ALP IC+A+  LI GG P+ + VGI+LL  V+ +LL+LF+FLSVGITFGKLLQYKEVH EG+ FHWYQELIRVTLGPGKRGQWTWK +A SVYLT+FGPLFEDLRGPPKYM+SQISG +   Q   II SDDETEDAEAPFIQKLFGILRIY+  LES RR +LGILAG +++  + SSK+PIII+L +TSF LFF+VLKKPFIKKKVQLVEIISL+CEVA FATCFVLL K+FS    T  GIFML LFL+G+ +Q++ E YALY QTK LD  E   F GLK+AS+GF+LYF+PQK +K+ E   P  G    +T    L  +R   S  R+       W +++RE+AK SFSKD S    TDP
Sbjct:  118 TIPPTAYVTAATSFTSSLNVSVNISFSEPCIGEGFRCKS-VNACNLLVYGAGQVIPSSFKILKPNLMYSLLVSLSSTVQYSRAILVMDKNFCTDIAGNSFTRMPNSSVYIHIDRRKVYVNIRTHVPEKLVQINSETRTVQATNDHNKLKVYLYFSAPVLNSSTEIMNSLKVSQGSLLPTSAENLGNRRFGFMIANISSTAIISVNFNSKSIITRQGTQVSPNAPVNFLYDSKRPAVMLSTH-RMRTKDHNIQILIEFVKPVFGFNTSCISISGGLLKSFHKLKWSTYIVELQADDDFVFVSVPENVTHDVAGNKNLASNVLQVRHYSVPLISSVISAFATATFGLTSIAAGLLTISTASLQSVDTFTRSSSFLIVDPARNLFRILCHIQVFALARWLSVKWPVEFYEFSRHLQWTIPSFSVPWESGPMSLFMVGSSPFGSSSSSAKALATIPNMLLGQNLN---YGASVYGSPLTSSEYQQYFESTNMKPEAEYILDSQHSSGWTDFYRTMFWLAVICGSFMVLHGFLLIILKFRKRNSEKNGTYGALVFPRFEIFLLFLALPGICKASTGLIRGGAPAAMAVGIILLIFVSTVLLALFMFLSVGITFGKLLQYKEVHHEGETFHWYQELIRVTLGPGKRGQWTWKEKAKSVYLTIFGPLFEDLRGPPKYMLSQISGGSQPSQNDHIIVSDDETEDAEAPFIQKLFGILRIYFVFLESIRRVSLGILAGVFIHTRSQSSKSPIIIMLSITSFMLFFMVLKKPFIKKKVQLVEIISLTCEVAFFATCFVLLKKDFSVRTETKFGIFMLVLFLVGYCSQIANEWYALYAQTKLLDPEEKSLFRGLKVASIGFVLYFIPQKWIKNLEKKLPQNGHANSETRDNALIAERCMHSGSRSSGTPDIPWLKRVRELAKGSFSKDRSGVQITDP 1038          

HSP 2 Score: 105.916 bits (263), Expect = 1.171e-19
Identity = 51/93 (54.84%), Postives = 66/93 (70.97%), Query Frame = 2

Query:  374 NCDDSQFTLKFFKAPHPFSHLNSAKFVFQVLGA-ANVTCSDCSITCKLDDGSASDCGGRKILYSGLQDGNHSFGVCVNGSQGA-ACTSYNWTV 646
             C  S  T+KF KAPH FSHLNSA F F+VL + +N +C++CS++CKLD+G  S C   ++ YS L+DGNH+F VC NG QG   C S+NWTV
Sbjct:   24 KCGGSDVTVKFLKAPHAFSHLNSATFAFEVLNSGSNRSCANCSLSCKLDNGIRSVCTNGRVTYSSLKDGNHTFEVCTNGHQGLFGCASHNWTV 116          

BLAST of g.barbadense_cottongen_reftransV1_0092163 vs. ExPASy TrEMBL
Match: M5WE80_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa000610mg PE=4 SV=1)

HSP 1 Score: 1033.09 bits (2670), Expect = 0.000e+0
Identity = 599/949 (63.12%), Postives = 739/949 (77.87%), Query Frame = 1

Query:  637 LDCWLRNLPLFFIIYTVPPTAYITSSTPFTNALNVSVNISFTEXXXXXXXXXXXXVNDCNLLVYGAGQVVPSSLTVVEPNLKYLLLVSISSASRYGRLVLVMDTNFCTDAAGNPFARSQNSSFYVHYDQRNVFVDLRAHVPEKLLQLRSETRTIQATNNYNNLKVYLYFSAPXXXXXXXXXXXXXXXKGTLLPITGENHGNRRFGFMVXXXXXXXXXXXXFDANSTISRQGTLVSPVAPVTFLYDSQRTAVRLSTTCRMRTREHNIPISIKFMKPVFGFNSSLISISGGRLQSFHEISRSIYAAAIQADDDVVSVSVPENVTGDVAGNKNLASNVLRVRHYTIPXXXXXXXXXXTAIFLLTCFTAGLLTMSTASLQSVGAFXXXXXXXXXDPTRILFRSACHIQIFALSRWLPVTLPVEYYEFARSMEWSIPYFSLPWETGHVQPVMMGSSPTGTSNSFLSRTYDRVISRSFQPNK----IAAAVYGSPLTPVEYRSFFE--SQSIIPEAEYISDGLHSNGWRNFDRSMFWLAVIGGSXXXXXXXXXXXXKYKKRDSEKQGSYGALIFPRFEIFLVIVALPCICQAAAALIAGGTPXXXXXXXXXXXXXXXXXXXXXXXXXXGITFGKLLQYKEVHREGQQFHWYQELIRVTLGPGKRGQWTWKNEANSVYLTMFGPLFEDLRGPPKYMVSQISGSNLSRQGGPIIASDDETEDAEAPFIQKLFGILRIYYTMLESARRATLGILAGAYLNNNNTSSKTPIIILLCLTSFQLFFLVLKKPFIKKKVQLVEIISLSCEVAMFATCFVLLDKEFS----TTIGIFMLTLFLIGFLAQMSAEGYALYEQTKQLDTSENKFFTGLKIASVGFLLYFVPQKLMKSFESTFPV----------FGLCMD-TSTTG---LPCDRNSSSSERAWPRQLREMAKASFSKDGSKAPTDP 3411
            + C L N    + + TVPPTA +T+S  FT+ALNVSVNISF+ESCSGGGGF C S N CNLLVYGAGQV+PSSL++++PNLKY LLV +SS+ +YGR+VLVMD NFCTD AGN F R++NS FYV  D+RNVFV+LR H+PE+LLQL  ETRT+QATNNYNNLK+Y+YFS P+LNSS +IL+SL+IS+G+LLP +G N GNRRFGF+VAN+S+IAIIT+S ++N  ISRQGT VSP++P TFL+DS+R  V+LSTT  MRTREHNIPI IKFMKPVFGFNSS +S+S                   +A DD+VSV+VPEN++GDVAGNKNLASN+LRV HY++P+I+SVIS F TA+F++T   AGLL++STASLQS+ +F    SSL+SDP R LFR ACHIQ+FALSRWL VTLPV YYE  R + WSIPYFSLPWE G+  PV    +   +S+S+++  +   + +S Q  K       ++YG PLTP EYR+FFE  S++I PEA+YIS+   SN WRNFDR+MFWLAVI G+LILLH  + FILK +K++SEKQ SYGAL FPRFEIFLVI+ALPCI +A+AAL+ GG PSGV+VG  LL + +FLLL+L  FLSVGITFGKLLQYKEVHREG +FHWYQEL+RVTLGPGKRGQWTWK + NSVYL +FGPLFEDLRGPPKYM+SQISG N  + G  IIASDDETEDAEAPFIQK+FGILRIYYT+LE  +R  +G++AG Y+  +  SSKTP + LLC+TSFQLFFLVLKKPFIKKKVQLVEIIS+S EV +FATC VLL+KEFS    T +GIFML LFL+G++AQM  E YALY+QT  LD++E  F TGLK+AS+G LL  +PQ+L+K+ E  F V           G   D +S+TG      +R+ + +++ W +QLRE+AK+SFSK+GS    DP
Sbjct:  106 IGCALHN----WTVDTVPPTASVTASRSFTDALNVSVNISFSESCSGGGGFGCLSANTCNLLVYGAGQVIPSSLSILQPNLKYSLLVGLSSSVQYGRVVLVMDKNFCTDIAGNRFVRTENSRFYVRIDRRNVFVNLRIHIPERLLQLNGETRTVQATNNYNNLKIYVYFSEPVLNSSAQILNSLNISQGSLLPASGNNTGNRRFGFVVANMSSIAIITVSLNSNLIISRQGTPVSPISPATFLFDSKRPTVKLSTTSSMRTREHNIPILIKFMKPVFGFNSSSLSLS-------------------EAVDDMVSVNVPENISGDVAGNKNLASNILRVTHYSVPLIASVISAFATALFVVTSLAAGLLSISTASLQSLWSF-SRPSSLASDPARNLFRIACHIQVFALSRWLAVTLPVNYYELVRGLRWSIPYFSLPWEAGNGFPVSSPFT---SSSSYMTENHGSEVFQSKQLEKEIFSKDTSLYGLPLTPTEYRTFFELDSENIKPEADYISNPYSSNRWRNFDRTMFWLAVICGTLILLHVLVLFILKMRKKNSEKQSSYGALTFPRFEIFLVILALPCIYEASAALVRGGMPSGVIVGSSLLVITSFLLLALLFFLSVGITFGKLLQYKEVHREGLEFHWYQELVRVTLGPGKRGQWTWKGQPNSVYLIIFGPLFEDLRGPPKYMLSQISGGNPQKHGESIIASDDETEDAEAPFIQKVFGILRIYYTLLECLKRVAVGVMAGVYM--DKWSSKTPSVALLCITSFQLFFLVLKKPFIKKKVQLVEIISISSEVGLFATCVVLLEKEFSAGDKTKVGIFMLFLFLVGYVAQMINEWYALYKQTLLLDSAEKSFLTGLKLASIGCLLLIIPQRLIKTLEKKFQVESKFQVTQNAEGATRDPSSSTGGYRGSGNRSLAGTDKPWLKQLRELAKSSFSKEGSGVTNDP 1025          

HSP 2 Score: 124.79 bits (312), Expect = 1.457e-25
Identity = 64/115 (55.65%), Postives = 80/115 (69.57%), Query Frame = 2

Query:  305 MGFFNMSCLIXXXXXXXXXXXKGNCDDSQFTLKFFKAPHPFSHLNSAKFVFQVLGAANV-TCSDCSITCKLDDGSASDCGGRKILYSGLQDGNHSFGVCVNGSQGAACTSYNWTV 646
            MG  + S  +LL  VF +L  K + D S+ ++KFFKAPH FSHLNSA F F+ L   N  +C++CS +CKLDDG  S+CG RK+ YSGLQDGNH+F VC NG QG  C  +NWTV
Sbjct:    1 MGLLHTSWAVLLLWVFPLLLLKAHSDGSEVSVKFFKAPHAFSHLNSATFAFEALVGGNAASCTNCSFSCKLDDGMGSNCGTRKVSYSGLQDGNHTFEVCTNGPQGIGCALHNWTV 115          

BLAST of g.barbadense_cottongen_reftransV1_0092163 vs. ExPASy TrEMBL
Match: K4BKC9_SOLLC (Uncharacterized protein OS=Solanum lycopersicum GN=Solyc03g112760.2 PE=4 SV=1)

HSP 1 Score: 1025.39 bits (2650), Expect = 0.000e+0
Identity = 571/925 (61.73%), Postives = 724/925 (78.27%), Query Frame = 1

Query:  670 FIIYTVPPTAYITSSTPFTNALNVSVNISFTEXXXXXXXXXXXXVNDCNLLVYGAGQVVPSSLTVVEPNLKYLLLVSISSASRYGRLVLVMDTNFCTDAAGNPFARSQNSSFYVHYDQRNVFVDLRAHVPEKLLQLRSETRTIQATNNYNNLKVYLYFSAPXXXXXXXXXXXXXXXKGTLLPITGENHGNRRFGFMVXXXXXXXXXXXXFDANSTISRQGTLVSPVAPVTFLYDSQRTAVRLSTTCRMRTREHNIPISIKFMKPVFGFNSSLISISGGRLQSFHEISRSIYAAAIQADDDVVSVSVPENVTGDVAGNKNLASNVLRVRHYTIPXXXXXXXXXXTAIFLLTCFTAGLLTMSTASLQSVGAFXXXXXXXXXDPTRILFRSACHIQIFALSRWLPVTLPVEYYEFARSMEWSIPYFSLPWETGHVQPVMMGSSPTGTSNSFLSRTYDRVIS-RSFQPNKIAAAVYGSPLTPVEYRSFFESQSIIPEAEYISDGLHSNGWRNFDRSMFWLAVIGGSXXXXXXXXXXXXKYKKRDSEKQGSYGALIFPRFEIFLVIVALPCICQAAAALIAGGTPXXXXXXXXXXXXXXXXXXXXXXXXXXGITFGKLLQYKEVHREGQQFHWYQELIRVTLGPGKRGQWTWKNEANSVYLTMFGPLFEDLRGPPKYMVSQISGSNLSRQGGPIIASDDETEDAEAPFIQKLFGILRIYYTMLESARRATLGILAGAYLNNNNTSSKTPIIILLCLTSFQLFFLVLKKPFIKKKVQLVEIISLSCEVAMFATCFVLLDKEFS---TTIGIFMLTLFLIGFLAQMSAEGYALYEQTKQLDTSENKFFTGLKIASVGFLLYFVPQKLMKSFESTFPVFGLCM-DTSTTGLPCDRN------SSSSERAWPRQLREMAKASFSKDGSKAPTDP 3411
            + + T+ PTAYIT+ T FTNA NVSV+I+FTE C   GGF C+S N CNLLVYGAGQVVP++L V+EP+LK+ ++VS+S+  +YGR++LVMD NFC+D AGN F R+ NSS ++H+D+R VF D+R H+PE+LLQ+ SE RT++ATN+  N++VYLYF+ PI NSST+IL+SLSIS+G L PI+G + G RRFGF V  IS  AI+T+S  ++  +S QGT ++PVAPVTFLYD QR AVRLSTT RMRT +  IP+ IKF+KPVFGFNSS +SISGG+LQSF E+SRS+Y   IQA DD VSVS+PENVTGDVAGN NL SNVLR++HYT+PV S V+SI  T+ F++T F AGLLT+STASLQSVGA+SRPSS ++SDP R LFR ACHIQ FAL+RWLP+TLPVEYYEFAR ++WS+PYFSLPWE G +   MMG   T   +S+ S+  D  +    +  NK AAA+YG PL+P+EYRS F SQ ++PEA+YI D  +SNGWR+F+RSMFWLAVIGGSLILLHA + F+L+ +K D EK+ SYGAL+FPRFEIFL I+A+PCIC+A+  ++ GG+ +G+ VGILLLG+V+FLLL LFLFLS+GIT GKLLQYKEVH+ GQ+FHWY+EL+RVTLGPGKRGQWTW+N  +S Y+ MFGPLFEDLRGPPKYM+SQI+  N ++    +IA+DDETEDAEAPFIQKLFGILRIY+T LE  +R  LGI+AG YL   + SSK+PI++LL +TSFQLFF++LKKPFIKKKVQLVEIIS++CE  +FA C  L+ +  S   T IGI M+ LF I FLAQ+  E YALY QTK+L   +  F +GLK AS+GFLL+F+PQ+L++  ES   +    + +T      CDRN      SS ++R + RQ RE+AK+SFSKD +   +DP
Sbjct:  114 WTVDTIAPTAYITTPTSFTNASNVSVDITFTEPCWSQGGFGCASTNSCNLLVYGAGQVVPNTLNVIEPDLKFSVVVSLSTRDQYGRVILVMDKNFCSDPAGNKFKRTDNSSLFIHFDRRTVFADIRTHIPERLLQIDSEMRTVRATNSTENMEVYLYFNEPISNSSTDILNSLSISQGLLTPISGNSFGERRFGFQVRGISQTAIVTLSVRSDLILSWQGTPIAPVAPVTFLYDMQRPAVRLSTTSRMRTCDEQIPVLIKFVKPVFGFNSSHVSISGGQLQSFQEMSRSMYTVNIQARDDFVSVSIPENVTGDVAGNMNLQSNVLRLKHYTVPVTSEVLSILATSAFVVTSFAAGLLTVSTASLQSVGAYSRPSSLMTSDPARNLFRIACHIQFFALTRWLPITLPVEYYEFARGLQWSVPYFSLPWEMGSMHQFMMGPGSTTDPHSYSSKINDFGMKPGKYNVNK-AAALYGLPLSPMEYRSIFGSQDLLPEAQYIVDPKYSNGWRDFNRSMFWLAVIGGSLILLHALVLFVLRLRK-DREKKWSYGALVFPRFEIFLTILAIPCICKASVNVVKGGSSAGIAVGILLLGLVSFLLLQLFLFLSIGITLGKLLQYKEVHQVGQKFHWYEELVRVTLGPGKRGQWTWRNSRDSRYVVMFGPLFEDLRGPPKYMLSQIAVGNPNKHPDKVIATDDETEDAEAPFIQKLFGILRIYFTFLEFVKRVCLGIVAGTYL--KSLSSKSPIVVLLTITSFQLFFMLLKKPFIKKKVQLVEIISVACETGIFAACIALIGRNSSRNETAIGITMIALFFIAFLAQLVNEWYALYRQTKRLGAEDKSFCSGLKAASIGFLLFFIPQRLIRKLESGSALLDRVLKETGDVTSSCDRNRSSGSRSSGTDRPFTRQFRELAKSSFSKDSNVTTSDP 1034          

HSP 2 Score: 90.8929 bits (224), Expect = 4.603e-15
Identity = 45/91 (49.45%), Postives = 53/91 (58.24%), Query Frame = 2

Query:  377 CDDSQFTLKFFKAPHPFSHLNSAKFVFQVLGAAN-VTCSDCSITCKLDDGSASDCGGRKILYSGLQDGNHSFGVCVNGSQGAACTSYNWTV 646
            CD S  TLK    P  F++ N A F FQVL   N   C+DCS +CKLDD     C G ++ Y+ L DG H+F VC NGS G  C  YNWTV
Sbjct:   26 CDGSDVTLKLLTTPREFTNRNFANFAFQVLAGGNGDICADCSTSCKLDDYVPVVCEGGEVSYTQLLDGKHTFEVCTNGSLGVGCAHYNWTV 116          

BLAST of g.barbadense_cottongen_reftransV1_0092163 vs. ExPASy TrEMBL
Match: M1BJK8_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400018158 PE=4 SV=1)

HSP 1 Score: 1023.08 bits (2644), Expect = 0.000e+0
Identity = 570/925 (61.62%), Postives = 724/925 (78.27%), Query Frame = 1

Query:  670 FIIYTVPPTAYITSSTPFTNALNVSVNISFTEXXXXXXXXXXXXVNDCNLLVYGAGQVVPSSLTVVEPNLKYLLLVSISSASRYGRLVLVMDTNFCTDAAGNPFARSQNSSFYVHYDQRNVFVDLRAHVPEKLLQLRSETRTIQATNNYNNLKVYLYFSAPXXXXXXXXXXXXXXXKGTLLPITGENHGNRRFGFMVXXXXXXXXXXXXFDANSTISRQGTLVSPVAPVTFLYDSQRTAVRLSTTCRMRTREHNIPISIKFMKPVFGFNSSLISISGGRLQSFHEISRSIYAAAIQADDDVVSVSVPENVTGDVAGNKNLASNVLRVRHYTIPXXXXXXXXXXTAIFLLTCFTAGLLTMSTASLQSVGAFXXXXXXXXXDPTRILFRSACHIQIFALSRWLPVTLPVEYYEFARSMEWSIPYFSLPWETGHVQPVMMGSSPTGTSNSFLSRTYDRVIS-RSFQPNKIAAAVYGSPLTPVEYRSFFESQSIIPEAEYISDGLHSNGWRNFDRSMFWLAVIGGSXXXXXXXXXXXXKYKKRDSEKQGSYGALIFPRFEIFLVIVALPCICQAAAALIAGGTPXXXXXXXXXXXXXXXXXXXXXXXXXXGITFGKLLQYKEVHREGQQFHWYQELIRVTLGPGKRGQWTWKNEANSVYLTMFGPLFEDLRGPPKYMVSQISGSNLSRQGGPIIASDDETEDAEAPFIQKLFGILRIYYTMLESARRATLGILAGAYLNNNNTSSKTPIIILLCLTSFQLFFLVLKKPFIKKKVQLVEIISLSCEVAMFATCFVLLDKEFS---TTIGIFMLTLFLIGFLAQMSAEGYALYEQTKQLDTSENKFFTGLKIASVGFLLYFVPQKLMKSFESTFPVFGLCM-DTSTTGLPCDRN------SSSSERAWPRQLREMAKASFSKDGSKAPTDP 3411
            + + T+PPTAYIT+ T FTNA NVSV+I+FTE C   GGFRC+S N CNLLVYGAGQVVP++L V+EP+LK+ ++VS+S+  +YGR++LVMD NFC+D AGN F R+ NSS ++H+D+R VF D+R H+PE+  Q+ SETRT++ATN+  N++VYLYF+ PI NSSTEIL+SLSIS+G L PI+  + G RRFGF V  IS  AI+T+S  ++  +S QGT ++PVAPVTFLYD QR AVRLSTT RMRT +  IP+ IKF+KPVFGFNSS +SISGG+LQSF E+SRS+Y   IQA DD VSVS+PENVTGDVAGN NL SN+LR++HYT+P IS  +SI  T+ F++T F AGLLT+STASLQSVGA+SRPSS ++SDP R LFR ACHIQ FAL+RWLPVTLPVEYYEFAR ++WS+PYFSLPWE   +   MMG   T   +S+ S+  D  +    +  NK AAA+YG PL+P+EYRS F SQ ++PEA+YI D  +SNGWR+F+RSMFWLAVIGGSLILLHA + F+L+ +K D EK+ SYGAL+FPRFEIFL I+A+PCIC+A+  ++ GG+ +G+ VGILLLG+V+FLLL+LFLFLS+GIT GKLLQYKEVH+ GQ+FHWY+EL+RVTLGPGKRGQWTWKN  +S ++ MFGPLFEDLRGPPKYM+SQI+  N ++    +IA+DDETEDAEAP IQK+FGILRIY+T LE  +R  LGI+AG YL   N SSK+PI++LL +TSFQLFF++LKKPFIKKKVQLVEIIS++CE  +FA C VL+ ++ S   T IGI ML LF I FL+Q+  E YALY QTK+L   +  F +GLK AS+GFLL+F+PQ+L++  ES   +    + +T      CDRN      SS ++R W +Q RE+AK+SFSKD +   +DP
Sbjct:  114 WTVDTIPPTAYITTPTSFTNASNVSVDITFTEPCWSQGGFRCASTNSCNLLVYGAGQVVPNTLNVIEPDLKFSVVVSLSTRDQYGRVILVMDKNFCSDPAGNKFKRTDNSSLFIHFDRRTVFADIRTHIPERQFQIDSETRTVRATNSTENMEVYLYFNEPISNSSTEILNSLSISQGLLTPISVNSFGERRFGFQVRGISQTAIVTLSVRSDLILSWQGTSIAPVAPVTFLYDMQRPAVRLSTTSRMRTCDEQIPVLIKFVKPVFGFNSSHVSISGGQLQSFQEMSRSMYTVNIQARDDFVSVSIPENVTGDVAGNMNLQSNILRLKHYTVPAISEALSILATSAFVVTSFAAGLLTVSTASLQSVGAYSRPSSLMTSDPARNLFRIACHIQFFALTRWLPVTLPVEYYEFARGLQWSVPYFSLPWEMASMHQFMMGPGSTTDPHSYGSKINDFGMKPGKYNVNK-AAALYGLPLSPMEYRSIFGSQDLLPEAQYIVDPKYSNGWRDFNRSMFWLAVIGGSLILLHALVLFVLRLRK-DREKKWSYGALVFPRFEIFLTILAIPCICKASVNVVKGGSSAGIAVGILLLGLVSFLLLALFLFLSIGITLGKLLQYKEVHQVGQKFHWYEELVRVTLGPGKRGQWTWKNSRDSRHIVMFGPLFEDLRGPPKYMLSQIAVGNPNKHPDKVIATDDETEDAEAPCIQKVFGILRIYFTFLEFVKRVCLGIVAGTYL--KNWSSKSPIVVLLTITSFQLFFMLLKKPFIKKKVQLVEIISVACETGIFAACIVLIGRDSSRNETAIGITMLALFFIAFLSQLGNEWYALYRQTKRLGAEDKSFCSGLKAASIGFLLFFIPQRLIRKLESGSALLDRVLKETGDVTSSCDRNRSSGSRSSGTDRPWHKQFRELAKSSFSKDSNVTTSDP 1034          

HSP 2 Score: 95.1301 bits (235), Expect = 2.104e-16
Identity = 46/92 (50.00%), Postives = 56/92 (60.87%), Query Frame = 2

Query:  374 NCDDSQFTLKFFKAPHPFSHLNSAKFVFQVLGAAN-VTCSDCSITCKLDDGSASDCGGRKILYSGLQDGNHSFGVCVNGSQGAACTSYNWTV 646
             CD S+ TLK    P  F++ N A F FQVL   N   C+DCS +CKLDD   + C G ++ Y+ L DGNH+F VC NGS G  C  YNWTV
Sbjct:   25 RCDGSEVTLKLLTTPREFTNRNFANFAFQVLAGGNGDICADCSTSCKLDDYVPAVCEGGEVSYTQLLDGNHTFEVCTNGSLGVGCAHYNWTV 116          

BLAST of g.barbadense_cottongen_reftransV1_0092163 vs. ExPASy TrEMBL
Match: A0A0B2RC29_GLYSO (Uncharacterized protein OS=Glycine soja GN=glysoja_017462 PE=4 SV=1)

HSP 1 Score: 1001.89 bits (2589), Expect = 0.000e+0
Identity = 574/930 (61.72%), Postives = 706/930 (75.91%), Query Frame = 1

Query:  682 TVPPTAYITSSTPFTNALNVSVNISFTEXXXXXXXXXXXXVNDCNLLVYGAGQVVPSSLTVVEPNLKYLLLVSISSASRYGRLVLVMDTNFCTDAAGNPFARSQNSSFYVHYDQRNVFVDLRAHVPEKLLQLRSETRTIQATNNYNNLKVYLYFSAPXXXXXXXXXXXXXXXKGTLLPITGENHGNRRFGFMVXXXXXXXXXXXXFDANSTISRQGTLVSPVAPVTFLYDSQRTAVRLSTTCRMRTREHNIPISIKFMKPVFGFNSSLISISGGRLQSFHEISRSIYAAAIQADDDVVSVSVPENVTGDVAGNKNLASNVLRVRHYTIPXXXXXXXXXXTAIFLLTCFTAGLLTMSTASLQSVGAFXXXXXXXXXDPTRILFRSACHIQIFALSRWLPVTLPVEYYEFARSMEWSIPYFSLPWETGHVQPVMMGSSPTGTSNSFLSRTYDRVISRSFQPN-KIAAAVYGSPLTPVEYRSFFESQSIIPEAEYISDGLHSNGWRNFDRSMFWLAVIGGSXXXXXXXXXXXXKYKKRDSEKQGSYGALIFPRFEIFLVIVALPCICQAAAALIAGGTPXXXXXXXXXXXXXXXXXXXXXXXXXXGITFGKLLQYKEVHREGQQFHWYQELIRVTLGPGKRGQWTWKNEANSVYLTMFGPLFEDLRGPPKYMVSQISG--SNLSRQGGPIIASDDETEDAEAPFIQKLFGILRIYYTMLESARRATLGILAGAYLNNNNTSSKTPIIILLCLTSFQLFFLVLKKPFIKKKVQLVEIISLSCEVAMFATCFVLLDKEFS----TTIGIFMLTLFLIGFLAQMSAEGYALYEQTKQLDTSENKFFTGLKIASVGFLLYFVPQKLMKSFESTFPVFGLCMDTSTTGLPCDRNSSSS-------------ERAWPRQLREMAKASFSKDGSKAPTDP 3411
            T PPTA +T+S  FT++LNVSVNISFTE C GGGGF C SVN CNLLVYGAGQV+PSS  +++PNL Y LLVS+SS  +YGR +LVMD NFCTD AGN F R  NS+ Y+H+D+R V+V++R HVPE+LLQL SETRT+QATN+++ LK+YLYFSAP+LNSSTEIL+S++IS+G+LL    ++ GNRRFGF +ANIS+ AII++ F++ S ISRQGT VSP+APVTFLYD +R AV LST   MRTREHN+ I +KF+KPVFGFNSS ISISGG L+SFHEISRS Y   +QADDD+V +SVPENVT DVAGNKNLASN L+VRHY++P+ISSV+S F TA F+LT   AG LT+STA+LQSVG F+R SS L  DP R L R   HIQ+FALSRWL V LPVE+YEFAR ++W++PYF +PWE   +   M+GS+P G+SN F+++    +  +    +  +AA+VYGSPLT  EY  +FES ++ PEAEY+ D  HS GW  F RSMFWLAVI G L++LHAFL  +LK+ KR+SEK   +GAL FPRFE+FL+ +ALP +C+++  L+ GG+PSG+ VGILLL  V  +LL+LF+FLS+GITFGKLLQYKEVH+EG+ FHWYQEL+RVTLGPGKRGQWTWK +  SV LT+FGPLFEDLRGPPKYM+SQI+G   N   Q   IIASDDETEDAEAPFIQKLFGILRIYY  LES RR +LGILAG ++      SK+P+II+L +TSFQLFF++LKKPFIKK+VQLVEIISL+CEV +FATC VLL K+ S    T  GIFML LFLIG+ AQ++ E YALY Q K LD  E    TGLK AS+GFLLYFVPQK +K  E   P      + +    P D  S +              + AW +QLR  AK+SF ++ S    DP
Sbjct:  118 TTPPTADVTASASFTSSLNVSVNISFTEPCIGGGGFGCKSVNACNLLVYGAGQVIPSSFRIIQPNLTYSLLVSLSSTVQYGRAILVMDRNFCTDRAGNSFMRMPNSTVYIHFDRRKVYVNIRTHVPEELLQLDSETRTVQATNDHDRLKIYLYFSAPVLNSSTEILNSINISQGSLLLNNAKSLGNRRFGFTIANISSTAIISVDFNSESIISRQGTQVSPIAPVTFLYDIKRPAVMLSTY-SMRTREHNLQILVKFVKPVFGFNSSCISISGGLLKSFHEISRSTYMVELQADDDLVFISVPENVTRDVAGNKNLASNFLQVRHYSMPLISSVVSAFATACFVLTSIVAGFLTISTANLQSVGTFTRSSSFLVLDPARNLLRILSHIQVFALSRWLTVKLPVEFYEFARHLQWTVPYFPVPWEAEPMNLFMVGSNPFGSSN-FITKAPATIPKKFLDKSLNLAASVYGSPLTSSEYLQYFESGNMKPEAEYLLDSQHSAGWTEFYRSMFWLAVICGGLMVLHAFLLIVLKFGKRNSEKDKIHGALTFPRFEMFLIFLALPNVCKSSGVLLQGGSPSGLAVGILLLVFVGTVLLALFMFLSIGITFGKLLQYKEVHQEGETFHWYQELVRVTLGPGKRGQWTWKEQPKSVNLTIFGPLFEDLRGPPKYMLSQIAGGSGNPPSQRDCIIASDDETEDAEAPFIQKLFGILRIYYVFLESIRRVSLGILAGLFVLTQ--PSKSPVIIVLSMTSFQLFFMLLKKPFIKKRVQLVEIISLACEVTLFATCLVLLKKDISVSAETKYGIFMLVLFLIGYCAQITNEWYALYIQAKMLDPEEKSLLTGLKNASIGFLLYFVPQKCIKDLEKRLP-----QNDNVNEEPRDTASGADRSRRSSSRSSGTPDGAWLKQLRGFAKSSFGRERSGTRNDP 1038          

HSP 2 Score: 91.2781 bits (225), Expect = 3.419e-15
Identity = 45/92 (48.91%), Postives = 57/92 (61.96%), Query Frame = 2

Query:  374 NCDDSQFTLKFFKAPHPFSHLNSAKFVFQVLGAAN-VTCSDCSITCKLDDGSASDCGGRKILYSGLQDGNHSFGVCVNGSQGAACTSYNWTV 646
             C  S   +KF KAPH FSH  SA F FQV+ + +   CS+C+++CKLDD   S C   K+ Y  L+DGNH+F VC    QG  C +YNWTV
Sbjct:   26 KCGGSDVHVKFLKAPHAFSHSKSASFAFQVINSGSGDPCSNCTLSCKLDDAITSVCTNGKVTYKSLEDGNHTFEVCTR-HQGLGCATYNWTV 116          

BLAST of g.barbadense_cottongen_reftransV1_0092163 vs. ExPASy TrEMBL
Match: A0A068V8N8_COFCA (Coffea canephora DH200=94 genomic scaffold, scaffold_140 OS=Coffea canephora GN=GSCOC_T00005269001 PE=4 SV=1)

HSP 1 Score: 1000.35 bits (2585), Expect = 0.000e+0
Identity = 576/923 (62.41%), Postives = 719/923 (77.90%), Query Frame = 1

Query:  676 IYTVPPTAYITSSTPFTNALNVSVNISFTEXXXXXXXXXXXXVNDCNLLVYGAGQVVPSSLTVVEPNLKYLLLVSISSASRYGRLVLVMDTNFCTDAAGNPFARSQNSSFYVHYDQRNVFVDLRAHVPEKLLQLRSETRTIQATNNYNNLKVYLYFSAPXXXXXXXXXXXXXXXKGTLLPITGENHGNRRFGFMVXXXXXXXXXXXXFDANSTISRQGTLVSPVAPVTFLYDSQRTAVRLSTTCRMRTREHNIPISIKFMKPVFGFNSSLISISGGRLQSFHEISRSIYAAAIQADDDVVSVSVPENVTGDVAGNKNLASNVLRVRHYTIPXXXXXXXXXXTAIFLLTCFTAGLLTMSTASLQSVGAFXXXXXXXXXDPTRILFRSACHIQIFALSRWLPVTLPVEYYEFARSMEWSIPYFSLPWETGHVQPVMMGSSPTGTSNSFLSRTYDRVISRSFQPNK----IAAAVYGSPLTPVEYRSFFESQSIIPEAEYISDGLHSNGWRNFDRSMFWLAVIGGSXXXXXXXXXXXXKYKKRDSEKQGSYGALIFPRFEIFLVIVALPCICQAAAALIAGGTPXXXXXXXXXXXXXXXXXXXXXXXXXXGITFGKLLQYKEVHREGQQFHWYQELIRVTLGPGKRGQWTWKNEANSVYLTMFGPLFEDLRGPPKYMVSQISGSNLSRQGGPIIASDDETEDAEAPFIQKLFGILRIYYTMLESARRATLGILAGAYLNNNNTSSKTPIIILLCLTSFQLFFLVLKKPFIKKKVQLVEIISLSCEVAMFATCFVLLDKEFS----TTIGIFMLTLFLIGFLAQMSAEGYALYEQTKQLDTSENKFFTGLKIASVGFLLYFVPQKLMKSFESTFPVFGLCMDTSTT---GLPCDRNSSSSERAWPRQLREMAKASFSKDGSKAPTDP 3411
            + T+PPTA++T+ST FTNA  V V +SF+E C  GGGF+CSS + CNLLVYGAGQVVP++L V+EPNLK+ L VS+S++ +YGR+V+VMD NFCTD+AGN F R+ NSS Y+H+D+R+VF +LR H+P K+LQ+  E RT+ ATN   NLK+YLYF+ P+LNSS EI+ +++ S G+LLPI G + G RRFGF V +I ++AIIT+SFD+N  +SRQGT VSP+APVTFLYDS R  VRLSTT   RT++ +IP+ IKFMKPVFGFNSS ISISGG L+SF ++S S Y   +QADD  +S+S+PEN+T DVAGN+NL SN+L VRHY++ V+S V+S F+TA F +T   +G LT+STASLQSVGAF RP+  L+S+PTR LFR A HIQ++ALSRWL V+LPVEY EFAR ++WSIPYF LPWET    P M G++      SF S+ +   ++      +     A  VYG PL+P+EYR+FFE QS+IPEAE+I +   ++GWR+F RSMFWLAVI GSL+LLH  LF IL  +K+++EK  SYGALIFPRFEIFL+I+ALPCIC+A+AAL+ GG  SG+VVGILLL VV FLLL+L LFLSVGIT GKLLQYKEVH+EG+  HWYQE+IRVTLGPGKRGQWTWKN++ S  LTM GPLFEDLRGPPKYM+SQI+G N+ +Q   IIASDDETEDAEAPFIQK+FGILRIYYT+LE A+R  LGI+AGAY  + N +S+TP I LLC+TSFQLFF+VLKKPFIKKKVQLVEIIS+SCE  +FATC VLL K FS    T IGIFML+LFL+ FLAQ+  + YALY Q K+LD + N F TGLKIA +GFLL F+PQ+ +K+ ES FP++       TT   G     + SS ER W RQLRE+A++SFSK+ +  PTDP
Sbjct:  117 VDTIPPTAFVTASTNFTNASKVLVYVSFSEPCIDGGGFQCSSPDACNLLVYGAGQVVPNTLNVIEPNLKFSLTVSLSTSVQYGRVVVVMDKNFCTDSAGNSFTRTGNSSLYIHFDRRSVFANLRTHIPAKVLQINREARTVLATNKTKNLKLYLYFTEPVLNSSAEIMKTVNTSLGSLLPIRGNDLGKRRFGFQVKDIPDMAIITVSFDSNQILSRQGTSVSPMAPVTFLYDSLRPTVRLSTTSHTRTKDESIPVLIKFMKPVFGFNSSHISISGGHLESFQKLSTSTYTMDVQADDGSISISIPENITEDVAGNRNLGSNILHVRHYSVSVVSLVLSSFITAAFAVTSLVSGFLTISTASLQSVGAFKRPTPLLTSEPTRSLFRMASHIQVYALSRWLAVSLPVEYSEFARGLQWSIPYFRLPWETLKFPPHMGGTNSPTNPYSFTSKHHYPGVAMDLTSEEQNFLKAPKVYGLPLSPMEYRTFFEGQSMIPEAEHIVNPQTAHGWRDFKRSMFWLAVIYGSLMLLHTLLFSILWLRKKNTEKNRSYGALIFPRFEIFLLILALPCICEASAALLKGGASSGMVVGILLLAVVFFLLLALLLFLSVGITLGKLLQYKEVHQEGEAIHWYQEIIRVTLGPGKRGQWTWKNDSKSTCLTMLGPLFEDLRGPPKYMLSQITGGNVQKQRDRIIASDDETEDAEAPFIQKVFGILRIYYTLLEVAKRVVLGIVAGAY--SENWASRTPTITLLCITSFQLFFMVLKKPFIKKKVQLVEIISVSCEFGIFATCLVLLYKGFSAKDETKIGIFMLSLFLLAFLAQLINQSYALYRQAKKLDPAGN-FLTGLKIALIGFLLLFIPQRWIKNLESKFPLYNTAEGEGTTTSSGDRRSSSRSSDERPWLRQLRELARSSFSKERAATPTDP 1036          

HSP 2 Score: 91.2781 bits (225), Expect = 3.304e-15
Identity = 42/88 (47.73%), Postives = 55/88 (62.50%), Query Frame = 2

Query:  383 DSQFTLKFFKAPHPFSHLNSAKFVFQVLGAANVTCSDCSITCKLDDGSASDCGGRKILYSGLQDGNHSFGVCVNGSQGAACTSYNWTV 646
            +S+  +K  K PH  S  + A F F+V+     +C +C   CKLDDG A+ C  R + Y+ LQDGNH+F VC N SQG  CTSYNW+V
Sbjct:   31 NSEVAVKLLKTPHAVSKRDYATFRFEVVVNGE-SCRECITNCKLDDGLATVCENRSVSYAQLQDGNHTFEVCTNASQGVGCTSYNWSV 117          

BLAST of g.barbadense_cottongen_reftransV1_0092163 vs. ExPASy TrEMBL
Match: R0FN45_9BRAS (Uncharacterized protein OS=Capsella rubella GN=CARUB_v10016613mg PE=4 SV=1)

HSP 1 Score: 998.423 bits (2580), Expect = 0.000e+0
Identity = 573/936 (61.22%), Postives = 715/936 (76.39%), Query Frame = 1

Query:  682 TVPPTAYITSSTPFTNALNVSVNISFTEXXXXXXXXXXXXVNDCNLLVYGAGQVVPSSLTVVEPNLKYLLLVSISSASRYGRLVLVMDTNFCTDAAGNPFARSQNSSFYVHYDQRNVFVDLRAHVPEKLLQLRSETRTIQATNNYNNLKVYLYFSAPXXXXXXXXXXXXXXXKGTLLPITGENHGNRRFGFMVXXXXXXXXXXXXFDANSTISRQGTLVSPVAPVTFLYDSQRTAVRLSTTCRMRTREHNIPISIKFMKPVFGFNSSLISISGGRLQSFHEISRSIYAAAIQADDDVVSVSVPENVTGDVAGNKNLASNVLRVRHYTIPXXXXXXXXXXTAIFLLTCFTAGLLTMSTASLQSVGAFXXXXXXXXXDPTRILFRSACHIQIFALSRWLPVTLPVEYYEFARSMEWSIPYFSLPWETGHVQPVMMGSSPTGTSNSFLSRTYDRVISRSFQPNKIAAAVYGSPLTPVEYRSFFESQSIIPEAEYISDGLHSNGWRNFDRSMFWLAVIGGSXXXXXXXXXXXXKYKKRDSEKQGSYGALIFPRFEIFLVIVALPCICQAAAALI------AGGTPXXXXXXXXXXXXXXXXXXXXXXXXXXGITFGKLLQYKEVHREGQQFHWYQELIRVTLGPGKRGQWTWKNEANSVYLTMFGPLFEDLRGPPKYMVSQISGSN-LSRQGGPIIASDDETEDAEAPFIQKLFGILRIYYTMLESARRATLGILAGAYLNNNNTSSKTPIIILLCLTSFQLFFLVLKKPFIKKKVQLVEIISLSCEVAMFATCFVLLDKEF----STTIGIFMLTLFLIGFLAQMSAEGYALYEQTKQLDTSENKFFTGLKIASVGFLLYFVPQKLMKSFESTFPVFGLCMDTSTTG-----LPCDRNSSSS--------ERAWPRQLREMAKASFSKD--GSKAPTDP 3411
            TV PTA++T+S PFT+A NVSVNI+FTE C GG GFRCSSVN C+LLVYGAGQV+PSSLTV++  LKY LLV +S  ++YGR+VLVM+ N C+D AGN F R+  S F+VH+D+RNVFVDLR HVPEKLL+L ++TRT+QATN+ + L VYLYFS P+LNSSTEIL  LS ++G LLP+ G+ +GNRRF FMV N S  AI+T++ D++S  SR GT  SP AP+TFLYD++R  V L+TT  MRTR+H IP+ IKFMKPVFGFNSS +S+SGG L SF E+S SIY   ++A+   +SV VPENVT DVAGNKNLASN+L V+HY++PV+SSVIS   T IFL+T F AGLLT+ST SL S+GAF RPS  L SDPTR LFR+ACHIQ FALSRWLPVTLPV+YYEF R ++W IPYF LPWET H + +M+ +SP    +S++S+T + +I  + Q +  A +VYG PLT +EYR FFE+ ++ PEAE++    HS  WR+F R MFWLA+IGGSL+LLH FL  ILK+KK  +EK+ S+GA +FPRFE+FL+I+ALP IC+AA +LI       G   + V+VGIL+L VVA LLL+LF+FLS GITFGKLLQYKE+H+EGQ FHWYQELIRVTLGPGKRGQWTWK E NSVYLT  GP+FEDLRGPPKYM++QISGSN L ++   IIASDDE EDAEAP IQKLFGILRIYYT LE+ +R  LGI+AGAY +N   ++KTPI++LL +TSFQLFFLVLKKPFIKKKVQLVEIIS++C+V +FA+C  LL KEF    +  +GIFM+ LFLIGF+AQM  E Y+LY+QTK+LD     F +GLK+  +G     +P K+MK   S  P   L   +++ G      P +R  +SS        ++ W RQ+REMAKASF++D   SK P+DP
Sbjct:  118 TVSPTAFVTASMPFTSAKNVSVNITFTEPCVGGRGFRCSSVNACDLLVYGAGQVIPSSLTVLDQFLKYSLLVGLSPDAQYGRIVLVMNKNVCSDKAGNNFKRALGSRFFVHFDRRNVFVDLRTHVPEKLLKLNNQTRTVQATNDNDKLNVYLYFSEPVLNSSTEILRLLSTNQGDLLPVDGKTNGNRRFAFMVTNTSRRAIVTVTLDSDSIRSRHGTPASPTAPLTFLYDTERPHVLLNTTSGMRTRKHTIPVWIKFMKPVFGFNSSFVSVSGGYLDSFEELSGSIYIVYVKANTSTISVKVPENVTQDVAGNKNLASNILEVKHYSVPVLSSVISWISTYIFLVTSFVAGLLTLSTTSLYSLGAFPRPSPYLISDPTRNLFRTACHIQFFALSRWLPVTLPVDYYEFVRGIQWIIPYFPLPWETKHKEQIMVATSPYIGPHSYISKTQNDMI--NLQTSTNAESVYGLPLTAMEYRLFFETSNLKPEAEHVLGLPHSTVWRDFYRIMFWLAIIGGSLVLLHIFLSLILKFKKAHTEKKRSFGAFVFPRFELFLLILALPSICKAARSLIQGYFKHQGAAEASVIVGILVLCVVAVLLLALFIFLSFGITFGKLLQYKEIHQEGQTFHWYQELIRVTLGPGKRGQWTWKTEKNSVYLTRLGPVFEDLRGPPKYMLTQISGSNPLKQRDDRIIASDDENEDAEAPCIQKLFGILRIYYTFLETVKRVCLGIIAGAYFDNE--TAKTPIVVLLSITSFQLFFLVLKKPFIKKKVQLVEIISIACQVGVFASCLTLLTKEFPEASAKKLGIFMVMLFLIGFIAQMCNEWYSLYKQTKRLDQINRSFLSGLKMFIIGIAALILPHKMMK---SKIPAAQLEGRSNSNGGIPFSTPENRYMNSSGSRSSGSLDKPWLRQIREMAKASFTRDRSNSKVPSDP 1046          

HSP 2 Score: 87.8113 bits (216), Expect = 4.075e-14
Identity = 41/90 (45.56%), Postives = 53/90 (58.89%), Query Frame = 2

Query:  377 CDDSQFTLKFFKAPHPFSHLNSAKFVFQVLGAANVTCSDCSITCKLDDGSASDCGGRKILYSGLQDGNHSFGVCVNGSQGAACTSYNWTV 646
            C  S+ ++KF KAP   S  +SAKF FQ     N TCS C   CKLDD  + DC  R++ Y+ L DG+H+  VC N   G  C +Y+WTV
Sbjct:   27 CYGSELSVKFLKAPPATSRFSSAKFSFQAFEDGNRTCSSCRFHCKLDDRFSFDCHQRRVSYTKLLDGDHTLEVCANKMHGFGCNTYHWTV 116          

BLAST of g.barbadense_cottongen_reftransV1_0092163 vs. ExPASy TrEMBL
Match: V7B0R0_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_009G176900g PE=4 SV=1)

HSP 1 Score: 992.26 bits (2564), Expect = 0.000e+0
Identity = 578/933 (61.95%), Postives = 713/933 (76.42%), Query Frame = 1

Query:  682 TVPPTAYITSSTPFTNALNVSVNISFTEXXXXXXXXXXXXVNDCNLLVYGAGQVVPSSLTVVEPNLKYLLLVSISSASRYGRLVLVMDTNFCTDAAGNPFARSQNSSFYVHYDQRNVFVDLRAHVPEKLLQLRSETRTIQATNNYNNLKVYLYFSAPXXXXXXXXXXXXXXXKGTLLPITGENHGNRRFGFMVXXXXXXXXXXXXFDANSTISRQGTLVSPVAPVTFLYDSQRTAVRLSTTCRMRTREHNIPISIKFMKPVFGFNSSLISISGGRLQSFHEISRSIYAAAIQADDDVVSVSVPENVTGDVAGNKNLASNVLRVRHYTIPXXXXXXXXXXTAIFLLTCFTAGLLTMSTASLQSVGAFXXXXXXXXXDPTRILFRSACHIQIFALSRWLPVTLPVEYYEFARSMEWSIPYFSLPWETGHVQPVMMGSSPTGTSNSFLSRTYDRVISRSFQ--PNK-------IAAAVYGSPLTPVEYRSFFESQSIIPEAEYISDGLHSNGWRNFDRSMFWLAVIGGSXXXXXXXXXXXXKYKKRDSEKQGSYGALIFPRFEIFLVIVALPCICQAAAALIAGGTPXXXXXXXXXXXXXXXXXXXXXXXXXXGITFGKLLQYKEVHREGQQFHWYQELIRVTLGPGKRGQWTWKNEANSVYLTMFGPLFEDLRGPPKYMVSQISG--SNLSRQGGPIIASDDETEDAEAPFIQKLFGILRIYYTMLESARRATLGILAGAYLNNNNTSSKTPIIILLCLTSFQLFFLVLKKPFIKKKVQLVEIISLSCEVAMFATCFVLLDKEFS----TTIGIFMLTLFLIGFLAQMSAEGYALYEQTKQLDTSENKFFTGLKIASVGFLLYFVPQKLMKSFESTFPVFG----LCMDTSTTG----LPCDRNSSSSERAWPRQLREMAKASFSKDGSKAPTDP 3411
            T PPTA++++ST FT++LNVSVNISFTE C GGGGF C SVN CNLLVYGAGQV+PSS  +++PNL Y LLVS+S   ++GR +LVMD NFCTD AGN F R  NSS  +H+D+R V+V++R  VPE+LLQL SETRT+QATN ++ LK+YLYFSAP+LNSSTEIL+S++IS+G+LLP   ++ G+RRFGF+VANIS+ AII+I F++ S I+RQGT VSP+AP+TFLYD+ R AV LST   MRTREHN+ I IKF+KPVFGFNSS ISISGG L+SFHEI R  Y   + A+D +V VSVPENVT DVAGNKNLASN L+VR Y++P+ISSV+S F TA F+LT   AG LT+STASLQS+G F+R SS L  DP R L R   HIQ+FALSRWL V LPVE+YEFA+ ++W+IPYF++PWE+  +   M+GS+P G S         +VI+++    PNK       +AA+VYGSPLT  EY+ +FES+++ PEAEYI D   S+GW  F R MFWLAVI G L++LH FL  +LK+ KR+SEK   +GAL FPRFEIFL+ +ALP IC+++A LI GG+PSG+ VG LL   V  +LL+LFLFLS+GITFGKLLQYKEVH+EG  FHWYQEL+RVTLGPGKRGQWTWK +  SVYLT+FGP+FEDLRGPPKYM+SQISG   N   Q   IIASDDETEDAEAPFIQKLFGILRIYY +LES RR +LGIL+G +++    SSKTP+II+L +TSFQLFF++LKKPFIKK+VQLVEIISL+CEV +FATCF+LL K+FS    T  GIFML LFL+G+ AQ+  E YALY QTK LD  E    TGLK AS+GFLLYF+PQK +K     FP  G       DT++ G    L   R+S + + AW +QLRE AK+S S++ S    DP
Sbjct:  116 TTPPTAHVSASTSFTSSLNVSVNISFTEPCIGGGGFGCKSVNGCNLLVYGAGQVIPSSFRILQPNLTYSLLVSLSPTVQHGRAILVMDKNFCTDLAGNSFMRMPNSSVIIHFDRRKVYVNIRTRVPEELLQLNSETRTVQATNEFDRLKIYLYFSAPVLNSSTEILNSINISQGSLLPHNSKSLGDRRFGFLVANISSTAIISIDFNSESIITRQGTQVSPIAPITFLYDTTRPAVMLSTY-SMRTREHNLQILIKFVKPVFGFNSSCISISGGLLKSFHEIRRDTYMVELLAEDGLVFVSVPENVTRDVAGNKNLASNFLQVRRYSMPLISSVVSAFATASFVLTSIAAGFLTISTASLQSIGTFTRSSSFLVFDPARNLLRILSHIQVFALSRWLAVKLPVEFYEFAKHLQWTIPYFTVPWESETMNLFMVGSNPFGAS---------KVITKAPATIPNKLLVKSLNLAASVYGSPLTSSEYQQYFESENMKPEAEYILDSQPSSGWTEFYRGMFWLAVICGGLMVLHIFLLIVLKFGKRNSEKHRIHGALKFPRFEIFLIFLALPNICKSSAVLIQGGSPSGIAVGTLLFVFVCIVLLALFLFLSIGITFGKLLQYKEVHQEGLTFHWYQELVRVTLGPGKRGQWTWKEQPRSVYLTIFGPMFEDLRGPPKYMLSQISGGTGNPPSQRDRIIASDDETEDAEAPFIQKLFGILRIYYVLLESIRRVSLGILSGLFVSTQ--SSKTPVIIMLSMTSFQLFFMLLKKPFIKKRVQLVEIISLACEVTLFATCFLLLKKDFSVRAETKTGIFMLVLFLVGYCAQIINEWYALYVQTKMLDPEEKSLLTGLKNASIGFLLYFIPQKCIKDLVKRFPENGNGNEESRDTASGGDRSRLSSSRSSGTPDGAWLKQLREFAKSSISRERSGV-NDP 1035          

HSP 2 Score: 85.5001 bits (210), Expect = 1.835e-13
Identity = 44/92 (47.83%), Postives = 54/92 (58.70%), Query Frame = 2

Query:  374 NCDDSQFTLKFFKAPHPFSHLNSAKFVFQVLGAANV-TCSDCSITCKLDDGSASDCGGRKILYSGLQDGNHSFGVCVNGSQGAACTSYNWTV 646
             C  S   +KF K P  FSH NSA F FQVL  ++  TCS+C++ CKLDD     C   K  Y  L+DGNH+F VC    +G  C +YNWTV
Sbjct:   24 KCGGSDVYVKFLKFPRAFSHSNSATFAFQVLNTSSGGTCSNCTLRCKLDDEITRVCKNGKATYRSLRDGNHTFEVCTR-HEGLGCATYNWTV 114