Construction of a high-density linkage map and mapping quantitative trait loci for somatic embryogenesis using leaf petioles as explants in upland cotton (Gossypium hirsutum L.)

Publication Overview
TitleConstruction of a high-density linkage map and mapping quantitative trait loci for somatic embryogenesis using leaf petioles as explants in upland cotton (Gossypium hirsutum L.)
AuthorsXu Z, Zhang C, Ge X, Wang N, Zhou K, Yang X, Wu Z, Zhang X, Liu C, Yang Z, Li C, Liu K, Yang Z, Qian Y, Li F
TypeJournal Article
Journal NamePlant cell reports
Year2015
CitationXu Z, Zhang C, Ge X, Wang N, Zhou K, Yang X, Wu Z, Zhang X, Liu C, Yang Z, Li C, Liu K, Yang Z, Qian Y, Li F. Construction of a high-density linkage map and mapping quantitative trait loci for somatic embryogenesis using leaf petioles as explants in upland cotton (Gossypium hirsutum L.). Plant cell reports. 2015 Mar 11.

Abstract

KEY MESSAGE
The first high-density linkage map was constructed to identify quantitative trait loci (QTLs) for somatic embryogenesis (SE) in cotton ( Gossypium hirsutum L.) using leaf petioles as explants. Cotton transformation is highly limited by only a few regenerable genotypes and the lack of understanding of the genetic and molecular basis of somatic embryogenesis (SE) in cotton (Gossypium hirsutum L.). To construct a more saturated linkage map and further identify quantitative trait loci (QTLs) for SE using leaf petioles as explants, a high embryogenesis frequency line (W10) from the commercial Chinese cotton cultivar CRI24 was crossed with TM-1, a genetic standard upland cotton with no embryogenesis frequency. The genetic map spanned 2300.41 cM in genetic distance and contained 411 polymorphic simple sequence repeat (SSR) loci. Of the 411 mapped loci, 25 were developed from unigenes identified for SE in our previous study. Six QTLs for SE were detected by composite interval mapping method, each explaining 6.88-37.07 % of the phenotypic variance. Single marker analysis was also performed to verify the reliability of QTLs detection, and the SSR markers NAU3325 and DPL0209 were detected by the two methods. Further studies on the relatively stable and anchoring QTLs/markers for SE in an advanced population of W10 × TM-1 and other cross combinations with different SE abilities may shed light on the genetic and molecular mechanism of SE in cotton.

Projects
This publication contains information about 1 projects:
Project NameDescription
WT-F2:3-2015
Germplasm
This publication contains information about 2 stocks:
Stock NameUniquenameType
cdbgm_014964W10breeding_germplasm
cdbpop_000145W10 x TM-1, F2:3population
Featuremaps
This publication contains information about 1 maps:
Map Name
W10 x TM-1, F2:3 (2015)
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN1432-203X
eISSN1432-203X
Publication Date2015 Mar 11
Journal AbbreviationPlant Cell Rep.
LanguageEnglish
Language AbbrENG
Publication TypeJournal Article
Cross References
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DatabaseAccession
PMID: PubMedPMID:25758337