Identification of Molecular Markers Associated with Root-Knot Nematode Resistance in Upland Cotton

Publication Overview
TitleIdentification of Molecular Markers Associated with Root-Knot Nematode Resistance in Upland Cotton
AuthorsNiu C, Hinchliffe D, Cantrell R, Wang C, Roberts P, Zhang J
TypeJournal Article
Journal NameCrop science
Volume47
Issue3
Year2007
Page(s)951-960
CitationNiu C, Hinchliffe D, Cantrell R, Wang C, Roberts P, Zhang J. Identification of Molecular Markers Associated with Root-Knot Nematode Resistance in Upland Cotton. Crop science. 2007; 47(3):951-960.
Publication CodeCRS-47-951

Abstract

Cotton breeding for resistance to root-knot nematode (RKN) [Meloidogyne incognita (Kofoid and White) Chitwood] is hindered by the lack of convenient and reliable screening methods for resistant plants. The identification of molecular markers closely linked to RKN resistance will facilitate the development of RKN resistant cultivars through marker-assisted selection (MAS). Our objective was to identify and develop new DNA markers that are associated with RKN resistance in cotton. Using three pairs of near-isogenic (NIL) resistant (R) and susceptible (S) lines, two AFLP markers, two RAPD markers, and three RGA markers were identified to be polymorphic between the NIL-R and NIL-S lines. One RAPD marker was converted into a sequence-tagged site (STS) marker. In an F2 population of 'ST 474' x 'Auburn 634 RNR', the two RAPD markers and the STS marker were mapped to the same linkage group containing several markers that were previously reported to be linked with the RKN resistance gene rkn1 on chromosome 11 in 'Acala NemX'. All these markers were found to be associated with a major RKN resistance gene, presumably Mi2 in the resistant line Auburn 634 RNR, suggesting that rkn1 and Mi2 are either allelic or closely linked. In addition, no susceptible recombinants were found in a resistance screen of 200 F2 plants from the cross Acala NemX x Auburn 634 RNR. The utility of the two RAPD markers and the converted STS marker were evaluated using 23 R and 8 S germplasm lines. The RAPD and STS markers, along with other previously reported markers associated with RKN resistance will be useful in germplasm screening, MAS for RKN resistance, and map-based cloning for RKN resistance genes.
Features
This publication contains information about 3 features:
Feature NameUniquenameType
UBC781-STSUBC781-STSgenetic_marker
UCR-STSUCR-STSgenetic_marker
UBC693UBC693genetic_marker
Featuremaps
This publication contains information about 1 maps:
Map Name
ST-474 x Auburn 634 RNR, F2 (2007)
Properties
Additional details for this publication include:
Property NameValue
Publication TypeJournal Article
Publication Date2007
Published Location|||
Language Abbreng
Publication Model[electronic resource].
URLhttp://dx.doi.org/10.2135/cropsci2006.07.0499
pISSN0011-183X
LanguageEnglish
Journal CodeCRS
eISSN1435-0653
Publication CodeCRS-47-951
KeywordsGossypium hirsutum, cotton, Meloidogyne incognita, root-knot nematodes, pest resistance, genetic resistance, genetic markers, isogenic lines, amplified fragment length polymorphism, random amplified polymorphic DNA technique, genetic polymorphism, nucleotide sequences, major genes, resistance gene analogue markers, sequence-tagged sites, resistance genes, molecular sequence data
Cross References
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DatabaseAccession
AGL: USDA National Agricultural LibraryAGL:3978809