Development of one set of chromosome-specific microsatellite-containing BACs and their physical mapping in Gossypium hirsutum L.

Publication Overview
TitleDevelopment of one set of chromosome-specific microsatellite-containing BACs and their physical mapping in Gossypium hirsutum L.
AuthorsWang K; Guo W; Zhang T
TypeJournal Article
Journal NameTheoretical and Applied Genetics
Volume115
Issue(5)
Year2007
Page(s)675 682
CitationWang K, Guo W, Zhang T. Development of one set of chromosome-specific microsatellite-containing BACs and their physical mapping in Gossypium hirsutum L. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik. 2007 Sep; 115(5):675-82
Publication CodeTAG-115-675

Abstract

Fluorescence in situ hybridization (FISH), using bacterial artificial chromosome (BAC) clone as probe, is a reliable cytological technique for chromosome identification. It has been used in many plants, especially in those containing numerous small chromosomes. We previously developed eight chromosome-specific BAC clones from tetraploid cotton, which were used as excellent cytological markers for chromosomes identification. Here, we isolated the other chromosome-specific BAC clones to make a complete set for the identification of all 26 chromosome-pairs by this technology in tetraploid cotton (Gossypium hirsutum L.). This set of BAC markers was demonstrated to be useful to assign each chromosome to a genetic linkage group unambiguously. In addition, these BAC clones also served as convenient and reliable landmarks for establishing physical linkage with unknown targeted sequences. Moreover, one BAC containing an EST, with high sequence similarity to a G. hirsutum ethylene-responsive element-binding factor was located physically on the long arm of chromosome A7 with the help of a chromosome-A7-specific BAC FISH marker. Comparative analysis of physical marker positions in the chromosomes by BAC-FISH and genetic linkage maps demonstrated that most of the 26 BAC clones were localized close to or at the ends of their respective chromosomes, and indicated that the recombination active regions of cotton chromosomes are primarily located in the distal regions. This technology also enables us to make associations between chromosomes and their genetic linkage groups and re-assign each chromosome according to the corresponding genetic linkage group. This BAC clones and BAC-FISH technology will be useful for us to evaluate grossly the degree to which a linkage map provides adequate coverage for developing a saturated genetic map, and provides a powerful resource for cotton genomic researches.
Image
File NameLegend
48_FISH_TAG-115-675.jpgTwenty-six individual cotton mitotic chromosomes with FISH signals derived from the chromosome-speciWc BACs, and comparisons of linkage and FISH maps. [Image from Wang 2007, TAG-115-675]
Properties
Additional details for this publication include:
Property NameValue
Publication ModelPrint-Electronic
ISSN0040-5752
Publication Date2007 Sep
Journal AbbreviationTheor. Appl. Genet.
Language Abbreng
Publication TypeJournal Article
Publication TypeResearch Support, Non-U.S. Gov't
Journal CountryGermany
eISSN1432-2242
Journal AliasTheoretical and Applied Genetics. Theoretische und angewandte Genetik
Journal CodeTAG
LanguageEnglish
pISSN0040-5752
Publication CodeTAG-115-675
Published LocationGermany
KeywordsChromosomes, Artificial, Bacterial/*genetics; in-situ hybridization; Chromosomes, Plant/*genetics; Clone Cells; Expressed Sequence Tags; Genetic Markers; Gossypium/*genetics; In Situ Hybridization, Fluorescence; Microsatellite Repeats/*genetics; Minisatellite Repeats/genetics; Physical Chromosome Mapping/*methods; Polyploidy